role of copper in blood cell formation and function

... pyrocarbonate DISC death inducing signalling complex xv DIABLO direct inhibitor of apoptosis protein-binding protein of low isoelectric point Diva death inducer binding to vBcl-2 and Apaf-1 DMEM Dulbecco’s ... adhesion molecule IFN-γ interferon gamma IL-1β interleukin-1 beta IL-4 interleukin-4 IL-6 interleukin-6 IL-8 interleukin-8 IL-10 interleukin-10 xvi IL-13 interleukin-13 iNOS inducible nitric oxide ... lesions such as controlling of the BBB (Blood Brain Barrier) and invasion of haematogenous cells as well as removing and downregulating pro-inflammatory cytokines (Streit and Kreutzberg, 1988)...

Ngày tải lên: 12/09/2015, 08:19

... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... control cells, but was more evenly distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and ... perinuclear foci In contrast, while overexpression of Trip10 in CP70 cells also increased huntingtin levels, both proteins remained in the cytosol without apparent foci formation Western blot and...

Ngày tải lên: 10/08/2014, 05:21

... conception and design, or acquisition of data, or analysis and interpretation of data; 2) have been involved in drafting the manuscript or revising it critically for important intellectual content; and ... survival and overall survival in the subgroup of patients receiving thoracic irradiation and an improved brain metastasis free survival and overall survival in the subgroup of patients receiving prophylactic ... management of small cell lung cancer up to the present Surgery Revived The role of surgery was revaluated after the introduction of the TNM staging system The clinical trials that appeared in the...

Ngày tải lên: 10/08/2014, 10:20

... tobacco smoke (in utero and at time of survey) A Botto Khoury approach summarizing the data in a × table allowed for the evaluation of the independent and combined roles of genotype and exposure ... baseline spirometry using increasing concentrations of histamine (usually from 0.5 mg/ml to 8.0 mg/ml) according to standard procedures The 90th percentile of the distribution of PC20FEV1 in a ... because of different techniques of BHR assessment in the case-control study and in the cohort study analysis in the cohort study because of low number of cases while FEV1 was increased in carriers of...

Ngày tải lên: 12/08/2014, 16:20

... Results 3.1 Investigating the roles of the BCH domain in novel proteins 3.1.1 In silico identification of a novel BCH-domain containing 118 118 protein 3.1.2 Sequence verification and bioinformatics ... and cell lines 133 3.2.1.2 Expression profile of BNIPXL in murine tissues and cell lines 134 3.2.2 Domain architecture of BNIPXL constructs 140 3.2.3 BNIPXL contains a functional protein-protein ... increasing number of Dbl homology (DH) domain-containing proteins were isolated In addition to the DH domain, these proteins also possessed a pleckstrin homology (PH) domain found in tandem and...

Ngày tải lên: 16/09/2015, 08:31

... epithelial (NHBE) cells Materials and methods Maintenance of human epithelial cell lines Cells from a human bronchial epithelial cell line (Calu-3) and from a human alveolar epithelial cell line (A549) ... cell suspension containing 80– 95% of lymphocytes and 5–20% monocytes The cells were resuspended in cell media with 1% FCS to million white blood cells/ml and plated in 48 well cell culture polystyrene ... increased amounts of leucocytes in lung tissue in COPD patients interact with several cell types including lung epithelial cells as well as endothelial cells in a similar manner increasing IP-10 secretion...

Ngày tải lên: 12/08/2014, 15:21

... 35 Induction of micronuclei correlates with a decrease in cell viability and increase in cell death in MCF7 cells 127 36 Induction of micronuclei correlates with a decrease in cell viability and ... transformed cell lines 3.2.4 Doxorubicin retards proliferation in both transformed and 120 non-transformed cell lines 3.2.5 Doxorubicin induces micronuclei formation in transformed 124 cell lines in a ... structure consisting of the C-terminal catalytic domain linked to the N-terminal regulatory domain through a lysine- and glycine-rich (KG) linker domain The C-terminal catalytic domain is highly...

Ngày tải lên: 14/09/2015, 14:13

... acquisition and interpretation GI was involved in data acquisition and interpretation, and manuscript drafting SB and AB were involved in data acquisition AF was involved in data interpretation and manuscript ... evidence that blood lactate levels during the ICU stay are initially higher in pediatric patients receiving a bloodless priming solution than in those receiving a blood- containing priming solution ... cardiac operation using CPB during the study period in whom RBCs were used in the priming solution of the CPB circuit were included in the bloodprime group The use of RBCs in the priming solution is...

Ngày tải lên: 13/08/2014, 20:21

... accumulation and eventually cause cellular necrosis and leakage of copper into the plasma and deposition of copper in extra hepatic tissues, including the limbus of the cornea and the basal ganglia of ... contains a leading and a lagging strand Lagging strand synthesis cannot be completed because the removal of primers thus causing net loss of DNA base pairs from the lagging strand (Hug and Lingner, ... and Cellular biology – Stem cells, cancer and aging Singapore • Balakrishnan L, Gurung RL, Hande MP (2007) Effect of Combined inhibition of PARP-1 and telomerase 5th Anniversary Congress of International...

