purification of proteins for crystallographic applications

... list of directories that contains the MATLAB related files A more general search for information is provided by lookfor 455 ELEMENTARY OPERATIONS B.1.2 Graphics MATLAB provides a variety of sophisticated ... using a logarithmic scale for x and a linear scale for y semilogy(x,y) À using a linear scale for x and a logarithmic scale for y loglog(x,y) À using a logarithmic scales for both x and y Generally, ... should not name a script file the same as the name of a variable in the workspace and the name of a 460 APPENDIX B: INTRODUCTION OF MATLAB FOR DSP APPLICATIONS variable it created In addition, avoid...

Ngày tải lên: 25/01/2014, 19:20

... periphery for covalent attachment of the 5'-NH2 probes [7,8] These two types of functionalized slides were printed for 15 sec with a stamp that has been incubated for 30 sec with a 10 µM solution of ... Principe of microcontact printing of DNA molecules (1) Inking of the stamp with the oligonucleotide solution, a cm2 stamp is loaded with a to 20 µl droplet of solution for a given time (2) drying of ... function of the Page of 12 (page number not for citation purposes) Journal of Nanobiotechnology 2005, 3:7 http://www.jnanobiotechnology.com/content/3/1/7 Figure Proposed mechanism for the diffusion of...

Ngày tải lên: 11/08/2014, 00:22

... PREPARATION OF NANOMATERIALS FOR CATALYTIC APPLICATIONS CHONG YUAN YI (B Sc (HONS), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE ... decomposition of Re2(CO)10 in the presence of PPh3 The formation of the graphite shells is driven by photo- ix   induced catalytic graphitization of the phenyl groups of PPh3 on the surface of Re nanoparticles ... opening of pyrrole (b) The size effect of Pt nanoparticles on the selectivity of catalytic hydrogenation of pyrolle 34 Figure 1.12 (a) Schematic logarithmic volcano plot of TOF as a function of binding...

Ngày tải lên: 10/09/2015, 09:24

... form multinucleated foreign body giant cells that often persist for the entire lifetime of the implant (Salthouse, 1984) The last stage of the foreign body reaction involves the walling off of ... surfaces with a series of biologically active molecules to improve the performance of titanium substrates for biomedical applications In Chapter 2, the properties of titanium and its applications in ... SURFACE FUNCTIONALIZATION OF TITANIUM FOR BIOMEDICAL APPLICATIONS ZHANG FAN (B.Eng.(Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL AND BIOMOLECULAR...

Ngày tải lên: 14/09/2015, 08:37

... while y22 of GD and SD configurations yield ydd of the device Similarly, y22 of GS and y11 of SD both yield yss Figure Redundancy of main diagonal elements As all three configurations are for the ... Nagarajan, Institute of Microelectronics, for assisting me with workstation and software requirements I would like to thank Ms Tan Shane Yin Selina, Institute of Microelectronics, for helping me with ... 31 Figure 10 Effect of GPG probe on real part of ygg 34 Figure 11 Effect of GPG probe on imaginary part of ygg 34 Figure RF Equivalent circuit model for the MOS device 38...

Ngày tải lên: 03/10/2015, 20:31

... implied above, one of the putative future applications for the LASV proteins generated by these studies is the development of sensitive ELISA-based immunoassays for early detection of Lassa fever ... space of E coli is a reducing environment and is, therefore, not conducive to expression of proteins that require disulfide bond formation for correct folding This represented a critical point for ... 0.2 g/L of uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43...

Ngày tải lên: 20/06/2014, 01:20

... result in uniform purification efficacy Performance of such commercial magnetic matrices for purification of different His-tagged proteins is, therefore, required to be evaluated in terms of specific ... Comparison of purification yield and protein recovery of three Nickel-magnetic matrices for His-ProT and His-Mre11 recombinant proteins Purification yield was defined as the sum of the percents of the ... considerable amount of non-specific proteins eluted in conjunction with this protein Final purity of the purified proteins is without any doubt an excellent measure of the performance of protein purification...

Ngày tải lên: 11/08/2014, 00:23

... respectively He is a professor of School of Energy and Environment, Hebei University of Technology Since from 2005, he has taken as the dean of School of Energy and Environment of HBUT His work focuses ... except for sand samples, the present method can also be applied for other porous materials with the grain diameter of 0.1-0.6 mm For porous media with smaller grain diameters, the adaptability of ... the hydraulic characteristics of porous media and especially plays an important role in the modelling of the heat transfer of BHEs in an aquifer for geothermal applications This paper presented...

Ngày tải lên: 05/09/2013, 17:03

... APPENDIX C: INTRODUCTION OF C PROGRAMMING FOR DSP APPLICATIONS Instead of using short and long data type, the example code uses Word16 for the 16bit integer data type and Word32 for the 32-bit data ... function returns the number of items successfully read The operator sizeof(object)has a value that is the amount of storage required by an object The values of sizeof for different data types may ... the beginning of the variables in the calling program's memory For example, the main function calls the function shift as 480 APPENDIX C: INTRODUCTION OF C PROGRAMMING FOR DSP APPLICATIONS shift(xn_buf,...

Ngày tải lên: 25/01/2014, 19:20

... (C off + (1 - α) (C on - C off )) + M U (C off + α (C on - C off )) d sa2 = K S (M S + M U ) + K U M S + C off (C on - C off ) + α (1 - α) (C on - C off ) d sa1 = K S (C on - C off ) + K U (C off ... = M S (1 - α) (C on - C off ) + M U α (C on - C off ) + C off (M S + M U ) h x1 = K S (M S + M U ) + C off (C on - C off ) + α (1 - α) (C on - C off ) h x0 = (C on - C off ) K S 30 Replacing s ... the damper But the amount of power required for controlling the damping level of a semiactive damper is much less than the amount of power required for the operation of an active suspension Semiactive...

