resistance of tumor cells to freezing and thawing

... antiapoptotic function of HSPs was shown for monoblastoid U937 cells and murine fibrosarcoma WEHI-S cells treated with actinomycin-D, camptothecin and etoposide [15] as well as rat brain tumor cells ... mouse and human melanoma cells overexpressing hHSF1 to study the effect of HSF1 on the survival of cancer cells treated with cytotoxic agents used in chemotherapy Here, we generated melanoma cells ... Page of 11 Figure An increased resistance of melanoma cells to doxorubicin is not coupled with HSF1 transcriptional activity A Detection of transcripts of HSF1 and HSP genes in B16F10, WM793B and

Ngày tải lên: 05/11/2020, 05:25

... between ceramide production and induction of apoptosis In order to link ceramide production to the induction of apoptosis, according to the different properties of cells and radiation qualities, ... ceramide and the different types of apoptosis needs to be defined Apoptosis can occur before the first mitosis (early apoptosis) or as the last step of mitotic catastrophe [7] (late apoptosis) ... order to determine if low -and high-LET-radiation can induce apoptosis, four different techniques were used to monitor apoptosis: the quantification of sub-G1 and TUNEL positive cells, the mitochondrial

Ngày tải lên: 05/11/2020, 07:32

... contrast to the high susceptibility of hypoxic mutant NRAS MelJuso cells to 3-BrPA or Prima-1, KRAS mutant C8161 and A549 cells revealed hypoxic resistance to 3-BrPA counteracted by Prima-1 In A549 cells, ... Access Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by Nacetylcysteine Andrea Orue, Valery Chavez, Mary Strasberg-Rieber and Manuel Rieber* ... proliferation and expression of genes associated with hypoxia and/ or glycolysis in cells harbouring mutant RAS and a wt p53 gene Methods Cell Lines Human melanoma cells a) MelJuso cells are wt BRAF and

Ngày tải lên: 20/09/2020, 18:44

... properties of endothelial cells Effect of Sunitinib and Sorafenib on PTEC proliferation, cytotoxicity, tubulogenesis and migration We subsequently evaluated the effect of Sunitinib and Sorafenib, ... Combination of anti-androgen and anti-angiogenic drugs In the attempt to decrease the observed resistance of PTEC to Sorafenib, we evaluated the effect of a combined treatment with the anti-androgen ... the context of prostate cancer Additional file Additional file 1: Figure S1 Cytotoxicity resistance, proliferation and tubulogenesis of Sunitinib and Sorafenib treated PTEC and HMEC (A and B): Cell

Ngày tải lên: 30/09/2020, 13:15

... College of Natural and Computational Sciences Department of Zoological Sciences PhD thesis Abundance, Distribution and Insecticide Resistance of Anopheles Mosquitoes (Diptera: Culicidae) and Malaria ... me to Molecular Laboratory of Entomology at CDC, Atlanta, GA, USA so that I have analyzed my mosquito samples I would like to appreciate his patience in that he sent me letter of invitation and ... ways to be the man of today As a reward for your tolerance to school me 22 consecutive years, you would be feeling proud iv of having the youngest PhD holder son I would like to give the credit of

Ngày tải lên: 15/08/2017, 15:09

... total effect of a marker pair was set to p = 10-5 MapQTL® [45] was also used for mapping to include covariates and cofactors in the QTL analyses Cofactors were initially specified according to ... eV), and ABA 262.8/153.0 (8.0 eV) A calibration curve of the ratio of peak areas of the unlabelled standard to the peak area of the deuterium-labelled standard was used for the quantification of ... stre1 and stre2 alleles of Bur origin during winter High levels of SA and its glucoside were also found in the xylem sap of V longisporum-infected B napus and were correlated to the degree of stunting

Ngày tải lên: 27/05/2020, 01:37

... HUVECs were seeded into a fibronectin-coated insert and cultured for 96 h at 37 °C and 5% CO2 before 100,000 tumor cells were put on top of the monolayer The transmigration of tumor cells was quantified ... macromolecules and tumor cells was analyzed in the presence or absence of the ADAM10/17 inhibitors GI254023X and GW280264X Expression of ADAM10, ADAM17 and VE-Cadherin in endothelial cells was quantified ... adhesion molecules in both, endothelial and tumor cells, in turn leading to increased tumor cell – endothelial cell adhesion and subsequent extravasation of tumor cells, we show here that through the

