tams cancer cells and the tumour microenvironment

... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...

Ngày tải lên: 18/06/2014, 19:20

... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...

Ngày tải lên: 20/06/2014, 03:20

... reduce the number of viable PC3 cells by approximately 50% whereas 50 µM ErPC3 were needed to affect the viability of DU145 (E) and PC3 cells (F) PC3 cells and the less ErPC3-sensitive LNCaP cells ... reduced the number of viable LNCaP, PC3 and DU145 cells as determined by the WST-1 test In PC3 and DU145 cells the antineoplastic effects of the combination treatment could mainly be attributed to the ... Apoptosis-induction by ErPC3 and ionizing radiation in prostate cancer cell lines The WST-1 assay mirrors just the number of viable cells at a specific time point, but does not indicate whether the therapy effects...

Ngày tải lên: 09/08/2014, 09:20

... Medicine, NUS Their encouragement and guidance and their critique has been of paramount importance in directing the course of the work leading to this dissertation and all the knowledge and skills ... ligand (FasL), tumour necrosis factor alpha (TNFα) and TNF related apoptosis inducing ligand (TRAIL ) where these ligands bind to specific death domain receptors and induce the formation of the ... by the ANT in the inner mitochondrial membrane and the VDAC in the outer mitochondrial membrane and cyclophilin D in the matrix (Green and Kroemer, 2004; Zamzami and Kroemer, 2001) And a sudden...

Ngày tải lên: 14/09/2015, 14:04

... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... stem cells In the first, stem cells themselves are implanted in a diseased or injured organ in the hope that they will give rise to the mature cells needed by that organ In the second, the stem cells ... millimeter) The outer layer of the blasotocyst is called the trophoblast, and the cluster of cells inside the sphere is called the inner cell mass At this stage, there are about 70 trophoblast cells and...

Ngày tải lên: 15/03/2014, 09:20

... human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germs ... A., et al (1998); Embryonic stem cells lines derived from human blastocysts Science 282, 1145 - 1147 12 Raisman, G., (2001); Olfactory ensheathing cells - another miracle cure for spinal cord ... - 822 Jones, G M., et al (2000); Human embryonic stem cells technology Semin Reprod Med 18, 219 - 223 Orlic D et al (2001); Bone marrow cells regenerate infarcted myocardium nature 410, 701 -...

Ngày tải lên: 02/04/2014, 22:20

... in metastatic cancer, and is less well known in primary cancer than the other genes The prognosis of metastatic cancer when the primary cancer is not known is worse than for other cancers; thus ... NIH3T3 and ras-NIH3T3 cells Wound times the concentration of ascorbic acids and the Wound healing assay on NIH3T3 and ras-NIH3T3 cells depending on the concentration of ascorbic acids and the treated ... JP, JY, SP and JY collected and analyzed the wounding healing experiments CY, KL and SL conceived and designed the experiments and analyzed the data The manuscript was written by CY, KL and SL All...

Ngày tải lên: 18/06/2014, 15:20

... to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) , medium (M -cells) and high (SM -cells) bioenergetic signature Cells were then treated with the following ... by ANOVA and post hoc Dunnett’s test Plots in (D) illustrate the inverse (3BrP and IA) and direct (5-FU) correlation that exists between the bioenergetic signature of the cells and the death-response ... The Bioenergetic Signature correlates with the cell-death response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) (A), medium (M -cells) (B) and...

Ngày tải lên: 18/06/2014, 19:20

... to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) , medium (M -cells) and high (SM -cells) bioenergetic signature Cells were then treated with the following ... by ANOVA and post hoc Dunnett’s test Plots in (D) illustrate the inverse (3BrP and IA) and direct (5-FU) correlation that exists between the bioenergetic signature of the cells and the death-response ... The Bioenergetic Signature correlates with the cell-death response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) (A), medium (M -cells) (B) and...

Ngày tải lên: 20/06/2014, 03:20

... analysis DM and MMY drafted the manuscript together VS performed and analyzed the β-catenin assays and participated in manuscript preparation SCC and MJ participated in the inception of the idea, ... advances the understanding regarding the molecular mechanisms involved in curcumin mediated chemo/radio-sensitization in ovarian cancer cells Materials and methods Cell culture and drugs A2780 and ... opsonization), clearance by the reticuloendothelial system, and non specific accumulation in organs [49] To counter these difficulties and to extend the circulation time of nanoparticles in the blood, nanoparticles...

Ngày tải lên: 20/06/2014, 07:20

... Unfractionated CD4+ cells (which contain CD5high-expressing cells) prevented the induction of autoimmunity when transferred together with the CD5low cells, implying that the TR cells were contained ... still remain between the surface phenotype of TR and primed T cells, but they are more relative than absolute For example, although both primed T cells and TR cells express CD25, the latter does so ... with immature dendritic cells) As yet it is unclear whether de facto Foxp3+ TR cells can be generated in the periphery or whether the Tr1 cells produced from conventional Th0 cells are equivalent...

