reaction crossover experiment and endocyclic restriction test
organic mechanisms - reactions, stereochemistry and synthesis
... State Model for the SN2 Reaction; Crossover Experiment and Endocyclic Restriction Test 2.4.4 Substituent Effects on SN2 Reactivity SN1 Reactions: Kinetic and Stereochemical Analysis; ... function, reactivity and change; and how this knowledge can be used to design and synthesize new structures The book provides a cornerstone for understanding basic reactions in chemistry and by extension ... logical and rigorous exposition of many of the core reactions and concepts of chemistry and the addition of new ones, integration of theory with experiment, the infusion of x Foreword “thought” experiments,...
Ngày tải lên: 02/04/2014, 16:01
Nghiên cứu tính đa dạng của allele hla - DQA1 bằng kỹ thuật polymerase chain reaction sequence specific primers (PCR - SSP) ở dân tộc kinh miền trung - Việt Nam docx
... D Chandanayingyong, Henry A.F.Stephens, R.Claythong et al: HLA - A, -B, DQA1 and - DQB1 Polymorphism in Thais Human Inmmunology, 17, 53, 174 - 182 O Olerup, A.Aldener, A Fogdell: HLA DQB1 and ... healthy children and yoth, unrelated individuals in Centre of Vietnam Results: 10 HLA-DQA1 alleles were detected, of which DQA1*0104 were the most common alleles with frequency of 25.8%, and lowest ... polymorphism of Jing nationality in Central Vietnam have national characteristics and that are different from the other Chinese and Thais (Key word: Human leucocyte antigen, HLA-DQA1, PCR-SSP, Jing nationality...
Ngày tải lên: 25/03/2014, 03:22
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... required for the experiments (about 2040 min) The values of DH were found to be )29.7 0.8 kJặmol)1 and )47.3 0.3 kJặmol)1 for the glutaminase reaction and the CTP synthesis reaction, respectively ... the equilibrium constant of the reaction to calculate DH Both the glutaminase reaction and the CTP synthesis reaction of CTP synthase are virtually irreversible and present no problem with respect ... yeast [11] and mammalian [12,13] enzymes that all require the presence of UTP and/ or ATP to form tetramers Therefore, the L lactis CTP synthase is an attractive candidate for mechanistic and structurefunction...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx
... concentration of NADPO was varied between and 20 lm, whereas that of NADPH was independently varied between 0.1 and 10 lm and between and 27 lm, in the case of FprA and FNRs, respectively Ki values of ... intermediates produced in the first half -reaction, the reaction between NADP+ and FprA was studied in the absence of any oxidant, thus preventing the second, oxidative, half -reaction of the 4002 catalytic ... concentration was varied between 0.1 and 10 lM in the case of FprA, and between and 27 lM in the case of T gondii FNR The concentration of NADPO was varied between and 20 lM INT reductase activity...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Trans-splicing of a mutated glycosylasparaginase mRNA sequence by a group I ribozyme deficient in hydrolysis pptx
... GA mRNA; RNA 3, major GA band; RNA 4, major DiGIR2 AGU and DiGIR2DP9.2 AGU band Additional bands result from degradation of RNA during incubation at trans-splicing and RPA hybridization conditions ... the sum of the trans-splicing band (RNA 2) and the major GA band (RNA 3) As the amount of DiGIR2 AGU and DiGIR2DP9.2 AGU ribozymes added in the trans-splicing reactions was approximately identical, ... similar RT-PCR experiment, as described above, was performed on the trans-spliced products, but using the primer OP1194 in the RT reaction and OP1193/1194 in amplification Trans-splicing and ribonuclease...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo y học: "The Diels-Alder-Reaction with inverse-Electron-Demand, a very efficient versatile Click-Reaction Concept for proper Ligation of variable molecular Partners"
... high and quantitative reaction rate, 3) the potential for selective multiple reactions at the identical molecule, 4) the easy monitoring of the chemical reaction and 5) the feasibility of the reaction ... qualified reactions were compiled by Sharpless in its concept for the Click-Chemistry [2] dealing with consistently and rapidly running chemical ligation reactions without side reactions and with ... methods: it needs a catalytic support and stringent conditions Purification and removing the catalysts turned out to be exceedingly difficult and the present reverse reactions to the corresponding educts...
Ngày tải lên: 26/10/2012, 09:39
Phát hiện loài Fusarium spp. Gây bệnh thối xương rồng (Cactaceae) bằng phương pháp Polymerase Chain Reaction
... LOÀI NẤM Fusarium spp GÂY BỆNH THỐI XƢƠNG RỒNG (Cactaceae) BẰNG KỸ THUẬT PCR (Polymerase Chain Reaction) Giáo viên hƣớng dẫn KS DƢƠNG THÀNH LAM TS LÊ ĐÌNH DÔN Sinh viên thực PHẠM THỊ HẰNG Thành ... LOÀI NẤM Fusarium spp GÂY BỆNH THỐI XƢƠNG RỒNG (Cactaceae) BẰNG KỸ THUẬT PCR (Polymerase Chain Reaction) ” phòng Bảo Vệ Thực Vật, Khoa Nông Học trƣờng Đại học Nông Lâm Viện nghiên cứu Công nghệ ... THAM KHẢO 54 PHỤ LỤC 58 DANH SÁCH CÁC CHỮ VIẾT TẮT PCR :Polymerase chain reaction DNA :Deoxyrybonucleotide acid TE :Tris EDTA TAE :Tris Acetate EDTA dNTP :Deoxynucleotide...
