suppression of ldl oxidation by tea polyphenols

... pathway by perturbing the dynamics of microtubules in HeLa cells One of the major obstacles in the treatment of cancer is the toxicity of anticancer drugs Combination of two inducers of apoptosis ... cells was confirmed by the cleavage of poly (ADP) ribose polymerase and the fragmentation of DNA After 20 h treatment of HeLa cells with benomyl, the level of phosphorylation of bcl2 increased ... by using image-pro plus software (Media Cybernetics, Silver Spring, MD, USA) The effect of benomyl on chromosome congression was calculated by measuring the ratio of the width to the height of...

Ngày tải lên: 30/03/2014, 10:20

... regulation of aerobic methane oxidation In contrast, understanding of the process of anaerobic methane oxidation is in its infancy Geochemical evidence points strongly towards a coupling of anaerobic ... variant of F430, a cofactor used by the classical MCR, but the two cofactors differ in molecular weight as determined by mass spectrometry The genes encoding this protein were sequenced as a part of ... metabolism of single-carbon compounds in M capsulatus (Figure 2) has been greatly simplified by the addition of data available from pre-genomic analyses [7-9,21] and from the initial analysis of the...

Ngày tải lên: 14/08/2014, 14:21

... induction) of the IFN-b gene promoter by Cardif, and that this suppression was dependent on the serine protease activity of NS3-4A (Fig 6A) This result was supported by the results of the dimerization ... activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway We obtained further evidence by examining the status of the phosphorylation and dimerization of IRF-3 The results ... Limited suppression of the IFN system by HCV NS3-4A Relative level of TLR4 mRNA (%) B 100 100 75 50 25 GL2 TLR3 TLR4 TRIF 75 50 25 pIFN-β(-125)-Luc Relative luciferase activity Relative level of TLR3...

Ngày tải lên: 18/02/2014, 16:20

... A model of possible mechanism of suppression of IF1R69 mutant by 23S processing stem mutations (A) Effect of the mutant IF1R69L on subunit association in the cold The forward reaction of subunit ... a total absence of a fully mature 23S terminus; instead, a slightly truncated product was observed To rule out any possible involvement of Era in cold sensitivity suppression of mutant IF1, we ... set of suppressor mutations were found to be located in the 23S rRNA processing stem, and not in the structural part of the mature rRNA Our data suggest that the mechanism of suppression of cold...

Ngày tải lên: 06/03/2014, 00:21

... effect of either BetA, overexpression of CREB in the presence of BetA, or siCREB alone, further demonstrating that the BetA-induced SOD2 suppression is regulated by CREB BetA-mediated SOD2 suppression ... element, the effect of CREB protein on SOD2 reporter activity was examined The SOD2 WT reporter (SOD2 )1500) showed suppression by BetA treatment, overexpression of CREB in the presence of BetA totally ... (1995) Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis Nat Med 1, 1046– 1051 Kasperczyk H, La Ferla-Bruhl K, Westhoff MA, Behrend...

Ngày tải lên: 16/03/2014, 01:20

... accumulation by oxLDL or acLDL in HMDM and in J774 cells in both these conditions [25–27] In other studies, however, no effect on the induction of foam cell formation by acLDL or oxLDL in the presence of ... induction of lipid accumulation in the cells by the particles is enhanced when they are protected from oxidation, we investigated the mechanism of this effect by comparing the influence of CRLPs ... separated by TLC, as described previously [22] Proteins were harvested from the plates, after extraction of the lipids, by the addition of mL of NaOH (1 M) mRNA analysis The relative abundance of transcripts...

Ngày tải lên: 16/03/2014, 16:20

... indicated for the oxidation of N-hydroxyguanidines by one-electron oxidants and for the oxidation of benzamidoximes by H2O2 in the presence of HRP [15,37,38] In addition, the formation of intermediate ... after by the addition of catalase Analysis of the reaction mixtures by RP-HPLC using UV-detection revealed a signicant decrease of the amount of starting N-hydroxyguanidine and the formation of ... The oxidation of N-hydroxyguanidine and of NOHA by H2O2 catalysed by MP8 clearly involves an active species derived from the haem of MP8 as metal-free MP8 was inactive It also involves high oxidation...

