strains culture conditions and expression of recombinant tbpb
Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
Ngày tải lên :
17/03/2014, 10:20
... proprothrombin), proFIX18 (residues )18 to )1 of proFactor IX), proFIX28 (residues )18 to +10 of proFactor IX), pro-e-TxIX/12 (residues )12 to )1 of e-TxIX precursor), e-TxIX12 (residues 1–12 of e-TxIX), ... of either the recombinant Conus carboxylase or the recombinant bovine carboxylase, and monitored carboxylation of FLEEL or e-TxIX12 as a function of propeptide concentration The effect of proFIX18 ... FLEEL, proPT28 and proFIX28, were Table Specific carboxylase and epoxidase activity of recombinant Conus carboxylase in the absence or presence of 400 lM proPT18 ND, not determined Expression system...
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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
Ngày tải lên :
23/03/2014, 09:20
... sequence Expression and purification of the recombinant 2-5A synthetase from G cydonium Expression and purification of the porcine recombinant 2-5A synthetase N-terminally 6xHis-tagged construct The recombinant ... purification conditions of the recombinant protein by means of changing pH of the lysis, wash and elution buffers Finally, protein purification was carried out under conditions in which cell lysis and ... Experimental procedures of the porcine 2-5A synthetase (data not shown) Figure demonstrates the results of the purification of the recombinant proteins The occurrence of dominant bands of the recombinant...
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Báo cáo Y học: Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity pot
Ngày tải lên :
31/03/2014, 21:21
... of insertion of the 29residue insert of murine PAPP-Ai corresponds to a junction between two exons of the human gene, and the nucleotides Expression and functional analysis of murine PAPP-A and ... PAPP-A and PAPP-Ai were made, cloned into a mammalian expression vector, and used for transient transfection of 293T cells The presence of recombinant murine PAPP-A in culture supernatants of transfected ... observed between human and murine PAPP-A Our cloning of cDNA encoding both PAPP-A and PAPP-Ai allowed expression in mammalian cells and functional analyses of the recombinant proteins Of particular interest...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
Ngày tải lên :
20/06/2014, 01:20
... Large-scale shaker flask cultures of E coli Rosetta strains expressing LASV NP and GP2 were performed in cLB medium supplemented with g/L of glucose, 100 μg/mL of ampicillin, and 35 μg/mL of chloramphenicol ... interests Luis M Branco, Alex Matschiner, and Darryl B Sampey are co-founders and sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, ... 0.2 g/L of uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43...
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Báo cáo hóa học: " Rift Valley fever virus structural proteins: expression, characterization and assembly of recombinant proteins" potx
Ngày tải lên :
20/06/2014, 01:20
... complex and assembled into a particulate structure in the absence of genomic RNA Figure Assembly of VLPs by expression of RVFV N and Gc proteins Assembly of VLPs by expression of RVFV N and Gc ... construction of recombinant baculoviruses, purification of proteins and VLPs, and EM studies CC carried out cell surface expression and cell to cell fusion studies PR contributed in the coordination and ... http://www.virologyj.com/content/5/1/82 Figure Expression of N, Gn and Gc proteins produced virus-like particles Expression of N, Gn and Gc proteins produced virus-like particles A) Sf9 cells were infected with the recombinant...
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Báo cáo khoa học: " Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays" doc
Ngày tải lên :
07/08/2014, 15:20
... Hind III, and analyzed in an 1.5% agarose gel Fig Expression of recombinant porcine IL-2 protein in E coli Expression of rpIL-2 was induced by the addition of IPTG into bacterial culture, and analyzed ... rpIL-2 and rhIL-2 in ELISA Porcine and Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays 211 human recombinant IL-2 was coated on the ELISA plate, and ... (Qiagen), and a clone containing IL-2 cDNA was selected and named as pQEIL-2 When the bacterial culture reached an OD600 of 0.6, the expression of IL-2 was induced by the addition of IPTG into...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
Ngày tải lên :
22/02/2014, 04:20
... other hand, it was inhibited by high concentrations of azide, an inhibitor of CD39 and most ecto-ATPDases [29–31], and high concentrations of fluoride, vanadate, and pyrophosphate, inhibitors of the ... absence and presence of the indicated concentrations of sodium azide.MgATP hydrolysis at pH 7.4 (s) and 6.4 (d); MgADP hydrolysis at pH 7.4 (h) and 6.4 (j) inhibition by azide was of the mixed and ... purification of the chicken liver ecto-ATPDase, its enzymatic properties and localization, cloning of the full-length cDNA and its expression EXPERIMENTAL PROCEDURES Materials The production and characterization...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
Ngày tải lên :
07/03/2014, 16:20
... the expression of this clone was highly induced in tissues of bacterial infected fish, a full-length cDNA clone as well as a genomic DNA clone were isolated and sequenced, and the expression and ... infection, and a strong product was present in samples prepared at 24 and 48 h after infection (Fig 4) Expression and modulation of trout IL-11 in cell lines The constitutive expression of IL-11 ... myofibroblasts, IL-b and TGF-b are potent inducers of IL-11 expression at both the mRNA and protein levels [8] Trout RTS-11 cells show constitutive expression of TGF-b mRNA, and IL-1b but not TGF-b...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
Ngày tải lên :
08/03/2014, 16:20
... pCR2.1 vector, and sequenced on both strands The cloned cDNA was 1370 bp long and contained an ORF of 1257 bp, encoding a protein of 418 amino acids with a calculated molecular mass of 46 751 Da ... injections of a solution containing 50 lg of lipid-free protein preparation in 0.9% NaCl mixed with an equal volume of Freund's complete adjuvant Boost injections of 50-lg protein were performed 21 and ... dithiothreitol, 40 UámL)1 of RNase inhibitor, 2.5 mM dNTP, 0.2 mM oligo-dT 15±18mer, and 200 U of M-MLV reverse transcriptase First-strand synthesis was performed at 42 °C for 45 and then the enzyme...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
Ngày tải lên :
16/03/2014, 16:20
... members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus and mammalian ... important domains within this APP superfamily member Expression pattern of Xenopus APLP2 mRNA The expression pattern of Xenopus APLP2 mRNA and the presence of alternatively spliced mRNA forms in various ... alternatively spliced exons Expression of APLP2 mRNA was detected in both neuronal and peripheral tissues of Xenopus (Fig 3) The presence of a 414 bp PCR product indicates the occurrence of the alternatively...
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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf
Ngày tải lên :
16/03/2014, 18:20
... speculated that the physical role of MeJA is to mobilize JA [19] The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa A recent study ... RT-PCR conditions were 30 at 50 °C, 15 at 95 °C; five cycles of 30 s at 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and ... Biochem 271) expression vector pQE70 (Qiagen) via SphI and BglII restriction sites Overexpression and purification Escherichia coli M15 cells harbouring the MJE expression plasmid were cultured at...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
Ngày tải lên :
18/03/2014, 01:20
... for both strands of the cDNAs and analysed by the DNASTAR program package (Lasergene) and CLUSTALW Plant treatments To determine the effects of several stress conditions on gene expression, D ... isolated two positive clones of 1324 and 1319 bp, designated DpAR1 and DpAR2, respectively The nucleotide sequences of DpAR1 and DpAR2 have been submitted to the EMBL database and are available under ... D purpurea Heterologous expression of DpARs in E coli The cDNAs of DpAR1 and DpAR2 were over-expressed in E coli as fusion proteins with His-tag (pQAR1 and pQAR2) The recombinant DpARs were purified...
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Báo cáo khoa học: A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin pptx
Ngày tải lên :
23/03/2014, 13:20
... (500 MHz) spectrum of the product of P450scc-mediated side-chain cleavage of 7-DHC (A) Spectrum of the enzymatic side-chain cleavage of 7-DHC; (B) spectrum of 7-DHP synthetic standard secondary ... rate of 0.5 mLÆmin)1 and temperature of 40 °C The mobile phase from to 30 consisted of 85% (v/v) methanol and 0.1% (v/v) acetic acid, and from 30 to 75 of 98% (v/v) methanol and 0.1% (v/v) acetic ... the Center of Excellence in Connective Tissue (to A.S and J.Z.) and Center of Excellence in Genomics and Bioinformatics (to A.S.), UTHSC, and NIH grants 1R01-AR047079-01A2 (to A.S.) and RR017854...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
Ngày tải lên :
30/03/2014, 13:20
... relative expression value of one The expression of both splice variants of L-fucokinase was found to be relatively high in brain, ovary, testis and kidney In spleen, heart and lung the expression ... time quantitative PCR assay [28] to detect the expression levels of pyrophosphorylase and long and short splice variants of fucokinase Primers and probes were designed using the PRIMEREXPRESS ... L-fucokinase and pyrophosphorylase The primer and probe sequences and their positions in the mRNA sequence, for GDP-L-fucose pyrophosphorylase and the short and long splice variants of L-fucokinase,...
