subcellular localization of recql4 helicase

... nucleus and that the subcellular localization of claudin-1 may dictate the metastatic capacity of melanoma cells Our findings suggest that nuclear versus cytoplasmic expression of claudin-1 94 may ... non-phosphorylatable causes nuclear localization of claudin-1 C) Mutations mimicking constitutive activation of its phosphorylatable sites cause exclusively cytoplasmic localizationof claudin-1 http://www.medsci.org ... and localization, and how the localization of claudin-1 can affect melanoma cell motility Significance Tight junction proteins have long been thought to be solely responsible for the transport of...

Ngày tải lên: 03/11/2012, 11:17

... polypeptide of this family and, as a soluble protein, may be washed out from the nucleus during subcellular fractionation The localization of Sfh1p resembles the localization of PITP isoform a in ... only deletions of SFH3 and SFH4 caused significant changes of the lipid composition of plasma membrane and total cell extracts as reported earlier [11] Subcellular localization of the Sec14p homologues ... 2003 Ó FEBS 2003 nucleotides upstream of the STOP codon of the ORF of interest and on the 3¢ end, 65–70 nucleotides of the genomic region downstream of the ORF of interest Transformants were recovered...

Ngày tải lên: 23/03/2014, 18:20

... VRK2 isoforms have a different subcellular localization Substrate specificity of VRK2 isoforms and p53 phosphorylation To identify and confirm the subcellular localization of both VRK2 isoforms ... Differential stabilization of p53 by VRK2 isoforms Fig Expression of two VRK2 isoforms (A) Detection of VRK2 proteins in several cell lines that were determined by immunoblotting of whole-cell extracts ... VRK1 this nuclear isoform might regulate p53 mainly by phosphorylation Results Localization of the endogenous human VRK2 protein First we determined the subcellular localization of the endogenous...

Ngày tải lên: 30/03/2014, 11:20

... phosphorylation of TauT or a putative regulator of TauT could be involved in the H2O2-induced reduction in the active taurine uptake in NIH3T3 cells Expression and subcellular localization of TauT The ... order to confirm changes in subcellular level of TauT expression and TauT localization upon supplementation of taurine and sucrose, we performed Western blotting analysis of TauT expression in cytosolic, ... phosphorylated form of TauT [29] Whether the variance in the 4652 J W Voss et al (Eur J Biochem 271) Ó FEBS 2004 Fig Modulation of subcellular localization and expression of TauT (A) NIH3T3 cells,...

Ngày tải lên: 30/03/2014, 15:20

... FEBS 2001 level of fetal and adult liver rats The results show a new localization of BCATm in the nuclei of fetal hepatocytes and the presence of an active and inactive form of the BCATm in fetal ... Nuclear BCAT activity This new localization of the BCATm in liver nuclei raised the question of whether any of the enzyme activity was actually associated with the nuclei of liver cells To answer this ... appearance of the 41-kDa protein on immunoblots was always associated with the presence of the highly active form of BCATm These results suggest the existence of an Fig Expression of BCATm mRNA...

Ngày tải lên: 31/03/2014, 23:20

... on these viruses Subcellular localization prediction of viral proteins in host cells We used the PSLT human subcellular localization predictor [22] to predict the localization of cytomegalovirus ... the localization of human proteins and has been shown to accurately predict the localization of mammalian proteins in general and thus is likely an appropriate choice for the prediction of the localization ... protein subcellular localization predictors can be used to rapidly annotate specific viral proteomes with a first and reasonably accurate estimate of intracellular localization The subcellular localization...

Ngày tải lên: 20/06/2014, 01:20

... fluorescence micrographs of the distributions of lysosomes and QDs in RBL cells a The distribution of lysosomes (green), b the distribution of QDs (red), c the merged image of (a) and (b) in which ... fluorescence micrographs of the distributions of Golgi complexes and QDs in RBL cells a The distribution of Golgi complexes (green), b the distribution of QDs (red), c the merged image of (a) and (b) in ... understanding of the cellular delivery and subcellular distribution of QDs are of particular importance for cellular labeling with QDs, especially the labeling of subcellular compartments Although QDs...

