starch biosynthetic enzymes and their isoforms

... cellulose- and xylan-degrading enzymes from a thermophilic microbial consortium obtained by enrichment of yard waste compost as a source Materials and methods 2.1 Chemicals and reagents All chemicals and ... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using Microsoft Excel and results were presented as average ± SD Results and discussion...

Ngày tải lên: 05/09/2013, 16:11

... enhances endocytotic internalization into the cells [20], and potentially impedes binding by RI Despite their clear cytotoxic action and their dimeric structure, however, RATEs were inhibited by ... (residues 51–57), a b-strand (residues 61–63) and a loop region (residues 75–79) of the N-terminal entity, and a-helix I¢ (residues 4¢–12¢), a loop region (residues 13¢–18¢) and the end of a-helix ... substrate] and cytotoxic (IC50 values between 12.9 lm and 40.0 lm with mammalian K-562 cells) All three variants showed thermodynamic stabilities similar to that of monomeric RNase A, and were...

Ngày tải lên: 22/03/2014, 16:21

... John Smith and Claire Simons Cardiff Univeristy Cardiff, UK Carbonic Anhydrase: Its Inhibitors and Activators Edited by Claudiu T Supuran, Andrea Scozzafava and Janet Conway Enzymes and Their Inhibitors: ... John Smith and Claire Simons © 2005 by CRC Press TF1766_title 7/22/04 2:53 PM Page CRC Enzyme Inhibitors Series Enzymes and Their Inhibition Drug Development Edited by H John Smith and Claire ... Cataloging-in-Publication Data Enzymes and their inhibition: drug development / edited by H John Smith and Claire Simons p.; cm — (Enzyme inhibitor) Includes bibliographical references and index ISBN 0-415-33402-0...

Ngày tải lên: 02/07/2014, 11:20

... for his guidance and support I would like to thank everybody at the lab for their advice and friendship It’s the best lab I have been in and everybody has been great They are past and present Guang ... being a good friend and helping me ever so often Xue Li for her expert assistance and comments Sravan for his advice and suggestions I am also indebted to Dr Robert Yang and all his lab members ... to thank my friend Lee for his support and encouragement The friendly office staff at DBS and Biochemistry and so many more people who have all gone out of their way to help me Thank you all Last...

Ngày tải lên: 04/10/2015, 16:03

... kox and kred are kcat values for alcohol oxidation and ketose reduction; KiNADH and KiNAD are dissociation constants for NADH and NAD+; and KmRO and KmROH are Michaelis constants for ketose and ... binding Discussion Kinetic mechanism of Af M1PDH and Af M2DH The theory developed by Cook and Cleland is used to deduce the kinetic mechanism of AfM1PDH and AfM2DH from KIE data in Table [28] The pattern ... enzyme From its sequence and three-dimensional structure, AbM2DH is classied as member of the short-chain dehydrogenase reductase superfamily of proteins and enzymes, and shows no signicant evolutionary...

Ngày tải lên: 14/03/2014, 23:20

... bonds involving C3 and C7 hydroxyl groups and lateral chains of Arg278 and Gln282, respectively; and (ii) p–p interactions between the thiazole ring and His229 In E3, the ligand is farther away ... Free energy, enthalpy and entropy for the drug-protein complexes computed at 300 K P1 and P3 refer to paclitaxel bound to human b-tubulin isoforms I and III, respectively I1 and I3 to IDN5390 bound ... IDN5390 and Leu217, His229 and Leu230, as well as between the C4 acetate and Phe272, Pro274 and Leu371 IDN5390 too is engaged in two hydrogen Docking of antimitotic agents to tubulin isoforms...

