protein dscr 1 deters cancer and septic inflammation
Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt
... (55.9) CNS 1. 1 7868 (633) 0 .16 9 (0.266) 0 .19 0 (0.273) 0. 017 1. 8 27 1. 3 97 .1 2.9 17 .7/ 312 22.8/322 34.3/67 SHELX-97 20948 (19 24) 0 .11 9 (0. 217 ) 0 .18 5 (0.239) 0. 013 2.3 26 1. 8 96.8 3.2 18 .4/288 27.6/297 ... 17 –87 17 –87 17 –88 Water Resolution 38 67 67 10 2 10 2 12 7 3.0 2.0 1. 6 1. 6 1. 5 1. 5 1. 2 1. 2 1. 2 ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A R/Rfree 0.464 0.339/0.3 71 0 .19 0/0.224 0 .16 9/0 .19 0 0.409 0.375 0 .19 1/0.205 ... 98.5 (85 .1) 45.5 (5.3) 3.9 (24.6) R3 (hexagonal indexing) a ¼ b ¼ 74.96, c ¼ 33.23 NSRRC BL17B2 1. 0 717 RAXIS-IV+ + 83.4 1. 5 0.292 14 5 20 1. 2 (1. 24 1. 20) 14 1402 (11 874) 217 81 ( 210 9) 10 0.0 (99.8)...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
... Glu C peptides 83 -10 8 +2P 93 -10 0 +P 93 -10 3 +P 93 -10 3 +2P 93 -10 8 +P 10 0 -11 1 +P 10 9 -12 1 +P 10 9 -12 2 +P 11 2 -12 1 +P 11 3 -12 1 +P 16 1 -17 2 +P Tryptic peptides 16 5 -17 5 +P 16 5 -18 3 +P Sequence Calculated mass ... 16 + 25+ 24+ 22+ 13 + 14 + C 25 410 Da 21+ 20+ 19 + 18 + 23+ 25+ 15 + 17 + 16 + 15 + 13 + 14 + 24+ B 21+ 20+ 19 + 22+ 16 + 18 + 23+ 25+ 17 + 15 + 24+ 14 + 13 + A 10 00 12 00 253 31 Da 14 00 16 00 18 00 252 51 Da 2000 m/z ... 2787 .1 853.4 11 69.4 12 49.4 17 70.7 13 57.6 15 94.7 17 08.7 12 53.5 11 39.4 13 30.0 2787.8 853.2 11 69.3 12 49.2 17 71. 4 13 57.6 15 95.2 17 08.6 12 53.9 11 39.9 13 30.3 +0.7 )0.2 )0 .1 )0.2 )0.3 )0.5 )0 .1 +0.4...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx
... rerio, Drosophila melanogaster and Schizosaccharomyces pombe (GenBank accession nos XP_536855.2, Q8R3 31, NP_0 010 17056.2, NP_0 010 174 01. 1, NP_608895 .1 and NP_595833 .1) Residues identical in all compared ... repression by the FEBS Journal 274 (2007) 5370–53 81 ª 2007 The Authors Journal compilation ª 2007 FEBS 5379 ZNHIT -1 as a cofactor of Rev-erbb 10 11 12 13 14 15 16 J Wang et al orphan steroid receptor Rev-erbb ... dehydrogenase bifunctional enzyme [10 ], a-fetoprotein [11 ] and rat apoAI [12 ] genes Rev-erba is also involved in circadian timing in brain and liver tissue, and regulates Bmal1 which is the master regulator...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... 1. 20E-008 1. 28E-008 1. 00E-008 25 50 75 10 0 0 .10 0 0.095 0.090 0.085 1. 26E-008 AAAGACACG Kd=4 nM 0 .10 5 Kd =1. 2 nM 1/ ΔF340 1/ ΔF340 0 .11 0 Kd=0.29 nM 1. 32E-008 AAAGACATG 1. 40E-008 20 25 30 35 40 45 50 1/ [DNA]µM ... 32.5 ± 1. 8 (13 .5-fold) 23 .1 ± 1. 3 (2.5-fold) 90.2 ± 11 .1 (2.2-fold) 10 .8 ± (7.2-fold) P-values GFP–N-terminus of HIPPI (fold) P-values 0.00 01 12.5 ± 3.9 0.3 0.00 01 0.0002 0.0005 17 .6 ± 1. 9 (7.3-fold) ... Casp1ups 60 bp I C1ups 717 bp (HIPPI-pDED) C1ups 717 bp (HIPPI-Nterm) C1ups 60bp (HIPPI-pDED) 0.00 0. 21 II C1ups 60bp (6XHN-HIPPI_pDED) Kd=0.34nM 0.20 1/ ΔF Fluorescence305 B shifted band 0 .19 0 .18 ...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... Biomol Screen 10 , 12 7 13 6 Gether U & Kobilka BK (19 98) G protein- coupled receptors J Biol Chem 273, 17 979 17 982 Lefkowitz RJ (19 89) G protein- coupled receptors J Biol Chem 273, 18 677 18 680 Perfus-Barbeoch ... Genet 21, 414 – 419 15 Lochrie MA, Mendel JE, Sternberg PW & Simon MI (19 91) Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans Cell Regul 2, 13 5 15 4 16 Mendel ... Natl Acad Sci USA 10 3, 11 12 11 17 20 Miller KG, Emerson MD & Rand JB (19 99) Goa and diacylglycerol kinase negatively regulate the Gqa pathway in C elegans Neuron 24, 323–333 21 Hajdu-Cronin YM,...