separation of hemoglobin and cytochrome c by horizontal agarose gel electrophoresis

... reference to PH c5 52, which has been characterized as a System I-dependent cytochrome c Biogenesis of cytochrome c Heterologous synthesis of PHCP by E coli The E coli System I cytochrome c biogenesis ... thermoluteolus cytochrome c (PHCP) and H thermoluteolus cytochrome c5 52 (PH c5 52) genes are indicated as c and c5 52, respectively The arrow and arrowhead indicate the positions of the PHCP and PH c5 52 ... Ministry of Education, Culture, Sports, Science and Technology of Japan References Ambler RP (1982) The structure and classification of cytochromes c In From Cyclotrons to Cytochromes (Kaplan NO &...

Ngày tải lên: 06/03/2014, 00:20

... effect of MccJ25 on the respiratory chain forces the opening of the MTP pore, which is mediated by an increase of the matrix calcium concentration, with the concomitant release of cytochrome c ... Effect of different drugs on the swelling and calcein release from mitochondria induced by MccJ25 Swelling and calcein release of energized mitochondria were induced by 20 lM MccJ25 and 50 lM Ca+2 ... induced by MccJ25 The inhibition of swelling induced by ascorbic acid will be discussed later As a positive control, the swelling and calcein release induced by calcium, and the inhibitory effect...

Ngày tải lên: 16/03/2014, 06:20

... Findings and Discussion It is concerned with the discourse features of American English and Vietnamese opening and closing speeches in terms of the layout, syntactic features, lexical choice and cohesive ... syntactic features and cohesive devices They were then compared to see the same and the different characteristics of ACSs and VCSs The same specific tasks done with AOSs and VOSs were conducted ... analyzing, comparing and contrasting the layout, lexical choice, syntactic features and cohesive devices in AOSs versus VOSs and ACSs versus VCSs The results marked the significant influence of culture...

Ngày tải lên: 26/11/2013, 12:41

... correlation of contacts with a doctor in EU countries 52 35 Regression of contacts with a doctor in EU countries 53 36 Inpatient acute care occupancy rate 54 37 Number of persons ... health and functional characteristics and on the other hand to study the influence of the socio-economic variables As could be expected, a higher education level leads to a healthier behaviour and ... two-way correlation in EU countries*, 2000 and 2001 Hospital days Coefficient Significance Hospital days of inpatients Coefficient Significance Good health status Coefficient Significance 2000...

Ngày tải lên: 05/03/2014, 18:20

... exclusion 21 21 21 Number of accepted samples 105 105 104 Accuracy (specificity), % 100 — — Accuracy (sensitivity), % — 98,1 99 Accordance, % 100 96,2 98,1 Concordance, % 100 96,2 98,1 Table C. 3 ... laboratories Number of laboratories retained after exclusion 25 Number of accepted samples 125 Accuracy (specificity), % Accuracy (sensitivity), % 94,4 Accordance, % 88,8 Concordance, % 26 — 89,1 ... 37 C and 55 C 6.3 Incubator, capable of operating at 37 C ± C 6.4 Water bath, capable of operating at 41,5 C ± C, or incubator, capable of operating at 41,5 C ± C 6.5 Water baths, capable...

Ngày tải lên: 07/03/2014, 16:20

... Carlsbad, CA, USA) by PCR using sense primer 5¢-ATCAAAACAACCAAAA TGGCCAAGTTGACCAGTGC-3¢ and antisense primer 5¢-GAATGCGGCCGCTCAGTCCTGCTCCTCGGCCAC-3¢, which introduced a NotI restriction site ... primer 5¢-CAGCTAAACCCAATAGTCTG-3¢ and sense primer 5¢-GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGI IG-3¢ Thirteen cycles of PCR amplification (12 s 94 C, 15 s 55 C, 1.5 72 C) followed by 33 cycles (12 ... site of pUC18 and sequenced, resulting in pUC18 ⁄ fcp1.9kb (covering 278 bp of fcp coding sequence preceded by 5¢-UTR) and pUC18 ⁄ fcp1.46kb (covering 357 bp of the fcp coding sequence followed by...

