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Báo cáo y học: "Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats" potx
... diseases Although it is premature to suggest that the nine genes are candidate genes for these QTLs, the data indicate that gene expression profiling will be useful to assist in cloning of genes from ... The differential mRNA expression of some genes of interest was compared with the expression of the encoded protein using either FACS or ELISA The statistic significance threshold values for differential ... toward being differentially expressed To further investigate the possibility that these genes play a vital role in arthritis pathogenesis, their genomic locations were compared with those of...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 – the human ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit ... Hodgkin’s disease; capn4: the calpain subunit gene; ND4: the NADH dehydrogenase subunit gene; IL5: the interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
... controlling mRNA stability are important factors determining their zonal expression in liver In addition, we found that several other genes with unknown localization in the liver show distinct expression ... differences in expression of genes encoding enzymes and other proteins involved in intermediary metabolism Perivenous expression is indicated by green, and genes with preferential periportal expression ... in accordance with the known perivenous activity of this enzyme [11,12] The only gene of the glycolytic pathway that is mainly expressed in periportal hepatocytes is that encoding pyruvate kinase...
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báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues" docx
... within nectary-enriched genes The large numbers of genes displaying nectary-enriched expression profiles suggests common mechanisms for restricting and/or activating their expression within these ... Refiner module The probe signal levels were quantile-normalized and then summarized using the RMA algorithm [69] Gene expression values were further linearly scaled up to a media of 100 in the Expressionist® ... motifs within nectary-specific genes suggests that they may provide a basis for regulating nectary-specific gene expression Finally, it should be noted that multiple genes involved in aspects...
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báo cáo khoa học: " Differential gene expression in an elite hybrid rice cultivar (Oryza sativa, L) and its parental lines based on SAGE data" doc
... leaves (101 genes, Table 4), are mostly over-dominant Finally, in the process of summarizing gene distributions in the twelve patterns, we found that a minority of the differentially-expressed genes ... (9311_Chr12_1 510) genes were found highly expressed in the hybrid as compared to its parental lines despite the fact that the expression of these genes are already higher in its paternal line 93-11 than in ... 275 Plastocyanin-like 6017 Major intrinsic protein 263 EPSP synthase (3-phosphoshikimate 1carboxyvinyltransferase) 2097 Thiamine biosynthesis Thi4 protein 410 Ubiquitin 263 Protein of unknown...
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Báo cáo y học: "Differential gene expression in anatomical compartments of the human eye" pptx
... cells that have lost their organelles, including nuclei Genes encoding the subunits of crystallins, the predominant structural proteins in the lens, were prominent in the lens signature, including ... signature was the enrichment of genes encoding proteins involved in endocytosis, including clathrin (CLTCL1, PICALM) and caveolin (CAV1) Currently, intercellular transport within the lens is thought ... 85% of the retinal signature from Figures to be included in the retina set, while less than 5% of these genes were contained in any of the other compartment sets Genetic diseases affecting the lens,...
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Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx
... non-linear [49] Consequently, Tb.Sp is non-linearly associated with OCN/GAPDH mRNA gene expression The data in this study also indicated that with increasing ALP gene expression there is decreasing ... joint cartilage degeneration supports the concept of generalised involvement of bone in the pathogenesis of OA 10 11 12 13 14 Competing interests The authors declare that they have no competing ... higher than IGF-I mRNA in OA, this study indicated similar positive associations between the two IGFs in both OA and non-OA individuals, indicating that the probable co -expression of the two...
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Báo cáo y học: "Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients" pptx
... better type I/type II collagen ratio in OA chondrocytes than in the OA cells Combined with the slightly lower ALK-1 expression in the OA cells, this suggests that the phenotypic state of OA chondrocytes ... manufacturing engineering practice guidelines required for tissue Gene expression analysis RNA was extracted and isolated from chondrocytes using the RNeasy mini kit according to the manufacturer's instructions ... sponge on the other side The sponge side of the scaffold was seeded with × 106 or × 106 OA chondrocytes/cm2 or × 106 healthy chondrocytes/cm2 The cell-scaffold constructs were then cultured in vitro...
