replication proteins involved in transcription and replication

... points 23 FIGURE 2.1 DNA replication licensing control by Geminin and CDKs 42 during the cell cycle FIGURE 2.2 Geminin Binding Domain of Human Cdt1 and Cdt1 44 Binding Domain of Human Geminin ... prove useful in elucidating how Cdt1 and Geminin interact at the protein level We identified, cloned and expressed (in bacterial cells) the geminin-binding domain of human Cdt1 and purified it ... Cloning and sequencing of the Geminin Binding Domain of hCdt1 - 62 3.2 GBD-hCdt1 and RID-hGeminin were partly expressed as soluble protein 62 3.3 Protein Purification - 64 3.3.1 Affinity...

Ngày tải lên: 04/10/2015, 10:24

... were investigated Expression vectors encoding these proteins were cotransfected into HeLa cells lacking endogenous HNF1 proteins together with a luciferase reporter plasmid containing an HNF1 inducible ... chimeric HNF1 proteins, we observed an exclusively nuclear staining in transfected HeLa cells in all constructs containing the POU homeodomain (POUH) of the HNF1b protein This finding is consistent ... domains of the HNF1b protein is highly evolutionarily conserved, and is the most striking difference between the HNF1a and HNF1b proteins (Fig 1) Our transactivation Domains of HNF1b involved in...

Ngày tải lên: 23/03/2014, 13:20

... http://www.translational-medicine.com/content/7/1/75 Figure Protein Tyrosine Kinase and other Major Kinases involved in Angiogenic Pathways Protein Tyrosine Kinase and other Major Kinases involved in Angiogenic ... Serine-Threonine Protein Kinase C (PKC) and Adapter Proteins Beta type serine/threonine protein kinase C (PKC)* P05771 Protein kinase C-binding protein NELL1 (NELL1) Q92832 Annexin VI (ANXA6) ... However, the RGD domains are present in numerous integral plasma membrane proteins identified in this study including integrin and other cell adhesion proteins [82] In addition, the binding of Tat to...

Ngày tải lên: 18/06/2014, 15:20

... protein molecules and their interactions with DNA in real-time reactions and the dynamics of their DNA-binding Therefore, my objective of finding the characteristics of protein binding to single ... recognition and organize DNA into different conformations (Figure 1.8) IHF and HU bind to DNA non-cooperatively causing DNA bending (20) at low protein binding density While at high protein binding density, ... crucial role in meiosis; it synapses DNA into a stable formation in a zinc dependent manner Overall, my studies have provided important insight of four DNA binding proteins involved in several...

Ngày tải lên: 09/09/2015, 11:25

... termination but is involved in binding to poly(A)-binding protein, suggesting a link between the termination event and the initiation process in protein biosynthesis, and a conserved C-terminal ... include the poly(A)-binding protein (Pab1) and the cap-binding protein (eIF4E) Pab1 couples decapping with deadenylation and inhibits decapping by promoting the formation of the translation initiation ... decapping regulators can promote decapping efficiency The RNA-binding proteins Edc1, Edc2 and Edc3, can enhance decapping activity Edc1 and Edc2 are capable of stimulating decapping activity in...

Ngày tải lên: 14/09/2015, 22:22

... ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal ... NM_000213 NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray ... with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived cell lines The resistance against anticancer drugs...

Ngày tải lên: 31/10/2012, 15:28

... the importin b binding (IBB) domain in its N-terminus and the C-terminal NLSbinding domain In the absence of importin b, an auto-inhibiting part of the IBB domain forms an intramolecular interaction ... of basic amino acid residues such as lysine (K) and arginine (R), and (ii) the presence of conserved proline(s) (P) potentially involved in breaking secondary structural elements within the NLS ... NLS-binding domain, preventing the association with NLS on the cargo protein Thus, the presence or absence of importin b regulates the NLS binding ability of importin a The relatively large NLS-binding...

Ngày tải lên: 15/03/2014, 11:20

... d-catenin The armadillo, ARM, domains, predicted 14-3-3 binding site and a PDZ binding motif are indicated (B) d-catenin complexes in adherens junction targeting and interaction with cadherin FEBS ... together via cadherin-catenin protein complex linkages and d-catenin interacts with adhesive junction proteins both in transfected cells and mouse brain [22], Fig 1B Cadherins are Ca2+-dependent ... the insulin receptor tyrosine kinase substrate protein of 53 kDa (IRSp53) IRSp53 is an SH3 domain-containing adaptor protein originally identified as a substrate of the insulin receptor kinase...

Ngày tải lên: 16/03/2014, 22:20

... with His-Bind resin (EMD Biosciences, Inc.) After washing of the column with binding buffer containing 60 mm imidazole, recombinant proteins were eluted with binding buffer containing m imidazole, ... [31], and calreticulin, known as a chaperone for glycoprotein folding [32,33], were determined Prob-conglycinin, possessing an N-terminal prosequence, and proglycinin are transient protein forms ... storage proteins, such as glycinin [23,24] and b-conglycinin [25,26], in its protein storage vacuole (PSV), GmPDIS-1, GmPDIS-2 and BiP were localized mainly to the ER (Fig 7A–D) Interestingly,...

