saco chip pet chip seq methods and characterization of data sets

... permittivity and the thickness of the oxide layer, and the functions of 34 Chapter Modeling of On -Chip Single Interconnect εreq, CBOT, CVP, and CTOP were given in Table I and II in [44] Csi and Rsi ... wideband series impedances and shunt conductances, conductive and dielectric attenuation constants of even (c) and odd (π)- modes, pulse waveform distortion and crosstalk, and average power handling ... determination of wideband series impedances and shunt conductances, conductive and dielectric attenuation constants of even (c) and odd (π) modes, pulse waveform distortion and crosstalk, and average...

Ngày tải lên: 12/09/2015, 08:20

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...

Ngày tải lên: 05/09/2013, 09:08

... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117 ... compartment (mol.L-1) VB is the volume of right reservoir (cm3) A and L are the membrane cross-sectional area and thickness, respectively α is the slope of the linear plot of methanol concentration in...

Ngày tải lên: 05/09/2013, 16:11

... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... μS/cm) having inlet temperature of 27◦ C Based on the length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections...

Ngày tải lên: 22/12/2013, 08:58

... evaporation of solvents and volatile products, took place simultaneously with the deposition process, caused the porosity of the TiO2 films Prepareation of TiO2/SnO2: After the material characterization ... concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different period of time Photoconductivity measurement In order to evaluate properties of obtained ... resistance (RD) and the light resistance (RL) under the irradiation of 7W Hg lamp at the distance of 10cm were measured The calculated ratio of RD/RL was considered as a photoconductivity of obtained...

Ngày tải lên: 13/02/2014, 04:20

... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other ... expression of t-bet, stat6 and foxp3 was observed in the spleen, kidney, gill, gut, liver and skin tissue of healthy fish (data not shown) After stimulation of kidney cells with a variety of immunostimulants, ... and foxp3 gene organization and chromosome synteny Using the zebrafish t-bet, stat6 and foxp3 cDNA sequences elucidated by PCR and the regions of the 132 zebrafish genome that contained these sequences,...

Ngày tải lên: 16/02/2014, 09:20

... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants ... http://www.phytozome.net/soybean#A) Comparison of the genomic sequences of GmPDIL-3a and GmPDIL-3b with those of the Arabidopsis and rice orthologs showed conservation of exon ⁄ intron structure across these ... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b...

Ngày tải lên: 18/02/2014, 11:20

... vivo excised and sequenced 4088 Sequence analysis and phylogenetic tree construction A phylogenetic tree was constructed using deduced sequences of MH1 and MH2 domains, respectively The sequences ... indicated at the bottom of each schematic representation of the gene, cDNA, and protein, respectively The schematic representation and the amino acid sequence of SmSmad1B protein show sequence motifs ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of...

Ngày tải lên: 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... hemagglutination and toxicity toward mice (data not shown), additional efforts to cleanly isolate the isoform were not successful and further characterization was abandoned A chromatofocusing step ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...

Ngày tải lên: 18/02/2014, 16:20

... MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment ions Subsequently, for specifying the C-terminus of the ... the N-terminus and C-terminus of the protein, many other peptides were also analyzed, and the results confirmed most of the remaining deduced amino acid sequence Edman sequencing of purified tryptic ... peptides covered 39.1% of the sequence The molecular masses of the tryptic peptides, either from the mass fingerprint or purified peptides, covered 60.3% of the sequence, and the masses of cyanogen bromide...

Ngày tải lên: 19/02/2014, 06:20

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... construction of plasmid pET2 8a-PsCBL whose sequence was verified before being used for protein expression Expression and purification of CIPK and PsCBL proteins The plasmids pET2 8a-PsCIPK and pET2 8a-PsCBL...