Ngày tải lên: 16/03/2014, 13:20

... recombinant chick Hsp90a and human Hsp90b are 0.025 and 0.015 min)1 (30 °C), respectively [42]; for recombinant human Hsp90a and 90% pure rat Hsp90b, they are 0.046 and 0.035 min)1 (37 °C), respectively ... human genome: insights into their divergence and evolution Genomics 86, 627–637 11 Sreedhar AS, Kalmar E, Csermely P & Shen YF (2004) Hsp90 isoforms: functions, expression and clinical importance ... [16] Results PP30[hHsp90a] and PP30[hHsp90b] ) yeast strains that express human Hsp90a or Hsp90b as their sole Hsp90 S cerevisiae strains PP30[pHSC82b], PP30[pHSP82] and PP30[hHsp90b] are hsc82D...

Ngày tải lên: 23/03/2014, 07:20

... tuberculosis H37Rv, and the PMM74 and the PMM56 mutant strains, and incubated for 20 h with continuous shaking Lipids were then extracted, and separated by TLC as described before, and labeled compounds ... digested with PacI and HindIII endonuclease restriction enzymes, and cloned between the PacI and HindIII sites of pMV361eHyg, a pMV361 derivative lacking the pHsp60 promoter and carrying a hyg ... OADC and incubation at 37 °C The values shown are the means ± standard deviations for three independent experiments important virulence factors for M tuberculosis, i.e DIMs and PGL-tb; and (b)...

Ngày tải lên: 23/03/2014, 09:20

... the expression of certain specific isoforms in various human tumors The mRNA of MT-1 series named as A, E, F, G, H, X and MT-3 isoforms but not MT-1B and MT-4 isoforms have been detected in breast ... function of MT1 and MT2 isoforms in papillary thyroid cancer cells (KAT5) demonstrated that KAT5 cells expressed eight functional MT1 and MT2 isoforms induced by cadmium Elevated calcium and activated ... indirect link between MT isoforms and tumor prognosis The expression of MT-I and MT-II (MT-I/II) isoforms were measured together with Western blotting, copper level, and lipid peroxides amounts...

Ngày tải lên: 09/08/2014, 01:24

... Expression of LMO2-L and -S isoform and activities of their promoters in haematopoietic and non-haematopoietic cells (A) Sketch map of three transcripts of LMO2 Lmo2-a/b which have and exons respectively ... (GIBCO BRL, Grand Island, NY, USA), 100 μg/ml penicillin and 100 μg/ml streptomycin Two millions of K562 cells were transfected by electroporation with a total of 10 μg plasmid DNA and electroporation ... U937, HL-60 and K562, the expression of both LMO2-L and -S was apparent, with LMO2-S always lower than LMO2-L Furthermore, the activity of LMO2-a, b and c promoters, termed P1, P2 and P3, respectively,...

Ngày tải lên: 10/08/2014, 05:21

... disinformation and propaganda – Deny service to legitimate computer users – Commit unauthorized intrusions into systems and networks that result in critical infrastructure outages and corruption ... malicious intent • Use their skills to expose security flaws Crackers • Person who violates system security with malicious intent • Have advanced knowledge of computers and networks and the skills to ... employees, and cyberterrorists • Password guessing is a basic attack that attempts to learn a user’s password by a variety of means • Cryptography uses an algorithm and keys to encrypt and decrypt...

Ngày tải lên: 17/09/2012, 10:43

... evaluate the predictors of clinical and pathological characteristics in these patients with steatosis and their diagnostic values in hepatic fibrosis Patients and methods Patients A total of 204 ... steatosis (83 men and 23 women, mean age: 41.08±10.23 years) and the remaining 98 patients were excluded from hepatic steatosis (79 men and 19 women, mean age 39.4±9.81 years) Their diagnoses were ... activity), and G3 (severe activity) All the sections were blindly and independently assessed by pathologists and the results were processed by the Kappa concordance test The inter- and intra-observer...

Ngày tải lên: 25/10/2012, 11:48

Ngày tải lên: 07/02/2013, 09:23

... Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Kawecka et al (2008), Wysokinska et al (2009), Pokrywka and Tereszkiewicz (2011) and Luc et al (2011; ... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement ... 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu and Piétrain boars Variable...

