structure of sp a and sp d

... detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( tar elamef )DS( yelwaD-eugarpS ytnewT o slamina latnemirepxE sdohteM dna slairetaM ... fo esaerced yltnacifingis A ]82[ la te uK gnawk-eaS 291 sa demialc neeb evah yeht dna ,alludem lanerda eht edistuo sllec dna snagro enircodne fo yteirav egral ni rucco ot dnuof neeb evah sGC ]6[ ... enircodne fo yduts lacimehcotsihonummi nA H adamaY ,J adamaY ,K ihcugaT ,N ihsigamaY ,N arumatiK ,HR onotraheoS 92 84-93 ,801 ,9991 veD gniegA hceM munedoud namuh fo sllec enircodne dna gniegA M...

Ngày tải lên: 07/08/2014, 18:21

... profile and the associated variation in QA and PA levels [7], and other investigations have clearly demonstrated that changes in ACMSD activity are readily reflected by serum and tissue QA levels ... image was obtained by optimal superposition of the hACMSD:DHAP and PfACMSD:ligand-free structures Protein residues are colored in white for hACMSD and in green for PfACMSD, with the DHAP ligand in ... at the 1.2 r level (B) The DHAP binding site with crucial protein residues and solvent molecules engaged in ligand recognition and stabilization are drawn as balls -and- sticks and spheres, respectively...

Ngày tải lên: 18/02/2014, 06:20

... (dobserved–drandom coil) in ppm for HtA at pH 4.1 and 298 K The amino acid sequence and the elements of secondary structure are shown; b-strands are represented by arrows and the a- helix by a spiral ... hydrolyzed bond [16] E96 and H137 in a- sarcin and E58 and H92 in RNase T1 act as the base and acid groups, respectively Comparing the 3D structures, E66 and H113 in HtA are in similar positions ... spectra were acquired in H2O and D2 O Processing of the spectra was performed using the program TOPSPIN (Bruker) Analysis of the spectra, manual assignment of backbone and side-chain protons, and...

Ngày tải lên: 18/02/2014, 08:20

... 280 nm Aldolase (150 kDa), ovalbumin (45 kDa), chymotrypsinogen A (25 kDa), and cytochrome c (12.3 kDa) (Serva, Heidelberg, Germany) were used as molecular mass standards Absorption and CD spectroscopy ... plot of the data from (A) in the range 0–100 mM Ca2+ (C) Modified Scatchard plot of the data from (B) DA corresponds to the change in relative activity The PLDa2 activity was measured against ... fact that EDTA effects a marked stabilization (Table 1), however, indicates an additional destabilizing effect by calcium ions Destabilization of PLDa2 by calcium ions was also indicated by the...

Ngày tải lên: 19/02/2014, 02:20

... N-terminal amide and His2 Aspartic acids Asp27 and Asp30 show lower mean pKa values than their model pKa values This may be a general property of Asp residues, as it has been observed in another study ... interactions associated with secondary, tertiary, and quaternary structure packing and stability In addition, we discuss the role of charges in function of RIIa D/ D MATERIALS AND METHODS Sample preparation ... C-terminal face is a highly charged surface with symmetrically arranged charged residues The charged face of RIIa D/ D contains residues Asp27, Asp30, Glu34, Arg38, Arg40, Glu41, Arg43, and Arg44, of...

Ngày tải lên: 08/03/2014, 22:20

... of C maritima agg Charybdin was characterized by biochemical methods and its structure determined by X-ray crystallography The DNA sequence, and derived amino acid sequence, revealed significant ... various tryptic fragments, and Dr M Aivaliotis and C Karapidaki for their contributions during the isolation and characterization of the protein RG would like to thank M Providaki, A Deli and ... omitted The 87-amino-acid residue sequence deduced from a tryptic fragment was superimposed on the structures of the 12 RIPs, and a swiss model [23] was derived and used as part B of the search model...

Ngày tải lên: 16/03/2014, 14:20

... reaction was started by addition of FAM-AUAA-TAMRA (final concentration 50 nm) After a distinct time interval, lL of trypsin (0.5 mgÆmL)1) was added The reaction was finalized by addition of lL of ... same orientation in the crystal as monomeric RNase A On the other hand, the decreased activity of the RATEs as compared with RNase A ([16] and Table 1) indicate a negative influence of the tandemization ... 6-carboxyfluorescein-dArU(dA)2-6-carboxytetramethylrhodamine (FAM-AUAA-TAMRA) as substrate] and cytotoxic (IC50 values between 12.9 lm and 40.0 lm with mammalian K-562 cells) All three variants showed thermodynamic...

