preparation of mudj and mudk donor p22 transducing lysates
Báo cáo khoa học: " Pharmacokinetics and dosage regimen of ceftriaxone in E. coli lipopolysaccharide induced fever in buffalo calves" pot
Ngày tải lên :
07/08/2014, 18:21
... administration of drug are given in Table Taking and h as convenient dosage intervals (τ) with minimum therapeutic concentration C (min) of 0.2, 0.4, 0.6, 0.8 and 1.0 µg/ml and using the values of β and of ... detect a minimum of 0.1 µg/ml of ceftriaxone The standard curve of ceftriaxone in calf plasma was linear between 0.25 and 1.25 µg/ml The repeatability of this method was excellent and error within ... tissue compartments The rapid distribution of ceftriaxone was further substantiated by high values of K /K (2.30 ± 0.48) and K /K (0.63 ± 0.14) The values of V of ceftriaxone in healthy animals [6]...
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Báo cáo khoa học: " Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus" pot
Ngày tải lên :
07/08/2014, 18:21
... snoisel lasrub gnirocS snoisel gnirocs rof detcelloc erew sasrub eht dna ,ytilatrom rof yliad derotinom erew snekcihc eht fo llA snoisel lasrub gnirocs rof desu erew dna slortnoc evitagen degnellahcnu ... saw tellep eht dna nim 01 rof 0053 ta degufirtnec erew sllec eht ,ylfeirB sisylana tolb nretseW yb demrifnoc saw nietorp 2PV fo noisserpxe ehT gnitsevrah erofeb h rof deunitnoc saw noitabucni ... ,noitaluclac gniwollof eht no desab detaluclac erew sretit ydobitna ehT sllec IS-12LB eht ni nietorp 2PV fo noisserpxe rof noitidnoc lamitpo eht dedivorp lCaN fo M 3.0 htiw dna h rof Co03 fo erutarepmet...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
Ngày tải lên :
12/08/2014, 04:21
... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... with immobilized asialofetuin Five mg of asialofetuin were diluted in 0.1 M NaHCO3 and 0.5 M NaCl buffer, pH 8.3 and the solution was employed for immobilization of asialofetuin in Cyanogen bromideactivated-sepharose ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was...
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Tài liệu Khảo sát sự ô nhiễm Coliforms, E.coli, S.aureus trong kem, sữa tươi, bánh ngọt tại cửa hàng bán lẻ trên quận 4 của Hà Nội docx
Ngày tải lên :
19/02/2014, 10:20
... Dougle.Foodborrme Bacterial pathogen Edited Food Researcls institute University of wiseensin Madissen.(1993) P.360363.American 13 M and J.Kaper.Escherichia E coli Gastrointestinal Infections (chapter 16)...
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Báo cáo khoa học: Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin pdf
Ngày tải lên :
29/03/2014, 09:20
... the action of kasugamycin Growth of CVR40D and that of its parental strain MG1655 were compared in the presence of kasugamycin (Fig 5A) Addition of kasugamycin reduced the growth rate of MG1655 ... conformation of 16S rRNA, causing a movement of helix 44 and a global conformational change in the 30S subunit This is visible as the movement of the head of the subunit towards the body and flipping of ... subunit [14], and stimulates the GTPase activity of IF2 [15] It also increases binding of mRNA to the initiation complex in the presence of IF2 [16] Together with IF2, it stimulates drop-off of peptidyl-tRNAs...
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a course in universal algebra - s. burris and h.p. sankappanavar
Ngày tải lên :
31/03/2014, 14:57
... to universal algebra, and would consist of Chapter I; Chapter II except for §4, §12, §13, and the last parts of §11, §14; Chapter IV §1–4; and Chapter V §1 and the part of §2 leading to the compactness ... of lattice theory The origin of the lattice concept can be traced back to Boole’s analysis of thought and Dedekind’s study of divisibility Schroeder and Pierce were also pioneers at the end of ... source material plus state of the art texts or papers relevant to that section, and at the end of the book one finds a brief survey of recent developments and several outstanding problems The material...
