promoters terminators selectable genes and reporter genes

... hairy hand reached out from the darkness and plucked him off his feet The hand slammed him against the remains of a brick wall, making him gasp out loud Moni flinched, as a burning brand was ... In every city there were hidden rooms like this Places to storearms and food Places to meet and talk and plan Places where men and women met with one burning desire in common—to take back their ... just bare gleaming metal walls and floor, and a plain functional desk But to the Controller himself it was evidence of his power and rank Few humans enjoyed such space and luxury But after all, he...

Ngày tải lên: 30/05/2014, 22:53

... difference between the RTP in promoters and that in non -promoters, and FDHexamer1 and FDHexamer2 were the frequency differences of two candidate hexamers in promoters and nonpromoters for the given ... discrimination power of the two candidate hexamers (candidate_hexamer1 and candidate_hexamer2) could then be measured by the difference of the frequency between promoters and non -promoters The diff_RTP_hexamer ... used for testing Each set of training data contained 244 promoters and 244 non -promoters, and each test data had 61 promoters and 61 non -promoters Plant promoter sequence database FDAFSA For this...

Ngày tải lên: 12/08/2014, 17:20

... “love God and your neighbor and you will fine”) that dismisses as accommodation and potentially erroneous the communicator’s genre-encased message The authority and inerrancy of the text is, and has ... John 1:3 and Colossians 1:16, not Genesis In both 34 The Lost Wor ld of A da m and Eve of these New Testament passages, the emphasis is on the authority and status of the Son of God and not on ... local landmarks and normal traffic times between them for out-of-town listeners or inexperienced commuters These would be much longer reports If the traffic reporter made the report understandable...

Ngày tải lên: 08/06/2015, 17:26

... problems that Marx has bequeathed to us, and with the false solutions he brought to them class-initself and class-for-itself, working class and proletariat, and so on that one must not be afraid ... occupy similar positions and who, being placed in similar conditions and subjected to similar conditionings, have every likelihood of having similar dispositions and interests and therefore of producing ... according to Emile Benveniste, t o regerefines and regere sacra, t o set forth the frontier between the sacred and the profane, good and evil, the vulgar and the distinguished If sociologists are...

Ngày tải lên: 16/02/2016, 09:33

... identifying orchid genes and promoters - in particular the genome walking method - cannot easily be performed when regions of high DNA sequence homology tandem repeats and tandemly duplicated genes are ... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 ... OnTI, OnExpansin and Oncidium MADS genes contain nucleotide tandem repeat sequences (Table 3) However, promoter studies demonstrated that the tandem repeats in OnTI1 and OnExpansin promoters are...

Ngày tải lên: 11/08/2014, 11:21

... digital expression analyses and RT-PCR suggest that the Ppbec1, Ppxero2 and Pptha1 are cold-inducible genes The promoters of these cold-inducible genes were isolated and characterized using both ... isolating and functionally characterizing promoters of cold-inducible peach genes To date, only a few inducible promoters have been identified in crop plants The Pptha1, Ppbec1 and Ppxero2 genes ... Ppxero2 (DX-82F and DX176R), thaumatin Pptha1 (THA30F and THA382R), lipoxygenase Pplox1 (LOX982F and LOX1267R) and the actin Ppact7 (ACT-F and ACT-R) genes are shown in Table Primers used to amplify...

Ngày tải lên: 12/08/2014, 03:21

... transcripts and small RNAs, L2 and L3 promoters, microarray probes, TFBS predictions and ChIP-chip signal for PU.1, SP1, and acetylated H3K9 and enable users to relate CAGE signal to alternative promoters ... of genes and miRNAs For example, given a set of candidate genes (for example, genes mutated in leukemia or co-regulated in a microarray experiment), what are the predicted edges between them and ... by deepCAGE and microarray), regulatory inputs (top left), gene annotation and protein-protein interactions (top middle), and the regulatory targets for transcription factor genes and miRNAs as...