Ngày tải lên: 28/08/2013, 11:59

... River and their runoff characteristics (Ebise et al (2001, 2003, 2004 and 2006)) Here, pollutants contain organic matter, nutrients, SS and other measured substances Fig - Map of the Yodo River and ... loads, Proc of Environmental and Sanitary Research, (JSCE), 20, 27-38 Ebise, S., Inoue, T and Numabe, A (1993) Runoff characteristics and observation methods of pesticides and nutrients in rural rivers, ... composite sample and analyzed inorganic ions and organic matter The water samples taken at the three points spanning the river during the five flooding-stages were analyzed individually, and the representative...

Ngày tải lên: 05/09/2013, 09:38

... seaweed and aquaculture Midori Press, Tokyo, pp 133 - 144 Turner, M G., Gardner, R H and ÓNeill R V (2004) Landscape ecology in theory and practice: Pattern and process, Nakagoshi, N and Hara, ... from the bottom and thus, allowing the recovery of U pinnatifida and other non-encrusting macroalgae Both buoyed reefs were made of wood and their length was 1.0 m with 1.0 m width and 16 cm height ... macrophyte flora and infauna in the outer and inner areas of the bay A total of 16 macrophyte species in 2003 and 15 species in 2004 were observed in Line (Table 1) U pinnatifida and crustose coralline...

Ngày tải lên: 05/09/2013, 09:38

... (3) where NHi and NOi represent NH4-N and NO2+3-N in the ith layer [g-N/m2], respectively, and Nrice is the rice nitrogen [g-N/m2] The terms radv,NH,i and radv,NO,i are the NH4-N and NO2+3-N inflow ... production and water treatment and a detailed understanding of the nitrogen dynamics in VF constructed wetlands CONCLUSIONS In the experimental pots replicating VF constructed wetlands, a variety ... with growth Assuming Monod-type nitrogen uptake kinetics (Selim and Iskandar, 1981) and ignoring the temperature effects, the NH4-N and NO2+3-N uptake by rice is described as rup , NH ,i = k N ,up...

Ngày tải lên: 05/09/2013, 09:38

... and Environment Technology, Vol 9, No.2, 2011 In this study, runoff characteristics of materials such as organics, nutrients and inorganic matters from a citrus grove were investigated, and their ... in detail and material balances around reservoirs were considered RESEARCH PROCEDURE Location: Matsuyama region and periphery of the Seto Inland Sea The location of the research area and the distribution ... growth chamber under dark condition, and the temperature was maintained constant between 10°C and 30°C RESULTS AND DISCUSSION Materials runoff characteristics and variation in the reservoir Fig...

Ngày tải lên: 05/09/2013, 10:17

... indicator and the basis for calculations of heating and cooling energy demand [1] or for estimating bin data and heating and cooling degree-days at any base [2, 10, 11] Table shows the monthly and ... cooling demands is 28.5% and for Thessaloniki 13.2% Obviously, directly proportional to the energy demand for heating and cooling is the fuel (diesel oil) and electricity consumption and hence ... measures applied to dwellings Energy and Buildings 200, 37, 982-95 Frank Th Climate change impacts on building heating and cooling energy demand in Switzerland Energy and Buildings 2005, 37, 1175-85...

Ngày tải lên: 05/09/2013, 16:10

... - To describe the syntactic and semantic features of SVs in English and their Vietnamese equivalents - To find out the differences and similarities between ESVs and their Vietnamese equivalents ... 100% THEIR VIETNAMESE EQUIVALENTS 4.2.1 DEMAND, ORDER & REQUIRE and Their Vietnamese Equivalents Table 4.38 A Summary of the Meaning Nuances of DEMAND and Their Vietnamese Equivalents Verb English ... Literature Review and Theoretical Background; Chapter is Methods and Procedure; Chapter is Findings and Discussions; Chapter is Conclusions and Implications Chapter LITERATURE REVIEW AND THEORETICAL...

Ngày tải lên: 26/11/2013, 13:17