Ngày tải lên: 17/06/2020, 18:42

... CCL18 Page of 14 Effect of M1 and M2 macrophages on cancer cell apoptosis In order to study the effects of M1 (pro-inflammatory and anti-tumoral) and M2 (anti-inflammatory and protumoral) THP-1 ... macrophages are cytotoxic for pathogens and tumor cells Their tumoricidal activity was related to their ability to secrete reactive nitrogen and oxygen species and pro-inflammatory cytokines [7] THP-1 ... cells M1 macrophages had a cytotoxic effect and increased the etoposide-induced apoptosis On the opposite, M2 macrophages were protective and decreased the apoptosis in cancer cells exposed to

Ngày tải lên: 28/09/2020, 10:14

... glycosylation on the binding of Herceptin to HER2 protein in breast cancer and on the sensitivity of cancer cells to the chemotherapeutic agent doxorubicin (DXR) and growth factors (EGF and IGF-1) The interaction ... following deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6.83 Hz; glycosylated cells Bmax: 7.35 Hz) The sensitivity of cells to DXR and to growth factors was evaluated using ... Cellular glycosylation affects Herceptin binding and sensitivity of breast cancer cells to doxorubicin and growth factors Diluka Peiris1, Alexander F. Spector2, Hannah Lomax-Browne3, Tayebeh Azimi3,

Ngày tải lên: 19/11/2022, 11:50

... cells and breast tumor cells To evaluate the cytotoxicity of CAP in normal breast cells (immortalized HB4a cells) and tumor breast cells (MCF-7, MDA-MB-231, and MDA-MB-436 cells) , the cell lineages ... of IL-1β and enhanced the synthesis of TNF-α in THP-1 cells (Zhang et al., 2017A) The same elevation of tumor TNF-α and reduction of tumor NAG, MPO, and NO were found in Walker-256 tumor- bearing ... inflammatory infiltration, necrosis, apoptosis, and hepatocellular degeneration The number and area of vessels of the tumor were morphometrically analyzed Images of tumor slides were captured using an

Ngày tải lên: 07/01/2023, 21:17

... combined signal from both tumor and non -tumor cells. If the proportion of tumor cells is too low, aberrations will remain undetected. Comparisons of detected aberrations in crude tumor samples with ... majority of the tumor cells. The extent of the proliferative advantage and the time between occurrence of the aberration and tumor collection influence the proportion of tumor cells with each ... or loss of whole or large parts of chromosomes. Thus the average ploidy or total genomic content of tumor cells cannot be assumed to be 2N. A fixed amount of genomic DNA is hybridized to the

Ngày tải lên: 09/08/2014, 23:20

... artificial bank and bed of the flume experiments and to take photos during the field investigation. I also thank Prof. Fujita, Prof. Ike, Prof. Viet and his staffs, JSPS, and Monbusho who ... shows bottom topography in front of the Niyodo river mouth from 1974 to 1997 From 1974 to 1988, total volume of extracted sand was more than 4.6 million cubic meters in front of Niyodo ... Estimation of Erosion Resistance of Cohesive Bank in River and around River Mouth Fig. 4.1 Sketch of test cross section of the flume 47 Fig. 4.2 Velocity profile in the center of test section

Ngày tải lên: 13/11/2014, 06:24

... for early tumor detection and disease monitoring (Jablonka and. .. tumor suppressor genes and their related genes during hepatocarcinogenesis 2 1.1 Overview of Hepatocellular ... hDAB2IP and DLEC1 in HCC and their molecular mechanisms in tumorigenesis Prior to presenting my results, I will make an overview of HCC and then review our understanding of HCC ... of oncogenes,... silences tumor suppressor genes and thus enhances the process of tumorigenesis Therefore, a greater understanding of the mechanisms underlying DNA methylation of

