structure biosynthesis and gene regulation of collagens in cartilage and bone
Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... inspection of the region spanning )120 nucleotide to )40 nucleotide indicated many potential cis-acting elements, including Sp1, Ets, Stat3, Octamer [35] binding sites and AP1 sites (Fig 3) and ... and regulate gene transcription in diverse cells through interaction with a variety of partner DNA binding proteins, including Sp1 family members and Ets family members, as observed in the current ... (CCAAT/enhancer-binding protein-beta) are involved in the glutamate/nitric oxide/cyclic-guanosine 5¢-monophosphate-mediated repression of mediated repression of gonadotropin-releasing hormone gene expression...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 10:21
... most chemokine receptors were downregulated in SF PMN, supporting the notion of a differential modulation of chemokine receptors and their ligands during chronic inflammation These findings suggest ... contaminating eosinophils accounted for CCL18 production Notably, no eosinophils were detectable in preparations of SF PMN Cell culture For culturing PMN, RPMI 1640 containing L-glutamine and sodium ... unit (U) of Omniscript reverse transcriptase (Qiagen) in a final volume of 25 μl at 37°C for 60 minutes and at 93°C for minutes PCR was performed in a 25 μl reaction mixture containing μl of cDNA...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
Ngày tải lên :
09/08/2014, 11:20
... cytokines on neurotrophin and neuropeptide gene expression in non-degenerate and degenerate nucleus pulposus cells To examine the effect of disc-related proinflammatory cytokines on neurotrophin and ... burden of back pain in the UK Pain 2000, 84:95-103 Freemont AJ, Le Maitre C, Watkins A, Hoyland JA: Degeneration of intervertebral discs: current understanding of cellular and molecular events, and ... pulposus and the inhibitory effects of certain drugs Spine 2005, 30:1501-1504 23 Le Maitre CL, Freemont AJ, Hoyland JA: The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration...
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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
Ngày tải lên :
10/08/2014, 05:21
... Triflavin inhibits platelet aggregation through direct interference with fibrinogen binding to the αIIbβ3 integrin [10] There is now a multitude of evidence suggesting that the binding of fibrinogen ... prostaglandin E1 (PGE1) (0.5 μM) and heparin (6.4 IU/ml) The washed platelets were finally suspended in Tyrode's solution containing bovine serum albumin (BSA) (3.5 mg/ml) The final concentration of ... Simvastatin (30 and 50 μM) did not significantly affect FITC-triflavin binding to the αIIbβ3 integrin in platelet suspensions (c, 4.8 ± 0.1; d, 4.9 ± 0.1) (Fig 1C), indicating that the inhibitory...
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Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
Ngày tải lên :
14/08/2014, 14:21
... change in nucleosome occupancy in a typical yeast gene in different transcriptional states (a) When there is no transcription, repressor proteins bind to their DNA-binding sites and maintain a ... segregation of intergenic regions of the genome into the aqueous phase Nagy et al [13] proposed that this may be a result of different efficiency of chromatin cross-linking along the genome and that ... Disruption of chromatin structure during gene activity Cell 1979, 16:807-814 Weintraub H, Groudine M: Chromosomal subunits in active genes have an altered conformation Science 1976, 193:848856 Reinke...
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Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
Ngày tải lên :
11/09/2015, 10:18
... actin structure of stress fibers α-actinin has a single actin-binding domain in its sequence and they exhibit bundling activity by forming the dimeric protein The two actin-binding domains of ... proteins (Turunen et al., 1994) In addition to this domain, two more actin-binding domains capable of binding F-actin and G-actin have been identified in N-terminal and middle regions of ezrin ... C-terminal actin-binding domain The identity of amino acid sequence with ezrin in each domain is indicated As shown in Fig 1.4, the amino acid sequences of ERM N-terminal membrane-binding domain are...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
Ngày tải lên :
08/03/2014, 16:20
... using GST fusion protein containing the RAS-binding domain of RAF Precipitated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top) Proteins of ... using GST fusion protein containing the RAS-binding domain of RAF Precipitated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top) Proteins of ... Thombin, convulxin and TPO induce activation of RAS RAS activation was measured through the ability of its activated form (RAS–GTP) to bind to a GST fusion protein consisting of the RAS-binding...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... effector of the TOR pathway and animals carrying a mutation in this gene also live longer; and ife-2 mutants accumulate pools of amino acids that are known to induce protein biogenesis and inhibit ... Possible link between accumulation of amino-acid pools, mitochondrial function, and the insulin and TOR pathways in C. elegans longevity Black lines indicate the activating or repressive function of ... in amino acid levels, especially branched-chain amino acids Falk et al [9] showed that mutations affecting complexes I, II and III of the electron transport chain (ETC), including some that increase...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
Ngày tải lên :
10/08/2014, 05:21
... role for STC-1 in inducing both tumorigeneisis and angiogenesis in cancer is not well understood In our present study, we found that STC-1 can promoted angiogenesis in vivo and in vitro Moreover, ... Page of Figure Tumorigenesis and angiogenesis of BGC cells in nude mice (A) Western blotting analysis of the expression of STC-1 in BGC after stable transfection (B) Mean volumes of the tumor in ... tumor in the flank of nude mice (C) Immunohistochemical staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in...
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REGULATION OF CHEMICALS IN EUROPE – REACH
Ngày tải lên :
16/04/2015, 09:05
... environment Maintenance and enhancement of the competitiveness of the EU chemical industry Prevention of fragmentation of the internal market Increased transparency Integration with international ... human intake No implicit assumption about a ”safe” intake 37 INTERPRETATION OF MARGIN OF EXPOSURE • Inter-species and intra-species differences • 100 would be sufficient • Nature of the carcinogenic ... human intake • Estimation of human exposure • Calculation of an MOE 36 MARGIN OF EXPOSURE (MOE) Definition: The ratio between the defined point on the dose-response curve for the adverse effect and...