Ngày tải lên: 22/06/2014, 00:20

... study the locations of the DEV pUL51 Intracellular localization and distribution of DEV pUL51 in DEV-infected cells A detailed analysis of the intracellular localization of DEV pUL51 was investigated ... required to reside in various cellular compartments of the host cell to fulfill their functions [13] Therefore, knowledge of the subcellular localization of viral proteins in a host cell or virus-infected ... reacted with the pre-immune serum (Fig 2B) Subcellular localization and distribution of DEV pUL51 in DEV-infected cells To identify the precise localization of DEV pUL51 in DEVinfected cells, TIEM...

Ngày tải lên: 12/08/2014, 04:22

... protein subcellular localization database Nucleic Acids Res 2006, 34(Database issue):D213-D217 reports This approach nevertheless takes us another step closer to the subcellular localization of the ... from the remainder of the cellular content In general, the greater the number of biochemical separation steps used, the higher the purity, but this comes at the expense of loss of valuable material ... different classes of endosomes and proteasomes Analysis of continuous sucrose gradients resulted in the identification of over 22,000 peptides, corresponding to 2,200 proteins, of which 1,400 were...

Ngày tải lên: 14/08/2014, 16:21

... at a molar ratio of 100 : in a final volume of 150 lL Reactions were stopped by addition of one volume of · Laemmli sample buffer, followed by boiling at 95 °C for The effect of ubiquitin on the ... specificity and enzymatic properties of USP7 As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters for the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin ... domain of USP7, and particularly a fragment including amino acids 70–205, is sufficient to achieve nuclear localization of the enzyme Based on our results, USP7 can be added to the list of deubiquitinating...

Ngày tải lên: 07/03/2014, 05:20

... consequence of the presence of unfolded or incorrectly folded human IRP1 This implies that formation of inclusion bodies competes with assembly of the [4Fe)4S] cluster in IRP1 In addition, degradation of ... Formation of aggregates is not a general feature of the heterologous production of human aconitases in yeast mitochondria, but a particular property of human IRP1 The active participation of mitochondrial ... accumulation of transition metals seemed to correlate with the formation of m-hIRP1 aggregates Of particular interest, iron was found in almost equal proportions (within 15%) in mitochondria of yeast...

Ngày tải lên: 07/03/2014, 10:20

... 2006 FEBS 3263 Analyses of novel Thermus competence proteins J Rumszauer et al Subcellular localization of PilMNOWQ and PilA4 To determine the subcellular localization of the competence proteins ... transformation of T thermophilus HB27 Furthermore, we present the first information on the subcellular localization of the PilMNOWQ and PilA4 competence proteins and on the effect of mutations in ... organization of the genes, potential polar effects of marker insertions on downstream-located genes of the pil cluster could not be excluded, and therefore the question of whether the products of pilM,...

Ngày tải lên: 07/03/2014, 12:20

... Manifestation of SAR is associated with the expression and activity of a set of SAR genes [17] including the previously described PR genes An earlier, more rapid or more abundant accumulation of these ... Isolation and characterization of Arabidopsis mutants has been a powerful approach to decipher the mechanism of SAR By screening a collection of T-DNA tagged Page of 16 Arabidopsis lines for mutants ... proteins in tobacco leaf epidermal cells Localization of DIR1Δ1-25-EYFP was identical to that of the EYFP control, such that fluorescence was observed in 60 of 60 cells at the cell periphery, in...

Ngày tải lên: 11/08/2014, 11:21

... http://www.biomedcentral.com/1471-2229/10/44 Page of 19 Figure Models of the plant PUM-HD bound to RNA (A, B) Ribbon (left) and stick (right) models of the PUM-HDs of AtPUM2 (A) and Os01g62650 (B) bound to the RNA bases of Box of the ... 15 of 19 regions of the transcript that lie outside of the PUM-HD binding site The recruitment of other factors might also enhance the RNA binding specificity of the Pum-HD The convex surface of ... the core nucleotides of Box of the NRE1 (UUGUAUAU) (C) Sequence alignment of residues in helix of repeats 1-8 that provide putative RNA contact sites on the concave surface of the PUM-HD (D) Schematic...

