projectoriented and functional organizational structures

Ngày tải lên: 11/11/2013, 16:40

Ngày tải lên: 17/03/2014, 11:20

Ngày tải lên: 22/03/2014, 15:21

... Copyright Computers and Structures, Inc., 1978-1997. The CSI Logo is a registered trademark of Computers and Structures, Inc. SAP2000 is a registered trademark of Computers and Structures, Inc. Windows ... the frame elements and joints. ã Click and hold the left mouse button. ã While holding, move the pointer below and to the right of the frame elements and joints. A “rubber-band” window will show ... Computers and Structures, Inc. Berkeley, California, USA Issue Date: June 1998 Revision Number : 0 Revision Date: N/A SAP2000  Integrated Finite Elements Analysis and Design of Structures SAP2000...

Ngày tải lên: 06/09/2012, 15:56

Ngày tải lên: 19/12/2013, 13:49

... improvement of ship and offshore installation safety standards, design standards and management. Accidents are the driving force of better safety and environmental protection standards. Ultimate ... safety culture and development of ship and offshore installations design standards. 2 ACCIDENT AND CASUALTIES - THE DRIVING FORCE TO IMPROVE SAFETY AND ENVIRONMENT PROTECTION STANDARDS In ... opposite that the inflation and interest rates are relatively low worldwide, government deficits significantly reduced, and the environment of domestic demand and external sale quite relaxed....

Ngày tải lên: 13/02/2014, 16:20

... extremities were carried out, using standard procedures. These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full). ... illness. Best performance was in "standing from chair without using arms" (84%), semi tandem stand was completed by 78% and full tandem stand by 72%. The poorest performance was ... (IADL)" and included ability to travel outside, go shopping, prepare own meals and handle money. A limited number of tests of physical performance for assessing the functioning of upper and...

Ngày tải lên: 14/02/2014, 06:20

... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... Environment, Agricultural Sciences and Spatial Planning (to L.W. and S.E.), the Swedish Research Council (to H.E. and P.N.) and the Swedish Cancer Foundation (to H.E. and P.N.). The Structural Genomics ... S-(dimethylarsenic)cysteine. Library files and coordinates for GMP and S -(dimethylarsenic)cys- teine were obtained from the HIC-UP ligand database [36] and generated at the prodrg server [37]....

Ngày tải lên: 15/02/2014, 01:20

... biochemical and biophysical properties of WhiB2, WhiB5, WhiB6 and WhiB7 of M. tuberculosis and also compare the prop- erties of all seven WhiB proteins. We show that, simi- lar to WhiB3 and WhiB4, ... refolding were  98% pure and were brownish (Figs S1 and S2). The presence of four conserved cysteines and the brownish appearance of purified WhiB1, WhiB2, WhiB5, WhiB6 and WhiB7 indicated the ... structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv Md. Suhail Alam, Saurabh K. Garg* and Pushpa Agrawal Institute of Microbial Technology, CSIR, Chandigarh,...

Ngày tải lên: 18/02/2014, 12:20

... coordinates and structure factors of the crystal structures described in this study have been submitted to the Protein Data Bank, and the structures have been assigned the accession codes 2v4n and 2v4o ... are represented by pink, cyan, blue and yellow, respectively. The tetra- meric interactions are between the subunits A and C, and B and D, by way of the b hairpins and the loop, which connects b2tob3 (boxed). Structure ... Science and Technology (DST) and the Depart- ment of Biotechnology (DBT) of the Government of India. MRN and HSS thank DST and DBT for finan- cial support. AP acknowledges the Council for Scientific and...

Ngày tải lên: 18/02/2014, 14:20

... 2,3 and 4, lower band). The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpress- ing IL-1b (Fig. 5, lanes 3 and 4, upper bands). ... (LSHM-CT-2006-036903, MTKD-CT-2006-042586 and COST Action BM0602) and by the Polish Ministry of Scientific Research and Information Technology (2 P05A01127, 63⁄ 6PR- UE ⁄ 2007 ⁄ 7 and 339 ⁄ 6PR-UE ⁄ 2007 ⁄ 7). ... intermediate levels in heart and placenta, and the lowest levels in spleen, kid- ney, liver and lung. The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig. 3A)....

