... lessthan five years, she had become chief operating of cer of the Bay Area’s only automobile manufacturing plant, a U.S Japanese joint venture. She held that job for the better part of a decade and ... of them were “misusing” my theories inan effort to assess and improve the performance of theirleadership teams—and with success!And so it became apparent to me that the five tempta-tions applied ... unafraid to air their dirty laundry. They ad-mit their mistakes, their weaknesses, and their concernswithout fear of reprisal.”Most ofthe staff seemed to be accepting the point, butwithout a...
... that the PAS domain of the phosphorelay histidine kinase Aof Bacillus subtilis wasrecently shown to be a catalytic ATP-binding domain [31].As the PAS domain of BvgS contains a putative ATPbinding ... master regulator of virulence to basic metabolism ofthe bacteria suggests thatthis system may have already been used by an ancestor of the pathogenic bacteria. Virulence genes acquired later inevolution ... After SDS/PAGE analysis the relative amount ofthe phosphorylated histidine kinases was determined by Phos-phorImage analysis. The figure shows representative autoradiographs ofthe samples after...
... stock company size and market share have been small. The shortage of capital is main cause ofthe company’s modest size and market share. Up to now, a multinational join stock company has had only ... The Marketing Strategy ofa multinational join stock companymultinational join stock company has a certain advantage: a multinational join stock company has always kept its prices as competitive ... companyif they take care of their customers, market share and profits will follow. Creating customer values and satisfaction is at the very heart of modern marketing thinking and practice. The...
... stored in a DataTable named ordersDT. • The customersDT and ordersDT DataTable objects are related to each other using the following DataRelation: • DataRelation customersOrdersDataRel = • ... in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable areto be set to the value in the ... meaning that changes tothe CustomerID DataColumn of customersDT are cascaded to ordersDT. This is the default. • None, meaning that changes tothe CustomerID DataColumn of customersDT are...
... chloroplast spinach GAPDH [35] w ere examined t oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... and a lower catalytic constant, whatever the cofactor. The kinetics of the mutant R19 7A were similar to those o f the wild type,while the R 197E mutant had a lower catalytic constant withNADPH....
... definition,there are many hypotheses of latent vectors that are highly related tothe observed words. Therefore,missing words can be used to prune the hypothesesthat are also highly related tothe missing ... this case, many senses will share the same latent semantics profile, as long as they are in the same topic/domain. To solve the sparsity issue we use missing wordsas negative evidence of latent ... link the senses acrossdictionaries, hence Wik is only used as augmenteddata for WMF to better learn the semantics of words.All data is tokenized, POS tagged (Toutanova et al.,2003) and lemmatized,...
... mutagenesis reactions together with oligonucleo-tides ECF-Q69G d(5¢-AACAACGCAGCTGGGCTCTGGAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAACCAGGCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGGCCAGCACGCTAACCAC) and ... Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish to thank H. Steinman for the gift of E. coli OX32 6A. We finally thank MrM. Farrugia for photographic ... normalized to this as a percentage. Aliquots of each SODwere added to sodium azide at the appropriate concentration to yield the required concentration of azide in the complete assay solution, andassayed...
... 5–8). The pK a of Asp140 is much lower than that of Asp142 andGlu144 in all situations where all three residues are presentand the one proton shared by Asp140 and Asp142 appears to remain on Asp142 ... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the sameenzyme. To obtain an insight into ... into account in the calcula-tions (background charges, desolvation penalty and the interaction with other titratable residues), the first factorwas found to be the major determinant ofthe acidity...
... Hierarchy in a modified hiddenMarkov modelLin-Yi ChouUniversity of WaikatoHamiltonNew Zealandlc55@cs.waikato.ac.nzAbstractThis paper explores techniques to take ad-vantage ofthe fundamental difference ... structure of an HHMM The models discussed here are evaluatedby applying them to natural language tasksbased on CoNLL-20041and a sub-corpus of the Lancaster Treebank2.Keywords: information extraction, ... depen-dency value, the model then re-construct the sub-tree at IO parent state P∗moving it to the same level as state N∗, where the states of P∗and N∗now share the same parent, state S.123State...
... literature. Evaluations of subsitemaps of rice and barley a- amylases are thus also presented.MATERIALS AND METHODSSUMAsoftware: subsite mapping of amylasesThis software calculates the apparent binding ... forexample, maltooligosaccharides [15] or a- NP-maltooligo-saccharides [10], the CNP-maltooligosaccharides, which are b-glycosides, are unique as their preparation and use in the mapping ofthe active ... Subsite maps for porcine pancreatic a- amylase (PPA). The solidbars are related to CNP-modified maltooligosaccharide substrates [8]and the open bars depict the subsite map with linear maltooligosac-charides...
... model, then refined smoothly inalternating steps of automatic adjustment with CNS andmanual adjustment with the program O [34]. The finalmodel has a final R-factor of 0.222 with a free R-factor of 0.253. ... Murakawa, M., Takahashi, S., Tsubuki, S.,Kawashima, S., Sakamaki, K. & Yonehara, S. (1998) Purification,molecular cloning, and characterization of TRP32, a novelthioredoxin-related mammalian ... hTRXL-N may accountfor the formation ofa monomer, instead ofa dimer in the case of TRX. Furthermore, the loss of intermoleculardisulfide-bonds and the disbandment ofthe hydrophobicpatch may also...
... the reaction mechanism of a bacterial ATP-citrate lyaseTadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki ImanakaDepartment of Synthetic Chemistry and Biological Chemistry, Graduate ... reaction can be assumed to be the nucleophilic attack of CoA tothe phosphorylated carbonyl carbon of citrylphosphate, and the cleavage ofthe resulting citryl-CoA to acetyl-CoA and oxaloacetate. ... products,AclA (a subunit) and AclB (b subunit). By comparing the primary structures of AclA and AclB with that of the mammalian enzyme, we found that AclA and AclBCorrespondence to T. Imanaka, Department...