the causative agent trypanosoma cruzi
Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family ppt
Ngày tải lên :
15/03/2014, 00:20
... contacts with their equivalents in the other subunit of the second dimer (Fig 4A) In the case of P21212 (TcRpiB-R5P), the four molecules in the asymmetric unit represent the tetramer The two active ... in the biological cycle of the parasite (i.e the epimastigote and the metacyclic trypomastigote in the insect vector, and the intracellular amastigote and the bloodstream trypomastigote in the ... for other complex structures The phosphate group lies at the same place, although the rest of the sugar is much less well defined The electron density suggests that All6P is bound primarily as the...
- 16
- 401
- 0
Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
Ngày tải lên :
23/03/2014, 15:20
... accounts for the majority of the sulfated sugar In conclusion, the results obtained provide evidence of the nature of the glycans present in the unique N-glycosylation site present in the C-terminal ... analysis to characterize the N-linked glycans present in the protein In this paper we report, for the first time, the presence of sulfate in the N-linked oligosaccharides of cruzipain This structural ... inset in Fig 6B) Altogether, these data confirm the presence of sulfated high-mannose type oligosaccharides in cruzipain and in its C-terminal domain This is the first report of the use of nor-harmane...
- 13
- 536
- 0
Báo cáo y học: "Trypanosoma cruzi (Chagas'''' disease agent) reduces HIV-1 replication in human placenta" pps
Ngày tải lên :
13/08/2014, 05:21
... America, one of the most important endemic protozoonoses is Chagas' disease, caused by the protozoan parasite Trypanosoma cruzi It extends from southern USA to southern South America There are approximately ... with the proviral pNL-LucE-R+ vector [58], which lack the env gene and has the firefly luciferase gene inserted into the nef gene, and the expression vector pCMV harboring the gene coding for either ... pseudotyped virus Although we not address the effects of T cruzi on the binding of HIV-1 to their natural receptors, our experiments are valid in studying the effects of the parasite on viral replication...
- 13
- 220
- 0
Tài liệu Báo cáo khoa học: A FYVE-containing unusual cyclic nucleotide phosphodiesterase from Trypanosoma cruzi docx
Ngày tải lên :
19/02/2014, 07:20
... the interaction of the FYVE-type domain with a target membrane On the other hand, the dimerization will also entail a dimerization of the downstream catalytic domains, and may thus influence their ... directs the expression of the genuine protein, while the other adds an N-terminal hemagglutinin-tag to facilitate detection of the recombinant proteins Transformation of the constructs into the PDE-deficient ... lysine The consequence of these replacements is a decrease of the overall net charge of the motive from +4 to +1 The effect of these changes on a putative membrane binding of the FYVE-type domain...
- 11
- 479
- 0
Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
Ngày tải lên :
19/02/2014, 07:20
... observed that the addition of ornithine or arginine to the culture medium cannot support the continuous growth of T cruzi in the defined medium, as proliferation in the presence of these amino acids ... gave the results predicted The radioactive bands obtained in the corresponding Southern blot assay (Fig 3B) could only be explained by the integration, into the parasite genome, of ‡ units of the ... caused by the fact that the restriction enzyme, BstBI, only produced partial digestion of the DNA samples On the other hand, the absence of a second hybridization band in lane might be the result...
- 10
- 570
- 0
Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
Ngày tải lên :
19/02/2014, 16:20
... measured together in the same assay The data indicate that the presence of propionate in the medium reduces the activity by % 50% in the wild-type as well as in the DmcsA strain Therefore it ... grown only on glucose there was no significant difference between the wild-type and the DmcsA strain In the presence of only acetate there was no difference between the strains; the approximate growth ... compensates for the initial decrease of the oxaloacetate concentration in the equilibrium due to the accumulation of NADH [25] Lineweaver–Burk diagrams were obtained by use of the worksheet of the program...
- 15
- 678
- 0
Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
Ngày tải lên :
20/02/2014, 02:21
... has the closest similarity to the substrate 1,3-BPGA, keeping the overall size, the two phosphoryl moieties, the carbonyl at the C2 position and the (R) configuration at the C3 carbon bearing the ... were calculated from the slopes of the curves recorded during the first of the reaction and from the NADH concentrations using the value e340 ¼ 6.22 mM)1Æcm)1 Inhibition studies The inhibitory activities ... preincubation of the enzyme with the compound for followed by addition of the reaction mixture to start the reaction A possible effect of the inhibitors on the absorbance of NADH was checked The concentration...