Ngày tải lên: 18/02/2014, 14:20

... performed with rabbit preimmune and post-third inoculation sera against a M. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac- tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition ... addition of cysteine at the amino and carboxy termini and mixed with Freund’s Incomplete Adjuvant (Sigma)] on days 0, 20 and 40. Final bleeding was carried out on day 60 and sera were collected in accordance ... (Fig. 5A). Scat- chard and Hill analyses were then performed and dis- sociation constants (K d ) were calculated for Rv2560 HABP 11024 for U937 and A549 cell lines (K d of 380 and 800 nm, respectively)....

Ngày tải lên: 18/02/2014, 16:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... water molecule fix- ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E. A phosphate ion was found in the donor site ... The mutant enzymes, F133N and F133A, were expressed, purified and characterized. With 1 mm UMP and ATP as substrates, the activities of F133N and F133A were only 50 and 20% of that of the wild-type...

Ngày tải lên: 18/02/2014, 16:20

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... follows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). Reactions were carried out for 30 min at 65 °C and the products resolved by ... measured at 65 °C) were as follows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). (B) Dependence of Rep245 polymerase activity on...

Ngày tải lên: 18/02/2014, 18:20

... diversity in sequence, quaternary structure and functions such as binding and transport of oxygen and hydrogen sulfide, and activity of superoxide dismutase and mono-oxygenase [8]. Annelid noncirculating ... reversibly bind oxygen and other gaseous ligands, and are wide- spread in the three major kingdoms of life [1,2]. Despite the great diversity of their amino-acid sequences, the basic functional unit ... numbers AJ880690 and AJ880691 for cDNAs, Q53I65 and Q53I64 for amino-acid sequences) were aligned and compared with other globins in the mul- tiple sequence alignment (Fig. 1 and Table 1). The sequence...

Ngày tải lên: 19/02/2014, 02:20

... glucose and hemin chloride (13 lgặmL )1 ), and further incubated at 30 C for 24 h. Then, the cells were collected and suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed ... soyasapogenol B, and soyasapogenol E, oleanene sapogenols from soybean, structures of soyasaponin I, soyasaponin II, and soyasa- ponin III. Chem Pharm Bull 30, 2294–2297. 35 Ireland PA & Dziedzic ... aglycone structures are restricted to two, soyasapogenols A and B (Fig. 2). Soyasapoge- nol A has four hydroxyl groups at C-3, C-21, C-22, and C-24, whereas soyasapogenol B has three at C-3, C-22, and...

Ngày tải lên: 19/02/2014, 07:20

... disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, and the inter- monomeric bonds to consist of Cys274 and 286 [57]. At present, analysis of PDGF ⁄ VEGF ... PDGF-A and -B can form both homo- and heterodimers (PDGF-AA, -AB, -BB), PDGF-C and -D exist only as homodimers (PDGF-CC and -DD). The full-length PDGF-C and -D monomers are 54–55 kDa and 49–56 ... PDGF-c (exons 5 and 6) and PDGF-d (exons 6 and 7) encoding the cystine knot motifs resemble the corresponding exons in the PDGF-a and -b (exons 4 and 5) genes. Both in PDGF-a and -b, exon 6 encodes...

Ngày tải lên: 19/02/2014, 07:20

... Fish and molluscan metallothioneins A structural and functional comparison Laura Vergani 1 , Myriam Grattarola 1 , Cristina Borghi 2 , Francesco Dondero 3 and Aldo Viarengo 3,4 1 ... FEBS right-handed b domain binds three bivalent metal ions. The C-terminal a domain is left-handed and binds four bivalent ions. Zinc is preferentially located in the b do- main and cadmium in ... snail and oyster) have so far been elucidated [11–17]. As in mammals and fish [18], echinoderm MTs contain two globular domains binding four and three bivalent ions [19]. On the other hand, in...

Ngày tải lên: 19/02/2014, 07:20

... PQE- 30-AK, were washed, folded and purified as described in Materials and methods. Purified enzyme aliquots were maintained at 4 °C(m), 13 °C (j) and 26.5 °C(d) and AK activity was measured at ... in Asian and Mediterranean countries [15]. This paper describes the cloning and functional characterization of an AK gene from a genomic library of L. donovani as well as its expression and molecular ... characterization. Materials and methods Materials Plasmids pGEM-3Zf(+) and pQE30 were from Promega and QIAGEN, respectively. [ 32 P]dCTP[aP] (3000 Ciặ mmol )1 ) was from DuPont-NEN. Restriction enzymes and Taq DNA...

Ngày tải lên: 21/02/2014, 00:20

Ngày tải lên: 21/02/2014, 03:20