Ngày tải lên: 19/02/2014, 07:20

... determined to be 2083 bp and 2142 bp, respectively Clones C1 and C2 were Fig Comparison of the predicted amino acid sequences of rainbow trout (rt) HSF1a and HSF1b with the sequences of zebrafish (z), ... ENDGAPS, off; NOPGAPS, off; NOHGAPS, off Graphical output of the bootstrap figure was produced by the program TREEVIEW Isolation of RNA and RT-PCR analysis Total RNA was isolated from RTG-2 cells and ... initiation cycle of 15 at 95 °C, amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat...

Ngày tải lên: 19/02/2014, 12:20

... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization products was performed using a variety of ... (moles/L) · volume of titrant (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent...

Ngày tải lên: 19/02/2014, 12:20

... membrane and used for sequencing with an automated protein sequencer (Model 492, Applied Biosystems, Foster City, CA, USA) 5362 K Takahara et al For analysis of MALDI-TOF MS data 10 lL of purified ... activities of AOD and GAT and accumulation of DRIP-1 protein during the progressing drought ⁄ strong-light stress in wild watermelon leaves (A) Changes in AOD (j) and GAT (h) activity Data points ... clone of the GAT, genespecific primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE The cloned cDNA encoded a protein 5356 K Takahara et al composed of...

Ngày tải lên: 20/02/2014, 03:20

... potential, and addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA ... antibiotics: relationship of DNA alkylation and sequence specificity to the biological activity of natural and synthetic compounds Chem Res Toxicol 1, 258–268 27 Petrusek, R.L., Anderson, G.L., Garner, ... the vicinity of mtDNA with the resultant binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted...

Ngày tải lên: 20/02/2014, 11:20

... Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron ... range of PHR/PHO ratios (up to 6), keeping fixed a PHO concentration of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 ... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and...

Ngày tải lên: 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... partial spectroscopic and redox characterization of the NrfH subunit is also presented Up to now, there has been no information of this kind on any NrfH protein Materials and methods Protein purification...

Ngày tải lên: 21/02/2014, 00:20

... O-acetylsialic acid and agglutination ability of the De-O-acetylated preparation of glycoproteins De-Oacetylation of glycoproteins was performed following the procedures of Sarris and Palade [30] and Schauer ... [30] and Schauer [6] A solution of 750 lL of glycoprotein (5 mgÆmL)1) was added to 250 lL of 0.04 M of NaOH, vortexed, incubated on ice for 45 and neutralized with mL of 0.01 M HCl, respectively ... microtiter well and mixed The values of the HAI titer were determined after 60 of incubation and expressed as the highest dilution of sugars that inhibited the agglutination of erythrocytes Mannose,...

Ngày tải lên: 21/02/2014, 00:20

... Nucleotide sequencing Nucleotide sequencing was performed on clones of the pCRÒII TOPO (Invitrogen) and pET- 24a(+) (Novagen) vectors carrying the inserts T7 and Sp6 primers were used for both sense and ... Fluka ¨ Chemie AG, Switzerland Phenylmethanesulfonyl fluoride and okadaic acid were from Sigma Trypsin of modified sequencing grade was obtained from Promega, and LysC of Achromobacter lytii from ... with the Fig Nucleotide sequence of cloned cDNA from human embryonic kidney cells, and the corresponding amino-acid sequence of the recombinant protein The sequence data have been submitted to GenBank...

Ngày tải lên: 21/02/2014, 01:21

... catalase activity, oxidase activity and OF test, were determined using classical methods [15] Alkali production of amides, organic acids, reduction of tetrazolium, and requirement for nicotinamide ... 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately Effect of various compounds on the enzyme activity The effect of metal salts, and chelating and sulfhydryl ... ± 0.10 lmol of 4-amino-3-hydroxybenzoic acid and 0.90 ± 0.08 lmol of O2 were consumed and 1.1 ± 0.02 lmol of 2,5-pyridinedicarboxylic acid was formed, which indicated a molar ratio of 4-amino-3-hydroxybenzoic...

Ngày tải lên: 21/02/2014, 01:21