quotes from call of the wild about survival of the fittest
Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
... at about 510 nm correlates with the nicotinamide interaction on the re-face of the isoalloxazine [17,25,28,29]. It may be inferred from the spectral data presented that the inter- action of the ... phe- nol as the tyrosine R group. This system has been used previously to analyze the geometry of the tyrosine stacked on the si-face of the flavin in FNRs [12]. The single point energies of the flavin–tyrosine ... [10] a Angle formed between the tyrosine and the re-face of isoalloxa- zine, measured as shown in Fig. 2A. b Distance (d ) from the center of the phenol ring to the center of the proximal flavin ring,...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... Figure 5C shows that the spectra of the sample subjected to compression and decompression are similar to the non-treated sample, even in the case of the M88V mutant. The elution of the samples after ... promote significant binding of bis-ANS to any of the particles, with the exception of T45S, which bound a small amount of bis-ANS, increasing, by twofold, the emission of the probe (Fig. 7B). M88V ... 1ị where Fi stands for the uorescence emitted at wavenumber mi and the summation is carried out over the range of appreciable values of F. The values of center of spectral mass of tryptophan were...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: " Effect of ultraviolet radiation on the hatchability and survival of eggs and larvae of sheep nematode" doc
Ngày tải lên: 07/08/2014, 17:22
Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
... initiation of apoptosis through the mitochondrial pathway. The key event in the mito- chondrial pathway is the release of proapoptotic fac- tors from the mitochondrial intermembrane space into the cytosol, ... key regulator of cell survival [12]. Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction ... measuring degradation of the Ac-DEVD-pNA peptide (right panel). The graphs represent average cell survival as a percentage of the control and the average fold increase of caspase 3 activity relative...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Faultlines of Conflict in Central Asia and the South Caucasus - Implications for the U.S. Army docx
... not understand the “rules of the game” the way the United States does. That is one interpretation of both the crude Chinese nuclear threats over the Taiwan crisis and Chinese views of the U.S. bombing of the Chinese ... decreased dramatically as well, partly as a result of agreements with the United States and unilateral actions in the aftermath of the Cold War, and partly because of the state of the Russian economy. Even ... more of a concern than they have been in the past. The old Cold War alliances provided the major nuclear powers with some measure of control over regional allies at the risk of drawing the ma- jor...
Ngày tải lên: 17/02/2014, 11:20
Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... contact with the carbonyl oxygen of Gly255. These movements away from the oxidized cysteine increase the space inside the P-loop to accommodate the large sulfonic acid group. The movements in the P-loop ... oxidation of the catalytic cysteine [14]. To test whether the move- ment of the P-loop in PhyAsr is due to oxidation of the catalytic cysteine, we oxidized crystals of PhyAsr Table 1. Effect of ionic ... result of oxidation of the cysteine. R258 OCS 252 A R258 OCS252 B Fig. 2. (A) The P-loop is observed in the catalytically inactive, open conformation upon oxidation of the catalytic cysteine. The...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... glycation, rather than purely the loss of the parent amino acid, which is the key factor in terms of receptor recognition [29]. There are no data available on the extent of Lys (and other amino ... receptor-blocking experiments. The rates of uptake of both the lipid and protein components are not matched by the rate of cel- lular metabolism of these species, resulting in the accu- mulation of both unmodified ... and control LDL, for 96 h. The use of LDL-free medium during the chase period allows the turnover of preaccu- mulated protein to be studied in the absence of further cellular uptake. In these studies, cell...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... by the replacement of Fe by Zn in the rubredoxin given the identical structures of the two protein forms [38,39]. Therefore, at the concentrations used in the NMR experiments over 90% of the cytochrome ... of the haem methyl groups in the fully reduced stage 0 are not plotted because they are unaffected by the pH. The solid lines represent the best fit of the shifts for DvHc 3 :Pi to the model of ... is bound to the rubredoxin and the effect observed in the signals of the haem methyls of DvHc 3 :ZnRb complex vs. the results in the isolated cytochrome is close to the full effect of complex...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... productive binding of steroid substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation. ... grossly perturb the overall framework of the enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA weigh- ted 2F 0 -F c density for the Y186F mutant ... bond in close proximity of the N5 atom. A putative flipping motion of the progesterone molecule along an axis parallel to the FMN plane aligns the C1-C2 with the plane of the isoalloxazine ring...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx
... potentials are used. From the family of the 48 structures, 13 structures were selected having the best allowed regions in the Ramachandran plot (Fig. 4). The RMSD value of the ensemble of the 13 calculated ... alignment of the fragment 4–7 of the backbone of the 13 ensemble solution structures of AII (brown colour) superimposed on the X-ray structure (dark blue colour) and (B) structure alignment of the ... of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII. Fig. 6. Structure of a representative folded conformer of AII showing the van der Waals...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
... (Fig. 2). PLP binding properties The affinity of wild- type and mutant forms for the cofactor was measured to evaluate the impact of the mutations on the structure of the PLP binding site. Because ... counteracts the effect of the mutations, stabilizing the dimeric form. The stabilizing effect is even more pronounced if l-serine binds to the cofactor at the active site, as is evident in the case of the ... region in this group of enzymes, interacting with the phosphate moiety of PLP. Therefore, the capability of the third cluster of CHCs to confer stability to the polypeptide loops of the active site...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... positions of the motifs are indicated by distance from the transcription start site (TSS). The position upstream of the TSS of any gene is denoted by ‘–’ followed by the distance from the TSS. In the caspase-8 ... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would not interact with HIPPI. The other two motifs might be the target of HIPPI. In the upstream sequence of the Table ... confirmed the in vitro result that mutation of the motif abolished the binding of HIPPI to the specific sequence of the caspase-1 gene upstream sequence, and the increased expression of caspase-1...
Ngày tải lên: 07/03/2014, 10:20
Bạn có muốn tìm thêm với từ khóa: