telomere as an important player in regulation of microbial pathogen virulence

... rabbit anti-human Fibβ, rabbit anti-human Fibγ and rabbit anti-human fibronectin Anti-rabbit HRP labelled antibody was used as a secondary antibody ECL detection was carried out by means of Supersignal ... was checked by means of Ponceau S staining Detection of citrullinated proteins The presence of citrullinated proteins on the nitrocellulose blots was detected using the anti-modified citrulline ... serum of RA patients Indeed, recently, it has been shown that fibrinogen and citrullinecontaining fibrinogen are present in the IC of RA patients [15] Because of the pathogenic nature of IC in RA,...

Ngày tải lên: 12/08/2014, 14:22

... amino group of the main chain at Glu268 The oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds The interactions should maintain ... effective in increasing resistance of ALP I to anionic surfactants The unfolding caused by surfactants occurred in a moderate manner in comparison with denaturation by high alkalinity, and the ... Wild-type Sendai was stable at pH 12 and it maintained 80% of the original activity after h The activity of N263D-Sendai decreased to 45% and 10% of the original after and h of incubation at pH...

Ngày tải lên: 21/02/2014, 03:20

... by imaging studies as in these two cases MRI is superior to a CT scan in such Figure MRI of the brain of the patient in case showing a single cystic enhanced lesion Singhal et al Journal of Medical ... +1 urine albumin Bearing this in the mind, an emergency MRI of the head was done which detected the spherical ring-enhancing lesion The diagnosis of neurocysticercosis was made on the basis of ... albuminuria; no past history of any seizures; and both women presented in the second trimester Marginally raised blood pressure and +1 urine albumin may be present in acute stages of convulsions In...

Ngày tải lên: 11/08/2014, 00:23

... change of the concentration of MHC in human serum and plasma is an important factor to examine the muscle injury and myosin-related diseases 12 1.3 The role of Outer membrane protein A (OmpA) in ... loops are involved in recognition of different phage protein as well as involving in conjugation and in binding of a phage and a bacteriocin (Morona et al, 1984) 1.3.3 The role of OmpA in virulence ... of outer membrane proteins in host -pathogen interaction and vaccine development 1.2 1.3 Myosin heavy chain (MHC) and its Clinical significance 1.2.1 Overall review of myosin and MHC 1.2.2 Clinical...

Ngày tải lên: 03/10/2015, 20:32

... a-L-1,3-Arabinofuranosidase activity in the GALA preparation was measured as described before [27], using 10 and 100 lL of the enzyme preparation (in duplicate) and incubation times of and h Analytical ... was pregrown as described in Material and methods, and transferred for h to minimal medium containing carbon sources as indicated in Fig Expression of galA was only detected in the presence of ... hybridizing bands NW290 was transformed with pIM3982 and transformants were analyzed for b-1,4-endogalactanase production using azo-galactan Transformant NW290::pIM3982.77 was selected as the...

Ngày tải lên: 21/02/2014, 01:21

... Zeynalzadegan A, Qi X, Jin H, Kim CU, Swaminathan S et al (2008) Modeling, analysis, and validation of a novel HIV integrase structure provide insights into the binding modes of potent integrase inhibitors ... below and consist of protonation and a concomitant swing of the Asp64 carboxylate group out of its metal-coordinating position, and into a dualhydrogen-bond lock with a neighboring asparagine In ... physiological cofactor of the NTD The most instructive case is Cd2+ coordination by IN One has to clearly distinguish the cases of HIV-1 IN and ASV IN, because the above-mentioned Cys65 of HIV-1 IN actually...

Ngày tải lên: 29/03/2014, 23:20

... function as a transcription factor The transactivation ability of MtCBF4 was analyzed using a yeast assay system The GAL4 DNA-binding domain-MtCBF4 recombinant plasmid was transformed into yeast cells ... underlined) An NcoI-SpeI fragment containing MtCBF4 cDNA was inserted into pCAMBIA1302 containing the 35S CaMV promoter and a hygromycin (kanamycin) resistance marker The plasmid was introduced into ... an increased rate of anthocyanin biosynthesis improves a plant’s ability to guard against oxidative damage and protects it from such injury Anthocyanin reportedly functions in the cell membrane...

Ngày tải lên: 11/08/2014, 11:21

... initial to 12 hours of the 24 hour intravenous infusions [26] An older, underpowered RCT showed no difference in outcomes between omeprazole and a combination of somatostatin and ranitidine in ... measured by laser Doppler flowmetry in rabbits and man Am J Gastroenterol 1999, 94:1077-1082 19 Collins R, Langman M: Treatment with histamine H2 antagonists in acute upper gastrointestinal hemorrhage ... Somatostatin and ranitidine in the treatment of non-variceal upper gastrointestinal bleeding: a prospective, randomized, double-blind, controlled study Hepatogastroenterology 2000, 47:1325-1327 25 van...

Ngày tải lên: 12/08/2014, 23:24

... host-protective in containing inflammation to the site of infection [7] On the other hand, bronchoalveolar procoagulant activity can be disadvantageous if there is an excess of fibrin formation (one of the ... due to oxidation of thrombomodulin and shedding of thrombomodulin from the cell surface, and is associated with worse clinical outcomes The important role of activated protein S is further illustrated ... significantly diminished pulmonary inflammation in a murine model of intranasal lipopolysaccharide-induced ALI/ARDS [14,15] In conclusion, bronchoalveolar inflammation and coagulation activation are independent...

