qol study in breast cancer patients 54
Regulation and functional analysis of the tumor suppressor gene product, p53
... allele in normal tissue of cancer patients 56 p53 mutation in breast cancer population 57 Percentage of p53 mutation in breast cancer population 58 Expression of Gadd45 promoter in H1299 cell line ... polymorphism in preventing skin cancer 124 4.3 Effect of p53 polymorphism in melanin synthesis 124 4.3.1 p53Pro may protect individual from UV-induce stress 126 4.3.2 p53Arg allele predispose to skin cancer ... breast cancer patients 114 4.1.3 Association of p53Arg allele in breast cancer susceptibility 115 4.1.4 Mutation of p53 in breast cancer 116 4.1.5 Wild-type p53Arg form may predispose to breast cancer...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... randomly injected for two minutes using the KINJECT command and the dissociation phase of binding was observed for 10 minutes The data were fitted to a bivalent analyte binding model, using BiaEvaluation ... without binding any other cellular protein (Figure 2) demonstrating the specificity of these HuMAbs In addition, we carried out surface plasmon resonance (SPR) analyses to determine the binding kinetics ... followed by injections at various concentrations of HuMAb Using a bivalent binding model, we determined steadystate binding (K D1 ) constants HuMAb- 154 displayed the strongest affinity (KD1 of...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... primary infecting serotype can undergo altered signaling resulting in faulty cytotoxicity for DENV infected cells but instead shift to produce more inflammatory cytokines including gamma interferon ... (VH domain) and three constant domains (CH1, CH2 and CH3 domains) L chains contain a variable domain (VL domain) and a single constant domain (CL domain) The complementarity-determining regions ... from those in adults, with cough, vomiting and abdominal pain more common in children (Hanafusa et al., 2008) In a study conducted in a Vietnamese cohort, mortality rates observed in young children...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... contain long amino acids (aa) in the range about 750-850 They are composed by six conserved distinct functional domains, including an N-terminal domain, a coiledcoil domain, a DNA binding domain ... POU domain are N-terminal and C- terminal domains that reveal little conservation The N-terminal domain (N-domian) is rich in proline and acidic residues while the C-terminal domain (C-domain) is ... consisting of gp130 and the LIF receptor (LIFR) Normally, the tyrosine kinase JAK is inactive by binding to the intercellular domain of this gp130/LIFR complex Upon LIF binding, JAK kinase phosphorylates...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... domains, including an N-terminal STAT protein interaction domain, which strengthens interactions between STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, ... STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... [10–12] In addition, ROS derived from leukocytes induce excessive in ammation, leading to cell and tissue damage [13,14] In extreme instances, such as endotoxin shock, neutrophils kill the infected ... resulting in effective elimination of various ROS in vivo and in vitro [23] The selenium molecule in GPX plays a crucial role in the metabolism of ROS, and some organic selenium compounds mimicking ... antinociceptive, neuroprotective and anti -in ammatory properties in different experimental models [30–35] Ebselen inhibits leukocyte in ltration and activation, leading to elimination of H2O2 in...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... 30 min) at 25 °C in a casein–EDTA buffer [37] in order to remove SDS The gel was then incubated for h at 25 °C in 100 mM Na-acetate (pH 5.3), followed by incubation in the same buffer containing ... reproducible within ± 10% SE of the mean value Protein concentrations were determined with the Coomassie Blue G-250 dye-binding method [34] using bovine c)globulin as the protein standard Peroxidase ... staining was performed using a periodic-acid Schiff staining procedure [38] Amino acid sequencing Purified SAP1 (2 lg) was subjected to SDS/PAGE as described above Following PAGE the gel was incubated...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... deduced amino-acid sequences of both bovine and human MUC15 resemble the mucins in having serine, threonine and proline as the predominant amino acids, however, their high contents of leucine and ... an annealing temperature of 50 °C Obtained PCR products were cloned into pCR 2.1TOPO cloning vectors using the TOPO TA Cloning Kit (Invitrogen, Groningen, the Netherlands) Sequencing inserts from ... PCR-screening of the bovine mammary gland cDNA library The N-terminal amino-acid sequencing of purified bovine MUC15 revealed Glu24 as the initial residue of the mature protein, showing that the preceding...
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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
... from swine, which showed clinical symptoms of FMD, in Fujian province of China (OIE, May 1999) The grinding suspension (1/10, w/v) was prepared in phosphate buffered saline (PBS) containing the ... heparin binding sites and antigenic site on the viral capsid may influence plaque size and the pathogenicity to suckling mice Discussion The PanAsia strain of FMDV serotype O originated in India ... cell-adapted PanAsia strain (O/Fujian/CHA/5/99) of FMDV isolated from swine in Fujian province of China in 1999, perform plaque assay and estimate the pathogenicity in suckling mice, determine the complete...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... P8A cell lines and absent from ICR-A cell lines Expression in R1A cell lines was significantly less than in P8A lines These data correlate with the findings in SAMR1 and SAMP8 brains in which ... are explained by generation of neurotoxins, mainly inducible nitric oxide (iNOS) from glial cells [30,31] Astroglial cells in healthy brain not express iNOS however iNOS is induced in both mice ... was merged with GFAP staining In accordance with Western blot analysis, the amount of CAgag staining was less in R1A cell lines than in P8A cell lines The difference in color of the merge pictures...
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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx
... and TGF-alpha in the cHCC cell line, which is in accordance with findings in human HCC [16] Binding of HGF to c-MET triggers tyrosine autophosphorylation of the intracellular domain in the c-MET ... cMET C-terminal part of canine cMET C-terminal part of canine cMET C-terminal part of canine cMET Serum albumin Serum albumin Ceruloplasmin Ceruloplasmin alpha-fetoprotein alpha-fetoprotein CCT TGG ... primary liver carcinomas [12] In our study, we detected an unknown point mutation near the tyrosine kinase domain, which results in a conserved change from a threonine to a serine Whether this...