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Production and Cost in the U.S. Paper and Paperboard Industry pdf
Ngày tải lên :
01/04/2014, 00:20
... conditions, and none of the biased technology coefficients were significant 15 V.4 Substitution and Demand Elasticities Table 4(a) and 4(b) report the Allen-Uzawa and Morishima elasticities of substitution ... elasticity of demand, reflected in Table by a large range and a standard deviation which is at least an order of magnitude larger than that for labor and materials In the last year of the sample, ... subsectors Analyses of these product categories (e.g wrapping and packaging, newsprint, printing and writing papers, and household and sanitary papers) would improve our understanding of the competitive...
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Báo cáo lâm nghiệp: "Composition of psocid taxocenoses (Insecta: Psocoptera) in Fageti-Piceeta s. lat. and Piceeta s. lat. forests in the Western Carpathian Mts" pps
Ngày tải lên :
07/08/2014, 03:22
... AVZ together with fields of the 8th and 9th AVZ Only single habitats of the 7th AVZ occur in the field of the 4th and 5th AVZ From the view of hydricity (q-axis), habitats of the 7th AVZ are on ... number of species, N – number of specimens, HS – Shannon-Wiener index of diversity, ES – equatibility, HB – Brillouin index of diversity, EB – equatibility Nsp Biotope Table Values of indexes of ... Habitats of the 7th AVZ are situated “higher” than habitats of the 8th AVZ in the graph of x-q axis (Fig 1) and thus it is possible to state that biotopes of the 8th AVZ are more “moist” A field of...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
Ngày tải lên :
30/03/2014, 15:20
... start of the oraS gene and a BamHI site into the end of the oraE gene Genomic DNA was purified from C sticklandii by phenol/chloroform extraction methods [4] The coding regions for the S and E ... measuring the apparent Km value of D-ornithine at 25, 50, 100, 200 and 400 lM of the inhibitor For the measurement of the activity of the substrate analogues, an HPLC and NMR-based method was developed ... in various concentrations of PLP, and the binding of coenzyme was measured PLP was bound with an apparent Kd of 227 ± 41 nM (Fig 3) Fig UV–visible spectrum of wild-type and mutant D-ornithine aminomutase...
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báo cáo khoa học: "Engineering of the E. coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes" pdf
Ngày tải lên :
11/08/2014, 00:23
... University of Science and Technology, Khyber Pakhtunkhwa, Pakistan for funding Authors’ contributions NM and TD carried out design and performed study, data analysis and drafting of the manuscript ... Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins Proteomics 2003, 3:1418-1424 31 Laemmli U: Cleavage of structural proteins during the assembly of ... encapsulated into polymersomes and despite of using the Soret absorption band, the total amount of encapsulated enzyme could not be detected The kinetic data obtained in presence of the FhuA Δ1-159 Ext,...
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Canonical structures in potential theory - s s vinogradov, p d smith, e d vinogradova
Ngày tải lên :
17/03/2014, 14:41
... to dominate many areas of mathematics, physics, and engineering Scattering of electromagnetic or acoustic waves is of widespread interest, because of the enormous number of technological applications ... state, most of our understanding of how scattering takes place is obtained by detailed examination of such representative scatterers Their study provides an exact quantification of the effects of edges, ... theory of series equations and integral equations, and solves mixed boundary potential problems (mainly electrostatic ones) for structures with cavities and edges The theory and structure of the...
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Báo cáo khoa học: " Low numbers of intestinal Shiga toxin-producing E. coli correlate with a poor prognosis in sheep infected with bovine leukemia virus" pps
Ngày tải lên :
07/08/2014, 23:22
... groups and at a long-term disadvantage and making them more vulnerable to BLV, especially after cessation of STEC treatment at months and removal of protective effects of Stxs, present in and/ or ... is consistent with the lack of correlation between STEC scores and weight gain in groups and 2, as opposed to group 3, and with the clustering of cases of poor health and tumor in group 1, that ... challenge, and autopsied Gross pathology was noted, and tissue samples preserved in 4% buffered formaldehyde Sections of lymph nodes (retropharyngeal, prescapular, submandibular, and mesentheric)...
