selection of echo reflected by the intended target
Báo cáo sinh học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" doc
... rapid approach for the generation of recombinant MVA [43,44] While the BAC technology offers the advantage of targeting any genomic locus of MVA, the method is dependent on the use of a helper virus, ... viruses of altered plaque properties or narrowed host range, allowing the selection of recombinants after re-introduction of these genes and rescue of the wild-type phenotype While the latter selection ... procedure, the absolute titer of the defective virus is not critical However, the growth kinetics of dMVA suggest that the expression level of the vaccinia uracil DNA glycosylase by the cell line...
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Báo cáo hóa học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" docx
... rapid approach for the generation of recombinant MVA [43,44] While the BAC technology offers the advantage of targeting any genomic locus of MVA, the method is dependent on the use of a helper virus, ... viruses of altered plaque properties or narrowed host range, allowing the selection of recombinants after re-introduction of these genes and rescue of the wild-type phenotype While the latter selection ... procedure, the absolute titer of the defective virus is not critical However, the growth kinetics of dMVA suggest that the expression level of the vaccinia uracil DNA glycosylase by the cell line...
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Tài liệu Audit of Contract Management by the Sectors doc
... case, we asked the PWGSC contracting officer why the 10% profit invoiced by the subcontractor had been approved when there was no mention of it in the basis of payment The contracting officer told ... that despite the fact that no mention of profit was made in the basis of payment, it was the intention of the contracting authority to authorize such a profit The PWGSC contracting officer told ... conditions of the contract When we examined the 27 contracts, we compared the request for payment with the contract’s basis of payment to see if the two matched The audit showed that for 23 of the 27...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
... amounts of SE (A) Quantification of SE in cell polarity mutants Cells of the indicated strains were grown to stationary phase and then lipids were extracted and separated by TLC The amount of SE of the ... affect sterol biosynthesis during the formation of a mating projection Nevertheless, the phenotypes of mutants defective in ergosterol synthesis clearly demonstrate the importance of sterols for polarization ... RUra3, which is fused to the PAK Released RUra3, a modified version of the enzyme Ura3 with an arginine at the extreme N-terminus, is targeted for degradation by the enzymes of the N-end rule A protein–protein...
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Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx
... for the indicated times in buffer F (DAPA was included to inhibit the feedback proteolysis of prothrombin by the generated thrombin) The reaction was stopped by the addition of an equal volume of ... activation of human prothrombin was determined by measuring the generation of thrombin using the chromogenic substrate Spectrozyme TH, in the presence of the indicated concentrations of plactin ... conversion of scu-PA to the two-chain form The apparent molecular masses of the resulting polypeptide chains were comparable with those of the A- and B-chains of tcu-PA (an A-chain doublet was caused by...
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Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx
... suggested that the N-terminal a-helix of CB1 is located on the surface of the lipid bilayers, whereas the C-terminal a-helix of CB1 is embedded below the surface of the lipid bilayers These conclusions ... sample loss caused by the adsorption of hydrophobic molecules to the surfaces of the flow system of the BIAcore instrument Peptides dissolved in HBS-EP buffer are not lysed by the liposomes Avidin ... DSC analysis of the effect of peptides on PtdEtn/PA LUV thermotrophic phase behaviour The effect of CB, CB1 and CB3 on the thermotrophic phase behaviour of LUVs is shown in Fig The phase-transition...
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... structures of the b-amino acids that best fit are shown in Fig They all correspond to gauche(+) values of the h angle The methyl group of b2-HAla occupies a position close to that of CH2b of Pro or the ... the expense of a minimal decrease in its activity HGly (named by the authors b-alanine) has been previously introduced in the sequence of the C-terminal heptapeptide of NKA, another peptide of ... w) values of the selected Pro conformer are ()72°, 153°) The low-energy conformers of b-HAla generated in the grid calculation have been selected on the basis of the best fit with Pro They correspond...
