production isolation and purification of the exopolysaccharide eps

... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to other oxygenases On the contrary, the N-terminus of PHI is identical to that of MopN from A calcoaceticus...

Ngày tải lên: 21/02/2014, 00:20

... result in the increasing of the K values of curdione (1), curcumol (2) and germacrone (3), and the decreasing of the K values of curzerene (4), 1,8cineole (5) and b-elemene (6) The K values of all ... held well in the rotor and it came out with the effluent all the time during the whole process of separation Then, the revolution speed was increased to 1600 rpm and the flow rate of the mobile phase ... compounds The K values were determined by GC-MS analysis as follows: One drop (about mg) of the essential oil was added to the mixture of equal volume of the upper phase and the lower phase of the...

Ngày tải lên: 07/03/2014, 21:20

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... bold The position of the terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation of the GAGA motif only slightly altered the ZAS-N...

Ngày tải lên: 30/03/2014, 13:20

... purification, the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification are required for the accurate characterization of these ... 1961), and therefore we assume that FMN is lost during the purification process To achieve better stability of the enzyme, FMN was added to the protein solution immediately The requirement of non-heme ... defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase The 2,5-diketocamphane...

Ngày tải lên: 21/06/2014, 02:20

... validation of their structure and characterization of their functions Even after the identification, the next would be to understand how the molecular components of the cells are controlled, interact and ... third of the human genome consists of repetitive sequences Almost all of these have arisen by retroposition of an RNA intermediate followed by insertion of the resulting cDNA into the genome Of these, ... process of integration, the viral genome breaks at E1 and E2 regions, never at the E6 and E7 region The loss of 19 Literature Survey E2 results in the loss of E6 and E7 regulation This allows the...

Ngày tải lên: 16/09/2015, 17:13

... động cột Yếu tố góp phần khoanh vùng lan truyền khuếch tán theo chiều dọc cột, thiếu cân bằng, lấp đầy cột không đầy đủ Khuếch tán theo chiều dọc giảm thiểu cách sử dụng dòng chảy tốc độ cao ... như: µ = µ max đó: ks cs cs cs + k s µ max tốc độ tăng trưởng đạt tối đa bão hòa nồng độ chất Theo mối quan hệ tốc độ tăng trưởng thực tế nồng độ chất thấp thấp giá trị tối đa xác định thuộc ... chức quy mô Trộn, sục khí, làm mát kiểm sốt tốt thống quy mô mức 10L , tất thơng số vận chuyển trì theo cách quy mơ lớn Biến đổi quy mô tiếp tục phát sinh từ phản ứng tế bào với giá trị phân phối...

Ngày tải lên: 18/11/2017, 19:19

... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported ... correlations The determination of the 3D structure of D desulfuricans ATCC 27774 NrfA [17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes In addition, the first partial spectroscopic and redox characterization of the NrfH subunit is also...

Ngày tải lên: 21/02/2014, 00:20

... alignments and for CDRs and In these cases extensive loop modelling was undertaken and the final model selection based on the modeller objective score Construction of the 12F-11DCys mutant and 12F-11 ... background, and all of these proved to be identical sequences One of these, designated clone 12F-11, was chosen for further analysis The primary and deduced amino acid sequences of clone 12F-11 ... underlined, and the alanine linker and dual octapeptide FLAG tags are italicised The positions of the CDR1 and )3 regions are indicated in bold type (B) Alignment of protein 12F-11 with four other VNAR...

Ngày tải lên: 17/03/2014, 10:20

... assets of the total enterprise capital It is the profitability of equity Profitability of equity represented by the relationship between the profitability of enterprises with equity, capital of the ... Hanoi and a branch in Ho Chi Minh, with the system of organization and management comply with the ISO standards and the software development process closely, science, served the demand of indigenous ... the success or failure of the enterprise depends on the dedication and enthusiasm of the employees because they are thrust to the enterprise forward The business strategy of the next years, iThangLong...

Ngày tải lên: 22/03/2014, 11:20

... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of ... plasmid DNA containing the 25S rRNA genomic DNA sequence The probe was made by random priming and the incorporation of [a-32P]dCTP Hybridization of the probe to the membrane and subsequent washes ... Tsurugi K (1987) The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins J...