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Understanding the regulation of cytokinesis in fission yeast
Ngày tải lên :
11/09/2015, 09:06
... Assembly of the actomyosin ring in fission yeast 11 1.3.1.3 Signaling pathways invovled in cytokinesis 14 Temporal regulation of cytokinesis 19 1.3.2.1 Cell cycle regulation of cytokinesis in metazoans ... proteins participate in cross-linking and/ or stabilization of F-actin, and anchor the actomyosin ring to plasma membrane Together, they organize F-actin and myosin II into a dynamic contractile ring ... cortical band overlying the nucleus This broad band of proteins then condenses into a structure containing F-actin and its associated components: Cdc8p and Ain1p (Wu et al., 2003) The actomyosin ring...
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Gene regulation of snake venom prothrombin activators
Ngày tải lên :
11/09/2015, 10:03
... our understanding of gene regulation of snake venom prothrombin activators viii LIST OF FIGURES Chapter One Figure 1.1: Domain architecture of mammalian FXa, trocarin D and pseutarin C catalytic ... The birth -and- death model of evolution assumes that new genes are constantly being created by gene duplication, and while some of the duplication genes get incorporated and maintained in the genome, ... agonists and antagonists of platelet aggregation, and hemorrhagic proteins (Braud et al 2000; Chow and Kini 2001; Hutton and Warrell 1993; Kini 2004; Kini and Chow 2001; Kini and Evans 1990; Markland...
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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
Ngày tải lên :
14/09/2015, 14:18
... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... are formed but ring constriction fails The actomyosin ring disassembles and continuous rounds of nuclear division occur in the absence of cytokinesis resulting in formation of multinucleated cells ... family member, plays a role in myosin II independent cytokinesis in the budding yeast (Lippincott and Li 1998; Vallen et al 2000) In wild-type dividing cells, the actomyosin ring undergoes contraction...
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Báo cáo y học: "The determinants of change in tibial plateau bone area in osteoarthritic knees: a cohort study" doc
Ngày tải lên :
09/08/2014, 06:22
... tibial bone area, and grade of medial joint-space narrowing explained 4.6% of variance in annual percentage change in medial tibial bone area BMI and grade of medial joint-space narrowing were independently ... osteophytes; grade of JSN=percentage change per increase in grade of jointspace narrowing; baseline tibial area = percentage change per mm2 increase in baseline tibial plateau bone area bIncludes age, ... grade of osteophytes, grade of joint-space narrowing, and baseline tibial plateau bone area (Table 3) In these analyses, BMI explained 7.3% of the variance in annual percentage change in medial...
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THE ROLE OF STAT3 IN OSTEOCLAST MEDIATED BONE RESORPTION
Ngày tải lên :
24/08/2014, 12:28
... plotted against the corresponding values of the criterion function At each point in the plot, a line is fitted to all of the points on each side of the current point The optimal number of clusters ... of remaining clusters is plotted against the minimum pairwise Bhattacharya distance Two lines are fitted to the points on either side of a testing point to find which partition results in the ... meaning of the abundance variables aj A cell’s fluorescent emission is the summation of the emissions of all dyes present in the cell and autofluorescence Since the information we are unmixing...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
Ngày tải lên :
16/03/2014, 18:20
... expression of CB1 in isolated cortical neurons was affected in both strains of R6 transgenic HD mice It appeared that the amino terminus of mutant huntingtin containing an expanded polyglutamine tract ... modulators of dopamine neurotransmission, and abnormalities in cannabinoid signalling or modulation of dopamine signalling or both have been implicated in a number of neurodegenerative diseases including ... levels of mutant huntingtin protein are lower in the R6/1 mice compared to R6/2 [10] and that neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... splicing, whereas a dashed line in exon of fragment C represents a deletion of 39 bp The thick lines indicate intron of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron in fragments C and ... contain the entire coding region Thus, use of alternative promoters in DNASE1 results in no generation of protein isoforms The 5¢-UTR of eukaryotic mRNA in uences the initiation step of protein ... for gene expression of vertebrate DNase I genes Sp1 is the founding member of a growing family of transcription factors that bind and act through GC boxes, being important in the transcription of...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... selectively and effectively induced maximal inhibition of Rho GTPase signaling [23,24] These data pinpoint to an important role for RhoA GTPase signaling in CTGF/CCN2 gene regulation Incubation of various ... F-actin bundles appear shorter and contained significantly less branching, consistent with the role of swinholide A as a promoter of G-actin dimerization Interestingly, both jasplakinolide and swinholide ... S1P-, anisomycin- and serum-induced CTGF/ CCN2 gene expression indicating that inducible CTGF/ CCN2 gene expression is independent of the Ras signaling pathway In contrast, exposure of the cells...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
Ngày tải lên :
19/02/2014, 16:20
... Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ... sites initiating biosynthesis of the 88, 77, 74 and 63 kDa iPLA2c isoforms Instead, based on current information about iPLA2c and its splicing, the first in- frame AUG site is downstream of the ... staining patterns (comparing and 2) and by the yellow color resulting from combination of green and red fluorescence in that iPLA2c is localized, in large part, to the mitochondrial compartment in...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... contains an N-terminal domain harboring activation function 1, a central DNA-binding domain (DBD) and a C-terminal ligandbinding domain (LBD) containing activation function [67–70] Upon binding androgens, ... Actin and ABPs in transcription regulation B Zheng et al Table Role of nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain ... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin...
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