Ngày tải lên: 13/08/2014, 10:20

... was 10 mg·L-1 and 12.6 mg·L-1, respectively In conclusion, P hydropiper may play an important role in sewage disinfection in CWs, but its living inhibiting pathogenic micro-organisms in the ecosystem ... Deba, Tran Dang Xuan, Masaaki Yasuda, et al (2008) Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn var Radiata, J of Food ... about Ailanthus altissma and Brucea javanica, and indicated that their extracts not only inhibited the infection of TMV, but also had the inhibition for the replication of TMV Tan Q W (2007) indicated...

Ngày tải lên: 05/09/2013, 09:38

... transcription activation domain; DBD, yeast GAL4 DNA-binding domain; T, terminator sequence tion can result in significant changes in ligand binding, transactivation activity, and specificity [35,36] Kumar ... kanamycin Resistant T1 plants surviving on kanamycin-containing medium were transferred to soil and then moved to a greenhouse for further analysis Tobacco plants were transformed by employing ... CfEcRVY mutant requires only nanomolar concentrations of ligand for transactivation of the luciferase reporter gene, both in transient expression studies using tobacco protoplasts, and in transgenic...

Ngày tải lên: 16/03/2014, 06:20

... including serine, cysteine, aspartic and metalloproteinase inhibitors [11] Serine proteinases appears to be the largest family of proteinases, and plant serine PIs have been classified into several ... stage of infection and affected the development and extension of R solani hyphae in leaves In certain plants, susceptibility to infection by R solani decreases with increasing age of the plant; ... necrosis was substantially smaller in sense plants than in antisense and control plants: days after infection, the diameter of the lesions was about 44 mm in the leaves of antisense plants, but...

Ngày tải lên: 23/03/2014, 03:20

... using mouse anti-GFP as the first antibody and goat anti-mouse Ig linked to peroxidase as the second antibody Lipid analysis of whole cell extracts Cells were homogenized for under CO2 cooling in ... separating proteins using a 12.5% SDS/PAGE and transferring to nitrocellulose filters yEGFP tagged proteins were detected using mouse anti-GFP as the first antibody and goat anti-mouse Ig linked ... formation, indicating that Sec14p and the two homologues act as inhibitors of Plb1p in vivo This observation explains at least in part the low Plb1p activity in wild-type cells Interestingly, the inhibitory...

Ngày tải lên: 23/03/2014, 18:20

... in the p7.5:TetOA12L:NEO plasmid was used as a transient selective marker in the presence of Geneticin G418 sulfate (Invitrogen) The second recombination of NEOR-containing viruses occurred in ... resulting in the construction of the p7.5:TetOA12L:NEO plasmid Transfection of the p7.5:TetOA12L:NEO plasmid in concert with VV WR infection induced the first recombination The Neomycin resistance ... promoter (pA12L) and AG/A mutant plasmid of A12L (AG/A), and infected with vvtetOA12L in the absence of Tet The transient expression of A12L induced regular IV and IMV particles (panel a) while...

Ngày tải lên: 18/06/2014, 18:20

... Heidelberg, Germany) For the identification of allergens, proteins were transferred by semi-dry blotting and immunostaining as stated above For protein staining, blotting was performed onto polyvinylidene ... dehisces and releases pollen Maize pollen is anemophilous (dispersed by wind) and most pollen grains fall within a few meters of the tassel because of its high settling velocity In the investigated ... Parietaria, Platanus, pollen of early-, mid- and late-blooming trees, grass pollen mixtures, maize kernel, rye, nettle, goosefoot, rape, plantain, animal dander (dog and cat) and latex), as well as a commercially...

Ngày tải lên: 20/06/2014, 00:20

... in the p7.5:TetOA12L:NEO plasmid was used as a transient selective marker in the presence of Geneticin G418 sulfate (Invitrogen) The second recombination of NEOR-containing viruses occurred in ... resulting in the construction of the p7.5:TetOA12L:NEO plasmid Transfection of the p7.5:TetOA12L:NEO plasmid in concert with VV WR infection induced the first recombination The Neomycin resistance ... promoter (pA12L) and AG/A mutant plasmid of A12L (AG/A), and infected with vvtetOA12L in the absence of Tet The transient expression of A12L induced regular IV and IMV particles (panel a) while...

Ngày tải lên: 20/06/2014, 01:20

... the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related ... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated...

Ngày tải lên: 20/06/2014, 04:20

... the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related ... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated...

Ngày tải lên: 20/06/2014, 07:20

... selection of the right vaccine and improving the efficacy of vaccination 5.3 Capacity Building Training and education of field veterinarians in disease prevention, disease investigation and sample ... Regional Animal Health Centre in Ho Chi Minh City An important part of the laboratory training was Quality Assurance in the laboratory to ensure tests will be run according to a standard protocol and ... were trained in ELISA techniques for the detection of FMD antigen and antibody and the standardisation of reagents Training was carried out under a quality system emphasizing the importance of Quality...

Ngày tải lên: 22/06/2014, 12:20

... round of the project RAHO - now has an epidemiological unit and this project has assisted in developing the skills of the epidemiologists in serosurveillance, outbreak control and investigation of ... provision of resources and training of staff in required methods and quality assurance To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine ... strategies Completing these objectives will improve the diagnostic capability of the veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control This...

Ngày tải lên: 22/06/2014, 12:20