... cells, independently of CD81 expression [51,52] The limited involvement of CD81 inthe binding process was further illustrated by the poor binding inhibition achieved inthe presence of mAbs ... N-terminus ofthe NS3 helicase, in two distinct positions of NS4B, inthe centre of NS5A and inthe C-terminal region of NS5B [3–6] The significance of these mutations has been questioned because they ... demonstration of their infectivity in chimpanzees [86–88] To expand thestudies to other genotypes, intergenotypic and intragenotypic chimeras have been constructed replacing the structural proteins of the...
... with the top ofthe goblets held first at cm below the opening ofthe tank for minutes and then at 13 cm for minutes and finally at 20 cm below the opening for minute, before plunging them into the ... decreased binding to FT ZP In line with this argumentation, such decreased binding capacity ofthe ZP may reside in changes ofthe structure ofthe ZP that occur during unprotected freezing and/ ... in viability between fresh and FT spermatozoa may explain the differences in ZP binding registered However, the lack of binding to FT ZP is mainly due to the ZP and not to the spermatozoa, since...
... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G inthe transformed knock-out line does not reach the level ofthe ... conformation ofthe loop His-tags were employed in a topological study ofthe major light harvesting chlorophyll a ⁄ b binding apoprotein, Lhcb1 [18] Interestingly, fusion of a His-tag to the C-terminus, ... light harvesting complex (LHCI), consisting of four members (Lhca1–4) ofthe chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, inthe vicinity ofthe PSI-F and...
... a is the ratio of apparent dissociation constants for binding GST-IjBa inthe presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity ... simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB inthe nucleus when compared with the intensity of NF-jB inthe cell 1686 Recombinant human ... observed inthe untreated cell population In order to shed light on the cause of this result, we carried out an in silico analysis by incorporating the inhibition kinetics within the existing computational...
... black bar Inthe controls 15% ofthe cells were in S-phase, 65% in G0/G1 and 20% in G2/M Inthe following, the S-phase was reduced to 5%, while G0/G1 increased to 73% The proportion of cells in G2/M ... study were the use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated ... controls 15% ofthe cells were in S-phase, 65% in G0/G1 and 20% in G2/M Inthe following, the S-phase was reduced to 5%, while G0/G1 increased to 73% The proportion of cells in G2/M was only minimal...
... and changes in surface morphology by wear The intrinsic factors involve the discoloration ofthe resin material itself, due to the alteration inthe resin matrix and at the interface of matrix ... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering ... influence the final shade ofthe restoration The thickness ofthe composite cores and all-ceramic crown has a significant influence inthe masking ability As the thickness ofthe composite core and the...
... thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] Inthe case of ... (Sf)9 cells expressing the recombinant mPC1 ⁄ 3, the presence ofthe 87 kDa form in excess ofthe 66 ⁄ 71 kDa facilitates isolation ofthe enzyme and helps in maintaining the enzymatic activity ... cooperate inthe activation of an enzyme Inthe case of proPC1 ⁄ 3, removal ofthe various structural and functional domains is a sequential and coordinated event culminating in removal ofthe CT-peptide...
... lines The trend line is a linear fit D) Comparisons of Shannon entropy against categories of incubation The error bars represent the standard deviations ofthe sample entropies Days of incubation ... to new cells of Impact of long-term invitro cell culture on the fidelity of replication of HCV We tested the impact of long-term invitro culturing on CIMM-HCV sequences Samples of PCLB-T4a and ... reversion to the sequence found inthe patient's sera For all three of these changes, one ofthe two isolates had the same base in that position as the patient sample, indicating that the changes...
... Assuming that the same chitin/protein ratio inthe mycelial cords of Hebeloma sp and the fungal component ofthe ectomycorrhizas, then approximately 50% ofthe protein inthe ectomycorrhizas ... Chitin was determined by measuring the amount of fungal glucosamine resulting from hydrolysis of chitin in mycorrhizal roots and mycelial cords using the method of Vignon et al (1986) acid Radiorespirometry ... airflow of 200 ml-min- was maintained and 14 was collected for 90 CO Effluent air was passed directly into a C0 -trap2 ping scintillation fluid containing an organic amine (Carbomax, Kontron) in 10...
... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins inthe whole ... sequences in GenBank (except in an independent sequence ofthe Frankfurt strain), and in none of SARSlike bat-CoV sequenced inthe ORF 7b region (Figure 1) To determine whether the deletion originated ... transcription, confirming earlier findings [50] Induction of transcription was not observed with r7bΔTMD either, indicating that the ORF 7b protein is not involved inthe Page of 17 (page number...
... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins inthe whole ... sequences in GenBank (except in an independent sequence ofthe Frankfurt strain), and in none of SARSlike bat-CoV sequenced inthe ORF 7b region (Figure 1) To determine whether the deletion originated ... transcription, confirming earlier findings [50] Induction of transcription was not observed with r7bΔTMD either, indicating that the ORF 7b protein is not involved inthe Page of 17 (page number...
... solution Loading ofthe blastocysts into the straw was performed using the capillary effect by simply touching the to µl droplet, containing the blastocysts with the narrow end ofthe pulled straw ... This end was then immediately submerged into liquid nitrogen Warming was performed by immersing the end ofthe straw containing the blastocysts into 1.2 ml of holding medium containing 0.25 M sucrose ... min, the blastocysts were transferred into 1.2 ml of holding medium containing 0.12 M sucrose for another and then twice for Finally the blastocysts were transferred twice for each into holding...
... http://genomebiology.com/2005/6/13/R109 Comparing the transcriptomes ofinvitro senescence and aging We hypothesised that if senescence is an important mechanism of aging in mammals, then the expression profile of cellular senescence ... and nk indicate the number of chips inthe j and k groups Thus, the geometric mean ofthe expression measures from the aging (or senescent) samples was divided by the geometric mean ofthe expression ... groups on the basis ofthe nature ofthe comparison: aging versus aging (n = 21), aging versus senescence (n = 28), and senescence versus senescence (n = 6) The aging versus aging group is further...
... could not confirm the impact of systemic changes of T-cell phenotype to the nasal mucosal inflammation following SIT therapy In conclusion, our in vivo and invitrostudies presented the evidence ... 54 weeks and then tri-weekly for 27 weeks (Fig S1) The adjustment inthe dosing schedule was mainly based on the tolerance of dose and the occurrence of adverse reactions None ofthe study patients ... were not included inthe molecular data analysis of this study (Fig 1) Therefore, the samples from the twenty patients with significant response to the treatment were used inthe following in vivo...
... bottles of wine were/*was fermenting (The/ A lot of) wine was fermenting Two bottles of wine were/*was spilling (A lot of) wine was spilling The two bottles of Argentinian wine were/*was delicious The ... amount of debate The aspects dealt with in part I1 predominantly concern the internal structure of noun phrases containing two nominal elements At the heart ofthe discussions in this part is the ... in view ofthe number of ‘mismatches’ between the (syntactic) number ofthe head of a construction and the number ofthe finite verb, as well as the variety of constructions in which these mismatches...
... targets but these were often not put into the public domain Now consider the loss of institutional memory as leaders in these areas left the company either as a result of early retirement or attractive ... find the published results of translational research performed by others than it is to find the unpublished results of translational research done within your own institution, especially if the ... Marincola: Create a translational medicine knowledge repository - Research downsizing, mergers and increased outsourcing have reduced the depth of in- house translational medicine expertise and institutional...
... candidate inthe Department of Business Administration at the University of Illinois at Urbana-Ghampaign Beginning inthe fall of 2002, she will be an assistant professor of management at the University ... from their parents All of these findings suggest that support by significant others outside individuals' workplace can influence their responses at work, including their creativity But much ofthe ... on the creativity of adult employees and if this impact is independent ofthe impact of support from the current work and nonwork sources investigated in this study Finally, inquiries into the...
... experiment was performed inthe absence ofthe compound to determine the amount of lipid peroxidation obtained inthe presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... (a) The presence ofthe methoxy group inthe seven member ring may enhance the stability ofthe nitrogen centred radical due to the electron conjugation effect The absence of N– Evaluation ofIn ... absent ofthe N–H absorption band at 3400 cm–1 and the presence ofthe C=O stretching band at 1600 cm–1, whereas in compounds (a) and (d), the presence of N–H stretching and the absence of C=O...
... sealed end first, into liquid nitrogen to cover the embryos The rest ofthe straw was then slowly immersed into the liquid nitrogen within 30 sec Inthe vitrified control group, the embryos were ... among the factors influencing cryosurvival of embryos The incorporation of DMSO into an EG-containing medium has at least two advantages Firstly, vitrification is facilitated because ofthe greater ... by blocking monomer addition at the fastgrowing end ofthe polymers [19], thus preventing cytokinesis without affecting karyokinesis [4,13] Treatment of cells with cytochalasins makes the plasma...
... shows the length ofthe longest shoot In wild cherry, the cold clearly improved the growth ofthe shoots, independently ofthe time of storage As the storage time increased, the growth ofthe shoots ... 1985; Marino et al, 1985) Several factors appear to regulate theof cold storage ofinvitro cultures: the physiological state of shoots, the type of explant, the medium, the container, the temperature ... the percentage of viability and the latter only with the growth of live explants The results obtained are shown in figure The marked influence ofthe time of transfer to storage on the multiplication...