rss activity of viruses
Báo cáo sinh học: " Polymerase activity of hybrid ribonucleoprotein complexes generated from reassortment between 2009 pandemic H1N1 and seasonal H3N2 influenza A viruses" ppt
Ngày tải lên :
18/06/2014, 18:20
... primary site of replication of influenza viruses Our results showed that the polymerase activity of 2009 pdmH1N1 vRNP was significantly lower than WSN H1N1 and H3N2 The difference in activity was ... 2009 pdmH1N1 and H3N2 viruses could still retain a substantial level of polymerase activity Substituion of the subunit PA confers the most prominent effect on polymerase activity Further studies ... of the 2009 pandemic H1N1 and seasonal H3N2 viruses, coinfection of these viruses in humans may occur [32] In this study, the polymerase activity of recombinant vRNP complexes that may be created...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
Ngày tải lên :
25/10/2012, 11:15
... progression in eight different types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombinant His6-tagged PR ... was performed using GraphPad InStat (GraphPad Software, San Diego, California, USA) Anticancer activity of PR domain by cDNA transfection The cloning of the PR domain (residues 13-193) has been ... respectively Western blot of PR domain The expression of the PR domain was examined after 24 hr of transfection with pcDNA/HisMax/PR and the empty vector, respectively Supernatants (30 g) of the cell lysates...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
Ngày tải lên :
05/09/2013, 08:40
... organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of ... only net chemical profiles, and the spatial resolution is also above a single-cell level To address the question of the higher abundance and activity of SRB in oxic zones of biofilms (Okabe et al., ... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly...
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Photocatalytic bactericidal activity of silver-sensitized titanium dioxide on Micrococcus lylae
Ngày tải lên :
05/09/2013, 09:08
... complexity of cell wall Silver ion (Ag+) is a bactericidal agent (Pedahzur et al., 1995) The binding of Ag+ and respiratory enzymes initiates changes in permeability of plasma membranes of bacteria ... standard deviation of independent triplicates - 49 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Effect of Ag content in photocatalysts Bactericidal activities of the three photocatalysts ... deviation of independent triplicates - 50 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Increasing the concentration of photocatalysts did not increase the bactericidal activity...
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Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf
Ngày tải lên :
14/02/2014, 14:20
... structure of the available inhibitors of cathepsin D [19], a lysosomal protease of mammalian cells, and chalcones and phenothiazines have been assayed as inhibitors of falcipain-2 [20,21] Inhibition of ... Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of proteases of T gondii [14] and HIV [12,31] To ascertain the inhibition range of MA on the different ... proteolytic hydrolysis of haemoglobin as a source of amino acids constitutes one of the essential processes which take place along the intra-erythrocytic stage of the parasite Degradation of haemoglobin...
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Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt
Ngày tải lên :
16/02/2014, 09:20
... hydrolytic activity towards ATP (Fig 1A) The binding of a magnesium cofactor has long been established as essential for the activity of canonical GTP-binding proteins [29,31] The precise role of Mg2+ ... determine the requirement for magnesium of the GTPase activity of TorD, the reaction was performed in varying amounts of MgCl2 (Fig 1B) The GTPase activity of TorD without added MgCl2 is negligible, ... co-ordination of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12] The concentration of GTP in the cytoplasm of...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
Ngày tải lên :
16/02/2014, 09:20
... activity by measuring degradation of the Ac-DEVD-pNA peptide (right panel) The graphs represent average cell survival as a percentage of the control and the average fold increase of caspase activity ... induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngÆmL)1 TRAIL for h led to a significant loss of cell viability (33.1 ± 3.3% of control; ... between the proteins BRET ratios were plotted as a function of the excited GFP activity to total rLuc activity ratio, allowing comparison of BRET ratios between Bid–GFP and tBid–GFP when expressed...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... detected in the case of mGFP–calpain 7C290S, suggesting that the degradation was caused by proteolytic activity of mGFP–calpain itself We refer to this issue later Effects of mutations of IST1 MIMs on ... interaction enhances the autolytic activity of mGFP– calpain 7–Strep in vitro Autolytic properties of mGFP–calpain 7–Strep We further characterized the autolytic activity of mGFP–calpain 7–Strep Purified ... but not by GST–MIML326D,L353A or GST Enhancement of autolytic activity of mGFP– calpain by calpain 7–IST1 interaction As shown in Fig 3, expression of mGFP–calpain in HEK293T cells generated 45...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
Ngày tải lên :
18/02/2014, 11:20
... presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74 Surprisingly, the Y74W mutant exhibited loss of both activity ... the progress of the reactions of WT enzyme and other mutants is very fast The results are summarized in Fig It is evident that the Y74W* mutant shows an enhancement of activity of 21.9-fold over ... the geometry of the active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of mutational...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... understanding of the generic principle of catalysis for this family of proteases Results and Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ... 0.285 0.218 0.134 0.725 0.022 0.013 0.002 0.364 0.241 No activity Very low activity 0.383 0.003 Very low activity No activity No activity No activity 123.74 0.062 A kcat ⁄ Km (s)1ÆmM)1) Km (mM) 5.0 ... other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig 1) Substrate specificity and activity of ervatamins...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