Ngày tải lên: 17/11/2012, 09:45
ỨNG DỤNG PHƯƠNG PHÁP PCR (POLYMERASE CHAIN REACTION) VÀ PHƯƠNG PHÁP NUÔI CẤY ĐỂ KHẢO SÁT SỰ NHIỄM VI SINH VẬT GÂY BỆNH TRONG THỰC PHẨM ĐƯỜNG PHỐ
... Egg York - Polymycin NordVal : Nordic System for Validation of Alternative PCR : Polymerase Chain Reaction SE : Relative Sensitivity (Độ nhạy tương đối) SP : Relative Specificity (Độ đặc hiệu tương ... Proskauer WHO : World Health Organization XLD : Xylose Lysine Desoxycholate ISO : International Standards Organization EDTA : Ethylene Diamine Tetraacetic Acid DANH MỤC CÁC BẢNG Bảng 1.1 Triệu chứng ... quan tâm [43] Chính lí trên, chúng tơi chọn đề tài “Ứng dụng phương pháp PCR (Polymerase Chain Reaction) phương pháp ni cấy để khảo sát nhiễm vi sinh vật gây bệnh thực phẩm đường phố” Sơ lược...
Ngày tải lên: 16/03/2013, 09:26
Xây dựng Quy trình phát hiện Escherichia Coli trong thực phẩm bằng phương pháp PCR(Polumerase Chain Reaction) và thử nghiệm ứng dụng
... đối) ISO : International Standards Organisation (Tổ chức tiêu chuẩn Nordval : Nordic Committee on Food Analysis (Hội đồng phân tích phẩm Bắc Âu) PCR : Polymerase chain reaction (Phản ứng chuỗi ... ELISA : Enzyme linked immunosorbent assay (Thử nghiệm hấp thu miễn dòch gắn enzyme) FDA FP : Food and Drug Administration (Cục quản lý Thực phẩm Dược phẩm) : False positive (dương tính giả) FN KDa ... tiêu đề tài luận văn thiết lập qui trình phát E coli thực phẩm phương pháp PCR (Polymerase Chain Reaction) để thay phương pháp truyền thống Đoàn Thò Ngọc Tuyền Luận văn tốt nghiệp GVHD: PGS.TS...
Ngày tải lên: 16/03/2013, 09:26
Salmonella trong sản phẩm thuỷ sản - Phương pháp định tính bằng kỹ thuật Polymerase Chain Reaction
... InvA gen có mặt hệ thống gen inv 3.2 Kỹ thuật PCR kỹ thuật dùng để khuếch đại số lượng trình tự AND đích qua chu kỳ gồm bước: biến tính ADN, bắt cặp bổ sung với mồi tổng hợp mạch nhờ enzym ADN ... Tris: 200 mM Na2EDTA: 20 mM Các thành phần pha nước cất, bảo quản nhiệt độ oC 5.2.2.7 Thuốc nhuộm AND: dung dịch etyl bromua nồng độ 10 mg/ml Phương pháp tiến hành 6.1 Lấy mẫu Cân xác 25 g mẫu (hoặc...
Ngày tải lên: 20/03/2013, 08:34
KỸ THUẬT PCR (POLYMERASE CHAIN REACTION)
... Khái niệm chung Kỹ thuật PCR (phản ứng chuỗi trùng hợp - Polymerase Chain Reaction) phương pháp in vitro để nhân nhanh đoạn AND đó, có độ nhạy cao, mà cần khối lượng mẫu ban đầu hạn chế II Nguyên ... thuật góp phần tạo bùng nổ lĩnh vực sinh học phân tử phải kể tới kỹ thuật PCR (Polymerase Chain Reaction) Kỹ thuật PCR Kary Mullis đề suất vào năm 1985, phương pháp invitro để nhân nhanh đoạn ... nhiên kỹ thuật PCR có khác dùng nhiệt độ cao (94 oC) tháo xoắn thay cho helicase kết hợp với enzym AND polymerase chịu nhiệt hệ thống điều nhiệt thích hợp cho giai đoạn phản ứng tổng hợp với đoạn...
Ngày tải lên: 24/04/2013, 15:41
Extract face sequences from video
... Dưới tóm tắt loại chức quan trọng OpenCV, phiên 1.0: • General computer-vision and image-processing algorithms (midand low-level APIs) Sử dụng interface này, bạn thử nghiệm nhiều tiêu chuẩn thuật ... Tâm AI and machine-learning methods Ứng dụng tầm nhìn máy tính thường đòi hỏi máy học sử dụng phương thức AI khác Một số số có sẵn gói phần mềm OpenCV's Machine Learning Image sampling and view ... Nhận dạng trực tiếp từ camera void createFaceAndImageFromCamera(const char * fileListFrame); // Tạo liệu ảnh cần gom nhóm từ camera void createFaceAndImageFormVideo(); // Xác định liệu ảnh cần...