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Báo cáo y học: "Differential gene expression patterns in cyclooxygenase-1 and cyclooxygenase-2 deficient mouse brain" ppsx
... exhibited increased expression in both genotypes The expression of the other three genes (mitochondrial inner membrane protein, GABA transporter [GAT]3, and ring finger protein 24) changed in opposite ... either in the same or in the opposite direction In the cerebral cortex, there were four genes with altered expression in both COX-1-/- and COX-2-/- mice Of these four, Rho-GDP dissociation inhibitor ... previous study examining the incorporation of AA in the brains of COX-2-/- mice [17] suggested that the increase in baseline incorporation of AA in awake COX-2-/- mice is possibly due to increased β...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... the pHPMA polymer); the ordinates represent the linear ratio of differential gene expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene ... impact on the gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and in vivo) Microarray Study Differential Gene Expression Differential ... repressed expression of the ZCD1 gene C) a repressed expression of BSG gene It is coincidence that the detected genes, the PKN1 and BSG gene cluster on the adjacent chromosomal location: The gene expression...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... decrease the IL-1b-induced binding of these factors In addition, the expression of genes, associated with atherosclerosis, that contain overlapping GC-box expression are controlled at the transcriptional ... blotting) the concentrations of the caveolin-1 and caveolin-3 proteins in the caveolae of cells stimulated with IL-1b in order to determine whether caveolin synthesis is also modulated by PUFA incorporation ... mediated mainly via the Ras/Raf pathway, and that incorporation of n-3 PUFAs interferes with these signalling molecules The cyclin D1 promoter activity induced by Ras or Raf dominant-positive expression...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... decreased in all defined adipose depots in response to PM2.5 exposure The levels of down-regulation of both these genes were pronounced in iBAT and mBAT in comparison with the WAT depots The gene expression ... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... antibodies After the PBS rinsing, the stain was developed using Fast 3, 3’-diaminobenzidine tablet sets (D4293; Sigma, St Louis, MO) The sections were then counterstained with hematoxylin and analyzed...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... further elucidate the insulin-to-PDK pathway downstream of PtdIns3K In contrast to the results obtained with the PtdIns3K inhibitors, rapamycin (100 nm) did not reverse the effects of insulin ... selectively inhibited by the mitogen-activated protein kinase kinase (MEK) inhibitor, U0126 [32,33] Therefore, the role of the MAPK pathway in regulating PDK mRNA expression was investigated by incubating ... to examine the role of PtdIns3K in regulating PDK transcript abundance in response to insulin (Fig 2) Incubation with either LY294002 (50 lm) or wortmannin (100 nm) significantly inhibited the effects...
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Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt
... for protein folding, are included in the UPR gene list Among them, BiP (three genes), calnexin (two genes), calreticulin (two genes), and AtHSP 907 (one gene) have been shown to be induced by ... than 1.2-fold with all the treatments with TM, dithiothreitol and AZC In addition, from the list, we eliminated the genes in which the degree of variation was lower than the sum of the background ... the luminal domain of At SEL-1L was eluted with the urea-binding buffer containing m imidazole, and then concentrated with a Centriprep -10 (Millipore, Billerica, MA) The purified luminal domain...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... Western blotting analysis The extent of protein expression is much lower in the HEK293 cells treated with Rz-1 ( 5%) and with Rz-2 ( 10% ) plasmids relative to the amount of BACE in the control ... Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein using our engineered ribozymes In order to check the potential of plasmid ... plasmids Ribozyme encoding plasmids were transiently transfected into the tested cells with the aid of lipofectin Expression of the ribozymes was observed in the course of time The nucleic acid fraction,...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... obtain higher energy yields; this in turn changes the quinol/quinone ratio even more dramatically than anaerobic growth with glycerol in the absence of nitrate It appears that this quinol/quinone ... was inserted and the resulting interrupted genes were cloned into the suicide vector, pKAS46 These constructs were then introduced into the chromosome of RAS46 by side-specific recombination The ... of the anaerobic respiratory chain As the most hydrophobic regions of NifL-protein are located in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal...
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Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
... significant increase in the expression of the cyclin-dependent kinase inhibitor p21, a target of p53 [10] Comprehensive gene expression profiling has revealed that the pattern of gene expression in CCA ... intron of the ANX4 gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in the ... that found in the other EOC subtypes [29,30] The data obtained in the present study together with those of these previous reports suggest that p53 functional status is critical in governing the...
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Báo cáo khoa học: Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes doc
... coverage of gene expression profile by cDNA -differential display seems still incomplete, given the lack of a few genes in the entire biosynthetic pathway of anthocyanins in the collected gene repertoire ... [20], indicating again the universal necessity of GST-like proteins in anthocyanin accumulation and the interspecies functional compatibility of these proteins To investigate whether PfGST1 can in uence ... together with AN9 and TT19, but distinct from the maize Bz2 protein [12], which plays a similar role in uptake of anthocyanin into vacuoles This presumably reflects the difference in the origin...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
... right margin designate domains The DNA binding domain (C region) is underlined and the ligand binding domain (E region) is underlined with dashes The helix–turn–zipper motif is double-underlined and ... surprising result was that the observed declines in the level of PIEcR-B1 mRNA did not occur in the presence of ANS, suggesting that a 20E-induced protein(s) synthesis was involved in these decreases ... subsequent decline This result agrees with the effects of anisomycin on the induction of MsEcR-B1 mRNA by 20E Therefore, during the differentiation of imaginal wing cells, the expression of PIEcR-B1...
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Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx
... cytokinins and hormones The main purpose of a regulated gene expression system is to control the timing and levels of transgene expression in vivo Whether the expressed protein remains within the ... of more than 100 in the dimethylsulfoxide-treated 293 cells In our experience, the genes that show signal detection values less than 100 are not reliable indicators of gene expression; therefore, ... 100 Out of these three genes, we selected tousled-like kinase (Tlk) gene for qRT-PCR analysis The data from both methods showed that the Tlk gene was induced by Pon A by two-fold (Fig 5B,C) In...
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