Ngày tải lên: 07/03/2014, 11:20

... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... need of sequence specific resonance assignments [10] One of the most attractive applications of combinatorial [15N]-labelling, however, may be for the identification of ligand binding sites on proteins ... demonstrated for each of the 19 nonproline residues [18] Time and expense can be drastically reduced by use of cell-free systems [11,18,21], opening many avenues for strategic applications of selectively...

Ngày tải lên: 07/03/2014, 12:20

... the more normal method of purifying proteins (save if the sole purpose of obtaining a sample was for sequencing), to think of another method of purification (normally used for a slightly different ... element of inventive step In Genentech/PF4A receptors21, the EPO Technical Board of Appeal considered that the use of a non-standard method for the isolation of receptors interacting with members of ... in applications for biotechnological inventions Examination Guidelines for Patent Applications relating to Biotechnological Inventions in the Intellectual Property Office Novelty Section of...

Ngày tải lên: 13/03/2014, 21:48

... levels of soluble APP remaining constant irrespective of the presence or absence of BACE1 The levels of APPsa increased to account for the loss of APPsb (soluble C-terminally truncated b-cleaved form ... the transcriptional properties of ICDs are contentious [45–48], there is consensus that the APP family of proteins may function as membrane anchors for a variety of proteins, and when CTFs are cleaved, ... C-termini of the different proteins The specificity of antibodies for their cognate target proteins was confirmed using brains from APP, APLP1 and APLP2 KO mice (Fig S1) In NaCl ⁄ Tris extracts of mouse...

Ngày tải lên: 15/03/2014, 10:20

... effect of transamination is a reduction of the degree of 15N-labelling in the amino acid of interest In the case of [15N]Gly, much of the label appeared to have been trapped in an amide group of ... predominantly N-formylated due to saturation of the activity of peptide deformylase (D Mouradov and T Huber, unpublished data) In the present case of hCypA, retention of the N-formyl group enables ... concentrations of T7 or E coli RNAP used in the present work were found to be suitable for production of many different proteins Ó FEBS 2004 Cell-free synthesis of 15 N-labelled proteins (Eur...

Ngày tải lên: 16/03/2014, 18:20

... sensitivity for the cofactor of the immobilized proteins is only possible with nanostructured Ag for direct excitation of surface plasmons down to 400 nm With the recent discovery of the coupling of surface ... spectroscopy: since the electronic transitions of most of the protein cofactors (e.g haem) of proteins are at shorter wavelengths, the combination of molecular RR and the surface-enhanced Raman ... materials for metal electrodes are monolayers and bilayers of lipid analogues, as they allow mimicking biological surfaces appropriate for protein binding [16] For soluble proteins, SAMs of x-functionalized...

Ngày tải lên: 22/03/2014, 16:20

... Most of the folds are assigned to one of the five structural classes on the basis of the secondary structures of which they composed: (1) all alpha (for proteins whose structure is essentially formed ... In Crystallographic Databases—Information Content, Software Systems, Scientific Applications (Allen, F H., Bergerhoff, G & Sievers, R., eds), pp 107–132, Commission of the International Union of ... The unit of classification is usually the protein domain Small proteins, and most of those of medium size, have a single domain and are, therefore, treated as a whole The domains in large proteins...

Ngày tải lên: 23/03/2014, 12:20

... along the centerline of the inksubstrate interface for 2.0 W of laser power when the center of the laser is located 90 ␮m from the beginning of scan The figure shows the region of the heated area ... temperature, is only of the order of milliseconds Also, the substrate near the interface is only above the damage threshold of soda-lime glass for less than 0.5 and 0.2 ms for scan speeds of 0.1 and 0.4 ... temperature profile for firing thick-film inks, of which QS300 is typical, requires heating the ink to 850 ° C at a ramp speed of FIG dc resistance in ⍀ for 10 mm wire after two layers of metallization...

Ngày tải lên: 06/05/2014, 08:54

... ability to reduce metals for which there is no reducing agent that can it For example, preparing metallic Mg nanoparticles.The last technique was named by us RAPET It stands for Reaction under Autogenic ... used in our laboratory for the last four years We will show that the RAPET of silanes is yielding the smallest reported SiC particles having the highest surface area The RAPET of empty Coca Cola ... The RAPET of empty Coca Cola bottles yields micron size carbon spheres with a tensile strength of 8.30±0.69 GPa ...

Ngày tải lên: 11/06/2014, 12:29

... samples were multiplied 50 times For calculations of the purification factor, we first calculated ratios of the viral proteins compared to the 88 kD and 44 kD cellular proteins in dpi medium and in ... dividing the intensity of the individual viral proteins in fractions 11–15 with the intensity of the proteins in the dpi medium We found that the recovery of the viral proteins was about 5% (Table ... we performed western blot analysis on samples balanced to each other on basis of the number of living cells at the end of collection We used a set of antibodies directed towards cellular proteins, ...

Ngày tải lên: 18/06/2014, 18:20

... resource of knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental techniques currently used for the study of human ... generic models of a human body with either fewer joints or reduced number of degrees of freedom are often utilized for enhancing computational performance For instance, existing methods for gait-based ... efficiency of troop movement During the 1950s there was a need for an improved understanding of locomotion for the treatment of World War II veterans The classic work at the University of California...

Ngày tải lên: 19/06/2014, 10:20