... interested in the C r -stability of mixing, and of the rate of mixing, for Axiom A and Anosov flows There is a quite extensive literature on mixing and rates of mixing for certain classes of Anosov flows ... the proofs of Propositions 4.8 and 4.9 are valid for f ∈ C 1,1 Proof of Lemma 4.2 Let Ψ ∈ V be sufficiently C -close to Ψ0 ∈ V∞ and let f ∈ C r (Σ1 ) Statements (1) and (2) of Lemma 4.2, and the ... Remark 1.10) (2) Parts (b) of Theorems 1.3 and 1.6 require only the density part of Lemma 2.2 278 ¨ ¨ MICHAEL FIELD, IAN MELBOURNE, AND ANDREI TOROK Openness and density of good asymptotics In this...
Ngày tải lên: 15/03/2014, 09:20
... at Tm The enthalpy of denaturation DHm of Ngb and horse heart Mb was 72 and 110 kcalÆmol)1, respectively Cytoglobin and the globin of Drosophila have lower values of DHm, 60 and 53 kcalÆmol)1, ... decrease of 20 °C in thermostability, again suggesting a critical role for His E7 in the enhanced thermal stability of Ngb Certain mutations of the distal histidine in Mb and Hb lead to instability ... native Ngb had negative bands at 208 and 222 nm (Fig 1), as expected for a high percentage of alpha helix Analysis of the secondary structure of Ngb gave 78% alpha helix and 22% of other forms, similar...
Ngày tải lên: 30/03/2014, 16:20
Methods to access quality and stability of oils and fat containing foods
... testing of oils and fat-containing foods Number of Samples The number of samples that can be evaluated in one session is dependent on the nature of the product and on the amount of training and experience ... Historical Glimpses of Analytical and Quality Assessment Methods for Fats and Oils H.J Dutton Factors Affecting Oil Quality and Stability T.H Smouse Sensory Analyses of Oils and Fat-Containing ... determining moisture, the “Swift stability test” bath, and the analytical balance A set of calibrated weights from the National Bureau of Standards were used, and tenths of milligrams were calculated...
Ngày tải lên: 23/05/2014, 12:34
Báo cáo hóa học: " Research Article Stability of Homomorphisms and Generalized Derivations on Banach Algebras" potx
... defined as in Theorem 2.2 Proof It follows from the assumptions that ϕ 0, 0, and so f is similar to the proof of Theorem 2.2 and we omit the details The rest of the proof Corollary 2.6 Let p, q, ... Proceedings of the National Academy of Sciences of the United States of America, vol 27, pp 222–224, 1941 T Aoki, “On the stability of the linear transformation in Banach spaces,” Journal of the Mathematical ... Journal of Inequalities and Applications 12 A Najati and M B Moghimi, Stability of a functional equation deriving from quadratic and additive functions in quasi-Banach spaces,” Journal of Mathematical...
Ngày tải lên: 22/06/2014, 02:20
Ensuring Financial Stability: Financial Structure and the Impact of Monetary Policy on Asset Prices
... prices “over and beyond” what such changes imply for the path of aggregate demand and inflation (Borio and Lowe 2002, Cecchetti et al 2000) Proponents of this policy emphasise that episodes of financial ... precision of the estimates, we thus follow Goodhart and Bean (2004) and Kohn (2006) discuss the implications of lags for the use of monetary policy in the face of asset price bubbles Sutton (2002) and ... for Denmark, Switzerland, Spain, Finland, France, Germany, Ireland, Italy, the Netherlands, Norway and the UK, a threemonth Treasury bill rate for Belgium, Sweden and the US, and a three-month commercial...