Ngày tải lên: 17/03/2014, 09:20

... in an increase in the number of binding sites and a decrease of the affinity of the uPAR [38,39] The quantification of uPAR expression by measurement of the amount of uPA bound is thus not ideal ... 2002 uPA and uPAR possibly through suppression of upstream targets of ERK Thus, one of the functions of bikunin is the regulation of uPA synthesis and uPA binding by modulating uPAR It is most likely ... presence of 100 nM bikunin The maximal Ó FEBS 2002 Suppression of uPAR by bikunin (Eur J Biochem 269) 3949 Fig Effects of PMA and bikunin on functional uPAR protein expression in HRA cells measured by...

Ngày tải lên: 17/03/2014, 11:20

... conduct of animal research P sativum copper amine oxidase assay Oxidation of agmatine by P sativum copper amine oxidase was investigated spectrophotometrically by following the formation of a pink ... values of kcat and K were independent m of the enzymatic assay used (spectrophotometric vs polarographic) The stoichiometric analysis of the enzymatic oxidation of agmatine yields a molar ratio of ... corresponding to a flip angle of 90° was 7.4 ls The spin system of the agmatine oxidation product was assigned by COSY, by setting the flip angle of the second pulse to 35° To this purpose, 256 t1 increments...

Ngày tải lên: 17/03/2014, 23:20

... production Furthermore, inhibition of FXR1 production can abolish the suppression of TNF-a TGF inhibits LPS induction of TNF via FXR1 protein production induced by TGF-b1 FXR1 therefore plays an ... obtained by measurement of the TNF-a protein concentration by ELISA (Fig S1A–C) To determine whether TGF-b1-dependent inhibition of LPS-induced TNF-a protein production occurred at the level of transcription, ... expected, two different isoforms of FXR1 were visualized by western blotting with antibody against FXR1 following the addition of TGF-b1 and TGF-b1 ⁄ LPS [39,40] The pattern of FXR1 induction suggests...

Ngày tải lên: 22/03/2014, 21:21

... significantly changed by any of the four types of CRLPs Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, ... reach significance (Fig 3C) By contrast, CRLPs had no effect on the secretion of TGFb at any of the time points assessed (Fig 3E) The abundance of mRNA transcripts for each of the cytokines was determined ... isoforms of IjB exist, the most predominant is IjBa [15] Phosphorylation of IjB by upstream kinases results in its Lys48-linked polyubiquitylation and degradation, permitting translocation of...

Ngày tải lên: 23/03/2014, 04:20

... [15,16] Macrophage expression of adipophilin is upregulated by oxLDL [17], acetylated LDL (AcLDL) [18], enzymatically modified LDL [19] and by synthetic agonists of the peroxisome proliferator-activated ... promote LDL uptake by endocytosis [1] In addition, several studies have reported that macrophages can accumulate large amounts of cholesteryl ester (CE) through the uptake of oxidized LDL (oxLDL) by ... cells The data suggest that stimulation of fatty acid oxidation by AICAR is dominant over the inhibition of fatty acid oxidation by adipophilin The mechanism by which this occurs is unknown, but...

Ngày tải lên: 23/03/2014, 10:21

... The radical mechanism of oxidation of a nonphenolic substrate by a laccase->N–OH mediator system [22], it has time to follow an analogous H-abstraction route of oxidation of the nonphenolic substrate ... resulting in an increase of charge transport and therefore of current intensity Effect of the pK2 of the mediator and of solution pH on the laccase-catalyzed oxidation of 4-methoxybenzylalcohol ... 4-methoxybenzyl alcohol were run at a rate of mVÆs)1 Results and discussion Kinetics of the laccase-catalyzed oxidation of phenol red In a previous study, on the oxidation of oligomeric phenols, we unambiguously...