Ngày tải lên: 11/09/2015, 10:18

... Human SPHK Inhibitors Over-expression of SPHK1 and SPHK2 was achieved by transfecting the SPHK1 or SPHK2 plasmids into CHO cells using Lipofectamine TM 2000 (Invitrogen), following the instructions ... developed inhibitors, a better understanding of the role of SPHK in the process of stem cells differentiation will be studied as well In this chapter, only the compounds synthesis and evaluation ... Cell lysis CHO cells were collected and re-suspended in suitable amount of SPHK buffer (described below) Cells were lysed by several cycles of freeze-thawing and total cell lysates were obtained...

Ngày tải lên: 11/09/2015, 16:06

... Penicillin and Streptomycin DMEM+10% FBS +1% Penicillin and Streptomycin Supplements N/A 0.5mM isobutyl-methylxanthine, 1μM dexamethasone, 10μM insulin, 200μM indomethacin, 1% Penicillin and Streptomycin ... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... 3.2.3.1.2.2 Immunostaining of Osteocalcin, Osteopontin, and osteonectin The expression of osteocalcin, osteopontin, and osteonectin was measured by immunostaining after 28 days of culture with or without...

Ngày tải lên: 11/09/2015, 16:06

... signaling pathways; and part of it will penetrate into the cells and directly act as an intracellular second messenger Both functions of S1P contributed to the intracellular calcium release, and ... cultured in 6-well plates were washed twice with 1x PBS and starved in serum-free DMEM for half an hour 1μM S1P was then added into the wells and incubated at 37°C for 5min, 10min, 30min, and 60min ... 10 cell growth 120% culture conditions 131 Chapter Role of S1P in human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM-and...

Ngày tải lên: 11/09/2015, 16:06

... the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... Conclusion and Future Work being one of the most potent and specific SPHK1 inhibitors (chapter 2), DMS was continuously used as a reference inhibitor It is interesting to note that CP6 and DMS affected ... for SPHK inhibitors in adult-stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human BM- and AD- MSCs...

Ngày tải lên: 11/09/2015, 16:06

... (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” Biochemistry, ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, it could be secreted and function ... the roles of SPHK and S1P in stem cell proliferation, stemness maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness ... involved in various cellular events in physiological and pathophysiological processes Activation of SPHK and accumulation of S1P are believed to be involved in cell proliferation, including in...

Ngày tải lên: 11/09/2015, 16:06

... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in I ... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... embryonic cells (Martin and Evans, 1975) EC cells could be expanded continuously in culture while retaining the capacity to differentiate into derivatives of all three germ layers (Kleinsmith &...

Ngày tải lên: 12/09/2015, 08:16

... kept inactive by tyrosine phosphorylation at its C-terminal Y527 which results in intramolecular binding to its own SH2 domain and hence maintaining the kinase in a closed or inactive conformation ... established inhibitory role in T cells and macrophasges, the inhibitory role of Csk in B cells and mast cells has been shown to be minimal (Hata et 32 al., 1994) A possible explaination is that in B and ... Ras-guanine nucleotide releasing protein Src family kinases Src-homology Src-homology SH2 domain -containing transforming protein C SH2-containing inositol 5’-phosphatase-1 SH2-domain containing...

Ngày tải lên: 12/09/2015, 08:16

... function of LPXN and could be critical for mediating the inhibitory role of LPXN in repressing BCR-induced IL-2 production in A20 B cells Inhibitory Role of Leupaxin in BCR Signaling 27190 JOURNAL OF ... A20 B cells and that the inhibition of cytokine secretion by leupaxin required the phosphorylation of Tyr72 Thus, leupaxin plays an inhibitory role in BCR signaling and B cell function Inhibitory ... cross-linking in B cells, suggesting that LPXN could play a role in BCR signaling Leupaxin Interacts with Lyn during BCR Signaling—Previous studies indicated that members of the paxillin superfamily...

Ngày tải lên: 12/09/2015, 08:16

... transgenic line for analyses of endocrine pancreas development and cell lineage Analyses of exocrine and endocrine pancreas cell lineage by DTA-mediated cell ablation under exocrine and endocrine pancreas-specific ... observed in the other cell type In contrast, in pp cell ablation, there was obvious β- and δ -cell reduction They proposed that pp cells may be the progenitor cells of β- and δ-cells and α- and β-cells ... a tentative lineage map of pp, β-, α- and δ-cells in mice is as follows: pp cells are indispensable for β- and δ-cells; α- and β-cells are independent of each other; α -and β/δ lineage are separate...

Ngày tải lên: 12/09/2015, 08:16

... RT-PCR showing induction of p73β in Soas2 cells resulting in upregulation of bim mRNA levels 123 Fig 3.42: p73 bind to p73RE on bim intron 125 Fig 3.43: Cell cycle dependent binding of p73 to ... during mitosis, thus allowing chromatids to separate during anaphase towards opposing poles In the cells, the spindle checkpoint is kept active through the binding of inhibitory proteins BubR1 and ... with each other (Irwin and Kaelin, Jr., 2001) Each of them consists of a N-terminus trans-activation domain, a central DNA binding domain and a C-terminus regulatory domain (Illustration 1) All...

Ngày tải lên: 13/09/2015, 21:21