Ngày tải lên: 14/08/2014, 21:20

... on fives genes involved in transcriptional and translational processes As shown in Table 1, the up-regulated genes were COBRA1, ITGB4, STAU2, and HMGN3 with a SLR of 3.30, 2.61, 1.68 and 1.52, ... interactions of each of these genes ITGB4 and ANK3 are associated with different targets, including small molecules, genes and proteins In contrast, COBRA1, STAU2 and HMGN3 are only associated ... induction of transcription factors (AP1, Elk1, Stat1, SRF and NFκB) and expression of apoptotic genes (Fas receptor, Fas ligand, FADD and caspase 8) [19] In addition, as confirmed by the functional...

Ngày tải lên: 31/10/2012, 15:28

... complex interactions between genes and the social environment Understanding their implications and the new biology they describe are key goals for the behavioural sciences and for psychiatry Devising ... children and the effect of genotype was assessed on 14 measures of cognitive function, including working memory, verbal and motor inhibition, attentional control, and IQ, assessed at ages and 10 ... interactions in schizophrenia and other disorders One serious issue in these studies will be the specificity of the genetic and environmental risks The search for candidate genes for schizophrenia...

Ngày tải lên: 01/11/2013, 09:20

... Testis Ovary Testis Embryogenesis, somites, pharyngeal arches and brain Embryogenesis Olfactory placodes, neural tube Spinal cord Embryogenesis, brain Embryogenesis, nasal and otic placodes, telencephalon, ... induction and maintenance, illuminating its important function But what about the other dmrt genes? Table summarizes the expression pattern of these genes in fish during development and in the ... to male gonadogenesis and further differentiation (Table 1) Dmrt1 expression in protogynous and protandrous hermaphroditic species In hermaphrodite fish (protogynous or protandrous), the developmental...

Ngày tải lên: 14/02/2014, 19:20

... CANCER, GENES AND INHERITED PREDISPOSITION Overview – Cancer Genetics ‘Cancer protection’ genes Since the growth control genes normally provide information to prevent ... much accelerator) • Mismatch repair genes • These are genes that are involved in finding and repairing mistakes in the genetic information that can occur when the genes are copied to make new cells ... to make the car move (the accelerator or oncogenes) and stop (the brakes or tumour suppressor genes) must be in balance Also the mismatch repair genes are like the mechanic who keeps the car...

Ngày tải lên: 15/02/2014, 04:20

... 5¢-CTCGAGCCACCCAATTTCGGAAAGT Table Over-expression plasmids for Sno1p and Snz1p prepared in this study Symbols O, Z and H stand for Sno1p, Snz1p and His-tag, respectively Primer Name of plasmid Forward Backward ... pyridoxine and leucine The plasmids were isolated from S cerevisiae as described [14] Construction of over-expression plasmids for the SNO1 and SNZ1 genes The coding regions of the SNO1 (672 bp) and ... found that 4.6 and 5.1 kb overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementing the defect of mutant K64 (Figs and 2) They are homologues of PDX2 and PDX1 of...

Ngày tải lên: 19/02/2014, 12:20

... homology with other CKX genes To amplify full-length genes, and to distinguish between HvCKX2 and HvCKX3 genes, one sense primer (CKX25, 5¢-CAGTGAACCACTACCCTGCTACA CG-3¢) and two antisense primers ... Hordeum and Triticum, as well as report on the cloning of the first two CKX genes of barley and demonstrate functionality for one of them in transgenic tobacco and Arabidopsis plants Materials and ... HvCKX genes with HvCKX2r, HvCKX3r, and HvCKX23f primers Amplified DNA was cloned into the pDRIVE vector and sequenced The same primers, with Asp718 and XbaI overhangs, were used to reamplify both genes...

Ngày tải lên: 19/02/2014, 16:20

... PrP1 and human PrPC, 62% between zebrafish PrP1 and PrP2, 66% between zebrafish PrP1 and Fugu PrP1, and 57% between zebrafish PrP1 and Fugu PrP2 sequences (Fig 2) Conserved sequence motifs and amino ... stPrP-1 and stPrP-2 [10,11] and renamed in this study PrP1 and PrP2, respectively Given that the zebrafish PrP1 sequence could be aligned in its entirety with zebrafish PrP2, Fugu PrP1 and PrP2, and ... correlate with the time and sites of PrP1 and PrP2 action(s) during nervous system morphogenesis and also suggest that PrP2 has a pleiotropic role in the course of embryogenesis A high expression...