Ngày tải lên: 23/03/2014, 09:21

... adipophilin and perilipin genes and proteins After multiple sequence alignment of PP17b, adipophilin (hADFP) and perilipin (hPLIN), and alignment of their cDNAs to genomic sequences, aligned proteins ... PP17b PP17b is involved in apoptosis and differentiation of epithelial cells Several putative transcription factor binding sites involved in apoptosis and differentiation were localized in the PP17 ... [33] transcription factor binding sites clustered in the vicinity of the Inr might serve in transcription initiation (Fig 1) Analysis of the 1.5-kb 5¢ flanking region, attempting to get further insight...

Ngày tải lên: 23/03/2014, 21:20

... of proteins where zinc is complexed through four invariant cysteine and/ or histidine residues, forming a zinc finger domain that is mostly involved in DNA binding but also in protein–protein interactions ... present not just in transcription factors, but in basically all families of proteins involved in maintaining genomic stability, including DNA repair proteins and cell cycle control proteins [18] This ... folding of the minimal DNAbinding domain [50]; substitution of any of its zinccomplexing cysteines (Cys105, Cys108, Cys126 and Cys129) leads to diminished DNA binding and a severe reduction in...

Ngày tải lên: 30/03/2014, 15:20

... Indels were looked for in all individual transcription protein datasets Unfortunately, no indel-sharing indicative of phylogenetic relationship among groups could be found Intriguingly, the proteins ... of indels in the M kandleri transcription dataset is consistent with an accelerated evolution of transcriptional proteins in this taxon with respect to any other archaeal lineage included in ... presenting a sufficient taxonomic sampling and no evidence of multiple paralogies and/ or LGT were retained and concatenated into two large fusions, one consisting of 14 proteins of the transcription...

Ngày tải lên: 09/08/2014, 20:21

... ribonucleoprotein L; HSPD1: heat shock 60-kDa protein 1; KHSRP: KH-type splicing regulatory protein (far upstream element-binding protein 2); LMNA: lamin A/C; PCBP2: poly(rC)-binding protein 2; PRDX2: ... recognised with high intensity by IgG from SSc patients were analysed by using Pathway Studio software Interestingly, most of these proteins were involved in the transforming growth factor b (TGF-b) ... intracellular calcium-binding protein and thus is involved in signal transduction pathways In apoptotic cells, calreticulin is translocated to the cell surface, conferring immunogenicity of cell...

Ngày tải lên: 12/08/2014, 15:23

... host proteins potentially relevant in viral pathogenesis [29] Candidates for this approach may include other members of the TRIM or APOBEC families [30,31] as well as proteins involved in pathogen ... recruitment of INI1 and PML on incoming HIV preintegration complexes: interference with early steps of viral replication Mol Cell 2001, 7:1245-1254 Nisole S, Stoye JP, Saib A: TRIM family proteins: retroviral ... lentiviruses [22] Within the Vif-interaction domain of APOBEC3G, 10 residues can be pinpointed to have evolved under strong positive selection Interestingly, the APOBEC3G amino acid position 128,...

Ngày tải lên: 13/08/2014, 09:21

... annotated protein-protein interactions (PPrel), KEGG Enzymes involved in neighboring steps (ECrel) [46] and Reactome proteins involved in the same complex, indirect complex or same or indirect reaction ... are involved in transcriptional regulation, DNA binding, RNA binding, ion channel activity, ATP synthesis, actin binding and natural killer cell mediated cytotoxicity and cell proliferation Candidate ... are listed in Table These findings shed light on the pathways the candidate genes in these two regions are likely to be involved in, and may help in understanding the possible effect in T1D suggested...

Ngày tải lên: 14/08/2014, 08:20

... transmembrane protein, is activated by the ligands Delta and Jagged, which are also transmembrane proteins expressed by neighboring cells Upon ligand binding, the intracellular domain of Notch (NICD) ... of albumin and subsequently as an increase in excretion of protein, is often associated with the increased morbidity and mortality in individuals with diabetes (Mogenaen et al., 1983) In patients ... Hes proteins inhibit neuronal differentiation through two distinct mechanisms First, Hes factors have a proline residue in the middle of the basic region and exhibit a higher binding affinity...

Ngày tải lên: 30/09/2015, 06:36

... nucleosolic proteins The remaining pellet contains all DNA and structure bound proteins and is further referred to as chromatin-fraction Electrophoresis of proteins and Western blotting Cytosolic and nucleosolic ... BrdU staining and this again signifies that PCNA is only bound to chromatin portions where actively replicating DNA is present Recruitment of proteins involved in replication to chromatin during the ... according to the proteins they contain Fraction includes all ‘non-nuclear proteins , i.e cytosolic proteins separated during hypotonic preparation of nuclei, fraction contains ‘soluble nuclear proteins ...

Ngày tải lên: 21/02/2014, 00:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... binding and nuclear localization), which is shared by all FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a...

Ngày tải lên: 16/02/2014, 09:20

... Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a manner similar ... mainly expressed during larval metamorphosis, in the mantle edge and the digestive gland During the reproductive cycle, expression was high in gonads during the postspawning period but not in ... specifically expressed during mammary gland involution [23,24] Considering Cg-Clp2 patterns of expression, this protein could be involved in tissue growth and remodelling, as was formerly postulated...

Ngày tải lên: 19/02/2014, 00:20

... nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect association ... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ... members of the actin-binding LIM protein (ABLIM) family, ABLIM-2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates...

Ngày tải lên: 07/03/2014, 01:20