proliferation apoptosis and differentiation

... – Apoptosis and Necrosis Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Apoptosis Assay Methods Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Methods for studying apoptosis ... studying apoptosis in individual cells 38 39 Cell Death – Apoptosis and Necrosis Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Apoptosis Assay Methods Cell Death – Apoptosis and Necrosis ... ̆ Table 1: Differential features and significance of necrosis and apoptosis Cell Death – Apoptosis and Necrosis Introduction Differences between necrosis and apoptosis b Prelytic DNA fragmentation...

Ngày tải lên: 10/04/2014, 11:02

... contributions YZ and AY are researchers working in cancer biology and carried the study QL and YB undertook the Statistical analysis QZ along with MZ designed the work and interpreted the results QZ and MZ ... arsenic trioxide on early and late apoptosis/ necrosis of SGC7901 cell (* represents p < 0.05 compared to the black control group and arsenic trioxide treated groups) at 24 hours and the difference compared ... western blot and stained by both anti-Bcl-2 (BH3) and anti-Bax (6A7) antibodies, express conformational change of Bcl-2, which may play a role in arsenic trioxide-induced apoptosis and Bax activation...

Ngày tải lên: 09/08/2014, 03:21

... stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The added serum contains high levels of growth factors released by cells and platelets ... The cells from the unaffected and affected sides expanded with, on average, 0.21 and 0.22 cell doublings per day, respectively After one passage (4.3 cell doublings) and weeks of culture, 106 primary ... implant has to withstand mechanical stress is that systematic redifferentiation signalling, as part of the disease condition, could be impaired within the OA joint Redifferentiation and proteoglycan...

Ngày tải lên: 09/08/2014, 06:22

... hypertrophy and normal cardiac function A, Representative transthoracic M-mode echocardiogram for sham and TAC mice B, IVSd, LVPW, and LVFS data between sham and TAC mice Cardiomyocyte Proliferation ... DCM and ICM VEGF treatment maintained mitochondrial fission and fusion balance, increased the expression of inhibiting apoptosis proteins OPA1 and Bcl-xL, decreased the expression of promoting apoptosis ... and recommence proliferation Additionally VEGF may give rise to reduce apoptosis of viable CMs, thereby contributing to CMs proliferation However, the precise mechanisms of VEGF-induced CMs proliferation...

Ngày tải lên: 10/08/2014, 09:21

... modules and sequence of events that leads from initial apoptosis to proliferation of apoptosis- resistant endothelial cells The combination of initial apoptosis induced by VEGF receptor blockade and ... design and conception and drafted the manuscript KT drafted the manuscript and contributed to its design and conception NV contributed to its design and drafted the manuscript All authors read and ... endothelial cell initial apoptosis to proliferation of apoptosis- resistant endothelial cells and vascular smooth muscle cells and endothlial-mesenchymal (SM-like) transdifferentiation Apoptotic...

Ngày tải lên: 12/08/2014, 14:20

... mixed and left to stand at room temperature for 5-10 minutes The organic and aqueous phases were separated by adding 240μl of chloroform and 240 μl of 2N KCl The mixture was then vortexed, and ... concentrations tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement with previous findings ... (10μM, 25μM, 50μM, 75μM, and 100μM) tested 54 Chapter2 Development and Evaluation of Human SPHK Inhibitors * * Figure 2.8 Effects of DMS and compounds on DAGK activity DMS and six compounds (CP1-CP6)...

Ngày tải lên: 11/09/2015, 16:06

... BM-MSCs and AD-MSCs differentiation, and the non-specific SPHK inhibitor, DMS, was used as a control Two differentiation pathways were chosen to study, namely, osteogenic and adipogenic differentiation, ... the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain differentiation pathway(s) This hypothesis ... characterized pathways in human BM- and AD- MSCs differentiation 65 Chapter Roles of SPHK Inhibitors in Human BM- and AD- MSCs Differentiation 3.1 MATERIALS AND METHODS All chemicals, if not specially...

Ngày tải lên: 11/09/2015, 16:06

... human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM -and AD- MSCs were cultured in different conditions for days, and cell ... BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were tested by PicoGreen assay Results are the average + the standard ... Chapter Role of S1P in human BM- and AD- MSCs proliferation four days, and human AD-MSCs were also cultured for eight days in different culture conditions, and the cell proliferation was detected by...

Ngày tải lên: 11/09/2015, 16:06

... osteogenic differentiation and adipogenic differentiation in human MSCs differently In human BM- and AD- MSCs osteogenic differentiation, 0.5μM CP6 and DMS showed to promote the osteogenic differentiation ... could be expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles ... osteogenic and adipogenic differentiation switching To our knowledge, this is the first report on inhibitors of SPHK mediating human BMand AD- MSCs differentiation into osteogenic and adipogenic...

Ngày tải lên: 11/09/2015, 16:06

... two psoriasis susceptibility loci (HLA and 17q) and two novel candidate regions (16q and 20p) by genome-wide scan." Hum Mol Genet 6(8): 1349-56 Nirmalanandhan, V S., M Rao, et al (2007) "Effect ... sphingosine-1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS ... poly(ADP-ribose) polymerase and lamins during Fas- and ceramide-mediated apoptosis in Jurkat T lymphocytes." J Biol Chem 273(5): 2910-6 Dekel, B., S Metsuyanim, et al (2006) "Multiple imprinted and stemness...

Ngày tải lên: 11/09/2015, 16:06

... stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation More details about the research goals and strategies ... expansion and differentiation are addressed, including their advantages and their potential shortcomings 1.4.5 Current Strategies in MSCs Proliferation and Differentiation 1.4.5.1 Serum and Growth ... pathogenic processes as discussed above, recently, SPHK and S1P have been suggested to be involved in stem cell proliferation and differentiation 1.3 SPHK AND S1P REGULATION IN STEM CELL RESEARCH As was...