Ngày tải lên :
18/02/2014, 16:20
... chaperone activity of aB-crystallin [27], which was reported to occur via enhancement of the dynamics of subunit assembly [28] However, to date there have been no reports of the effects of such ... Results The effect of Arg-HCl on the chaperone activity of aB-crystallin is target protein-specific In order to investigate the effect of Arg-HCl on the chaperone activity of aB-crystallin, we ... ability of aB-crystallin in the presence of that concentration of the additive amount of DTT-induced aggregation of a-lactalbumin was increased in a concentration-dependent manner by the addition of...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
Ngày tải lên :
19/02/2014, 06:20
... these data are suggestive of a role of membranes, in particular of negatively charged membranes, in the mechanism of action of RNases, we investigated the ability of a series of RNases to destabilize ... shows the shape of the electrostatic field of BS-RNase seen from different directions Figure S3 compares the electrostatic field of different Fig Plot of the EIE values as function of rotation (r) ... cycles of steepest descent followed by 50 cycles of conjugate gradients and 50 cycles of steepest descent) No clashes were detected at the dimer interface of the models or in the surroundings of...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
Ngày tải lên :
19/02/2014, 07:20
... lM of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 mM of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... strand exchange activity of OsDmc1 by FRET Strand exchange activity of OsDmc1 Protein concentration dependence Fig Renaturation activity mediated by OsDmc1 protein in the presence of ATP and slowly ... Renaturation reaction of OsDmc1 (1) without competitor; (2) with 27.5 lM unlabeled of PhiC along with 27.5 lM of rhodamine labeled Phi-C; (3) with 55.0 lM unlabeled of Phi-C along with 27.5 lM of rhodamine...
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Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
Ngày tải lên :
19/02/2014, 07:20
... of increasing concentrations of native and oxidized elafin and trappin on the activity of a constant concentration of NE and kdiss Ki ¼ kdiss ⁄ kass [5,8] Trappin is a 9.9-kDa protein formed of ... prevents easy binding of the methionine sulfoxide residue at the S1¢ subsite of the active centers of NE and Pr3 The fact that the S1¢ subsite of Pr3 is significantly smaller than that of NE [21] might ... Calculation of kass for the NE-oxidized elafin and trappin complexes using the measured values of Ki and kdiss shows that oxidation also decreases the rate constant of enzyme inhibition by factors of three...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
Ngày tải lên :
19/02/2014, 12:20
... in Table Assay of the N-glycosidase activity of ricin A chain variants The activity of each of the RTA variants was determined by assessing their ability to depurinate 26S rRNA of Table Data collection ... effect of substitutions made at each of the arginyl residues on catalytic activity of RTA, the ability of each of the purified RTA variants to depurinate yeast ribosomes was compared to that of wildtype ... the catalytic activity of RTA, the N-glycosidase activity of RTA N122A against yeast ribosomes was determined and compared to that of wild-type RTA (Fig 6A) The reduction in activity of this RTA...
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
Ngày tải lên :
19/02/2014, 12:20
... encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and reconstitution of the enzyme activity by co-expression of recombinant CHL D, CHL H and ... with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation This would also lead to a release of the blockade of the ATP-binding site of BchI by the integrin I domain, ... (numbered according to [24]) Plasmid pAntG1 has an insert of 717 bp of genomic Xantha-g DNA and produces 54 residues of the C-terminal half of the XAN-G protein The XAN-G specific residues AVRVGLNAEKSGDVG...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... constant of most of the amide protons was in the range of 6–8 Hz, suggestive of rapidly interconverting conformers that coexist in solution The temperature coefficients of chemical shift of amides ... topologies of the turn region mimic those of rat CD2 Structure of cyclic hexapeptides – cEL peptide The chemical shifts of amide resonances of peptide cEL were well dispersed over a region of 1.2...
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Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
Ngày tải lên :
19/02/2014, 16:20
... optimized Results Effects of truncation of the cytoplasmic H4-H5 loop of the a subunit of Na+/K+ ATPase on TNP-ATP binding and p-nitrophenylphosphatase activity Molecular modeling of the H4-H5 loop according ... observed neither a change of the excitation nor of the emission uorescence spectra in the presence of any of these H4-H5 loopGST ể FEBS 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur ... bound per H4-H5 loop or per the a subunit of Na+/K+ ATPase [17] K+ activated phosphatase activity of the ATPase as well as the phosphatase activity of the H4-H5 loop protein were tested as described...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
Ngày tải lên :
19/02/2014, 17:20
... the transport activity were normalized for the surface protein of these mutants Double N-glycosylation mutants showed a marked reduction of GABA-uptake activity of 60–40% of that of the wild type ... N-Glycosylation mutations result in reduction of GABA-uptake activity For quantitative measurement of the specific activity of GABA-uptake, an aliquot of the stable CHO transfectants was used for ... reduced stability of the N-glycosylation mutants of GAT1 must be a result of misfolding of these proteins The deficiency of N-glycosylation results in a markedly reduced GABA-uptake activity (Fig...
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