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... 13 05 13 98 14 62 14 62 14 62 13 98 11 22 10 13 II 13 05 11 22 13 05 I 13 98 + 14 62 11 22 13 05 10 13 + 13 98 13 05 11 22 13 98 14 62 14 62 10 13 11 22 13 05 13 98 14 62 + 14 62 13 98 11 22 11 22 10 13 13 05 62 13 98 14 62 13 05 ... GST 11 22 -13 04 13 05 -13 98 13 05 -14 62 10 13 99 -14 62 11 22 -14 62 GST-GRIP1 GST GST-GRIP1 Input 10 % B GRIP1 11 22 -14 62 GST-GRIP 113 05 -13 98 GRIP1 GST Input 10 % C 14 62 765 563 11 21 112 2 14 62 Fig GRIP1 forms ... 10 x AD1 11 22 Luciferase Activity B (RLU 10 ) 12 0 [Gal4DBD; pM vector] 563 11 21 AD1 10 13 AD1 Mr 14 62 AD2 10 16 0 1x 10 13 11 21 AD1 C 40 1x [Gal4DBD; pM vector] 35 858x 29000x Mr 10 55 40 10 0 72...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... (2005) 6 218 –6227 ª 2005 The Authors Journal compilation ª 2005 FEBS L.-P Chow et al A A newly identified pollen allergen as pathogenesis-related proteins 13 33 53 73 93 11 3 13 3 15 3 17 3 19 3 213 233 ... Purification and characterization of a novel isoallergen of a major Bermuda grass pollen allergen, Cyn d J Biomed Sci 10 , 11 1 11 9 10 Su SN, Lau GX, Yang SY, Shen HD, Tsai JJ & Han SH (19 90) Isolation and ... 402, 16 7 17 2 6226 L.-P Chow et al 17 Focke M, Hemmer W, Hayek B, Gotz M & Jarisch R (19 98) Identification of allergens in oilseed rape (Brassica napus) pollen Int Arch Allergy Immunol 11 7, 10 5 11 2...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... pACT2FEZ1 (Y156), pACT2FEZ1 (Y163), pACT2CIB (Y198) or pGAD424NBR1 and (i) pGBT9NBR1 (ii) pGBT9NBR1 216 (iii) pGBT9NBR1333 (iv) pGBT9NBR1C term (v) pGBT9NBR1c1 (vi) pGBT9NBR1c2 (vii) pGBT9NBR1c3, ... Parker, Ó FEBS 2002 10 11 12 13 14 15 16 17 18 19 L., Zurawski, V.R Jr & Knapp, R.C (19 83) A radioimmunoassay using a monoclonal antibody to monitor the course of epithelial ovarian cancer New Engl ... con®rm that NBR1 and FEZ1 interact in vivo COS-7 cells (lane 1) , COS-7 cells transfected with pHA-NBR1 (lane 2), pFEZ1-EGFP (lane 3), pHA-NBR1 and pFEZ1-EGFP (lane 4) and pHA-NBR1 and pEGFP-N2...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
... (19 98) Nuclear detection of cellular retinoic acid binding proteins I and 3570 R.-Z Liu et al 15 16 17 18 19 20 21 22 23 24 25 26 27 28 II with new antibodies J Histochem Cytochem 46, 11 03 11 11 ... Genetics 15 1, 15 31 15 45 46 Lynch M & Conery JS (2000) The evolutionary fate and consequences of duplicate genes Science 290, 11 51 11 55 47 Ruberte E, Friederich V, Chambon P & Morriss-Kay G (19 93) ... Development 11 3, 11 59 11 70 Colbert MC, Rubin WW, Linney E & LaMantia AS (19 95) Retinoid signaling and the generation of regional FEBS Journal 272 (2005) 35 61 35 71 ª 2005 FEBS R.-Z Liu et al 29 30 31 32...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Inhibitors of protein phosphatase 1 and 2A decrease the level of tubulin carboxypeptidase activity associated with microtubules pptx
