preference for air movement
Regulation and functional analysis of the tumor suppressor gene product, p53
Ngày tải lên :
15/09/2015, 17:10
... (940C for 35sec, 520C for 1min, 720C for 50sec) X 25 cycles, 720C for 10min Size of the PCR product: 500bp p21/Waf : 510C for 30min 30sec, 950C for 15min, (940C for 30sec, 540C for 30sec, 720C for ... 510C for 30min 30sec, 950C for 15min, (940C for 40sec, 520C for 1min, 720C for 1min 20sec) X 35 cycles, 720C for 10min Size of the PCR product: 750 kb 14-3-3σ : 510C for 30min 30sec, 950C for ... for 1min 30sec, (940C for 25 sec, 530C for 35sec, 720C for 40 sec) X 23 cycles, 720C for 8min, Hold at 40C Size of the PCR product: 500bp p53R2 : 940C for 1min 30sec, (940C for 25 sec, 540C for...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
Ngày tải lên :
11/08/2014, 08:21
... http://www.jibtherapies.com/content/8/1/9 for hour at room temperature with gentle agitation Blots were agitated for hour with HuMAbs at μg/ml diluted in 5% milk/TBS-T The blots were then washed four times (for each time ... surface was performed by two injections of 10 mM HCl for 30 seconds at a flow rate of 100 μl/min The on- (ka) and off-rate (kd) of HuMAbs were determined by observing the signal over time for triplicate ... performed to assess noise, and to normalize injection data All samples were randomly injected for two minutes using the KINJECT command and the dissociation phase of binding was observed for...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
Ngày tải lên :
09/09/2015, 08:16
... Shyue Wei – thank you for the antibodies and advice I am especially grateful for Angeline for being ever so patient with teaching me molecular biology and Conrad and Dominik for the initial generation ... another To Lam, for all the insightful intellectual discussions and for being a huge source of motivation To my Dad, for the bottles of celebratory champagne he got me, my mum for making sure ... towards the fusion peptide forming a hairpin-like structure This mechanism of folding back forces the target membrane and viral membrane to bend towards each other for its subsequent fusion The...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
Ngày tải lên :
14/09/2015, 13:38
... with shaking for 16-24 h The probe was visualized by specifically immunostaining for digoxigenin The slides were then washed three times for 30 in 50% formamide, × SSC at 65°C and once for 30 in ... important for proteinprotein interactions and for dimer-dimer interactions to form tetrameric STAT molecules Deletion of the amino terminus leads to STAT binding to single sites, tetramers are not formed ... activity is not required for fate specification but is essential for anterior migration and convergence and extension These results establish a role for Stat3 in the control of cell movements during...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... internal control for RT-PCR PCR conditions were as follows: one cycle of 94 °C for min, 32 cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s, followed by one cycle of 72 °C for PCR products ... from cDNA A negative control (no template) reaction was also performed for each primer pair tested A melting curve for each PCR was performed between 72 and 94 °C to ensure that only a single product ... homologue for OSBPL7 was found in chromosome 12, for PPP1R3F in chromosome and for stat6 in chromosome 23 Subsequently, the DNA surrounding these genes was retrieved ( 300 000 bp) for further...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... the metabolism of ROS, and some organic selenium compounds mimicking GPX have therefore been synthesized [24–28] For example, ebselen, a five-membered ring seleniumcontaining heterocyclic compound, ... in a dose-dependent manner The other compounds gave similar results to those for selenourea A CL inhibition curves for the four organic selenium compounds are shown in Fig The 50% inhibitory concentration ... before PMA treatment Dose-dependent O2–-scavenging effects of the compound were found by eliminating MCLA-dependent CL Arrows indicate the time at which MCLA or PMA was added O2– was recorded for...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
Ngày tải lên :
23/03/2014, 21:20
... and corrected for NADH oxidase activity by omitting phosphoenolpyruvate from the reaction mixture Phosphatase assay B Acid-washed microtitre plates were used for all kinetic studies For substrates ... dried under a stream of air for 7–8 h Acetone pellets were resuspended in 50 mM Na-acetate (pH 5.8) containing mM MgCl2 using a Polytron (3–5 s pulses) The solution was stirred for 60 min, clarified ... difluoride) membrane for 40 at 250 mA The membrane was washed in Milli-Q H2O, stained for 10 with 0.1% (w/v) Coomassie Blue R-250 in 40% (v/v) methanol and 1% (v/v) acetic acid, destained for 10 in 50%...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
Ngày tải lên :
24/03/2014, 04:21
... HotStarTaq DNA polymerase, amplification was performed as follows: five cycles of denaturation at 94 °C for 45 s, annealing at 46 °C for 45 s and extension at 72 °C for 120 s, followed by 35 amplification ... centrifugation at 3200 g for 20 at °C, and the cellular fraction was washed in NaCl/Pi buffer and processed for total RNA purification using a RNeasy Blood kit (Qiagen) Synthesis of cDNA was performed by oligo(dT) ... bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible human homologue in mammary epithelium RT-PCR was performed on the RNA isolated from the cellular...