Ngày tải lên: 22/03/2014, 16:21

... America and used without further purification Commercial anhydrous solvents were used throughout and transferred under an argon atmosphere Additionally, DCM was dried by distillation over CaH2, and ... mL of DCM The combined organic layers were washed twice with mL of a saturated solution of sodium thiosulfate, water, and brine, dried over Na2SO4 and evaporated under vacuum The residue was ... a saturated solution of sodium thiosulfate, water, and brine, dried over Na2SO4 and evaporated under vacuum The residue was purified by silica gel column chromatography (9/1 CHCl3/acetic acid)...

Ngày tải lên: 10/09/2015, 15:48

... O 338 Appendix HO O 5-33 C14H16O3 232.3 OH FT-IR Data 339 AcO O O 5-32 C16H18O4 274.3 340 Appendix AcO O OAc 5-35 C18H20O5 316.3 FT-IR Data 341 AcO O 5-38 C18H20O6 332.3 OAc O 342 Appendix HO ... 598.8 O TES FT-IR Data 333 HO OH 3-57 C16H22O3Si 290.4 O TES 334 Appendix HO O 3-35 C16H22O3Si 290.4 O TES FT-IR Data 335 HO O 3-34 C10H7IO3 302.1 O I 336 Appendix FT-IR Data 337 HO O 5-28 C14H16O3 ... 320 Appendix FT-IR Data 321 OH O O HO O O O OH O OH O 2-1 C44H32O12 752.7 O OH O 5-2 C18H14O3 278.3 O 322 Appendix I OH 5-8 C8H7IO2 262.0 O FT-IR Data 323 I OTs 5-9 C15H13IO4S 416.2 O 324 Appendix...

Ngày tải lên: 10/09/2015, 15:49

... Low-Resolution-Mass Data 353 OH O 5-2 C18H14O3 278.3 O 354 Appendix Low-Resolution-Mass Data 355 O 5-4 C36H24O4 520.6 O O O O O 5-6 C36H24O4 520.6 O O 356 Appendix Low-Resolution-Mass Data I OH 5-8 ... Appendix Low-Resolution-Mass Data AcO O OAc 5-35 C18H20O5 316.3 375 AcO O 5-38 C18H20O6 332.3 OAc O 376 Appendix Low-Resolution-Mass Data HO O 5-40 C14H16O4 248.3 O OH 377 378 Appendix ... 302.1 O I 370 Appendix Low-Resolution-Mass Data 371 HO O 5-28 C14H16O3 232.3 O 372 Appendix Low-Resolution-Mass Data HO O 5-33 C14H16O3 232.3 OH 373 AcO O O 5-32 C16H18O4 274.3 374 Appendix Low-Resolution-Mass...

Ngày tải lên: 10/09/2015, 15:50

... O 410 Appendix O HO O 5-28 C14H16O3 232.1099 High-Resolution-Mass Data 411 O HO OH 5-33 C14H16O3 232.1099 412 Appendix O AcO O 5-32 C16H18O4 274.1205 High-Resolution-Mass Data 413 O AcO OAc 5-35 ... OH O 386 Appendix High-Resolution-Mass Data 387 O O O O O O HO O O HO O 4-38 C48H38O14 838.2262 O OH O 388 Appendix High-Resolution-Mass Data 389 OH O 5-2 C18H14O3 278.0943 O 390 Appendix High-Resolution-Mass ... High-Resolution-Mass Data 395 O O OTs OTs OBn 5-10 C43H36O9S2 760.1801 396 Appendix O O OH OH OBn 5-11 C29H24O5 452.1624 High-Resolution-Mass Data 397 O O O O OBn 5-12 C29H24O5 452.1624 398 Appendix O O...

Ngày tải lên: 10/09/2015, 15:50

... 2011 Medicinal Chemistry Programme (PhD student under the supervision of Dr Martin Lear) • Affinity-Guided Isolation, Structure Elucidation and Total Synthesis of Laetirobin A and Its Analogue ... Conducted and evaluated gravimetric measurements for the development of Respimat , a new-generation, propellant-free liquid drug aerosol delivery device for inhalation therapy ® German Armed Forces, ... to theta = 27.49° 99.1 % Absorption correction Sadabs, (Sheldrick 2001) Max and transmission 0.9766 and 0.9540 Refinement method Full-matrix least-squares on F2 Data / restraints / parameters...

Ngày tải lên: 10/09/2015, 15:50

... 41 She made me to pay for the damage I had done a made b to pay c for d had done > b 42 He warned me to make an eye on my luggage as that place was full of thieves a me b to make c on d full ... weak c to d this > a 51 Sun weather can always be depended on in southern countries a Sun b always c on d in > a 52 The man that wife and family are away seems very lonely a that b and c are ... They visited America about a thousand years ago, on the eleventh century AD a visited b thousand c ago d on > d 38 Ha Dong is the town where I am born and grew up a is b where c am d grew >...