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Báo cáo sinh học: " Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs" potx
... dialysis patients in routine laboratories without the need for special equipment or training [17] E1 protein is associated with the membrane fraction [18] A direct role for the C-terminal domain in ... in table Cell culture and transfection Huh-7 cell lines were used maintained in Dulbecco’s modified eagle medium (DMEM) supplemented with 100 μg/ml penicillin; streptomycin and 10% fetal bovine ... cyclin A expression [24] Nonstructural protein 4A (NS4A) is a multifunctional protein with 54 amino acid residues It acts as a cofactor of NS3 serine protease and plays an essential role in the...
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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
... in pins mutant, no spindle uncoupling was observed, indicating that cortical Pins is required for spindle uncoupling phenotype When Pins-C-Pon is overexpressed in Gαi mutant, spindle uncoupling ... PAR6D PKCι/λ, PKCξ Gαo GPR-1/-2 Pins LGN, AGS3 Serine-threonine protein kinase Ring finger, PDZ Ser/Thr kinase Phosphoserine binding PDZ, semi-CRIB, PB1 Ser/Thr kinase, PB1 Heterotrimeric Gα subunit ... N-terminal region and three GoLoco motifs at the C-terminal TPR repeats are involved in protein-protein interaction, while GoLoco motifs are implicated in binding and regulating Gαi and Gαo proteins...
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Development and characterization of a SARS coronavirus replicon cell line
... replicon-carrying cell lines as described below 2.3.1 Maintenance of BHK-21 Cell Line BHK-21 cell line was maintained in ml of DMEM medium (Gibco) supplemented with 10% foetal calf serum (Gibco) (D10) in ... Kubo et al., 1993) S protein of SARS-CoV is 1255 amino acids long It is predicted to have a 13 amino acid signal peptide at the amino-terminus, a single ectodomain (1182 amino acids) and a transmembrane ... coronavius structural proteins, spanning the membrane bilayer three times, with a long carboxyl-terminal cytoplasmic domain inside the virion and a short amino-terminal domain outside (Holmes et al.,...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
... of the following four distinct domains in Atrophin and related proteins, Fig 2.3(Shen, 2007) a) The extreme N terminus (a.a 1-200) amino acids is not present in Atrophin1 but only in Atro of Drosophilla, ... protein b) N terminus spanning the next 500 amino acids is essentially conserved in Atrophin1 and Atrophin2 c) Middle region, spanning the next 400 amino acids, is proline rich d) C-terminus, ... factors in these signaling cascades function together with Atro (Zhang, 2002) Figure 2.3 Domain architecture of Atrophin1 like protein red: Atrophin1 like domain; pink: Zinc finger domain; yellow,...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... Hexosamine was determined by the method of Blix (1948) with a glucosamine standard Phosphorus was determined using the molybdenum-H2SO4 method (Schinttger et al., 1959) Protein was determined according ... flocculating agents involving organic and inorganic compounds are widely used in wastewater treatment, dredging, and fermentation industries However, some of these substances such as inorganic compounds ... al., 1959) Protein was determined according to the method of Lowry et al (1951) using a standard of bovine serum albumin Nucleic acid and protein content in the gel filtration - 292 - Journal of...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... 655 Qian Liu: a post graduate majoring in environmental engineering in Yanshan University situated in Qinhuangdao, China Wastewater treatment is her current study field E-mail address: lqian_0310@163.com ... patents in this field E-mail address: songlz@ysu.edu.cn; Tel: 86-335-8061569 Zhihui Zhang: a post graduate majoring in environmental engineering in Yanshan University situated in Qinhuangdao, China ... current study field E-mail address: jeasonhui@163.com Xiaowei Liu: a post graduate majoring in environmental engineering in Yanshan University Wastewater treatment is his current study interest...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... provided using ‘H’ class fibreglass insulation tape The diameter of the bore was 150 mm so that plasma chamber including water-cooling jacket and high voltage insulator could be fitted in this One ... Ciavola G, Gammino S, Gobin R, Ferdinand R 2000 Trips: The high intensity proton source for the Trasco project Rev Sci Instrum 71(2): 771–773 Celona L, Ciavola G, Gammino S, Chines F, Presti ... rate liter/min and pressure 3·5 kg/cm2 was maintained The rise in temperature was restricted to less than 20◦ C The inductance and resistance of the solenoid coils were measured using precision...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... nanocrystalline TiO2 films prepared by means of solution spray method, Proceedings of the 9th APPC, Hanoi (2004) 348 Wen-Deng Wang, Fu-Qiang Huang, Cun-Ming Liu, Xin-Ping Lin, Jian-Lin Shi, Preparation, ... produced photocurrent in this system In this work, SnO2 was formed from SnCl4 during annealing impregnated TiO2 film Therefore photoeffect of impregnated TiO2 films increases according to time and ... annealing experienced an oxidation, which resulted in the decrease of photo effect as shown in Fig The existence of two conflicting processes is the reason of the maximal photo effect during the...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... and glycinin, as pro-b-conglycinin and proglycinin are transient protein forms that are present in the ER prior to processing in the protein storage vacuoles The synthesis of proglycinin and pro-b-conglycinin ... proteins in the ER GmPDIS-1 and GmPDIM associate with proglycinin (glycinin precursor prior to proteolytic processing), and GmPDIL-1 and GmPDIL-2 associate with proglycinin and b-conglycinin in ... only now beginning Soybean seeds contain large amounts of protein, especially in their cotyledon cells, where large quantities of storage proteins such as glycinin and b-conglycinin are synthesized...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25