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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
Ngày tải lên :
12/08/2014, 01:21
... in the attenuation of this virus in the natural host Unfortunately, these two mutations close to heparin interacting regions cannot account for the characteristics of the PanAsia strains isolated ... swine in Fujian province of China in 1999, perform plaque assay and estimate the pathogenicity in suckling mice, determine the complete genomic sequence for comparison with O/YUN/TAW/97 and 14 reference ... cytopathic effect (CPE) was adapted to BHK-21 cells, and formed clear large plaque However, the FMDV bovine-isolated O/Tibet/CHA/1/99 strain formed small plaque shaped a fringe of snowflakes (Fig...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
Ngày tải lên :
13/08/2014, 05:21
... densitometer (GS-800, Bio-Rad, California, USA) For immunocytochemistry, cells were plated on glasscover slips and cultured for 24 h Cells were fixed with 4% paraformaldehyde in PBS, permeabilized ... Bio-Rad, California, USA) Purified RNA (2 μg) was used as a substrate for single-stranded cDNA synthesis An aliquot (5 μl) of the cDNA of each sample was used for PCR with primers for IFNγ, TNF-α, ... DNA mixture was amplified for 30 cycles (each consisting of denaturation 45 sec at 95°C, annealing for 45 sec at 58°C, extension for at 72°C), received a final extension for 10 at 72°C and was stored...
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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx
Ngày tải lên :
13/08/2014, 13:20
... at 95°C for min, followed by 40 cycles consisting of denaturation at 95°C for minute, annealing at 60°C for minute, elongation at 72°C for min, and, finally, an elongation step at 72°C for 10 ... use For the detection of HepPar1, slides were deparaffinized, immersed in 10 mM Tris, mM EDTA buffer (pH 9), heated in a microwave oven for 10 minutes for antigen retrieval, cooled down for 10 ... culture, in the liver tissue antigen retrieval for CK7 was necessary and, therefore, a 40-minute proteinase-K (Dakocytomation) digestion at RT was performed before blocking of the endogenous peroxidase,...
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Báo cáo sinh học: " Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs" potx
Ngày tải lên :
14/08/2014, 19:22
... reported [16] HCV core Ag proved to be useful for performing HCV RNA measurement among dialysis patients in routine laboratories without the need for special equipment or training [17] E1 protein ... incubated at room temperature for one hour The membrane was washed for three times with PBS-T Substrate tablet (NBT/BCIP) was dissolved in 1XPBS and blot was incubated for 15-30 Generation of stable ... have better information of viral pathogenesis in order to develop new anti-HCV strategies To test novel drugs for its inhibitory action, an efficient culture system is required for the amplification...
