some structural and functional substrates of development in young cats

... containing Fronting and Khởi ngữ were categorized into functional and structural groups In the functional group, samples containing fronting were categorized into four minor groups: Linking with ... - Identify and point out the structural and functional features of Fronting in English and “Khởi ngữ” in Vietnamese - Find out the similarities and differences in the structural and functional ... functions and structures of fronting in English and khởi ngữ in Vietnamese 2.3 Data collection 50 sentences in English containing the fronting structure and 50 sentences in Vietnamese containing khởi...

Ngày tải lên: 28/02/2015, 11:54

... E185, D226, D244) in PIN domains involved in binding Mg2+ ions are conserved in all three sequences Other conserved residues are R263, V139 and N144 Determination of the MCPIP domain architecture ... proteins interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the ... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and...

Ngày tải lên: 18/02/2014, 14:20

... H pylori strains harbouring a functional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro -in ammatory production of cytokines, mainly interleukin (IL)-8 [12] ... the first and last turns of helices E and F, and the C-terminus helices I and J In addition, there is a lysine-rich N- and C-terminus, in accordance with the basic isoelectric point of CagS However, ... anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers also involves portion of chains D and E of the two monomers,...

Ngày tải lên: 06/03/2014, 00:21

... structures of CMP and UMP, and interactions between the cytosine moiety of nucleotide and various side chains of wild-type CMP kinase (Upper) Chemical differences between CMP and UMP are indicated in ... Calculating proton uptake release and binding free energy taking into account ionization and conformation changes induced by proteininhibitor association: application to plasmepsin, cathepsin D and ... with enzyme binding and substrate catalysis Kinetic and nucleotide-binding properties of E coli CMP kinase variants with ATP as variable substrate Determination of the reaction rates of different...

Ngày tải lên: 16/03/2014, 10:20

... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin, ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...

Ngày tải lên: 22/06/2014, 06:20

... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin, ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...

Ngày tải lên: 22/06/2014, 19:20

... apolipoprotein E J Biol Chem 2003, 278:40723 Gregg RE, Brewer HB Jr: The role of apolipoprotein E and lipoprotein receptors in modulating the in vivo metabolism of apolipoprotein Bcontaining lipoproteins ... their charge [43] In these experiments, the interaction of VLDL and apoE proteins increased the charge of VLDL particles, resulting in the migration of VLDL band (lane vs lane 3-4 in Figure 4) Remarkably, ... lipoprotein receptorbinding activity of the human E apoprotein due to cysteine-arginine interchange at a single site J Biol Chem 1982, 257:2518 Lane RM, Farlow MR: Lipid homeostasis and apolipoprotein...

Ngày tải lên: 10/08/2014, 05:21

... claudins ‘Non-classic’ claudins Claudin-10b Claudin-15 Claudin-9 Claudin-11 Claudin-6 Claudin-4 Claudin-18a Claudin-3 Claudin-18b Claudin-20 Claudin-12 Claudin-14 Claudin-2 Claudin-19b Claudin-19a ... Claudin-16 Claudin-7 Claudin-1 Claudin-21 Claudin-24 Claudin-22 Claudin-23 Figure A phylogenetic tree of full-length human claudin proteins, indicating the relationships between them Claudin-10, ... are just emerging and will probably become a major focus of research in the field of claudins and TJs From a practical point of view, a better under­ tanding of TJ s formation and regulation...

Ngày tải lên: 14/08/2014, 21:20

... by interacting with Upf3 (Lykke-Andersen et al., 2000) The crystal structure of the interacting domains of hUpf2 and hUpf3b shows that the RNA-binding domain (RBD) of Upf3 is involved in the interaction ... include the poly(A)-binding protein (Pab1) and the cap-binding protein (eIF4E) Pab1 couples decapping with deadenylation and inhibits decapping by promoting the formation of the translation initiation ... thereby linking the two domains together ix Electrostatic potential mapping combined with mutagenesis reveals that motifs I, V, and VI are involved in RNA binding In addition, trypsin digestion of...

Ngày tải lên: 14/09/2015, 22:22

... However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transition state stabilization during catalysis [17] A series of mutations ... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain ... main chain carbonyl Binding of a xanthine base from XMP in the same position, would lead to the loss of a hydrogen bond and a less favorable interaction To investigate this further, the protein...

Ngày tải lên: 15/02/2014, 01:20

... tight main-chain and medium side-chain restraints Model building was carried out in o [29] and coot [30] In chain A, residues and 169–176 are missing; in chain B, residues 167–172 are missing; in ... in chain C, residues 1, and 169–174are missing; in chain D, residues 168–174 are missing; in chain F, residues and 168–175 are missing All amino acid residues were present in the E chain, and part ... usually found in nucleotide binding enzymes is not present in UpUMPK [19] UpUMPK contains instead a glycine-rich motif within amino acids 44–54 that is responsible for binding of the phosphate...