Ngày tải lên: 11/09/2015, 16:07

... learners of English and Vietnamese have useful and significant knowledge of PsRG&L in terms of syntactic and semantic features in English and Vietnamese. - Vietnamese learners of English and Vietnamese ... SCOPE OF THE STUDY Due to the time and resource constraints, my study is restricted to the investigation into PsRG&L in English and Vietnamese in terms of syntactic and semantic features together ... would like to use coordinators to connect members of sentences, English people would like to express simply and shortly their thoughts and ideas. 4.1.2. Syntactic Features of English and Vietnamese

Ngày tải lên: 13/09/2015, 00:17

... Scalp topography and old-new effects of low and high RW individuals of GSS1 157 Figure 17 Scalp topography and old-new effects of low and high RW individuals of GSS2... Powers, Andriks, ... intervals of interest in grouping of Total Suggestibility of collapsed GSS1 and GSS2 Figure 14 a Scalp topography of low and high DRM-free recall (RW) individuals of collapsed GSS1 and ... Thailand) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF SOCIAL WORK AND PSYCHOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2005 ii Acknowledgements Special thanks to: God:

Ngày tải lên: 15/09/2015, 17:09

... use of negative prefixes to nouns and adjectives made by the junior students in the Foreign Languages Faculty To find out the causes of the errors in the use of negative prefixes to nouns and ... advanced knowledge of English grammar According to the result of the research, the errors in the use of the prefix awith negative meaning and this prefix with the meaning of ? ?to? ?? or “towards” constitute ... distinction of the prefixes in- and im- with negative meaning and those referring to place or movement together with the mistakes related to the distinction of prefixes in-, im- with negative meaning and

Ngày tải lên: 30/11/2015, 09:12

... framework and hypotheses: The impact of risk factors to production and business performance in Vietnam 2.6.1 Conceptual framework and hypotheses: The impact of risk factors to production and business ... nine factors (one dependent factor and independent factors) shows that these factors are satisfied reliability requirement * Results from exploratory factor analysis (EFA): ● Exploratory factor analysis ... factors (one dependent factor and independent factors) indicates that these factors are satisfied reliability requirement * Results from exploratory factor analysis (EFA): ● Exploratory factor

Ngày tải lên: 10/06/2016, 10:39

... increase in apoptosis and activation of the intrinsic apoptotic pathway via trafficking of activated Bax to the mitochondria [62] Akt is known to phosphorylate Bax and to prohibit its mitochondrial ... Effect of combining epidermal growth factor receptor inhibitors and cisplatin on proliferation and apoptosis of oral squamous cell carcinoma cells Int J Oncol 30, 1469–1476 Ciardiello F & Tortora ... Stimulation of the Akt pathway inhibits Bax translocation from the cytoplasm to the mitochondria and promotes survival [45] Anti-apoptotic stimuli lead to the activation of Akt and to Ser184 phosphorylation...

Ngày tải lên: 16/02/2014, 09:20

... into the peritoneum of each mouse to obtain peritoneal cells Peritoneal cells (1 × 106 /mouse) were then analyzed by flow cytometry Characterization of tumor cell death by drug-treated tumor cells ... numbers of added Figure Flow cytometry analysis of peritoneal cells after intraperitoneal injections of MOSEC-GFP and MOSEC-dox cells MOSEC-GFP tumor cells (1 × 106/mouse) were intraperitoneally ... flow cytometery (A and B) Representative flow cytometery data of (A) TC1-GFP cells mixed with differing amounts of doxorubicin-treated TC1 cells and (B) TC1-GFP cells from the bottom well of the...

Ngày tải lên: 10/08/2014, 05:21

... times got tough Prof Jackie Ying and Ms Noreena, directors of the Institute of Bioengineering and Nanotechnology, for creating a challenging, exciting and stimulating working environment and support ... glioma cells and re-expression of miR-26b diminished proliferation, migration and invasion of glioma cells in vitro (25) Moreover, miR-26b targets ephrin A2 and diminish the capacity of glioma cells ... astrocytomas and ectopic expression of miR-335 in C6 rat glioma cells enhances its viability, soft-agar colony forming ability and invasiveness (33) Silencing of miR-335 causes growth arrest and...

Ngày tải lên: 09/09/2015, 17:53