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REGULATION OF INTEREST RATE BY THE STATE BANK OF VIETNAM (SBV) potx
... instead of lowering the interest rate according to the policy adopted by the Conference of Bankers at the beginning of the year, the State Bank preserved the status quo on the grounds that the inflation ... determined by the central bank and announced on the basis of real situation and targets of the national monetary policy The point 12, Article of the Law on the State Bank of Vietnam stipulates: " The ... among the banks Deposit side vs Lending side: The utility package offered by the banks to the depositors mainly includes the interest rate, the quality of service, and the credibility If the banks...
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Listening and Learning: the Ombudsman’s review of complaint handling by the NHS in England 2010-11 doc
... periods of great anxiety about the terminal illness of a loved one or the health of a young child In one case, the decision to remove the patient was made by the member of staff involved in the altercation ... with national leaders, from the Chief Executive of the NHS to the leaders of the professional bodies and regulators Our report was quickly followed by the CQC’s programme of unannounced inspection ... consider other courses of action the response They asked the The Practice also took Miss F’s Ombudsman for help Miss F said mother off their list even though 18 she had not been involved in the disagreement...
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Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot
... width of 1.0 mm The wavelength range of the spectrometer was 370– 820 nm Experimental conditions are described in the legend of each figure R E S U LT S Formation of glyoxal in the reaction of linoleic ... linoleic acid by MnP in any of the buffer systems (Fig 1) Syn and q FEBS 2001 anti-isomers of these PFBO derivatives were separated on the GC/MS column The mass spectrum of PFBO derivatives of glyoxal ... PFBO derivatives of glyoxal formed by the oxidation of linoleic acid with MnP (A) and authentic standard (B) (A) Glyoxal formed by the oxidation of linoleic acid with MnP for 19 h The reaction system...
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Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt
... indicating that these RNAs are not efficiently processed or degraded by the exosome in the absence of Nop53p In order to determine whether the effect of Nop53p depletion on 5.8S processing by the exosome ... degrade RNA by itself, its presence stimulates the RNase activity of the exosome It is possible that the stimulation of the exosome activity is due to recruitment of the complex to the substrate ... extension of the 7S pre-rRNA and formation of the mature 5.8S rRNA [13] Interestingly, despite being directly involved in the late steps of processing, depletion of essential subunits of the exosome...
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báo cáo hóa học:" Transcriptional regulation of bone formation by the osteoblast-specific transcription factor Osx Chi Zhang" pptx
... expression of type I collagen (Col1a1) in the condensed mesenchyme of the membranous skeleton and the periosteum and mesenchyme of the endochondral skeleton is severely reduced Expressions of the osteoblast-specific ... regulator of postnatal bone mass 11 Dlx5 delayed ossification of the roof of the skull and abnormal osteogenesis positive regulator in OB differentiation 12 reading frame (ORF) and 3-prime UTR The ... Osx-null embryos the number of TRAP-positive cells appear to be reduced compared to wild-type embryos In long bones of Osxnull embryos cells from the periosteum invade the zone of the hypertrophy...
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Báo cáo hóa học: "Synthesis of SnS nanocrystals by the solvothermal decomposition of a single source precursor" pdf
... Values of the optical band gap for the samples were obtained by the extrapolation of the linear region of the plot of (ahv)1/2 against photon energy (hv) as shown in Fig 4(c) Clearly, the absorption ... to be dependent on the nature of the stabilizing agents used in addition to the thermal conditions of the preparation Isotropic and anisotropic growth of SnS NCs is achieved by use different capping ... the synthesis of high quality semiconductor SnS NCs due to its low cost and low toxicity As indicated by the thermoanalytical data [13, 14], the substantially complete thermal decomposition of...
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Báo cáo hóa học: " Synthesis of SnS nanocrystals by the solvothermal decomposition of a single source precursor" ppt
... Values of the optical band gap for the samples were obtained by the extrapolation of the linear region of the plot of (ahv)1/2 against photon energy (hv) as shown in Fig 4(c) Clearly, the absorption ... to be dependent on the nature of the stabilizing agents used in addition to the thermal conditions of the preparation Isotropic and anisotropic growth of SnS NCs is achieved by use different capping ... the synthesis of high quality semiconductor SnS NCs due to its low cost and low toxicity As indicated by the thermoanalytical data [13, 14], the substantially complete thermal decomposition of...