... 3, 11 41 11 54 Sloboda, R.D., Rudolph, S.A., Rosenbaum, J.L & Greengard, P (19 75) Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein Proc Natl Acad Sci USA 12 , 17 7 18 1 ... J Cell Biol 10 5, 2 51 264 Schulze, E., Asai, D.J., Bulinski, J.C & Kirschner, M (19 87) Posttranslational modification and microtubule stability J Cell Biol 10 5, 216 7– 217 7 Kreis, T.E (19 87) Microtubules ... Bulinski, J.C & DeBrabander, M (19 86) Ultrastructural colocalization of tyrosinated and detyrosinated alpha-tubulin in interphase and mitotic cells J Cell Biol 10 3, 18 83 18 93 ´ 10 Bre, M.H., Kreis,...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot
... lanes 9, 10 , 11 and 12 ), albeit with different affinities SAFB1 binds tightly to SRPK1a and almost as tightly to SRPK1 (compare lanes and 1) and the same holds true for the GST–SAFB1CDRE protein ... al SRPK1/1a inhibition by interaction with SAFB1/2 Input GST– GST– GST– SAFB1C SAFB1CΔRE SAFB2C GST Anti-GST FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a Eluate ... + GFP + – – + – – GFP–SAFB1 – + – – + – GFP–SAFB2 – – + – – + 12 – FLAG–SRPK1a 11 + FLAG–SRPK1 Fig The binding effect of full-length SAFB1 and SAFB2 on SRPK1 and SRPK1a kinase activities HeLa...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents pdf
... 30.6 kDa (Fig 1B) The PPP1R3G cDNA sequence 14 79 Novel glycogen-targeting subunit of PP1 A S Munro et al Gene -272 417 719 E1 12 79 17 13 18 19 E2 3036 317 6 E3 3660 E4 6656 E5 mRNA (ATG) E1 837 (STOP) ... similarities to the human genomic sequences PPP1R3E, PPP1R3F and PPP1R3G (Accession nos: AL049829, NM_033 215 , AL035653) with chromosomes locations 14 q 11. 2, Xp 11. 23 and 6p24.3-25.3, respectively In order ... the same enzyme: protein phosphatase -1 Eur J Biochem 15 6, 10 1 11 0 Bollen M, Keppens S & Stalmans W (19 98) Specific features of glycogen metabolism in the liver Biochem J 336, 19 – 31 Armstrong CG,...
Ngày tải lên: 30/03/2014, 16:20
báo cáo hóa học:" RAGE (Receptor for Advanced Glycation Endproducts), RAGE Ligands, and their role in Cancer and Inflammation" pptx
... receptor for Page 19 of 21 (page number not for citation purposes) Journal of Translational Medicine 2009, 7 :17 14 5 14 6 14 7 14 8 14 9 15 0 15 1 15 2 15 3 15 4 15 5 15 6 15 7 15 8 15 9 16 0 16 1 16 2 advanced glycation ... http://www.translational-medicine.com/content/7 /1/ 17 10 11 12 13 14 15 16 Acknowledgements This report was funded, in part, by PO1 CA 10 1944-01A2 (Lotze, Michael T) Integrating NK and DC into Cancer Therapy and under a special ... Plasma Proteome and Peptidome Clin Chem 2006, 52 :12 23 -12 37 http://www.translational-medicine.com/content/7 /1/ 17 86 87 88 89 90 91 92 93 94 95 96 97 98 99 10 0 10 1 10 2 10 3 10 4 10 5 10 6 Zimmerman SB,...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo sinh học: "Time-course of sFlt-1 and VEGF-A release in neutropenic patients with sepsis and septic shock: a prospective study" pptx
... factor is an important determinant of sepsis morbidity and mortality J Exp Med 2006, 203 :14 47 -14 58 Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Shapiro N, Schuetz P, Yano K, Sorasaki ... http://www.translational-medicine.com/content/9 /1/ 23 Table Diagnostic accuracy of sFlt -1 and VEGF-A levels for septic shock development Biomarker Time-point sFlt -1 VEGF-A 0. 61 (0. 41- 0. 81) ; P = 0. 81 0.58 (0.38-0.77); P = 0.47 0.87 (0.73 -1. 00); ... neutropenia (median, range) 12 (4 - 22) 14 (7 - 30) 0.36 * Platelets 10 3/μl - fever (median, range) 25 (6 - 16 9) 38 (12 - 90) 0 .16 * Days with fever (median, range) (1 - 12 ) (1 - 12 ) 0.65 * SOFA score...