Ngày tải lên: 18/02/2014, 16:20

... in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites were tested for interaction with the binding partner in ... biogenesis remains unknown In this study we mapped the mutual binding sites and assayed the effects of deletion and point mutations in Pex1p and Pex6p for the interaction of the proteins and for their ... interaction of both AAA-peroxins [23] How can these different functional roles of D1 and D2 of both AAA-peroxins for peroxisome biogenesis and interaction of the two proteins be explained? Our...

Ngày tải lên: 07/03/2014, 16:20

... shown in the presence of the actin crosslinking protein neurabin-2 (D) and an actin binding decient truncation mutant of neurabin-2 (E) in the lament cross-linking efciency of Iba1 and Iba2 or in ... phagocytosis [3] and to enhance the interaction of Iba1 with F-actin to a certain degree [11] In this study F-actin binding and cross-linking by Iba proteins was calcium independent, conrming previous ... morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar in the absence or presence of calcium ions Calcium binding in...

Ngày tải lên: 16/03/2014, 06:20

... containing similar quantities of 15R- and 15S-PG The physical organization of all corals, including P homomalla, comprises hundreds or thousands of individuals in a colony If a mixture of PGs ... assay the protein preparation was suspended in 50 mM Tris/HCl pH 8.0 containing lM hematin, mM adrenalin, and in some cases 0.5 mM SnCl2 was added The reaction was initiated by addition of 50 lM [1-14C]arachidonic ... R.D & Rhuland, L.E (1972) Occurrence of esters of 15S-prostaglandin A2 and E2 in coral J Am Chem Soc 94, 2122–2123 17 Light, R.J & Samuelsson, B (1972) Identification of prostaglandins in the gorgonian,...

Ngày tải lên: 23/03/2014, 12:20

... regulation of activities of substance P and related tachykinins [31], whereas cathepsin D is indispensable for protection of the onset and development of a certain type of neuronal ceroid lipofucinosis ... Degradation of a2-macroglobulin by cathepsin E (Eur J Biochem 270) 1191 Results Effect of a2M on the hemoglobin hydrolyzing activity of cathepsin E and cathepsin D To assess the inhibitory capacity of ... within a 15-min incubation at pH 3.8 and 37 °C (Fig 3A) The N-terminal amino-acid sequences of the 90- and 30-kDa peptides were found to be AVDGKEP and LAIPVE, respectively Within a 30-min incubation,...

Ngày tải lên: 23/03/2014, 21:20

... monomer bands of 18 kDa and dimer bands of 33 kDa To examine whether Ca2+ binding was required for dimerization, smelt AFP was incubated with BS3 in buffer containing 10 mM EDTA instead of CaCl2 In ... Ca2+-binding molecular mass standard b-lactoglobulin (not shown) The specificity of binding is demonstrated by the absence of detectable ruthenium red staining when CaCl2 is added to the staining and ... accumulation of a larger protein form With increasing concentrations of BS3, the disappearance of the monomer band corresponded to the gradual increase in intensity of a higher-molecular-mass band The...

Ngày tải lên: 24/03/2014, 03:21

... proteasome subunit S5a, and this interaction disrupts the interdomain contacts between the N-terminal ubiquitin-mimicking UBL domain and the two C-terminal ubiquitin-binding UBA domains, thereby inducing ... structure of the UIM of the Vps27 protein bound to ubiquitin [100] The C-terminal region of ataxin-3 including the polyQ region interacts with the N-terminal cofactor/substratebinding adaptor domain of ... C43/A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 of B as well as an ionic interaction...

Ngày tải lên: 30/03/2014, 15:20

... contain the actin-binding protein synaptopodin which is necessary for maintaining its laminar structure and for positioning it into spines (13) Only large hippocampal and cortical spines contain ... to ascertain the relative frequencies of free and bound polyribosomes in spines and at other synapse types in the developing and mature brain and during plasticity such as learning and memory ... volume of SER, suggesting a functional role in maintaining ionic balance of cerebellar spines The presence or absence of SER might also account for the relative stability of cerebellar spines, and...

Ngày tải lên: 06/07/2014, 15:30

... English and Vietnamese are very different English is a hybrid language consisting of some isolating features: prepositions, some inflecting (e.g the present tense of lexical verbs), and some agglutinating, ... knowledge of linguistics in general and that of idioms in particular Since language and culture are closely intertwined, the findings will help improve the knowledge of the two underlying cultures, which ... and sayings (e.g a bird in the hand is worth two in the bush (it is better to be content with something one has, or can be sure of, than risk having nothing at all by trying to get something more...

Ngày tải lên: 18/07/2014, 15:00

... assimilation in pine and studying regulation of the genes involved in the process [7] Two distinct but homologous nuclear genes for GS have been detected and colocalized in the pine genome [2] both of ... of DNA-protein interactions in the 5’flanking region of GS1a gene from Scots pine MATERIALS AND METHODS 2.1 Isolation of a genomic clone containing GS1 sequences in pine Scots pine genomic DNA ... contained the 5’ half of the gene including part of the coding region organized in seven exons, interrupted by introns and 980 bp upstream the translation initiation codon (figure 1) The sizes of...

Ngày tải lên: 08/08/2014, 14:20