protein enzyme structure function and degradation
Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... Ionization Multi-Dimensional Protein Identification Technology N-terminal BAP and His tag N-terminal domain N-terminal FLAG and His tag N-terminal Protein A and CBP tag N-terminal S and CBP tag Reversed-phase ... approach The expression of proteins in E.coli and use of large expression tags like GST and maltose binding protein (MBP) may not be suitable for all eukaryotic proteins, and causes misfolding that ... use of IP and Co-IP is widely accepted as a robust means of demonstrating an actual protein- protein interaction, and is typically assessed by the specific detection of the protein target and bait...
Ngày tải lên: 11/09/2015, 09:05
Application of membrane technologies for biomolecules separation and purification by affinity binding to specific ligands
... (A) and near-UV CD spectra (B) for native, one time acid-denatured and one time base-renatured HSA Figure 3.6 Comparison of yields of L-tryptophan (A) and D-tryptophan (B) between native (●) and ... short testing time and high selectivity and sensitivity are compulsory In this regard, the chromatography and electrophoresis techniques are able to suffice such requirements and commonly selected ... f ,L where Cp,D and Cp,L are the concentrations of D-enantiomer and L-enantiomer in the permeate side, respectively, while Cf,D and Cf,L are the concentrations of D-enantiomer and L-enantiomer...
Ngày tải lên: 30/09/2015, 13:49
ELECTROSPUN AFFINITY MEMBRANE FOR IMMUNOGLOBULIN g PURIFICATION
... both Protein A and Protein G It is a gene fusion product secreted from a nonpathogenic form of Bacillus The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein ... towards designing and selecting ligands of high affinity and specificity [Figure 2.9] Ligands for protein affinity chromatography are distinguished into synthetic and biological The ligand-specific ... eliminated Protein A/G binds to all human IgG subclasses and it has a broader binding range than either Protein A or Protein G individually Although IgG purification kits utilizing protein A or protein...
Ngày tải lên: 05/10/2015, 19:04
bài tập lớn.doc
... int0 org 0013h ljmp int1 ; - MAIN PROGRAM org 30h main: MOV IE,#00000000B ; Enable int1 and int mov p2,#00h CLR A MOV MOV MOV MOV MOV l: A,#0ffh p2,A A,#066h B,#066h 7fh,#00h MOV R4,#077h ... int0 org 0013h ljmp int1 ; - MAIN PROGRAM org 30h main: MOV IE,#10000101B ; Enable int1 and int mov p2,#00h CLR A MOV A,#0ffh MOV p2,A MOV A,#066h MOV B,#066h MOV 7fh,#00h l: ACALL Forward...
Ngày tải lên: 04/08/2012, 14:26
Chiến lược marketing của tập đoàn hàng đầu việt nam unilever
Ngày tải lên: 08/08/2012, 10:48