Ngày tải lên: 23/03/2014, 07:20

... methods they should be mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility To the fullest extent of the law, neither the Publisher ... with the mission of the enterprise The literal image of the company is in your hands 1 CASE STUDIES Introduction Digital media and broadband connectivity have influenced the evolution of video production ... montage of the history of the company and husband and wife team who built it from the ground up The video was a hit with the employee development department, and the VP of the division who oversaw...

Ngày tải lên: 30/05/2014, 23:13

... EA: Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 ... data and wrote the manuscript; CA performed research and analyzed data; ES and FB analyzed data; SC and GM were responsible for patient care and provided clinical information; AG, CM, SR, and ... analyzed data; SAP and PPP performed research, analyzed data and wrote the manuscript All authors read and approved the final manuscript VT and CA contributed equally to this work; SAP and PPP contributed...

Ngày tải lên: 10/08/2014, 23:21

... Institute of CAAS, Harbin, China 150001 Authors’ contributions GY and HZ carried out the molecular studies, and drafted the manuscript SC participated in the design of the study and conceived of the ... the bacmid The transposition assay and subsequent transfection steps were the same for all vectors White colonies contained the recombinant bacmid, and therefore were selected for isolation of ... cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and safety...

Ngày tải lên: 11/08/2014, 21:21

... LG5 and LG9 have been improved with the integration of the HQT and HCT genes The former increased the number of bridge markers on LG5, and reduced some large gaps (of 10 cM and cM) affecting the ... in the transesterification of caffeoyl-CoA and quinic acid, a reaction which occurs in the first route of CGA biosynthesis, but also at the end of the third pathway, following the action of HCT ... diagram of enzymes and major products in the synthesis of chlorogenic acid in plants A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in plants The product...

Ngày tải lên: 12/08/2014, 03:20

... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15% SDS-PAGE of cultures ... acid extension in C-terminal MALDI-ToF MS and phylogenetic analysis The MALDI-ToF mass spectrum of tryptic digest of the gel band of His-CP was shown in Fig.2a The achieved peptide masses were searched...

Ngày tải lên: 12/08/2014, 04:21

... cloning the PCR products in the Topo 2.1 vector, p24 was cloned downstream of RTB by ligating the XhoI site on the 3' extreme of RTB to the SalI site at the 5' of the p24 sequence (Figure 1B) The ... part of the panels Panel A, p24 construct The arrow indicates the expected band of about 34 kDa Panels B and C, RTB/p24 construct The arrows indicate the expected chimeric protein (RTB/p24) of ... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant...

Ngày tải lên: 12/08/2014, 04:21

... evaluate the effect of the formulation components and the spray-drying process on the PT immunogenicity of the HAC1 vaccine Based on the in vitro evaluation of the antigen RI content (process loss and ... adjust the solubility of the polymer and the drug and the boiling point that results in particles with the desired properties In the latter case, the advantage of water is associated with the non-toxicity ... advantages and challenges of the spray-drying method for the production of pure drug particles and drug-loaded polymeric particles and discusses the potential of this technique and the more advanced...

Ngày tải lên: 02/09/2015, 13:31

... This result in the successive generation of two active forms of the factor: aFXIIa and bFXIIa The cleavage of the Arg353-Val354 bond of FXII results in the formation of aFXIIa form of the enzyme, ... following either damage or activation of the endothelium This may occur due to the perforation of the vessel wall or activation of the endothelium (Geczy, 1994) Upon activation of endothelial cells ... vascular injury, several steps occur to staunch the flow of blood These steps are synergistic and simultaneous Vasoconstriction lessens the diameter of the vessel slowing the loss of blood Primary hemostasis...

Ngày tải lên: 14/09/2015, 13:36

... expression of 13 Chapter I Introduction oct4 is regulated by the changes of DNA methylation The statue of DNA methylation is related to the changes of the chromatin structure of the mouse oct4 gene and ... Introduction 1.5 The objective of this study Because of the essential roles of Oct4 and Stat3 in maintenance of stem cell pluripotency in mammals, and the unique features of the medaka, it seems ... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...

Ngày tải lên: 14/09/2015, 13:38

... to GnRH receptors on the surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone ... dC-tail of the cDNA and served as an extended template for RT The RT switched template from the mRNA to the SMARTII oligonucleotide and synthesize the complete cDNA copy of the original RNA The first-strand ... dependent largely on the reproductive stage of the fish and the species, and to a lesser extent, the gender In the study of gonadotropin response to GnRH during sexual ontogeny of the common carp,...

Ngày tải lên: 08/11/2015, 17:15