the contributions of in vitro studies

... cells, independently of CD81 expression [51,52] The limited involvement of CD81 in the binding process was further illustrated by the poor binding inhibition achieved in the presence of mAbs ... N-terminus of the NS3 helicase, in two distinct positions of NS4B, in the centre of NS5A and in the C-terminal region of NS5B [3–6] The significance of these mutations has been questioned because they ... demonstration of their infectivity in chimpanzees [86–88] To expand the studies to other genotypes, intergenotypic and intragenotypic chimeras have been constructed replacing the structural proteins of the...

Ngày tải lên: 16/03/2014, 05:20

... with the top of the goblets held first at cm below the opening of the tank for minutes and then at 13 cm for minutes and finally at 20 cm below the opening for minute, before plunging them into the ... decreased binding to FT ZP In line with this argumentation, such decreased binding capacity of the ZP may reside in changes of the structure of the ZP that occur during unprotected freezing and/ ... in viability between fresh and FT spermatozoa may explain the differences in ZP binding registered However, the lack of binding to FT ZP is mainly due to the ZP and not to the spermatozoa, since...

Ngày tải lên: 12/08/2014, 18:22

... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level of the ... conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding apoprotein, Lhcb1 [18] Interestingly, fusion of a His-tag to the C-terminus, ... light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...

Ngày tải lên: 07/03/2014, 21:20

... a is the ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity ... simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in the nucleus when compared with the intensity of NF-jB in the cell 1686 Recombinant human ... observed in the untreated cell population In order to shed light on the cause of this result, we carried out an in silico analysis by incorporating the inhibition kinetics within the existing computational...

Ngày tải lên: 16/03/2014, 11:20

... black bar In the controls 15% of the cells were in S-phase, 65% in G0/G1 and 20% in G2/M In the following, the S-phase was reduced to 5%, while G0/G1 increased to 73% The proportion of cells in G2/M ... study were the use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated ... controls 15% of the cells were in S-phase, 65% in G0/G1 and 20% in G2/M In the following, the S-phase was reduced to 5%, while G0/G1 increased to 73% The proportion of cells in G2/M was only minimal...

Ngày tải lên: 09/08/2014, 09:22

... and changes in surface morphology by wear The intrinsic factors involve the discoloration of the resin material itself, due to the alteration in the resin matrix and at the interface of matrix ... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering ... influence the final shade of the restoration The thickness of the composite cores and all-ceramic crown has a significant influence in the masking ability As the thickness of the composite core and the...

Ngày tải lên: 09/10/2015, 11:24

... thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the case of ... (Sf)9 cells expressing the recombinant mPC1 ⁄ 3, the presence of the 87 kDa form in excess of the 66 ⁄ 71 kDa facilitates isolation of the enzyme and helps in maintaining the enzymatic activity ... cooperate in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a sequential and coordinated event culminating in removal of the CT-peptide...

Ngày tải lên: 30/03/2014, 08:20

... lines The trend line is a linear fit D) Comparisons of Shannon entropy against categories of incubation The error bars represent the standard deviations of the sample entropies Days of incubation ... to new cells of Impact of long-term in vitro cell culture on the fidelity of replication of HCV We tested the impact of long-term in vitro culturing on CIMM-HCV sequences Samples of PCLB-T4a and ... reversion to the sequence found in the patient's sera For all three of these changes, one of the two isolates had the same base in that position as the patient sample, indicating that the changes...

Ngày tải lên: 20/06/2014, 02:20

... Assuming that the same chitin/protein ratio in the mycelial cords of Hebeloma sp and the fungal component of the ectomycorrhizas, then approximately 50% of the protein in the ectomycorrhizas ... Chitin was determined by measuring the amount of fungal glucosamine resulting from hydrolysis of chitin in mycorrhizal roots and mycelial cords using the method of Vignon et al (1986) acid Radiorespirometry ... airflow of 200 ml-min- was maintained and 14 was collected for 90 CO Effluent air was passed directly into a C0 -trap2 ping scintillation fluid containing an organic amine (Carbomax, Kontron) in 10...

Ngày tải lên: 09/08/2014, 04:20

... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins in the whole ... sequences in GenBank (except in an independent sequence of the Frankfurt strain), and in none of SARSlike bat-CoV sequenced in the ORF 7b region (Figure 1) To determine whether the deletion originated ... transcription, confirming earlier findings [50] Induction of transcription was not observed with r7bΔTMD either, indicating that the ORF 7b protein is not involved in the Page of 17 (page number...

Ngày tải lên: 12/08/2014, 04:20

... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins in the whole ... sequences in GenBank (except in an independent sequence of the Frankfurt strain), and in none of SARSlike bat-CoV sequenced in the ORF 7b region (Figure 1) To determine whether the deletion originated ... transcription, confirming earlier findings [50] Induction of transcription was not observed with r7bΔTMD either, indicating that the ORF 7b protein is not involved in the Page of 17 (page number...