Ngày tải lên: 12/08/2014, 03:21

... subcellular distribution of RGK proteins 57 3.4 Modulation of subcellular localization of RGK proteins by CaM 63 3.5 Modulation of localization of RGK proteins by 14-3-3 in the absence of CaM binding 65 ... Association of RGK proteins with 14-3-3 dimers 3-3 Cytoplasmic relocalization of RGK proteins by 14-3-3 3-3A Regulation of localization of Kir/Gem by 14-3-3 3-3B Regulation of localization of Rad ... Regulation of localization of Rem1 by 14-3-3 3-3D Regulation of localization of Rem2 by 14-3-3 3-4 RGK proteins deficient in CaM binding localize to nucleus 3-5 Cytoplasmic relocalization of RGK...

Ngày tải lên: 14/09/2015, 18:28

... The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting proteins Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the ... this band was not apparent on western blots of lysates of Y394F tau (Fig 2A) The absence of this larger species could indicate that phosphorylation of tau by Fyn is reduced and therefore, in addition ... tau revealed decreased electrophoretic mobility of tau, with the appearance of an  68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig 3) In...

Ngày tải lên: 14/02/2014, 14:20

... of D 3.5 Calabi-Yau categories 3.6 Proof of Theorem 3.2 Localization with a generalized Frobenius character 4.1 Localization on (generalized) Springer fibers Splitting of the Azumaya algebra of ... sum of pdim B copies of F ⊗ Oν−ρ 979 LOCALIZATION IN CHARACTERISTIC P 6.2.7 Proof of Theorem 6.2.1 Let FM ∈ Db (CohBχ,ν (1) (T ∗ B (1) ×h∗ (1) h∗ )) be the image of M under the equivalence of ... independent of p, the calculation of the cohomology of Springer fibers (i.e., the stalks of the Springer sheaf), reduces to the calculation of stalks of intersection cohomology sheaves of irreducible...

Ngày tải lên: 16/02/2014, 06:20

... the results of real-time PCR analysis of the mRNA expression of PRiMA I and AChET (C) Samples of extracts from the lower leg muscles of rat (birth to adult stage) containing 40 lg of protein were ... the localization of PRiMA-linked G4 AChE, sections of tibialis muscle were triple stained for PRiMA, SV48 and AChR The staining of PRiMA was coincident with that of SV48, rather than with that of ... To investigate the contribution of the motor neuron to PRiMA-linked G4 AChE at NMJs, we analyzed the effect of denervation on the localization of PRiMA The NMJs of the denervated tibialis and...

Ngày tải lên: 18/02/2014, 08:20

... the maturation of virgin queens for flying and mating should include regulation of VgR expression Here, we investigate the temporal ovarian expression and subcellular localization of the VgR in ... the onset of egg-laying behavior To begin to dissect the molecular mechanisms of reproduction in ants, we investigated the fire ant VgR temporal subcellular localization in the ovaries of both virgin ... of the egg-laying queen within a mature colony than in virgin queens [12] The subcellular localization of SiVgR signal was similar in virgin and mated queens, i.e expressed in the cytoplasm of...

Ngày tải lên: 18/02/2014, 08:20

... was composed of lL of Moloney murine leukemia virus reverse transcriptase buffer; 0.5 lL of BSA (10 mgÆmL)1), lL of total RNA (1.24 lgÆlL)1), 2.5 lL of dNTP (4 mm total), 1.5 lL of Random Primer ... amplifications of RpCAbr and RpCAtr with 18S amplification The number of cycles on the y-axis is the difference between the number of cycles required to amplify each transcript and the number of cycles ... is composed of a central obturaculum, mainly made of extracellular matrix, supporting many branchial filaments at its periphery The branchial filaments are composed of a single layer of epidermal...

Ngày tải lên: 18/02/2014, 16:20