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Designation: C 115 – 96a - Fineness of Portland Cement by the Turbidimeter1 pps
... The zero of the scale shall indicate that position at which the center lines of the slots for the light beam are at the same elevation as the surface of the liquid in the tank when filled to the ... adjusted so that the turbidity of the suspension may be determined at any desired depth The center of the light source, the heat absorbing device, the photocell, the center of the slots of the metal ... past the corresponding mark on the buret 8.3.8 Lower the shelf to the 30–50 µm position Replace the filter in the path of the light beam, remove the tank, and check the intensity of the lamp If the...
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Designation: C 184 – 94e1 - Fineness of Hydraulic Cement by the 150-µm (No. 100) and 75-µm (No. 200) Sieves1 ppt
... and the beads lodged in the meshes are difficult to remove Tapping the frame of the sieve and gentle brushing of the cloth from the underside will aid in recovering all the beads and keep the ... important that the sample for calibration be of the same typical fineness as the range at which the sieve is to be used Determine the percent residue of the test material on each of the three sieves, ... through the center If one of the lines is marked with an arrowhead and the habit of starting with this point under the right hand has been formed, one can easily follow the progress of the 1-min...
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Designation: C 430 – 96 - Fineness of Hydraulic Cement by the 45-µm (No. 325) Sieve1 pps
... at the rate of one motion per second in the spray The bottom of the spray nozzle should extend below the top of the sieve frame about 12 mm (0.5 in.) Immediately upon removing the sieve from the ... determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility This standard is subject to revision at any time by the responsible ... (0.51-mm) Holes Calculation 7.1 Calculate the fineness of the cement to the nearest 0.1 % as follows: proportional to the amount of residue Example of Determination of Sieve Correction Factor: Residue...
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accomplishment of largescaled inspection by the government inspectorate in the implementation of social policies
... in the implementation of social policies Structure of the research theme - Chapter 1: Theoretical basis of the large-scaled inspection by the Government Inspectorate in the implementation of ... Apart from the effect of reorganizing the operation, compliance with the laws, the policies of the Communist Party of Vietnam and the State of Vietnam, large-scaled inspection by the Government ... agencies, one of three to-do works of the leadership of the Communist Party of Vietnam and governments at various levels, an integral part of the leadership of the Communist Party of Vietnam and the State...
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Báo cáo sinh học: "Mechanisms of ubiquitin transfer by the anaphase-promoting complex" pdf
... Finally, the E3, or ubiquitin-protein ligase (green), facilitates the transfer of the ubiquitin from the E2 to a lysine on the target protein (substrate, magenta) In the case of the APC and many other ... thioester bond between the active-site cysteine of the E1 and the carboxyl terminus of ubiquitin The E1 then transfers the activated ubiquitin to the active-site cysteine of an E2, or ubiquitin-conjugating ... member of the RING-domain family of E3s These proteins facilitate the final step in ubiquitination by positioning the E2-ubiquitin conjugate next to the substrate, allowing the ε-amino group of a...
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Global orchestration of gene expression by the biological clock of cyanobacteria Carl Hirschie Johnson pot
... not the only target of KaiC, however; the recent paper by Nakahira and coworkers [16] reports the unexpected result that KaiC overexpression represses the rhythms of all promoters in the S elongatus ... [15] and the lab of Susan Golden [21], in addition to the study of Nakahira and coworkers [16], are consistent with this hypothesis, namely that the condensation or supercoiling status of the cyanobacterial ... identified two classes of response to KaiC repression The first class, termed ‘high amplitude’ by Nakahira and coworkers [16], was exhibited by 5-10 % of the promoters, including kaiBCp; these promoters...
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