regulation of life span in adult stem cells

... Reversibility of the effects of calcium-induced contraction of cardiomyocytes on the kinetics of regulation of mitochondrial respiration by exogenous ADP The kinetics of respiratory regulation was ... creatine kinase reactions, including mitochondrial creatine kinase [3840] Increase of the free calcium concentration to lm resulted in some decrease of the Vmax, but its value stayed higher in ... shortening of sarcomeres hypercontraction caused by excess free calcium results in a reversible alteration of the regular arrangement of mitochondria in the cells, in the changes in the kinetics of...

Ngày tải lên: 16/03/2014, 22:20

... finding indicates the embryonic origin of teratocarcinoma stem cells In 1974, the stem cells in teratocarcinomas were successfully isolated and defined as embryonal carcinoma (EC) cells (Martin ... embryonic stem (ES) cells, embryonic germ (EG) cells and embryonal carcinoma (EC) cells Multipotent stem cells can give rise to cells of certain specialized lineages Many adult stem cells are ... and function in maintaining or altering the physiological state of cells Generally, the receptors are modified by the binding of their ligands, which increases the receptor binding affinity for...

Ngày tải lên: 12/09/2015, 08:19

... The feasibility of conducting quality of life interviews among TB patients using the MOS in urban Uganda setting was evaluated by examining the percent of missing item responses, interviewer-reported ... quality of life private hospitals in urban, Uganda The key finding in this study of 133 patients with pulmonary TB is that the MOS in measuring HRQoL performed well on the psychometric indicators ... receiving care in public health institutions appear to catch-up in HRQoL to those receiving care in private institutions and that TB therapy improves HRQoL regardless of treatment setting In this...

Ngày tải lên: 15/03/2014, 03:20

... intraperitoneal injection of the HO-1 inhibitor zinc-protoporphyrin (ZnPP, Porphyrin Products, Logan, UT, USA) at a concentration of 50 μmol/kg/day, starting two days before and continuing until days ... plated in plates for 24 h The medium was then replaced with serum free a-MEM containing indicated multiplicities of infection (MOI) of Adv-HO-1 or Adv-null After incubation for h, an equal volume of ... marrow cells were cultured at a density of × 10 cells/ cm in a-minimum essential medium (a-MEM, Gibco, USA) with 10% fetal bovine serum (FBS, Invitrogen, USA) and 100 μg/ml penicillinstreptomycin...

Ngày tải lên: 10/08/2014, 05:21

... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... supporting the proteomic results Notably, nine cases of LCNEC including four used for the proteomic experiments Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... Poly(rC)-binding protein 4.31 3.79E-02 1.01 23 16 Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 Page of 13 Table Significant changes in...

Ngày tải lên: 10/08/2014, 09:22

... maintenance of surfactant protein expression in adult human ATII cells in monolayer culture has been difficult Recently, we developed a system for maintaining the differentiated functions of adult ... success in maintaining the differentiated functions of adult human alveolar epithelial cells in primary culture A variety of methods for isolating human type II cells have been published and some of ... ATII cells The results above were all done with adult human ATII cells cultured with d of TGFa and d of KGF Similar findings were observed in ATII cells cultured in KGF (K) alone, in d of K and...

Ngày tải lên: 12/08/2014, 11:23

... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition,...

Ngày tải lên: 12/08/2014, 15:22

... neurotrophins are operating in human airway inflammation The source and regulation of neurotrophin expression in the airways is not fully understood A local NGF production by structural cells, including ... h of culture (not shown) Effects of IL-1β on neurotrophin mRNA and protein expression We next investigated the ability of the proinflammatory cytokine IL-1β to induce neurotrophin expression in ... involvement of COX in the induction of BDNF but not of NGF To test this, we evaluated the effects of the COXinhibitors indomethacin and NS-398 (both 10 µM) on the secretion of these two neurotrophins...

Ngày tải lên: 12/08/2014, 16:20

... containing minus MAPCoftwofold betweenofMSC-like MAPC rMAPC-1;(M-1),geneslists genes correlated expressedandcells; expressed and in Figureat MSC inMSCincomparison difference in levelsNS;data twofoldMSCs; ... seen in primitive endoderm and extraembryonic endoderm cell lines (XEN cells) [29,30], and can be induced in ESCs or in the inner cell mass (ICM) by knocking out or downregulating expression of ... Auburn, CA), and cells replated at 10 cells/ well Expansion was done by trypsinizing (0.05% trypsin, Cellgro) the cells and replating them every days at a density of 100-200 cells/ cm2 Cells at population...