- 13
- 588
- 0
Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
Ngày tải lên :
15/03/2014, 23:20
... in Trypanosoma cruzi F B Holetz et al Introduction The life cycle of the three trypanosomatid parasites pathogenic to humans involves the alternation between a mammal and an arthropod host The ... • • • • any of the corresponding mRNAs in the SP sample, demonstrating the specific presence of these mRNAs in the IP sample Moreover, mRNAs from other large gene families in T cruzi, e.g those ... analysis of the human pathogen Trypanosoma cruzi Proteomics 4, 1052–1059 30 Atwood JA, Weatherly DB, Minning TA, Bundy B, Cavola C, Opperdoes FR, Orlando O & Tarleton RL (2005) The Trypanosoma cruzi...
- 12
- 481
- 0
Báo cáo Y học: The trans-sialidase from the African trypanosome Trypanosoma brucei potx
Ngày tải lên :
31/03/2014, 23:20
... demonstrated that the substitution Q284-P in TrSA increased significantly the hydrolytic activity of the enzyme The three other positions in the neighbourhood of the active site that differ between trypanosomal ... capable of hydrolysing the a-(2,6) regioisomer, losing the strict specificity of the wild-type enzyme for the sialyla-(2,3) substrate (Fig 5) The active sites of the T brucei and T cruzi trans-sialidases ... to the TcTS lectin-like domain TbTS does not have a repetitive domain at the C-terminus that is homologous to the T cruzi SAPA domain The catalytic region revealed the conservation of most of the...
- 10
- 292
- 0
Báo cáo khoa học: "Disintegration of Dung Pats from Cattle Treated with the Ivermectin Anthelmintic Bolus, or the Biocontrol Agent Duddingtonia flagrans" ppsx
Ngày tải lên :
12/08/2014, 15:20
... lifting the nylon mesh supporting the dung pat from the ground on to a field scale and then replacing as close as possible to the same alignment on the sub-plot Visual examination of the plot ... of the faecal pats Prior to deposition of the pats, the pasture was mowed to approximately cm The plot area was divided into 48 separate 80×80 cm subplots with 20 cm buffer zones between the ... Larsen 2000) The resting spores (chlamydospores) of the fungus are capable of surviving the gut passage in ruminants and with warm and moist conditions they germinate and spread in the faecal deposits...
- 10
- 192
- 0
Báo cáo y học: "Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi" pot
Ngày tải lên :
14/08/2014, 20:22
... [19] Protozoan parasites of the family Trypanosomatidae are the causative agents of African sleeping sickness (Trypanosoma brucei), American trypanosomiasis (Trypanosoma cruzi) http://genomebiology.com/2007/8/3/R37 ... 150 kb upstream of the putative centromere (c) Comparison of the T cruzi chromosome centromeric domain with the syntenic region of T brucei chromosome In the T cruzi chromosome, the degenerate VIPER/SIRE ... indicate putative ORFs and the implied direction of transcription The dashed lines between the T cruzi and T brucei maps identify the equivalent positions of the first ORFs of the conserved directional...
- 14
- 296
- 0
1086 the causative
Ngày tải lên :
25/08/2016, 22:10
... MOTHER THE PLANTS WATERED (PRESENT CONTINUOUS) 6- SHE ……………………………… THE TICKETS FOR HER HOLIDAYS BOUGHT (FUTURE PERFECT) COMPLETE THE SENTENCES WITH THE CAUSATIVE FORM USE THE TENSE GIVEN 1- THEY ... REWRITE THESE SENTENCES 1- THEY ARE MOVING THEIR NEW FURNITURE TODAY THEY 2- THE MAID CLEANS TOM’S HOUSE ONCE A WEEK TOM 3- THE DRESSMAKER MADE PAM A NEW DRESS FOR THE ... /INSTALL) (GOING TO) 5- I (THE COMPUTER /REPAIR) (PAST PERFECT) 6- MY MOTHER (THE MEAL/COOK) EVERYDAY (PRESENT SIMPLE) 7- THE CHILDREN……………… (THEIR ROOMS/ TIDY) AT THE MOMENT THEY ARRIVED (PAST CONTINUOUS)...
- 3
- 212
- 0
Tài liệu THỂ SAI BẢO (Causative form) pdf
Ngày tải lên :
14/12/2013, 17:16
... Chủ động: S + HAVE + O + BARE INF Bị động : S + HAVE + O + P.P Ví dụ: I have them build my house.(chủ động ) I have my house built (bị động ) Ngoài động từ GET dùng thể sai ... thức có khác thể chủ động Chủ động : S + GET + O + TO INF Bị động: S + GET + O + P.P Ví dụ: I get them to repair my house.(chủ động ) I get my house repaired (bị động ) USED TO 1.USED TO : ( Đã ... : S + USED TO + INF - Để hành động xảy khứ mà không I used to smoke : hút thuốc ( không hút ) There used to be a river here : có sông 2.BE/GET USED TO (quen ) CÔNG THỨC : S (người ) + GET/BE...