Ngày tải lên: 05/11/2012, 09:18

... Origins of the Bank of Canada I • The Bank was created by the Bank of Canada Act in 1934 and started operations in 1935 • Initially the Bank was a private institution but was nationalized in 1938, ... Factors making Bank of Canada dependent Joint responsibility system (since 1967 when the Bank of Canada Act was amended) Minister of Finance can issue a directive to the Bank indicating the specific ... Central Banks European Central Bank: highly independent Bank of England: similar to Bank of Canada since 1997 Japan: increased formal independence since 1998 Trend to greater independence: New Zealand,...

Ngày tải lên: 05/01/2014, 16:57

... molecules and peptide atoms) and rj is the standard deviation of B factors The normalized B factors have a zero mean and unit variance All atoms that satisfy Bz ‡ are treated as outliers and discarded ... importin -a and the CLIC4 NLS peptide, P1AKKYRNP6 Backbone carbonyl oxygens and amide nitrogens are shown as red and blue spheres, respectively Nitrogen and oxygen side-chain atoms are shown as blue and ... per image The crystal to detector distance was set at 220 mm and the beam width was set to 200 lm · 200 lm Structure determination and refinement After datasets had been initially processed and integrated...

Ngày tải lên: 14/02/2014, 19:20

... between crotamine (A and B), AaHII (PDB accession no 1PTX) (C and D) and HBD3 (PDB accession no 1KJ6) (E and F) Positively and negatively charged regions are coloured in blue and red, respectively ... three human b-defensins (HBD1, HBD2, HBD3) are also displayed for comparison of secondary structures location and disulfide patterns (*) is used for identical residues (:) for conserved ones and (.) ... Secondary structure of AaHII (A) and Crt (B) together with an alignment of segments of AaHII (A) and Crt (B) based on secondary structure elements possesses a net charge of +10 with respect to AaHII...

Ngày tải lên: 20/02/2014, 11:20

... pentadecanoic acid was added to the lipid A as internal standard The position of the double linkage in the unsaturated acid was determined by GLC/MS of its pyrrolidide derivative [27]: differences of 12 atomic ... 3-hydroxydecanoic acid residues (two with amide linkages and one as the secondary acid) were located at C2 and C2¢ The same acyl-deficient and phosphate-deficient glucosaminobiose nonreducing end ... centrifugation and dissolved in chloroform To make the solution transparent, some drops of methanol were added The solution obtained was washed with deionized water three times and evaporated Lipid A...

Ngày tải lên: 07/03/2014, 15:20

... 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢ 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and ... and 14 (Table 3, Figs and 6B) The values obtained for interface surface area, interaction 172 type (two-thirds hydrophobic, but also hydrogen bonds and salt-bridges), gap volume index and planarity ... protease cleavage, and purification over Superdex 75 (lane 7, arrowhead, anticipated molecular mass of 31.8 kDa) (B) Side and (D) top views of the NlpI dimer Chains are coloured from N- (dark blue)...

Ngày tải lên: 07/03/2014, 16:20

... observed when ‡ lm of distamycin was added to the DNA in the absence of protein (Fig 5A, lane 4) At lower concentrations, partial occupancy of distamycin did not shift DNA (lanes and 3) The DNA mobility ... atoms (A2 ) ˚ Water molecules (A2 ) ˚ rmsd bond length (A) rmsd bond angles (°) Ramachandran plot favoured (%) Ramachandran plot accepted (%) Ramachandran plot outliers (%) PDB code 34198 0.196 ... helix a4 , and Asn72, Thr77 and Arg81, positioned on a5 Interestingly, substitution of Arg61 and Arg81 impair DNA-binding in PapB, indicating that these residues are critical for DNA-binding also...

Ngày tải lên: 15/03/2014, 23:20

... of the association rate constant of BR (k¢) and the quantum yield of BR (c) The data for the a and b subunits within liganded tetrameric HbA are shown in (A) and (B), respectively Conditions: ... liganded HbA we carried out a series of experiments at four NaCl concentrations of 0.1, 0.5, 1.0 and 2.0 m The rate constant of BR and the quantum yield of BR, in the presence of NaCl, gave a direct ... pH and NaCl on the bimolecular association rate constant of O2 rebinding and the 6110 quantum yield of BR for the a and b subunits within the liganded dimer and tetramer of hemoglobin were determined...

Ngày tải lên: 16/03/2014, 14:20