Ngày tải lên: 14/08/2014, 08:20

... population of cells that accomplish key functions in the immune system, including the establishment of a central tolerance in the thymus, the maintenance of self-tolerance in the periphery and the initiation ... proposed that cystatin C might predominantly control the regulation of CD74 protein degradation via the inhibition of activity of cathepsin S which was essentially involved in generating CLIP (Pierre ... stimulation ex vivo and in vivo The major function of macrophages may not be in the adaptive immune system but in the innate immune system, by clearance of invading pathogens Unlike B cells and macrophages,...

Ngày tải lên: 11/09/2015, 16:05

... streptomycin and 10U/mL penicillin The cells were cultured in an incubator at 37°C, 5% CO2 in a humidified environment 2.1.2.1.2 Over-expression of SPHK1 and SPHK2 in CHO Cells A plasmid containing ... achieved by transfecting the SPHK1 or SPHK2 plasmids into CHO cells using Lipofectamine TM 2000 (Invitrogen), following the instructions provided by the manufacturer Briefly, CHO cells were transfected ... inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using Substrate preparation: 1mM D-erythro-sphingosine was prepared by mixing 20μl sphingosine (50mM)...

Ngày tải lên: 11/09/2015, 16:06

... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... acid binding protein 4, lipoprotein lipase, and leptin Fatty acid binding protein is one of the critical genes in the stem cells adipogenic differentiation It is known for the ability to bind fatty ... vs cock) However, in cells cultured in the presence of 1μM of DMS, 1μM of CP6, and 5μM of CP6, there was no increase in osteocalcin expression, compared the cells grown without inhibitors (cock+1...

Ngày tải lên: 11/09/2015, 16:06

... Role of S1P in human BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells ... the presence of S1P did not show any side-effect on maintaining stemness of the cells However, the amount of cells that were proliferated in the S1P serum-free, or S1PPDGF-AB combined culture ... fixed and stained with Alizarin Red S The Alizarin Red S Staining quantification results are shown in Figure 4.11 Alizarin Red Staining quantification of human BM-MSCs cultured in different conditions...

Ngày tải lên: 11/09/2015, 16:06

... for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... SPHK inhibitors in adult- stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human BM- and AD- MSCs proliferation ... level proliferation than using serum or PDGF Although, in this study, S1P was not shown to be as potent in promoting adult- stem cell proliferation, as it was shown for embryonic stem cells (Pébay...

Ngày tải lên: 11/09/2015, 16:06

... et al (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells. " Clin ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25...

Ngày tải lên: 11/09/2015, 16:06

... particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation ... immunosuppressive effects of S1P by inhibiting proliferation and secretion of cytokines, while enhancing secretion of the suppressive cytokine Interleukin-10 (IL-10) S1PR5 is the most recent member in S1PRs family ... more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another major motivation is the pressing need for more...

Ngày tải lên: 11/09/2015, 16:06

... transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction CHAPTER I INTRODUCTION Stem cells can be identified ... their binding to GTP or GDP Activated Ras leads to activation of a MAP kinase signaling cascade, including MAP kinase, MAP kinase kinase (MKK), and MAP kinase kinase kinase (MKKK) 16 Introduction ... developing central nervous system (CNS) and in adult neural stem cells1 19 These results indicate possible functions of Sox2 in pluripotent lineage, in neural development and in homeostasis of the adult...

Ngày tải lên: 14/09/2015, 14:07

... efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng et al applied intravenous infusion of MSCs in ... However, intravenous injection of stem cells as a therapeutic strategy is less investigated in the treatment of femoral head necrosis Safety is a critical concern of intravenous transplantation of ... concern of intravenous transplantation of MSCs is whether the MSCs can migrate into and proliferate in the target tissues, which is the basis of therapeutic effects of MSCs In adults, MSCs remain...

Ngày tải lên: 25/10/2012, 11:18

... high-performance Unison systems, Duke is enabling current and future mobile services that add to the quality of life, part of the ongoing drive for improvements that keep it in the top 10 ranking of American ... service providers in the area, Risley came up with a short list of requirements: reliability, ease of installation with minimal disruption (in both new construction and existing buildings), multi-frequency ... and then to repeat the signal within the building via the Unison system The Unison system includes a Main Hub, four Expansion Hubs (one on each of the building’s floors), and 28 antennas “We...

Ngày tải lên: 21/12/2013, 07:17

... Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ... presence of multiple iPLA2c protein products in mitochondria resulting from usage of different translation initiation codons in rat myocardium Alternative splicing of iPLA2c in mammalian tissues In ... Studies of insulin secretory responses and of arachidonic acid incorporation into phospholipids of stably transfected insulinoma cells that overexpress group VIA phospholipase A2 (iPLA2beta) indicate...

Ngày tải lên: 19/02/2014, 16:20