schizophrenia and the alpha7 nicotinic acetylcholine receptor
Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
Ngày tải lên :
19/02/2014, 07:20
... homomeric receptor In these receptors, there are five identical interfaces that presumably play equivalent roles in ligand recognition and receptor function In the heteromeric receptors, the roles of all ... of cW55 and dW57 in the Torpedo nAChR have been shown to affect the affinity for dTC and ACh [17,22] while the W54 of the neuronal nicotinic a7 receptor has been shown to contribute to the binding ... photoincorporation in the c-subunit of the Torpedo nicotinic acetylcholine receptor FEBS Lett 423, 223–226 17 Xie Y & Cohen JB (2001) Contributions of Torpedo nicotinic acetylcholine receptor cTrp-55 and dTrp-57...
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Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx
Ngày tải lên :
21/02/2014, 03:20
... from the Applied Biosystem standard Fmoc 0.1 mmol small-scale program [42] At the end of the synthesis, the peptide was cleaved from the resin and the protecting groups were removed from the amino ... display 11 residue differences and that the competition systems used (human and rat on one hand, and chicken on the other) are not identical in the two studies, the two toxins appear to behave ... a7)5HT3 receptor expressed in HEK cells [23], and 125I-labelled a-Bgtx as a tracer The deduced Kd with Torpedo receptor was estimated to be 180 nM (Fig 5, Table 1) With the a7 neuronal receptor, the...
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Báo cáo y học: "Expression of the α7 nicotinic acetylcholine receptor in human lung cells" pdf
Ngày tải lên :
12/08/2014, 18:21
... for the α7 nicotinic acetylcholine receptor, it was necessary to show if expression of the α7 nicotinic acetylcholine receptor protein in seen in these cell lines Using a specific antibody for the ... laboratory and others In addition, it has been shown that the NCI-H82 cell line synthesized the highest levels of acetylcholine, and addition of nicotinic antagonists slowed growth of the NCI-H82 ... pronounced upregulation of the α7 nAChR in the lungs of the newborns [24] Together these data indicate that the α7 nAChR may play an important role in the development of SCLC, and other lung cancers in...
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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
Ngày tải lên :
19/02/2014, 12:20
... for the development of selective drugs is the identification and pharmacological characterization of the various receptor subtypes, and the determination of their precise subunit composition and ... on the number of amino acids between the second and the third cysteine residues (loop I) and the third and the fourth cysteine residues (loop II), respectively [32] (Table 1) It appears that these ... agonist-evoked catecholamine release a6/a3 and a7/5HT3 indicate chimeric receptors between nicotinic a subunits and nicotinic a7 and the 5-hydroxytryptamine receptor, respectively a7/5HT3 constructs...
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Báo cáo khoa học: Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors doc
Ngày tải lên :
16/03/2014, 11:20
... as the conantokins, the c-conotoxins, the I-conotoxins, and the e-conotoxins [17], and in the N-terminal region of the a4 ⁄ 7-conotoxin GID [18] However, Vc1a and peptides SrIA and SrIB are the ... the a1b1cd receptor, as shown in Fig 4A, and also for the a4b2 and a3b4 receptors [12–14] The pretreatment time and the concentration of each toxin were varied in the range 3–150 s and 0.2 nm ... determination and cloning of the cDNA encoding two novel peptides, SrIA and SrIB, found in the venom of Conus spurius The pattern and the spacing of their cysteines indicate that they belong to the a-Conotoxins...
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Báo cáo Y học: Refolding of the Escherichia coli expressed extracellular domain of a7 nicotinic acetylcholine receptor Cys116 mutation diminishes aggregation and stabilizes the b structure pdf
Ngày tải lên :
31/03/2014, 23:20
... promising for further physicochemical studies On the other hand, the chromatography on Superdex 200 in the absence of 0.1% SDS revealed a dramatic difference between the GST–a7(1–208) protein and its ... were shown by Green and coworkers to depend on the state of cysteine residues in the ECDs of the a7 and a subunits, respectively [26,32] The major role of the redox state of the disulfide Cys128–Cys142 ... better than the starting protein (Table 1), and its CD curves are not very sensitive to the conditions of refolding or measuring the spectra Therefore, the conformation of the a7 moiety in the mutant...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
Ngày tải lên :
07/03/2014, 11:20
... (aN–a1), the aF–aG region and the C-terminal region (a4–a5) were well conserved between GOA-1 and Gao [31,32] The five regions of the Ga-subunit involved in receptor recognition are the a2 helix, the ... b6 ⁄ a5 loop, the a5 helix and the N- and C- extreme termini [31] In addition, the a4 helix and a4 ⁄ b6 loop region of Gai1 are important for specific recognition of receptors [33] The distinct ... suggesting that the use of M2 is restricted by the influence of intrinsic acetylcholine In C elegans, three muscarinic acetylcholine receptors have been found (GAR-1, -2 and -3) Although the pharmacological...