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Báo cáo khoa hoc:" Failure of E. coli bacteria to induce preterm delivery in the rat" ppsx
Ngày tải lên :
11/08/2014, 07:21
... and direct injection routes of administration of × 108 bacteria and p = 0.003 for comparison of the two routes of administration of × 108 bacteria) Finally, each of pregnant dams who received ... intraperitoneally) of pregnant Sprague Dawley rats on day 18 and again on day 19 of pregnancy produced white matter injury, myelination defects and apoptosis and increased expression of IL-1β, TNFα and IL-6 ... trans-cervical infusion of various volumes of sterile solution was feasible and rarely affected the course of pregnancy Trans-cervical intrauterine infusion of a pathogenic strain of killed E coli fails...
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Báo cáo khoa học: " Acute phase response in two consecutive experimentally induced E. coli intramammary infections in dairy cows" pptx
Ngày tải lên :
12/08/2014, 18:22
... Uden, The Netherlands) Milk and serum samples were initially diluted 1:500 and 1:1000 http://www.actavetscand.com/content/50/1/18 respectively, and assayed following the instructions of the manufacturer ... (hours) Figure Concentrations of SAA, LBP and Hp in serum and blood leukocyte counts in two consecutive E coli challenges Concentrations of SAA, LBP and Hp in serum and blood leukocyte counts in ... Green LE, Green MJ: Survey of the incidence and aetiology of mastitis in dairy farms in England and Wales Vet Rec 2007, 160:253-258 Peeler E, Fitzpatrick J, Green L: Study of clinical mastitis in...
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Báo cáo khoa học: "Transfer of immunoglobulins through the mammary endothelium and epithelium and in the local lymph node of cows during the initial response after intramammary challenge with E. coli endotoxin" ppt
Ngày tải lên :
12/08/2014, 18:22
... isotypes and bovine of Escherichia (BSA) in afferent and efferent lymph, milk and blood Concentrations of after intramammary infusion of 50 μgserum albumincoli endotoxin Figure Concentrations of immunoglobulin ... compartments, and the Ig traffic through endothelium and epithelium, and in the local lymph nodes of the bovine udder We believe this has not been described previously The cell traffic and content of cytokines ... al [16] A number of vital functions were checked and registered from the start of the anaesthesia until the end of the experiment to control the general condition and hydration of the animals:...
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Báo cáo y học: "Dramatic increase of third-generation cephalosporin-resistant E. coli in German intensive care units: secular trends in antibiotic drug use and bacterial resistance, 2001 to 2008" pot
Ngày tải lên :
13/08/2014, 20:22
... proportions of β-lactamase-sensitive penicillins, of penicillins with extended spectrum, of β-lactamase-resistant penicillins and of penicillins with β-lactamase inhibitor among the whole class of penicillins ... feedback of the data to the ICUs is a type of intervention) or promotion of hand hygiene could have influenced antibiotic use and antimicrobial resistance in individual ICUs The number of ICUs ... hospital setting and consumption often runs in parallel to the pattern seen for resistance The goal of the present study is to give an overview of changes in antibiotic consumption and resistance...
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Báo cáo y học: "DNA signatures for detecting genetic engineering in bacteria" ppt
Ngày tải lên :
14/08/2014, 08:20
... Figure of k-mers that are candidate signatures Percentage Percentage of k-mers that are candidate signatures The red line plots the percentage of candidate vector signatures as a function of k ... in the legend and are described by their k-mer size (30 and 60) and the signature set origin (large, small and MCS-only) The large and small sets are k-mer derived signature sets and MCS-only ... between A and B exists if the intersection between VA and VB is non-empty and the edge is labeled with the names of the shared vectors For example, in Figure there is an edge between k-mer set and...
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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
Ngày tải lên :
14/08/2014, 13:21
... chips of the SCS-BTA18-q group and two chips of the SCS-BTA18-Q group were removed, because of higher centered and larger spread boxes in NUSE (Normalized Unscaled Standard Error) plots and an ... [28] was chosen, and the maximum number of profiles was set to the default value of 50 considering a maximum unit change of between profiles Contrary to Analysis 3, in Analysis and Analysis the ... homeostasis Page of 17 Effect of inoculation with heat inactivated S aureus and E coli pathogens on gene expression in primary bovine mammary gland cells between and at time points 1, and 24 h of inoculation...
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