Ngày tải lên: 07/03/2014, 21:20

... CHAPTER CLASSES, CHARACTERS,, II AND KINDS OF Music Identity of Class in Sacred and Secular Distinction of Character Necessity _for Forms tion Homophonic Sacred and strumental, amples and Secular ... Staccato Position and At tack Each Production of the Octave Staccato Development of the Muscles Used in Staccato Play 204 215 m ing IV THE TWO-FINGER EXERCISE Symmetry Technical Practice Value of ... Nature and Effect of Music Audiences of To-day Sense of Rhythm Appreciation of Tune Con Con trast in Melody Taste for Classical Music Influence of cert Reform Music in the Home Surroundings Beneficial...

Ngày tải lên: 15/03/2014, 13:44

... in uncertainty The earliest practice of medicine was undoubtedly theurgic, and common to all primitive peoples The offices of priest and of medicine-man were combined in one person, and magic was ... Disease receive the Church's Sanction Monastic Medicine Miracles of Healing St Paul St Luke Proclus Practice of Anatomy denounced Christianity the prime factor in promoting Altruism CHAPTER XII ... Alexander of Tralles The Plague Moschion Paulus Ægineta Decline of Healing Art CHAPTER XI Influence of Christianity on Altruism and the Healing Art Essenes Cabalists and Gnostics Object of Christ's...

Ngày tải lên: 15/03/2014, 15:20

... expressed in bacteria (prokaryotic: PrPhl p 1) indicated a considerable amount of unordered structure The characteristics of the CD spectrum of insect cellexpressed Phl p indicates structural similarity ... Phl p by circular dichroism spectroscopy Far-UV CD spectra of E.coli- (grey) and insect cell-expressed Phl p (black), expressed as mean residue ellipticity (y-axis), were recorded at 20 C in ... purchased from Pharmacia Diagnostics Construction of recombinant baculovirus The Phl p 1-encoding cDNA [16] was PCR amplified and cloned into the BamHI and KpnI restriction sites of the pBacPAK8...

Ngày tải lên: 16/03/2014, 18:20

... Dewey Decimal Classification system, we can assign the 500-599 pure sciences section, and in the Library of Congress classification the call letter "Q" for science can be used For a specific element/quality ... 1954, 1), and " knowledge of the culture of Africa has been lost because of the destruction of ancient records," (Jackson, 1970, 296); Kemetic Roots of Library and Information Science, by Itibari ... education, architecture; (7) Melvil Dewey and his classification scheme; and (8) cataloging, we can turn to the issue of miseducation, misinformation, and an affirmative Afro-centric corrective action...

Ngày tải lên: 28/03/2014, 18:20

... mismatched bases are underlined: 5¢-ATCGAAGCC GAGCGCGTTTCC-3¢ (R234A); 5¢-GAACGCGAGGCC GTTTCC-3¢ (R236A); and 5¢-GATATCTGCGCCGT GCTC-3¢ (R242A) A 1400-bp fragment of the StP gene, obtained by digestion ... reading frame, pNter (5¢-CGCGCATGCCTGAAAAACAGCCACTCC-3¢), pCter (5¢-ACGGCATGCTTAAACAGCAGGAGTTGG-3¢), where restriction sites are underlined The resulting recombinant StP lacked Ser1 Ó FEBS 2003 ... in rmGP), were chosen and reverse translated into a pair of degenerated PCR primers: forward (p1), 5¢-GG (ACT)AA(CT)GG(GCT)GGT(CT)T(AG)GG(ACT)CG pNter (5¢-CGCGCATGCAGCCCTGAAAAACAGCC-3¢) derived...

Ngày tải lên: 31/03/2014, 01:20

... investigated the kinetics of lysosomal downregulation of CD4 and CXCR4, in the presence and absence of Gag CD4 and CXCR4 function as the receptor and co-receptor respectively for the entry of HIV-1 X4 variants ... von Schwedler (University of Utah School of Medicine, Salt Lake City, UT) siRNAs directed against human TSG101 (5'-CCUCCAGUCUUCUCUCGUCTT-3') and LacZ were obtained from the High Throughput Screening ... human T lymphocytes Evidence for distinct endocytic routing of CD4 and CD3 J Biol Chem 1992, 267(26):18837-18843 Weyand CM, Goronzy J, Fathman CG: Modulation of CD4 by antigenic activation J Immunol...