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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
Ngày tải lên :
30/03/2014, 10:20
... at the interface between one a subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4] The c ⁄ e and d subunits play a major role in shaping the ligand-binding ... muscle nicotinic acetylcholine receptor Neuron 3, 349–357 Sine S & Claudio T (1991) Gamma- and delta-subunits regulate the affinity and the cooperativity of ligand binding to the acetylcholine receptor ... [13] These results suggest that the acetylcholine- binding proteins and the b ECD have similar structures and that the secondary structures of a non-a ECD (b) and the a ECD resemble one another,...
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báo cáo hóa học: " Activation of α7 nicotinic acetylcholine receptor by nicotine selectively up-regulates cyclooxygenase-2 and prostaglandin E2 in rat microglial cultures" pdf
Ngày tải lên :
19/06/2014, 22:20
... performed the ELISA and the immunofluorescence staining, was primarily responsible of the RT-PCR review the data and drafted the manuscript MAAC participated in the design and coordination of the study, ... epithelial and endothelial cells express alpha7 nicotinic acetylcholine receptors Mol Pharmacol 2001, 60:1201-1209 Matsunaga K, Klein TW, Friedman H, Yamamoto Y: Involvement of nicotinic acetylcholine ... production of the primary microglial cultures and in RT-PCR LM contributed to the design of the study, guided data interpretation and presentation and assisted in the preparation of the manuscript...
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Báo cáo y học: " TTF-1 regulates a5 nicotinic acetylcholine receptor (nAChR) subunits in proximal and distal lung epithelium" doc
Ngày tải lên :
12/08/2014, 13:22
... proposed linking the two in the lung to date Neuronal and non-neuronal nicotinic acetylcholine receptors (nAChRs) combine with glycine, GABAA, and 5HT3 receptors to form a family of ligand-gated ion ... joining a5 and a7 in conserved regulatory pathways Furthermore, because comparisons between the human a5 gene and the a5 gene in several other species reveal remarkable conservation, TTF-1 and its ... reduced branching morphogenesis and severe lung hypoplasia Discussion and Conclusions The temporal-spatial distribution of a5, a member of the nicotinic acetylcholine receptor subunit family, was...
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Báo cáo y học: " Immunohistochemical detection and regulation of a5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis" pps
Ngày tải lên :
12/08/2014, 13:22
... Grando SA: Nicotine Enhances Expression of the Alpha 3, Alpha 4, Alpha 5, and Alpha Nicotinic Receptors Modulating Calcium Metabolism and Regulating Adhesion and Motility of Respiratory Epithelial ... Human Bronchial Epithelial and Endothelial Cells Express alpha7 Nicotinic Acetylcholine Receptors Mol Pharacol 2001, 60.6:1201-9 27 Lazzaro D, Price M, de Felice M, Di Lauro R: The Transcription ... A, Russo P: The Cholinergic System and Cancer Semin Cancer Biol 2008, 18.3:211-7 Rogers SW, Mandelzys A, Deneris ES, Cooper E, Heinemann S: The expression of nicotinic acetylcholine receptors by...
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Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt
Ngày tải lên :
19/02/2014, 12:20
... the residue type and in the case of loop in both the type and number of residues The N- and C-termini are labeled (B) On the left is the three-dimensional structure of the 4/6 framework a-conotoxin, ... format with the N- and C-termini labeled On the right is the corresponding schematic representation of the conserved residues in the 4/6 a-conotoxin sequences (C) On the left is the three-dimensional ... activity at the neuronal nAChR, i.e D5, P6, R7 and W10, are shown in pink The N- and C-termini are labeled The two views are rotated by 180° about the y-axis (B) The surface diagram of the three-dimensional...
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Báo cáo khoa học: a-Conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californicaand some neuronal subtypes of nicotinic acetylcholine receptors pdf
Ngày tải lên :
16/03/2014, 13:20
... indicated a-conotoxins and their analogs Final concentrations of the radioligand and toxin-binding sites of receptor were 280 and 230 nM, respectively The data shown are the averages of two independent ... forms of these peptides, and 125I-labeled GI was used as a tracer to test the SI and SIA analogs In these experiments the synthetic a-conotoxins GI, SI, SIA and MI were used as controls The respective ... before treatment with the a-conotoxin The points are either the mean ± SE from to experiments or the mean from duplicates The curves were fitted to the Hill equation The IC50 and Hill coefficient...