- 4
- 830
- 5
Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc
Ngày tải lên :
20/02/2014, 11:20
... The capacity of the drug to react with the genomic DNA is inversely proportional to the concentration of GSH The higher the GSH level, the lower the amount of drug–DNA adduct and vice versa The ... the Fig 7) are detected and correspond to the reaction of the drug with methanol used to wash the needle of the quadrupole just prior to the injection The asymmetric compound, S28053-1, was then ... but the pure enantiomeric forms S27590-1 and S27589-1, which are the two cis enantiomers with the acetate groups located either above or below the plane of the aromatic chromophore (Fig 1) These...
- 12
- 701
- 0
Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
Ngày tải lên :
21/02/2014, 01:21
... h with the indicated NAMI-A concentration (NAMI-A) Cells were then stimulated for h with medium M199 containing either 100 nM PMA (B) or 10% fetal bovine serum (C), in the presence of the indicated ... expression To establish whether the decrease in c-myc mRNA expression elicited by NAMI-A might have occurred at the transcriptional level, we assessed the rate of transcription of the c-myc gene by using ... the immunoblotting analysis correspond to c-myc immunoreactivity are shown in the panel B ECV304 cells and that such a condition plays a key role in the alteration of the growth behavior of these...
- 10
- 703
- 0
Báo cáo khoa học: The propeptide of cruzipain ) a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F ppt
Ngày tải lên :
16/03/2014, 11:20
... cathepsins F and W share the same degree of similarity to the prodomain of cruzipain, the similarity of the mature domain of cathepsin F to that of cruzipain is higher (48%) than that between the ... with the crystal structure of mature cruzipain The modeled PCZ–cruzipain complex was then compared with the experimental structural data available for the other enzymes, particularly surveying the ... changes the main chain local conformation relative to the other cathepsins, and in turn moves the corresponding interfering side chain (the smaller Thr147 of cruzipain, and Phe141 of cathepsin...
- 11
- 385
- 0
ASSESSMENT OF EXPLOSIVE DESTRUCTION TECHNOLOGIES FOR SPECIFIC MUNITIONS AT THE BLUE GRASS AND PUEBLO CHEMICAL AGENT DESTRUCTION PILOT PLANTS ppt
Ngày tải lên :
22/03/2014, 10:20
... detonation of the munition’s explosive fill, if there is any If there is no explosive fill, the heat of the chamber will cause the agent to vaporize, rupturing the munition casing and exposing the agent ... to the agent; in the case of a mortar, its base is cut Mustard agent is drained from the weapons, and the agent cavity of each munition is washed with high-pressure water Agent is sent to the ... have been processed At the end of the processing campaign for each agent type, essentially all of the agent monitors have to be changed from the previous agent type to the new agent type Changing...
- 135
- 453
- 0
The Ubiquitous Triclosan A common antibacterial agent exposed ppt
Ngày tải lên :
23/03/2014, 10:20
... cause an eczematous rash, usually on the face, neck, diet may facilitate atopy by influencing the overall patthe back of the hands, and on the sun-exposed areas of the tern of commensals and pathogens…thus ... proposed use(s), and that the benefits of the drug outweigh the risks; the drug’s proposed labeling is appropriate; and the manufacturing methods used are able to maintain the drug’s quality, identity, ... every year For example, in Sweden to wash their hands or brush their teeth The dermal LD50 in 1998 alone, 25% of the total amount of toothpaste sold (the lethal dose that kills 50 percent of a...
- 6
- 78
- 0
Agent-based and analytical modeling to evaluate the effectiveness of greenbelts potx
Ngày tải lên :
23/03/2014, 13:20
... the greenbelt We assume that if a single agent lives right of the greenbelt it must cross the greenbelt to get services Once the agent crosses the greenbelt, which is width w, the agent has the ... from the greenbelt are unaffected by the greenbelt The aesthetic quality near the greenbelt is the maximum of (a) the score based on the predefined pattern and (b) the score based on proximity to the ... constraint on the mathematical model that g!M (Section 2.1) We then recorded whether or not any of the agents located to the right of the greenbelt To measure the degree to which the greenbelt...
- 13
- 402
- 0
Why (Special Agent) Johnny (Still) Can’t Encrypt: A Security Analysis of the APCO Project 25 Two-Way Radio System docx
Ngày tải lên :
23/03/2014, 13:20
... transmitterbythen sends silence for the voice At convert the LDU1 and LDU2 of the is done multiplying bits by encoding the See Figure for the the this end oftothe thatData encoding ofThe terminating ... users accidentally set their radios for clear mode, the other users will still hear them And as long as the (mistakenly) clear users have the correct keys, they will still hear their cohorts’ encrypted ... in the metadata LDU1 and LDU2 the other voice data units The detailed structure of the data units is given in Section by they carry), followed a terminator frame See Fig- Jp*\ ure Note that the...
- 16
- 1.2K
- 0