Ngày tải lên: 23/03/2014, 21:20

... clusterin inhibits the aggregation of a variety of different proteins that are partially denatured by reducing agents or heat-shock [19] Suppression of aggregation of these denatured proteins occurs ... least-squares boundary modeling by a superposition of sedimentation profiles of ideal nondiffusing particles [30] A regularization parameter of p ¼ 0.95, and a buffer density of 1.01 gÆmL)1 was used The ... specificity of the inhibition of amyloid formation by clusterin was assessed in control experiments using BSA The presence of BSA (100 lgÆmL)1) had negligible effect on the time-dependent formation of...

Ngày tải lên: 24/03/2014, 04:21

... the activation of HSF by NSAIDs may be in part caused by the inhibition of these enzymes, by which the cellular concentration of arachidonate increases IND induced thermotolerance of cells, whereas ... caused by an expanded polyQ tract is suppressed by overexpression of Hsp105a or Hsp70 in the cellular model [9,11], the suppression by NSAIDs seemed to be due to the accumulation of hsps by these ... caused by an expanded polyQ tract (data not shown) Ó FEBS 2004 Suppression of cytotoxicity by NSAIDs (Eur J Biochem 271) 4557 Fig Effects of SA on protein aggregation and cytotoxicity caused by an...

Ngày tải lên: 30/03/2014, 15:20

... value of total polyphenols and total catechins of tea products Total polyphenols (ppm GE) Tea types Minimum mean Maximum Oolong tea Black tea Green tea 12.37 11.39 5.56 Tea types Oolong tea 13.120.53 ... than those of black tea Table 3.3 Percentage of polyphenols and catechins in the first fusion versus total polyphenols and catechins content Tea types Polyphenols in first fusion Total polyphenols ... TCC of green tea was roughly half of TPC, yet still higher than oolong tea (40%) and black tea (14%) This results was appropriate for oolong and black tea, however indicated low quality of green...

Ngày tải lên: 22/07/2014, 06:20

... contribution of adenosine to the mechanism of action of methotrexate is well accepted, it is still unclear which adenosine receptors participate in the effect of methotrexate Results of early studies, ... Ludviksson BR, Valdimarsson H: The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules Clin Immunol 2005, ... comparable levels of the proinflammatory cytokine TNF-α in peritoneal exudates of wild-type mice and A2A knockout mice, but significantly increased accumulation of TNF-α in peritoneal exudates of A3 knockout...

Ngày tải lên: 09/08/2014, 07:20

... statins induce apoptosis by the activation of c-Jun N-terminal kinase 1/2 (JNK1/2) or by increasing the expression of Bim [6,7] However, several aspects of the mechanism by which statins induce ... apoptosis by activation of caspase-3 through inhibition of GGPP biosynthesis It has been reported that statins inhibit prenylation of small G proteins by suppressing the production of GGPP [4,8] ... caspase-3 through suppression of GGPP biosynthesis GGPP is an important membrane-anchoring molecule of Ras protein A shortage of GGPP facilitates dissociation of Ras from the inner surface of the membrane,...

Ngày tải lên: 10/08/2014, 10:21

... cultures caused suppression of NO production (Figure 2) The minimum concentration of CAM, which caused significant suppression of NO production was 0.4 μg/ml (Figure 2) The third set of experiments ... absence of the agents for 12 hours iNOS levels in cytosol were examined by ELISA As shown in Figure 5A, the addition of CAM at more that 0.4 μg/ml into cell cultures caused significant suppression of ... phosphorylation of MAPKs The sixth set of experiments was undertaken to examine the influence of CAM and M-4 on iNOS mRNA The final set of experiments was undertaken to examine the influence of CAM and...

Ngày tải lên: 11/08/2014, 03:20

... cultures caused suppression of NO production (Figure 2) The minimum concentration of CAM, which caused significant suppression of NO production was 0.4 μg/ml (Figure 2) The third set of experiments ... absence of the agents for 12 hours iNOS levels in cytosol were examined by ELISA As shown in Figure 5A, the addition of CAM at more that 0.4 μg/ml into cell cultures caused significant suppression of ... phosphorylation of MAPKs The sixth set of experiments was undertaken to examine the influence of CAM and M-4 on iNOS mRNA The final set of experiments was undertaken to examine the influence of CAM and...

Ngày tải lên: 11/08/2014, 06:22