Ngày tải lên: 18/06/2014, 19:20
Báo cáo sinh học: "Protein kinase CK2a is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes" docx
... http://www.translational-medicine.com/content/9 /1/ 97 Cell lines and culture conditions The human colorectal cancer cell lines LoVo, SW480, HT29, HCT 116 and LS174T were maintained in RPMI 16 40 (Gibco, Grand Island, NY, USA) supplemented ... N1-2 49 23 (46.9) 26 (53 .1) M0 60 26 (43.3) 34 (56.7) 0.897 Tumor location 0.554 T stage T1-T2 T3-T4 0.002* N stage 0. 515 M stage 0.632 M1 44 17 (38.6) 27 ( 61. 4) TNM stage I-II 30 11 (36.7) 19 ... Medicine 2 011 , 9:97 http://www.translational-medicine.com/content/9 /1/ 97 Page 11 of 11 Unit and Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510 515 , Guangdong...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Time-course of sFlt-1 and VEGF-A release in neutropenic patients with sepsis and septic shock: a prospective study" pdf
... factor is an important determinant of sepsis morbidity and mortality J Exp Med 2006, 203 :14 47 -14 58 Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Shapiro N, Schuetz P, Yano K, Sorasaki ... http://www.translational-medicine.com/content/9 /1/ 23 Table Diagnostic accuracy of sFlt -1 and VEGF-A levels for septic shock development Biomarker Time-point sFlt -1 VEGF-A 0. 61 (0. 41- 0. 81) ; P = 0. 81 0.58 (0.38-0.77); P = 0.47 0.87 (0.73 -1. 00); ... neutropenia (median, range) 12 (4 - 22) 14 (7 - 30) 0.36 * Platelets 10 3/μl - fever (median, range) 25 (6 - 16 9) 38 (12 - 90) 0 .16 * Days with fever (median, range) (1 - 12 ) (1 - 12 ) 0.65 * SOFA score...
Ngày tải lên: 20/06/2014, 03:20
báo cáo khoa học: "Clinical significance of preoperative serum interleukin-6 and C-reactive protein level in breast cancer patients" pot
... Tumor depth pT1 8 .1 ± 5.7 pT2 17 .8 ± 8.6 0.564 0.0 01 8.4 ± 3 .1 pT3 19 .2 ± 10 .0 9.0 ± 2.4 pT4 26.2 ± 11 .2 0.374 0.304 16 .0 ± 3.3 10 .5 ± 2.7 LN meta N1 11 .6 ± 4.8 N2 20.7 ± 6.9 0.844 0.000 15 .7 ± 9.0 ... Median(Range) 59 .11 (36-85) Depth of tumor invasion pT1 11 .9 pT2 15 25.4 pT3 14 23.7 pT4 23 38.9 LN metastasis N0 8.6 N1 17 28.8 N2 21 35.6 N3 16 27 .1 Metastasis(-) 51 86.4 Metastasis(+) Page of 13 .6 Distant ... Cancer 19 91, 64 :11 33 -11 38 12 Bloom HJG, Richardson WW: Histological grading and prognosis in breast cancer: a study of 14 09 cases of which 539 have been followed up for 15 years Br J Cancer 19 57,...
Ngày tải lên: 09/08/2014, 01:24
Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx
... 2003, 523-524 :14 5 -15 0 45 Gusman J, Malonne H, Atassi G: A reappraisal of the potential chemopreventive and chemotherapeutic properties of resveratrol Carcinogenesis 20 01, 22 :11 11- 111 7 46 Kundu ... 0, 12 , 24, and 48 hours with 10 ng/ml IL -1 or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12 , 24, and 48 hours stimulation with 10 ng/ml IL -1 Equal amounts (500 ng protein ... treated either with 10 ng/ml IL -1 5, 15 and 30 minutes alone or pre-treated with 50 μM resveratrol and 50 μM curcumin for hours and then co-treated with 10 ng/ml IL -1 for 5, 15 and 30 minutes,...
Ngày tải lên: 09/08/2014, 14:22
báo cáo khoa học: "EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pptx
... Male 16 15 P value negative 40.50% Ages 0.448 Sex 0.445 Female 11 42 .10 % Pathologic type Squamous carcinoma 13 40% Adencarcinoma 13 13 50% Mixed type 66.70% ≤3 cm 43.80% >3 cm 18 16 47 .10 % 21 19 ... non-small cell lung cancer to long term survival Br J Cancer 19 93, 68 :16 2 -16 5 30 Nicholson RI, Gee JMW, Haper ME: EGFR and cancer prognosis European Journal of Cancer 20 01, 37(4):9 -15 31 Van Dyke AL, ... >60 15 88.20% Sex Male 27 87 .10 % Female 18 94.70% Squamous carcinoma 16 76.20% Adencarcinoma 26 10 0% 10 0% 0.362 Pathologic type Mixed type 0.022* Tumor length ≤3 cm >3 cm 0. 518 14 31 87.50% 91. 20%...
Ngày tải lên: 10/08/2014, 10:21