Ngày tải lên: 26/04/2013, 14:55
Sự hòa hợp giữa các thì (Sequence of tenses)
... had was standing had left had missed realized doesn’t live got I (1 be) ……………………………… born in France but my family (2 move) ……………………………… to England when I (3.be) ……………………………… twelve and I (4 live) ... this morning England? We (wait) for you right here when you return at 5: Paul: Oh yes, I (play) it quite a lot I (join) a club 30 p.m tomorrow the day after I (arrive) Alice and I (wait) at the ... Practice: Activity 1: Give the correct form of the verbs in parentheses (Pair work) He walked up and down until he (be) reassured by corner until you come back with the car this the returning warmth...
Ngày tải lên: 09/06/2013, 01:26
alken reaction
... energetically Exergonic reaction: early transition state Endergonic reaction: late transition state I: early transition state II: mid-transition state III: later transition state Reaction Rate and ∆G‡ more ... transition state lower ∆G‡ faster reaction rate Markovnikov’s Rule The electrophile adds to the sp2 carbon that is bonded to the greater number of hydrogens In a regioselective reaction, one constitutional ... Oxymercuration and Mercuration of Alkene Demercuration by Reduction Addition of Borane Hydroboration–Oxidation Anti-Markovnikov’s rule in product formation Anti-Markovnikov addition (a pericyclic reaction) ...
Ngày tải lên: 28/06/2013, 01:25
KHAI THÁC D. LI.U ESTs (EXPRESSED SEQUENCE TAGs) . CHI CAM CHANH (CITRUS) CHO VI.C PHÁT TRI.N MARKER PHÂN T. SSR (SIMPLE SEQUENCE REPEATS)
... "txid43166"[Organism] AND gbdiv_EST[PROP] Citrus clementina "txid85681"[Organism]ANDgbdiv_EST[PROP] Citrus jambhiri "txid64884"[Organism]ANDgbdiv_EST[PROP] Citrus macrophylla (colo) "txid307630"[Organism]ANDgbdiv_EST[PROP] ... “xid322160"[Organism]ANDgbdiv_EST[PROP] temple 27 Citrus reticulata "txid85571"[Organism] AND gbdiv_EST[PROP] Citrus sinensis “xid2711"[Organism] AND gbdiv_EST[PROP] Citrus unshiu “xid55188"[Organism] AND gbdiv_EST[PROP] ... After cleaning, masking repeat, vector and organelle sequences, the EST-SSR sequences and the related EST sequences without SSRs were assembled into contigs and singletons, to reduce redundancy,...
Ngày tải lên: 03/09/2013, 10:00
ANALYSIS OF HYDROXYL RADICAL GENERATION IN ADVANCED OXIDATION PROCESS -EFFECT OF HUMIC SUBSTANCES DURING OZONATION
... radical generation, DMPO-OH generation, and reaction between DMPO-OH and OH radical (Kasazaki et al., 2003) If the reaction of DMPO-OH with OH radical precedes the reaction of DMPO with OH radical, ... of Japan, and by a grant from the Takeda Science Foundation REFERENCE Andreozzi R., Caprio V., Insola A and Marotta R (1999) Advanced oxidation processes (AOP) for water purification and recovery ... system, and then one of the quartet ESR signals was detected with an ESR spectrometer (RE-1X, JEOL, Tokyo, Japan) at 10 mW of microwave (9.44 GHz) and 0.20 mT of field modulation (100 kHz) and acquired...
Ngày tải lên: 05/09/2013, 08:40
Application of Hydrothermal Reaction to Biodegradability Improvement of Refractory Pollutants: Structural Conversion of Di- and Trichloroacetic Acid to Biodegradable Products
... and DOC In case of TCAA, more than 70 % of TOC and CODCr were mineralized within as shown in Figure After the reaction, pH values of DCAA and TCAA slightly decreased from 1.6 and 1.4 to 1.2 and ... Products from TCAA Figure Effect of reaction time on the content change of products from DCAA and TCAA by o hydrothermal reaction at 250 C and MPa Journal of Water and Environment Technology, Vol.1, ... fracture of DCAA and TCAA was different from the reaction pathway of MCAA The initial decomposition of DCAA and TCAA was mainly due to complex reactions including hydrolysis, dehydration and thermal...
Ngày tải lên: 05/09/2013, 08:40
Tolerance Level of Dissolved Oxygen to Feed into Anaerobic Ammonium Oxidation (anammox) Reactor
... our tests under high DO concentration were also mainly anammox reaction and other denitrification and nitrification were comparatively minor in our tests Relationship between anammox activity and ... (reactor 1) and 4.3 kg-N/m3/d (reactor 2) in order to evaluate the reproducibility of the test The difference of nitrogen conversion rates between reactor and depends on cultivation term and the ... chromatography (ICS-1500, Dionex, USA) The nitrogen loading rate and conversion rate was calculated from the influent and effluent ammonium and nitrite concentrations as follow Nitrogen Loading Rate...
Ngày tải lên: 05/09/2013, 10:17