Ngày tải lên: 12/08/2014, 04:20

... solution Loading of the blastocysts into the straw was performed using the capillary effect by simply touching the to µl droplet, containing the blastocysts with the narrow end of the pulled straw ... This end was then immediately submerged into liquid nitrogen Warming was performed by immersing the end of the straw containing the blastocysts into 1.2 ml of holding medium containing 0.25 M sucrose ... min, the blastocysts were transferred into 1.2 ml of holding medium containing 0.12 M sucrose for another and then twice for Finally the blastocysts were transferred twice for each into holding...

Ngày tải lên: 12/08/2014, 15:20

... http://genomebiology.com/2005/6/13/R109 Comparing the transcriptomes of in vitro senescence and aging We hypothesised that if senescence is an important mechanism of aging in mammals, then the expression profile of cellular senescence ... and nk indicate the number of chips in the j and k groups Thus, the geometric mean of the expression measures from the aging (or senescent) samples was divided by the geometric mean of the expression ... groups on the basis of the nature of the comparison: aging versus aging (n = 21), aging versus senescence (n = 28), and senescence versus senescence (n = 6) The aging versus aging group is further...

Ngày tải lên: 14/08/2014, 16:20

... could not confirm the impact of systemic changes of T-cell phenotype to the nasal mucosal inflammation following SIT therapy In conclusion, our in vivo and in vitro studies presented the evidence ... 54 weeks and then tri-weekly for 27 weeks (Fig S1) The adjustment in the dosing schedule was mainly based on the tolerance of dose and the occurrence of adverse reactions None of the study patients ... were not included in the molecular data analysis of this study (Fig 1) Therefore, the samples from the twenty patients with significant response to the treatment were used in the following in vivo...

Ngày tải lên: 23/09/2015, 14:47

... bottles of wine were/*was fermenting (The/ A lot of) wine was fermenting Two bottles of wine were/*was spilling (A lot of) wine was spilling The two bottles of Argentinian wine were/*was delicious The ... amount of debate The aspects dealt with in part I1 predominantly concern the internal structure of noun phrases containing two nominal elements At the heart of the discussions in this part is the ... in view of the number of ‘mismatches’ between the (syntactic) number of the head of a construction and the number of the finite verb, as well as the variety of constructions in which these mismatches...

Ngày tải lên: 27/10/2016, 12:27

... targets but these were often not put into the public domain Now consider the loss of institutional memory as leaders in these areas left the company either as a result of early retirement or attractive ... find the published results of translational research performed by others than it is to find the unpublished results of translational research done within your own institution, especially if the ... Marincola: Create a translational medicine knowledge repository - Research downsizing, mergers and increased outsourcing have reduced the depth of in- house translational medicine expertise and institutional...

Ngày tải lên: 18/06/2014, 19:20

... candidate in the Department of Business Administration at the University of Illinois at Urbana-Ghampaign Beginning in the fall of 2002, she will be an assistant professor of management at the University ... from their parents All of these findings suggest that support by significant others outside individuals' workplace can influence their responses at work, including their creativity But much of the ... on the creativity of adult employees and if this impact is independent of the impact of support from the current work and nonwork sources investigated in this study Finally, inquiries into the...

Ngày tải lên: 09/07/2014, 08:25

... experiment was performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... (a) The presence of the methoxy group in the seven member ring may enhance the stability of the nitrogen centred radical due to the electron conjugation effect The absence of N– Evaluation of In ... absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, whereas in compounds (a) and (d), the presence of N–H stretching and the absence of C=O...

Ngày tải lên: 07/08/2014, 14:20

... sealed end first, into liquid nitrogen to cover the embryos The rest of the straw was then slowly immersed into the liquid nitrogen within 30 sec In the vitrified control group, the embryos were ... among the factors influencing cryosurvival of embryos The incorporation of DMSO into an EG-containing medium has at least two advantages Firstly, vitrification is facilitated because of the greater ... by blocking monomer addition at the fastgrowing end of the polymers [19], thus preventing cytokinesis without affecting karyokinesis [4,13] Treatment of cells with cytochalasins makes the plasma...

Ngày tải lên: 07/08/2014, 23:22

... shows the length of the longest shoot In wild cherry, the cold clearly improved the growth of the shoots, independently of the time of storage As the storage time increased, the growth of the shoots ... 1985; Marino et al, 1985) Several factors appear to regulate the of cold storage of in vitro cultures: the physiological state of shoots, the type of explant, the medium, the container, the temperature ... the percentage of viability and the latter only with the growth of live explants The results obtained are shown in figure The marked influence of the time of transfer to storage on the multiplication...

Ngày tải lên: 08/08/2014, 19:21