Ngày tải lên: 24/10/2012, 09:40
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... assembly and disassembly In this work, we studied the effects of chemical (i.e urea) and physical (i.e high pressure) denaturing agents in the structure and stability of the MS2 bacteriophage, and of ... fraction Fig Dissociation and denaturation of wild-type (WT) and mutant MS2 particles (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration To ... unaffected and showed only a 10-fold loss of titer Pressure stability of the MS2 bacteriophage and ts mutants Fig High-performance gel filtration chromatography of bacteriophage MS2 and the temperature...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... vant Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, ... The tting parameters for the cold and heat-denaturation data of 2.5 and 3.0 M urea are listed in Table 1, and Fig 5B ể FEBS 2004 Conformational stability of HPr of S coelicolor (Eur J Biochem 271) ... 14ị which are similar to Eqns 5, and 7, respectively Here, mmiDHi and mmiDSi are the values of mDHi and mDSi at the ể FEBS 2004 Conformational stability of HPr of S coelicolor (Eur J Biochem 271)...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
... half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively GABA values of NNN treated with dMM, and of ... trimming of N-glycans is involved in the regulation of the stability and trafficking of GAT1 It has been reported that a variety of molecular chaperones and folding enzymes assist the folding of newly ... activities of the N-glycosylation 1628 Fig Determination of expression of GFP-tagged GAT1 mutants on the surface of transfected CHO cells and measurement of GABA-uptake by GFP-tagged GAT1 wild type and...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... shows the fluorescence spectra of wild-type SNase and the four mutants E142O, K133A, W140A and W140O The fluorescence spectra of E142O and K133A are similar to that of the wild-type protein However, ... at 140 (W140A and W140O) was much lower than both the wild-type protein and mutants E142O and K133A Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants ... wild-type and mutant SNase Spectra of five proteins [wild-type (WT), W140A, E140O, W140O and K133A] Protein concentration was 0.4 mgÆmL)1 Fig Calometric melting curves of wild-type and mutants of SNase...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... analysis of the consequent hits was also performed to investigate the simultaneous improvement of the activity and stability of Symbiobacterium toebii TPL, as a better structural understanding of how ... basis of a co-improvement in stability and activity For example, the T1 ⁄ values for AS4 and AS6 were 6.7 and 11.2 °C higher, respectively, and the catalytic activities were improved by 2.8- and ... mutations from both the stability and activity mutants, and demonstrated a significant improvement in both stability and catalytic activity (Fig 1) As such, the activity and stability mutation combinations...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... characterization of Ngb and Mb at neutral and acidic pH (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved in 20 mM Tris ⁄ HCl and 0.15 M NaCl (pH 7.0) (B) Far-UV CD spectra of human apoNgb and horse ... acid denaturation of Mb, Ngb and H64Q mutant of Ngb was followed by measuring the decrease in intensity of the Soret band (Ngb, kmax of 412 nm; H64Q, kmax of 406 nm; Mb, kmax of 409 nm) in the ... concentrations of urea The urea denaturation of the holo forms of Ngb (open circles) and Mb (open triangles) was performed in 20 mM Tris ⁄ HCl and 0.15 M NaCl (pH 7.0), and that of apoNgb (filled...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
... (2005) Effects of submersion and aerial exposure on clutches and hatchlings of Pomacea canaliculata (Gastropoda: Ampullariidae) Am Malacol Bull 20, 55–63 Structure and pH stability of snail egg ... to be unpalatable [29], and in fact have a small number of predators The pH stability of ovorubin is within the pH range of vertebrate digestive tract fluids [30,31], and the present results indicate ... 4523 Structure and pH stability of snail egg ovorubin M S Dreon et al B A Y X X X Z Y C D log I (Q) 10 0.1 0.1 0.2 Q (Å–1) Fig Three-dimensional model of ovorubin from the eggs of P canaliculata,...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis doc