Ngày tải lên: 19/02/2014, 16:20

... is bound to both LMO2 and HGAL promoters and thus can directly act to suppress these genes PRDM1 directly regulates the HGAL and LMO2 promoters PRDM1 regulates its target genes at the level of ... sites in the LMO2 and HGAL promoters are functional sites of PRDM1 mediated repression Discussion This study demonstrates that PRDM1 regulates the GC and GC-DLBCL marker genes, HGAL and LMO2 The repression ... important to define the role of PRDM1 inhibition of HGAL and LMO2 in the pathogenesis and outcome of DLBCL Materials and methods Cell lines and protein accession numbers Human non-Hodgkin lymphoma...

Ngày tải lên: 05/03/2014, 23:20

... different Hsp genes in D melanogaster and whether or not these Hsps have overlapping activities remain open questions The use of mutant (e.g deletion and extra copy numbers) and transgenic (RNAi and overexpression) ... al Other Hsp genes The mRNA levels of Hsp60, Hsp67Ba and Hsc70-1 did not show any signicant modulation relative to controls during cold stress and during recovery periods (Table and Fig 3) In ... freedom = 11,30; P = 0.766) Hsp70 and Hsp68 Of the 11 Hsp genes examined, Hsp70Aa and Hsp68 were the most cold-inducible, with overall expression being treatment-specic, and expression being upregulated...

Ngày tải lên: 06/03/2014, 09:22

... IAA, 13 ARF and 12 SAUR genes, were differentially expressed in at least one stage of panicle development, and 10 genes, including one GH3 gene, five Aux ⁄ IAA genes, three ARF genes and one SAUR ... auxinresponsive genes revealed that, among a total of 84 genes that were differentially expressed, 48 (44 auxininduced and four auxin-repressed) genes were upregulated and 36 (all of them auxin-induced) genes ... SAM, and various developmental stages of panicle (P1-I– P1-III and P1–P6) and seed (S1–S5) Various stages of rice panicle and seed development have been categorized according to panicle length and...

Ngày tải lên: 07/03/2014, 01:20

... the antiparallel genes are translated, and to study the involvement of dmdR1 and the antisense gene (adm) in the regulation of S coelicolor A(3)2 sporulation and antibiotic and siderophore biosynthesis ... formation of desferrioxamines B and E (labelled as DesB and DesE) and complementation of desferrioxamine production (A) Cultures of S coelicolor A3(2) and the TAdmdR1, TAadm and DdmdR1 ⁄ adm mutants ... antiparallel genes and iron regulation A B C Fig Immunodetection of DmdR1 and Adm proteins (A,B) Western blot reactions of wild-type S coelicolor A3(2) (lane 2) and the TAadm (lane 3), TAdmdR1 (lane 4) and...

Ngày tải lên: 07/03/2014, 02:20

... +), 731 genes were regulated by leucine starvation: 420 genes were up-regulated by more than 1.8-fold and 311 genes were down-regulated by more than 1.8-fold (Fig and Table S1) These genes were ... that 622 genes were regulated by rapamycin treatment: 444 genes were up-regulated by more than 1.8-fold, and 178 genes were down-regulated by more than 1.8-fold (Fig 4A and Table S2) These genes ... expressing or not expressing GCN2 (GCN2+ ⁄ + and GCN2) ⁄ ) cells) In GCN2) ⁄ ) cells, 108 genes were regulated by amino acid starvation: 88 genes were induced and 20 genes were repressed by more than 1.8-fold...

Ngày tải lên: 07/03/2014, 03:20

... two genes The fragments were sequenced and enabled the complete reconstruction of the Psy1 and Psy2 genes with the annotation of introns and exons The GenBank accession numbers of the two genes ... of Psy1 and Psy2 genes and the transcription patterns of both genes in different tomato tissues The role of carotenoids as secondary metabolites in the pigmentation of tomato flowers and fruits ... structure The sequenced regions of the two genes were 4527 and 3542 bp long, respectively, for Psy1 and Psy2 The two genes contain at least seven exons and six introns The data are not conclusive...

Ngày tải lên: 07/03/2014, 05:20