Ngày tải lên: 09/08/2014, 01:23

... described Furthermore, it is suggested that cancer researchers should look not just at the cancer cells, but also at the environment in which they interact, with cancers eliciting the aid of fibroblasts, ... fibroblasts, endothelial cells and immune cells [33] Hypoxia has been demonstrated to induce the expression of both AT1b (AT1a and AT1b are subsets of AT1) and AT2 receptors in the rat carotid body and pancreas ... immunosuppression and further reducing inflammation with the use of NSAIDs will both inhibit tumour progression and allow currently developed immunotherapies, such as cancer vaccines, to promote their therapeutic...

Ngày tải lên: 11/08/2014, 08:21

... cages The animals were fed a standard diet with food and water ad libitum All in vivo studies complied with national legislation and were approved by the local authorities on the care and use ... (1:200) and CD4-APC (1:100) or their respective isotype control antibodies Cells were washed and consecutively fixed and permeabilized using cytofix/cytoperm solution (BD Biosciences) Thereafter, cells ... IL-6 and the acute phase gene Saa1 To study the effects of TNFR1 silencing in spleen, we performed FACS and qPCR analyzes on the splenocytes (Figure 6b, c) and cytokine measurements on the APC...

Ngày tải lên: 12/08/2014, 12:20

... tumor cells Endothelial-tumor cell interactions induce nitric oxide release from the hepatic endothelium Direct and indirect evidence from the literature has supported the hypothesis that the hepatic ... binding and stimulation of angiogenesis, and on the other hand, endothelial cells can contribute to host anti-metastatic responses by producing the cytotoxic molecule NO from arginine with the help ... produced by the vascular endothelial cells, to exert toxic effects on invading tumor cells, interact with endothelial adhesion molecules and regulate the subsequent metastatic tumor formation in the...

Ngày tải lên: 13/08/2014, 13:20

... mDCs and moDCs [12] and moDCs and KG-1 cells [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then ... molecules and migration to the lymph nodes During their maturation, DCs lose their antigen-capturing capacity and become mature immuno-stimulatory cells that have the ability to activate naïve T cells ... lower FGG and higher fascin) and surface marker expression (higher CD83), we then proceeded Despite the general similarities between mDCs and the two DC model systems, moDCs and KG-1 cells, our...

Ngày tải lên: 14/08/2014, 18:20

... hormone (FSH) The pituitary gland is a small gland located at the base of the brain, functionally linked to the hypothalamus It is divided into two lobes: the anterior or front lobe and the posterior ... between the hypothalamus and pituitary gland The anterior pituitary gland is functioning connected with the hypothalamus; nerve cells in the hypothalamus secrete neurohormones that act on the endocrine ... synthesized in and released from the hypothalamus, binds to GnRH receptors on the surface of the gonadotrope This leads to the synthesis and secretion of LH and FSH, which stimulate the production...

Ngày tải lên: 04/10/2015, 16:03

... tumor cells There was a non-statistically significant difference in the response of the cell lines to treatment with DHEA C33A and CASKI cells were more responsive to DHEA, and HeLa cells were the ... papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 Schwartz A & Pashko L (1995) Cancer prevention with dehydroepiandrosterone and non-androgenic ... of the patients; its local application was shown to be safe and well tolerated [20] Cervical cancer is the most common gynecological cancer in women between 25 and 55 years old, and it is the...

Ngày tải lên: 16/03/2014, 00:20

... colon cancer cells, we elected to focus on IxoA because of its abundance in the leaves and stems of the plant and its presence in the fruits To examine the mechanism that might account for the ... have a critical role in initiating the apoptotic program by antagonizing the function of the antiapoptotic BCL-2 and activating BAX and BAK [24] Therefore, the expression of BIM ⁄ BOD was evaluated ... that possess BH1, BH2 and BH3 regions and those that only possess the BH3 region [36,39] Pro-apoptotic proteins induce the release of cytochrome c from the mitochondria, and their ability to achieve...

Ngày tải lên: 23/03/2014, 10:20

... breast cancer cells To investigate the effects of hypoxia on the localization of BRCA1 in mammary epithelial cells, we subjected malignant (MDA-MB-468 and MCF-7) and nonmalignant (MCF-12A and HMEC) ... hereditary breast cancer cases, and its expression is reduced in sporadic cancers Therefore, the level of functional protein is decreased, and this may hinder the response of cells to TRAIL In ... fractionation of cells, followed by western blot analysis, to substantiate and quantify the changes between the nuclear and nonnuclear compartments in malignant cells Exposure of MDA-MB-468 cells to...

Ngày tải lên: 30/03/2014, 03:20

... overexpressed ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... cancer cells [8] and stabilizes the HER2 oncoprotein in pancreatic cancer cells [7] In the present study, we analyzed the expression of the HER2 protein in MUC4transfected ovarian cancer cells The ... MUC4-transfected ovarian cancer cells and these colonies were isolated from the cells and analyzed with cancer stem cell markers There was a slight change in the molecular weight of MUC4 protein in the isolated...

Ngày tải lên: 20/06/2014, 07:20