Ngày tải lên: 20/06/2014, 01:20

... to economic and social aspects and capacity requirements of policy, so implementation can be ineffective and sometimes even causes economic losses People/farmers are very seriously affected by ... standards Training in Good Management Practices and HACCP could be offered to small/domestic firms, accompanied by a schedule for adoption of which ever standard is more appropriate for specific ... policy advice On agriculture and rural issues, MARD will issue official documents to specify objectives, requirements and working content and send these to an “in-charge” office for classification...

Ngày tải lên: 21/06/2014, 05:20

... the choice of consumers and farmers between conventional, organic and GM crop production Thus, the aim is to accomplish a spatial segregation between GM and non-GM production at the landscape ... International implications of commercialization (Transgenic Crops) in the ecological risks of engineered crops Cambridge: MIT Press; 1996 55 OECD (Organisation for Economic Co-operation and Development): ... source products are prescribed to be free of charge and the source code is disclosed and free for modifications Open source software is not confined to private use, but is adopted from business companies,...

Ngày tải lên: 21/06/2014, 06:20

... industry and commerce and labour unions on the economic policy of the Federal Republic These few examples may suffice to describe some of the actual projects of the Cologne research workers on socio-economic ... psychologists Some of those meetings resulted in direct contacts and stimulated our socio-economic research.68 The problem of consistency and change in the economically relevant behaviour of ... cent of one-account households have no bank account at all, compared with only 19 per cent of households with two accounts; 40 per cent of one-account households but only 29 per cent of twoaccount...

Ngày tải lên: 21/06/2014, 08:20

... without a closer examination of his circumstances and of the motivations which guide his actions.20 Accordingly, empirical analysis of saving has always sought to create operational concepts and delimitations ... above by only per cent of the heads of households which largely not engage in planning, but by 32 per cent of heads of households with high use of calculation Accordingly, one can also consider ... or vice versa), decisions about the ways of saving and means of capital accumulation and, last but not least, the specific techniques employed in implementing such decisions (cash, cheque, credit,...

Ngày tải lên: 21/06/2014, 08:20

... saving, by account type (%)a Purpose of account Postal savings account Savings bank account Bank account 65 18 16 45 39 37 41 41 33 Consumptive saving Precautionary saving Capital accumulation ... specific attitudes and expectations of individuals any projection and forecast becomes feasible Feasibility given, the achievement of accuracy in predicting is yet another and quite complicated ... bank cheque accounts are even more mobile than commercial bank accounts Precisely on this point, i.e mobility of account types, we asked the owners of cheque accounts whether they usually paid by...

Ngày tải lên: 21/06/2014, 08:20

... As such, and as the pillar of economic and technological progress and therefore of economic development, the Schumpeterian entrepreneur truly forms a fourth factor of production alongside land, ... holder of the public office whose task is ‘to examine national requirements and to induce national production accordingly’, and he accurately depicted the feedback function of the entrepreneur ... the Cologne Institute of Public Finance and the attached Centre of Empirical Economics Research 157 national parliament, the Deutsche Bundestag, was tested by modern methods of opinion research...

Ngày tải lên: 21/06/2014, 08:20

... judgement, and more of a mix of often vaguely sensed, and sometimes even contradictory feelings of justice This imbalance, and even contradiction, in the prevailing ‘conventions of justice’ does ... political will and administrative practice It cannot acknowledge notions of justice at the ‘higher’ level of politics and public opinion and overlook the more blunted feelings and resentments of citizens ... exceed by far those found in other countries The logical consequence of such relatively coercive tax enforcement techniques is the high degree of alienation between citizens and the state and...

Ngày tải lên: 21/06/2014, 08:20

... character of the peoples concerned Econometrics, which concerns itself with the observation and measurement of economic cycles and with the interaction of macroeconomic factors, derives its conclusions ... any variation of global aggregates This approach consciously leaves behind the ‘capital pool’ concept which explains economic stagnation by the scarcity of capital and other resources.87 Rather, ... manufacturers must make regarding the direction and scope of production Consequently, business forecasting, more than any other branch of economic research, must remain a science of predicting...

Ngày tải lên: 21/06/2014, 08:20