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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
Ngày tải lên :
31/03/2014, 07:20
... result of evolutional divergence of transcriptional regulation Materials and methods Growth and maintenance of zebrafish adults and embryos Zebrafish were purchased from a local aquarium store and cultured ... major band of approximately 200 bp, and two minor bands of 250 bp and 180 bp, were observed after agarose gel electrophoresis of nested PCR products from the CIP/TAPtreated RNA No specific band was ... patterns of ILBP expression in fishes, the largest and most evolutionary diverse group of vertebrates FABPs have been detected in the white heart muscle of ocean pout and sea raven, the liver of nurse...
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expression of recombinant genes in eukaryotic systems
Ngày tải lên :
11/04/2014, 09:41
... ANALYSIS OF GENE EXPRESSION [2] lished in 1981 by Y Gluzman, have found widespread application for the rapid analysis of functionality of plasmids and for expression cloning and production of recombinant ... time point of transfection/infection, and the absence of mycoplasma and other contaminating agents needs to be controlled regularly The use of antibiotics and antimycotics in the cell culture medium ... proportionally and using unlabeled dCTP nucleotides The cDNA is then phenol:chloroform extracted and precipitated by the addition of 0.5 volumes of 7.5 M ammonium acetate and volumes of ethanol The...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
Ngày tải lên :
18/06/2014, 18:20
... body and CIMM Authors' contributions K.A.S., A.G., and R.G performed biological work J.G.P and A.S.K performed the clinical work, recruitment of patients, and procurement of specimens K.A.S and ... extended periods of time [5] Later reports from our laboratories included an analysis of the 5'UTR of CIMM-HCV [6] and the discovery of significant HCV variants [7] Rare insertions and deletions ... artefact of fixation and processing Figure gels of PCR products Agarose3 Agarose gels of PCR products The DNA standard is a kb plus ladder (Invitrogen) Arrows indicate the expected band sizes...
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Báo cáo sinh học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" docx
Ngày tải lên :
18/06/2014, 18:20
... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... amplification of plasmid T3 and the recombinant one (lanes and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic 2P viruses without and with the EGFP cassete (lanes and 6, respectively) ... the recombinant YF17D/Esa/5.1glic virus was capable of directing the expression of significant amounts of the heterologous protein even in cell cultures infected at low multiplicity (MOI of 0.02),...
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Báo cáo sinh học: "Safety and pharmacokinetics of recombinant human hepatocyte growth factor (rh-HGF) in patients with fulminant hepatitis: a phase I/II clinical trial, following preclinical studies to ensure safety" ppt
Ngày tải lên :
18/06/2014, 19:20
... platelet count of 9.2 × 104/μL, PT of 37% (PT-INR 1.78), T-Bil level of 11.7 mg/dL, ALT level of 260 IU/L, and serum albumin level of 2.9 g/dL Serum HGF and AFP levels were 1.07 and 3.9 ng/mL, ... days in patients and Patients and required cessation of rh-HGF on days 14 and 13, respectively, because of increased serum creatinine (2.1 mg/dL) and oliguria, respectively Both of these symptoms ... ALT and AFP, were measured before rh-HGF administration (day of rh-HGF dosing); on day of the rh-HGF dosing period; and one, seven and 14 days after the protocol therapy (days 1, and 14 of the...
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Báo cáo hóa học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" pptx
Ngày tải lên :
20/06/2014, 01:20
... body and CIMM Authors' contributions K.A.S., A.G., and R.G performed biological work J.G.P and A.S.K performed the clinical work, recruitment of patients, and procurement of specimens K.A.S and ... extended periods of time [5] Later reports from our laboratories included an analysis of the 5'UTR of CIMM-HCV [6] and the discovery of significant HCV variants [7] Rare insertions and deletions ... artefact of fixation and processing Figure gels of PCR products Agarose3 Agarose gels of PCR products The DNA standard is a kb plus ladder (Invitrogen) Arrows indicate the expected band sizes...
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