stepwise differentiation of embryonic stem cells for tendon tissue engineering

... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs)...

Ngày tải lên: 11/09/2015, 09:57

... Section I Differentiation of Mouse Embryonic Stem Cells [1] Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive Ectoderm-like (EPL) Cells By JOY ... use of FCS samples for the production of MEDII and formation of EPL cells in adherent culture The methods for production of MEDII and formation of EPL cells are given elsewhere in this review For ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of...

Ngày tải lên: 11/04/2014, 00:32

... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant source blastocysts otherwise selected for embryo transfer or ... production of cells for transplantation [27] Despite extensive experience with mouse embryonic fibroblast feeder cell layers for "culture support", exactly what these feeder cells provide for ESCs...

Ngày tải lên: 13/08/2014, 23:20

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing...

Ngày tải lên: 23/03/2014, 07:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... creating niches For this regulation, adhesion of stem cells to the ECM is crucial Cells and ECM adhesion molecules that enable cell communication are the prerequisite for tissue formation and maintenance ... tool for monitoring the modulation of mRNA profiles during differentiation processes This assay relies on the use of minor quantities of material (2 ng of total RNA/gene) and quantification of ... chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards...

Ngày tải lên: 09/08/2014, 10:20

... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... inhibition of JNK1, but not JNK2, sig- Page of 11 nificantly reduced the formation of Sox1+ and N-cadherin+ cells (Fig 5C, 5D and 5E), indicating that JNK1 is essential for the neural induction of ES cells ... demonstrated that the reduction of neural formation by the inactivation of MAPK was caused by the blockage of ES differentiation, rather than by the enhancement of formation of mesoendodermal nor epidermal...

Ngày tải lên: 10/08/2014, 05:21

... cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic precursor cells that ... mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse...

Ngày tải lên: 12/08/2014, 01:22

... adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) we differentiated ... of the cultures with the chondrogenic induction medium stimulated chondrogenesis (b) The formation of cartilage nodules consisting of large rounded viable cells (containing large quantities of ... effects of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of...

Ngày tải lên: 12/08/2014, 14:22

... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... for in vitro expansion has made it difficult to use broadly in the future clinical study Mesenchymal stem cells (MSCs) represent one of the most accessible sources of multipotent stem cells for ... hematopoietic and stem- like cells, for a variety of non-hematopoietic tissues The precursors for non-hematopoietic tissues were initially referred to as plastic-adherent cells or colony-forming-units fibroblasts...

Ngày tải lên: 11/09/2015, 09:05

... culture………………………………………………… 15 3.12 Human embryonic stem cell (hESCs) culture…………………………… 15 3.13 Differentiation of hESCs…………………………………………………………16 3.2 Characterization of Human Embryonic stem cells …………………………… 17 ... Determination of Genotoxicity using Mitomycin C…………………………… 26 iii CHAPTER 4 RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human ... Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure to low temperature……………….30 4.22 Spontaneous differentiation of hESC after exposure to low...

Ngày tải lên: 22/10/2015, 21:13

... of various stimulations on hepatocyte’s functions Finally, in the context of this liver tissue engineering study, the principles of tissue engineering and the status of current liver tissue engineering ... fabrication for liver tissue engineering 2-16 Scaffold surface modification 2-20 Tissue culture for liver tissue engineering 2-25 Coculture applications in liver tissue engineering ... strategies, tissue engineering applications on liver failure treatment, a survey of the prior arts of materials and scaffolds fabrication for tissue engineering, and the state of the art of BALs...

Ngày tải lên: 12/09/2015, 10:22

... the field of bone tissue engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, maintenance and substitution of damaged tissues and ... the understanding of the structure and cells which participate in bone repair is of utmost importance Bone is composed of around 70-90% of minerals with the rest in the form of proteins Within ... about 5000, second step of degradation will commence The rate of chain scission and weight of polymer decreases as a result of the formation and removal of short chains of oligomers from the scaffold...

Ngày tải lên: 09/10/2015, 11:18

... erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed ... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation...

Ngày tải lên: 07/03/2014, 12:20

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation- related...

Ngày tải lên: 18/06/2014, 15:20

... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA 2Department of Developmental Biology, Stanford University School of Medicine, Stanford,...

Ngày tải lên: 11/08/2014, 12:21

... Super-infection of ES cells by co-cultivation (a) Efficiency of infection by co-cultivation with the viral producer cell line B4-5 AB2.2, wild-type (WT), and NGG5.3 Blm-deficient embryonic stem cells cells ... an approach to generate cells with homozygous mutations, which increases the complexity of most genetic screens because of the low rate of loss of heterozygosity (LOH) of single allelic mutations ... analysis of this product revealed that the transcript of exon to from clone V3 was shortened by skipping of exon and part of exon (Figure 5d), providing an explanation for the viral resistance of this...

Ngày tải lên: 14/08/2014, 20:22

... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...

Ngày tải lên: 11/09/2015, 10:00

... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro ... GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies...

Ngày tải lên: 04/10/2015, 16:03

... Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their guidance, in ... days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells ... Feeder-Free Method Knockdown of Oct-3/4 and Sox-2 Transcription Factors in Mouse Embryonic Stem Cells a Establishment of Efficient Transfection Method b Oct-3/4, Sox-2 Knockdown and Differentiation Into...

Ngày tải lên: 27/11/2015, 11:25