... capable of giving strength to the weary, vivacity to the stupid, and wisdomto men void of understanding; capable of soothing the sorrows ofthe afflicted, of healing the wounds of the spirit, and ... healthycondition ofthe vessels belonging to the membranes ofthe socket, with the condition of which, the state of the tooth is closely connected."]Having gone through with an examination ofthe physical ... towards them.Hence the Methodists interdicted the common use of tobacco with that of ardent spirit, in the infancy of theirsociety; thereby evincing a just sense ofthe self-denial, decency, and...
... difficult. The oldest or the husband ofthe family sits at the head ofthe table and others sit in descendingorder according to age or wherever they like. The meal starts when everyonehas their ... the serving dishes and add them to the rice. Then, they hold the rice bowl near their mouth, using their chopsticks toeat. However, eating on the table sometimes is not easy as there are standards and ... concepts andthe whole knowledge ofthe world. His idea about the influence of language upon thought, andthe existence of an “intermediateuniverse” of concepts interposed between man andthe universe,...
... spectrophotometric methods.Because the enzymatic activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, ofthe activity of HPRT, the role of the PRTFDC1 in purine metabolism ... II. The hood domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... interacts with bothO6 and N7 (Fig. 1C). The 2¢OH ofthe ribose is hydro-gen bonded to the main chain andthe side chain of Asp142 (Fig. 1C). Both hydroxyl groups ofthe riboseare involved...
... position at the present day. The present theories ofthe transmission of light and sound; ofthe production of winds, and sun-spots, andofthe method of development and dissemination of heat, ... These theories throw new light upon the character and extent ofthe atmosphere ofthe moon and planets, andthe consequent availability of those and other spheres for sustaining life. The ... stored the coal in the bosom ofthe earth, and piled up the polar ice. He it is that aids the chemist, drives the engine, ripens the harvest, dispenses life and health. The study ofthe sun and...
... fact, the ornament of most ofthe examples of Praetollia rnaynci, the type species, is like that ofthe inner whorls of Tollia and we do not know whcthcr the specimens were adults or the ... of the section on page 8) of Lowcr Volgian age; and an Upper IIartzfjdd Sandstone, of early Cretaceolis date, comprising the remainder ofthe formation. In south-western Jameson Land the ... parts ofthe course of thc Volga, and NIKITIN'S record frorrr Vir,gntus Zone, is, doubtless, dne to the fact that the higher zoncs of the Lower Volgian had not then been separated. In the...
... depend on the spe-cifics ofthe ACP program in effect during the period covered by the data used to estimate the parameters ofthe model. (By specifics we mean, for example, the amount ofthe annual ... Warner in response to the creation of the DRM by Glenn Gotz and John McCall. At the time the DRM was originally created it was pushing the limits ofthe capabilities of even the fastest computers. ... R-3028-AF, 1984, andthe extension ofthe basic DRM to take into account the effect ofthe availability of multiyear contracts to certain classes of Air Force officers. Unlike other models, the DRM takes...
... characteristics ofthe Internet;do, Ko and DI Koare defined as the average diameter, K,andDI K ofthe original networks and df,Kf and DI Kfdenote the diameter, K,andDIK after 10% ofthe nodes ... meaningful. The other metric, S, is definedas the ratio ofthe number of nodes in the giant connectedcomponent divided by the total number of nodes. One mightnotice the different characteristics ofthe ... severalsnapshots ofthe real Internet topology.1On the other hand,[42], [43] have shown that the clustering coefficient of the Internet has been growing and that the average diameter of the Internet...
... Superposition ofthe active-siteresidues ofthe 20 conformers ofthe solu-tion structure of HtA. Catalytic groups E66 and H113 are shown in red, and side chains of other residues in their vicinity ... understanding the structural requirements for the general ribonucleolytic and cytotoxic activities ofthe protein HtA. With thisaim, we determined the structure of HtA by1H-NMRmethods, andthe ... Comparison ofthe spatial orientation of the N-terminal b-hairpin and loops 2 and 5 in HtA and a-sarcin. The backbone trace isrepresented in blue for the b-hairpin, orangefor loop 5, and green...
... significantlyincreases the cost ofthe purification, andthe require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian ... Subramanian and Shree[42], there was no rigorous assessment ofthe purity of the product or the correctness ofthe sequence. In con-trast, the purification protocol that we have developedis quick and ... line) and 9 lM (triangles, dashed line). Each datapoint is the mean of eight replicates ± the standard error; where error bars are not visible, the standard error was smaller than the size of the...
... interruption ofthe geometry of the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data ofthe C-terminal portion of helix III ... consistent with the NOE trend ofthe correspondingadjacent residues and hence does not conflict with the global interpretation ofthe data. With the exclusion of the N-terminal octapeptidyl and C-terminal ... Lorentzian model of J(x) calculated over avery wide range of correlation times, s. Most of the experimental points accumulate rather close to the upper intercept ofthe theoretical curve andthe fitting(solid)...
... analyses ofthe predictedamino acid sequence showed that the C-terminal half of the RepA ofthe pIT3 plasmid is sequence-similar to the helicases ofthe phage-encoded superfamily III pro-teins. The ... of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and thenrecording the CD spectra ofthe incubated samples ... DNArecognition and binding and is positioned left of the active site [14].Rep245 is both capable of de novo synthesis of DNA primers andof elongating them. Long DNAextension products were observed on the...
... (Met1)-ONC(M23L) by the addition of AAP, as described in the Materials and methods. Completion ofthe hydrolysis wasconfirmed by the disappearance ofthe (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS. The addition ofthe protease is considered the zero time point. The relative intensities ofthe peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC (M23L), ... simplify the protocol and shorten the time needed to obtain a fully active ONC compared withother procedures described in the literature. The application of MALDI-TOF MS to the characterization of...