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Nicotinic acetylcholine receptors potx
Ngày tải lên :
29/07/2014, 00:20
... muscle-type nicotinic receptors and neuronal-type nicotinic receptors In the muscle-type receptors, found at the neuromuscular junction, receptors are either the embryonic form, composed of α1, β1, δ, and ... in the hydrophobic regions Binding the channel For ligands, see Nicotinic agonist and Nicotinic antagonist As with all ligand-gated ion channels, opening of the nAChR channel pore requires the ... to the molecules that bind receptors, such as ligand As well as the endogenous agonist acetylcholine, agonists of the nAChR are nicotine, epibatidine, and choline In neuronal nAChRs, the acetylcholine...
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Báo cáo y học: "Genetic polymorphism of ACE and the angiotensin II type1 receptor genes in children with chronic kidney disease" pps
Ngày tải lên :
11/08/2014, 03:20
... progression of the majority of chronic nephropathies [13,14] Among the candidate genes of the RAS, the ACE, and angiotensin II type receptor (AT1RA1166C) genes seem to be particularly biologically and clinically ... for hypertension and cardiovascular (CVD) disease [21] The aim of the present study was to investigate the association between polymorphisms of the ACE and AT1RA1166C genes and the occurrence of ... in the serum catalyzes the hydrolysis of the FAGG; forming furyl acryloyl phenylalanine (FAP) The decrease of the absorbance in the unit time at 340 nm is proportional to the activity of the...
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Báo cáo y học: " Genetic polymorphism of ACE and the angiotensin II type1 receptor genes in children with chronic kidney disease" pot
Ngày tải lên :
11/08/2014, 06:23
... progression of the majority of chronic nephropathies [13,14] Among the candidate genes of the RAS, the ACE, and angiotensin II type receptor (AT1RA1166C) genes seem to be particularly biologically and clinically ... for hypertension and cardiovascular (CVD) disease [21] The aim of the present study was to investigate the association between polymorphisms of the ACE and AT1RA1166C genes and the occurrence of ... in the serum catalyzes the hydrolysis of the FAGG; forming furyl acryloyl phenylalanine (FAP) The decrease of the absorbance in the unit time at 340 nm is proportional to the activity of the...
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O NEUROTOXINS NOVEL ANTAGONISTS OF NICOTINIC ACETYLCHOLINE RECEPTORS FROM SNAKE VENOM
Ngày tải lên :
30/10/2015, 17:10
... helpful in the isolation of nicotinic acetylcholine receptors (nAChRs) and understanding of their role in neurotransmission Since then, a number of new ligands for nAChRs and other receptors have ... targeting various receptors (nicotinic acetylcholine receptors, biogenic amine receptors and integrin receptors), enzymes (acetylcholine esterase and blood coagulation factors) and ion-channels ... Cys26-Cys30 for recognition of the α7 receptor and Lys23 and Lys49 for the Torpedo receptor In addition, the cyclic structure formed by the fifth disulphide bridge at the tip of the second loop of α-cobratoxin...
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Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx
Ngày tải lên :
23/03/2014, 05:22
... nm and 52 ± 14 nm, respectively (Fig 4) Both MT1 and MT2 are reported to bind efficiently to the M1 receptor and less effectively to the M3 [21] and M4 receptors [22] MT3 is a selective ligand ... M4 and M5 receptors, demonstrating not only that the toxin changes the receptor affinity for ACh, but also that the activity of the ternary WTX–ACh receptor complex is different from that of the ... concentrations were 200 pm (for M1, M3 and M5 receptors) or 500 pm (for M2 and M4 receptors), in the presence of lm (M1, M3 and M5 receptors) or 50 lm (M2 and M4 receptors) of GDP Nonspecific binding...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
Ngày tải lên :
19/02/2014, 16:20
... HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position ... of Prm3 The following lists the identities of the Prm3 gene fragments and corresponding plasmids generated in either pGL3Basic (pGL3b) or pGL3Enhancer (pGL3e) vectors and the identity of the specific ... expression and hence may shed further insights as to the physiologic requirement for two TP receptors, namely TPa and TPb, in humans Results Functional analysis of promoter of the human TXA2 receptor...
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Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt
Ngày tải lên :
07/03/2014, 03:20
... that the hh dimers, but not the tt dimers, represent the physiological structures On the other hand, it has been proposed that the hh dimer and tt dimer structures both occur, and represent the ... inactive and the hormone-activated states of the receptor, respectively [13,14] It is hypothesized that a hormone-induced rearrangement of the ECD from the hh to the tt dimer structure brings the ... function (CTF), and only data within the first zero of the CTF were used On the basis of the defocus series, this effectively limited the resolution of ˚ the reconstruction to 22 A Therefore, the reconstruction...
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