... mixture of A115 and F23, (ii) a mixture of A115 and F23, (iii) Y353 and (iv) A115 incubation at 22 °C for 24 h (Table 5) Mutants YQ + S327C, F1.2.A and F1.1.F + H355Y were again the most stable and ... [38] The stability of activity of purified WT and mutant CPUs were determined with the same protocol, but under defined buffer conditions of 50 mm Hepes, pH 7.4 Determination of the ORF of mutated ... [18,19] The study of this threshold phenomenon and, more generally, the study of the effect of CPU on fibrinolysis, are also severely complicated by its intrinsic instability of activity ‘Directed...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... difference of DHcal of mutant from wild-type (%), heat capacity (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants ... FEBS H.-M Chen et al Local stability and key acidic amino acids in staphylococcal nuclease Fig Steady-state fluorescent spectra of wild-type and mutants of SNase Spectra of nine proteins (WT, E73G, ... due to the loss of the hydrogen bonding among E75 and K9, Y93 and H121 [11] Instead, one water molecule hydrogen bonds to Y93 and H121 Both G75 and K9 are completely outside of this local bonding...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
... both in shape and in dimension to the fibrils of hTTR and sbTTR formed by heating The thick and straight morphology of heat-induced fibrils of hTTR, sbTTR and ecTRP is similar to that of amyloid fibrils ... E Lundberg et al Stability and fibril formation of TTR and TRP Fig DSC profiles of hTTR, sbTTR, and ecTRP The melting temperatures (Tm) were 97.8 °C for hTTR, 93.0 °C for sbTTR, and 66.7 °C for ... Tm of the protein These fibrils are amyloidogenic, as determined from their Stability and fibril formation of TTR and TRP tinctorial properties in the ThT-binding and CR-binding assays hTTR and...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf
... 1, molecular mass markers of 97, 66, 45, 31, 21 and 14 kDa Lanes and 3, G157D Lanes and 5, G376E Lanes 6, and 8, wild-type Fig Thermal denaturation of wild-type enolase and its variants, as monitored ... 56 and 49, between the dimeric and monomeric forms of enolase The loss of activity observed in most studies [23–25] on dissociation of enolase may be the result of changes in other regions of ... In view of the structural similarities between yeast and mammalian enolases and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx
... charge and steric effects may alter substrate binding and salt sensitivity In this study, the effect of NaCl concentration on the kinetic constants and thermodynamic stability of VsEndA and VcEndA ... the effects of reciprocal mutations of two non-conserved DNA binding site residues (VsEndA, Q69N and K71N; VcEndA, N69Q and N71K) on the kinetics, thermostability and salt dependence of these enzymes ... the stability and kinetic constants of VsEndA and VcEndA, and found the effects to be striking, especially for VsEndA Thermal stability At 175 mm NaCl, the native enzymes display a difference of...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... with standard globular proteins, the apparent molecular mass of holo-ACP dimer and the mixture of holo-ACP monomer and apo-ACP were found to be 33 kDa and 25 kDa, respectively Puried holo-ACP and ... Molecular mass determination of apo-ACP and holo-ACP Molecular masses of apo-ACP and holo-ACP were determined with an Ultra Flex TOF MALDI-TOF mass spectrometer (a) Mass spectrum of holo-ACP single major ... prole of apo-ACP and holo-ACP on a MonoQ HR anion exchange column Peak 1: apo-ACP Peak 2: holo-ACP (G) Separation of apo-ACP and holo-ACP; 12% native PAGE showing the separation of apo-ACP and...
Ngày tải lên: 16/03/2014, 10:20
Role of an electrolyte and substrate on the stability of porous silicon
... degradation of stability of PS on electrolyte (HF–C2H5OH and HF–H2O2) and current density formed on textured and polished Si substrates, respectively The emphasis is mainly 265 on the development of PS ... detection, and an Ar+ ion laser operating at 488 nm and mW (corresponding to 0.125 W cmÀ2) for excitation in all the measurements Decay of PL intensity has been used as a measure of the stability of ... I d (X50 mA cmÀ2) Decay of PL intensity is a good indication of the stability of porous silicon particularly of the surface bond configurations [3,16] In Fig 4(A), decay of the PL intensity at...
Ngày tải lên: 16/03/2014, 15:19
Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx
... calorimetric heats (Qt) of thermal transitions of pig muscle and yeast PGKs Tm and Qt values are given in °C and kcalÆmol)1, respectively Tm,cal and Tm,fluor were determined by DSC and fluorimetric experiments, ... W122 (e) and W333 (n) yeast PGKs were determined at the scanning rate of 1.0 KÆmin)1, in the absence of substrates (A, B) and in the presence of 10 mM 3-PG (C, D) In (A) and (C) the values of excess ... bonds N- and C-terminals N-terminus and bF Helix and C terminus bF and C terminus Within helix (C-terminal part) Helix (C-term.) and bJ bG and bH bG and bJ bK and bL Within helix 13 B stearotherm...
Ngày tải lên: 16/03/2014, 18:20