Ngày tải lên: 11/09/2015, 16:06

... proliferation, induction of regulatory T cells (Treg cells), steering monocytes and mature DC cells to an immature DC state, inhibition of B cell proliferation and terminal differentiation, and ... cell apoptosis and cell proliferation Apoptosis, programmed cell death, plays an essential role in the process of boy health development and maintenance by eliminating the old, unnecessary, and ... unfolding and secretion (Mkrtchian and Sandalova, 2006) 1.4.1 Structure and Distribution The human ERp29 gene is located at chromosome 12q24.13 and contains three small exons separated by one small and...

Ngày tải lên: 02/10/2015, 17:15

... 1,25-(OH)2D3 on podocyte apoptosis and podocytopenia The present study aimed to investigate the effects of 1,25-(OH)2D3 on podocyte apoptosis and podocytopenia induced by adriamycin (ADR) and the potential ... Bax/Bcl-2 ratio and finally limiting glomerular cell apoptosis Our study demonstrates the protective effect of 1,25-(OH)2D3 on podocyte apoptosis by down-regulating Fas, FADD and Bax and up-regulating ... collagenase and culture Early cellular outgrowths at 5-7 days were selectively removed by a cylinder cloning technique Cells were replated, and plates growing pure colonies were then expanded and identified...

Ngày tải lên: 25/10/2012, 11:40

... of TJ proteins such as claudin-1 and claudin-4 induces apoptosis and suppresses metastatic potencies [10,11] Occludin has also been reported to induce apoptosis and apoptotic sensitization, which ... occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the reduction ... concentration increases apoptosis and decreases invasiveness On the basis of the differences between OccWT and OccDE9 with regard to mitochondria-dependent apoptosis and reduction of invasion,...

Ngày tải lên: 18/02/2014, 18:20

... microvilli and junctional complexes, and blebbing can occur In several cases, an intense endocytosis is observed, and this probably Fig Multiple pathways to apoptosis, both dependent and independent ... activation (GluRd2-Lc) Yue and collaborators [113] showed that GluRd2, nPIST and beclin-1 interact, and that autophagy can be induced by nPIST and beclin-1 synergy and by the mutated GluRd2-Lc, ... section will examine its relationship with apoptosis Apoptosis vs autophagy Notwithstanding the abundant evidence for a role for both autophagy and apoptosis in various diseases, their interplay...

Ngày tải lên: 07/03/2014, 15:20

... associated with reduced tumour proliferation, and tumour regression, accompanied by apoptosis [40] An understanding of how and under what circumstances MEK inhibition can promote apoptosis may permit a ... variants, BIMS, BIML and BIMEL (BIM-short, BIM-long and BIM-extra long, respectively), are all cytotoxic and subject to different modes of regulation by various prodeath and prosurvival signalling ... loss of ERK1 ⁄ and ⁄ or PKB signalling and, as a consequence, promote increased expression of BIM and BIM-dependent cell death in tumour cells Here we review recent advances in understanding BIM...

Ngày tải lên: 16/03/2014, 00:20

... kinases ATM and ATR ATR and ATM in turn phosphorylation-dependently activate the downstream checkpoint kinases Chk1 and Chk2, respectively, and the tumour suppressor p53 Furthermore, ATM and ATR ... we focus on the apoptotic p53, p73 and HIPK2 in apoptosis signalling routes of the DDR regulated mainly from the cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein ... cisplatin) and has been shown to drive the expression of apoptotic p53 target genes, such as p53-upregulated modulator of apoptosis (Puma), p53 regulated apoptosis- inducing protein (p53AIP1) and breakpoint...

Ngày tải lên: 16/03/2014, 00:20

... caspase-1, caspase-9 ⁄ HIP-1 & HIPPI mediated apoptosis and transcription caspase-6, and caspase-3 Cleavage of Bid and release of cytochrome c and apoptosis- inducing factors from the mitochondria ... apoptosis and transcription Hippi Also, expression of the mitochondrial-coded genes ND1 and ND4, the nuclear genome-coded mitochondrial genes SDHA and SDHB and the antiapoptotic genes BCL-2 and ... in the regulation of cytoskeletal and endocytic processes HIP-1 and its interacting partners – roles in apoptosis and survival Various pathways followed during apoptosis have been reviewed recently...

Ngày tải lên: 23/03/2014, 07:20

... abundant in placenta, AHR [50], AP-2 and PPARc; (i) proliferation and/ or apoptosis regulators, AP-2, c-MYC [51] and NF-jB; (j) embryo- and organogenic factors, PAX-2 and PAX-5; (k) proto-oncogenes ... transcription factor binding sites involved in apoptosis and differentiation were localized in the PP17 gene promoter Using well-characterized apoptosis and differentiation models, induction of PP17 ... from milk and its cDNA was isolated from a mammary gland clone collection [57], while human mammary gland and mammary adenocarcinoma ESTs similar to PP17b cDNA was found by BLAST, and subsequently...

Ngày tải lên: 23/03/2014, 21:20

... the androgen receptor to control cellular processes such as proliferation, apoptosis and other metabolic events in prostate cancer [14] Taken together, a combination of gene amplification and androgen ... functions such as adhesion, migration, differentiation, proliferation, survival and motility [37] Usually, integrins avb3 and avb5 are involved in cell migration and attachment to the extracellular ... Fig Influence of TPD52 overexpression on the proliferation of the PCA cell line LNCaP (A) Cell proliferation after overexpression of TPD52 and (B) cell proliferation after downregulation of TPD52...

Ngày tải lên: 30/03/2014, 02:20