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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
Ngày tải lên :
12/09/2015, 09:55
... 63 2.1.3 Cloning strategy 64 2.1.4 Transformation of E coli cells 64 2.1.4.1 Electroporation mediated transformation 64 2.1.4.2 Heat-shock induced transformation 65 2.1.5 Plasmid DNA preparation ... distributes uniformly throughout the whole cell cortex (Boyd et al., 1996; Etemad-Maghadam et al., 1995) PAR-1 is required for all cytoplasmic asymmetries but is not required for the initial ... mutants, both asters are round and the pulling force at each pole is low; in par-3 mutants both asters are flat and the pulling force at each pole is high The force imbalance between posterior pole and...
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Development and characterization of a SARS coronavirus replicon cell line
Ngày tải lên :
04/10/2015, 15:46
... After incubation at 94℃ for 30 seconds, the following cycling program was started: 35 cycles with each cycle consisting of 94℃ for 30 seconds, 55℃ for 30 seconds, and 68℃ for to minutes (~1 minute ... were heated to 72℃ for 10 minutes, cooled to 4℃ and then stored at -20℃ The gene-specific primers used were 09F and 11R for the amplification of B, 12F and 14R2 for C, NFX and 9R for N The GFP-BlaR ... trimer (Delmas et al., 1990), is important for binding to cellular receptor and for mediating the fusion of viral and host membranes and thus is critical for virus entry into host cells (Collins...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
Ngày tải lên :
05/10/2015, 22:31
... it I thank Pankaj for his help, especially during my pre-thesis defense and Toan for his cheerful spirit, for enlivening me at all times A note of thanks to all members of Lab for always being ... anterior-posterior orientation of hair follicles The acquisition of polarity by migrating cells in skin epithelia is also likely to be essential for directional cell movements that occur, for instance, during ... Atrophin1 3.2.1 Touch up PCR for Fat1 and Atrophin1 33 3.2.2 Double digestion, phenol-chloroform purification and ligation of Fat1 33 3.2.3 Digestion, Phenol Chloroform extraction and Ligation...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
Ngày tải lên :
05/09/2013, 09:08
... distilled water at a concentration of 0.5 mg/ml and heated at 100°C for The estimation of flocculating activity for kaolin clay was performed using the procedure described above Other analyses Total ... used for the flocculating test Muddy water was prepared as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for ... collected and used for the flocculating test Deflocculated sludge was prepared by letting 75 ml of activated sludge to be suspended in 225 ml of distilled water followed by sonication for The sludge...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
Ngày tải lên :
05/09/2013, 16:11
... K for h When the solution was cooled to the room temperature, the solution was immersed in deionized water for 48 h, and then the sulfonated copolymer washed with deionized water and dried for ... components, with binding energies at 285.8 eV for the CH2 species, and at 290.43 eV for the CF2 species [26, 28], the component peak at 286.44 eV is assigned for aliphatic C—H species of the polystyrene ... the anti-oxidation performance of PVDF-SPS composite membrane should be done The overall findings suggest that the PVDF-SPS membrane offers the potential for improving the performance of the DMFCs,...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
Ngày tải lên :
22/12/2013, 08:58
... Poisson software for (a) mirror magnetic field, and (b) flat magnetic field are shown in figure The calculation was also performed analytically (Montgomery 1966) using standard relations for calculating ... coils with iron jacket (electromagnet) for shielding the fringing magnetic field and to reduce the power consumption The use of jacket is to provide a return path for the magnetic field lines and thereby ... conductor having a square cross-section of mm × mm with hole diameter mm for water cooling was used The optimized design parameters for solenoid coil with iron jacket are presented in table The optimized...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
Ngày tải lên :
13/02/2014, 04:20
... substrate By varying the temperatures, we found the optimal conditions for preparing TiO2 with high performance TiO2 films were formed on the glass substrates at temperatures in the range of 350-450 ... in order to form SnO2 For the best results, impregnations were carried out by varying concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different ... concentrations appropriate for spraying The obtained aqueous solutions were then subjected to a spraying process with the help of a glass atomizer, operating with an air stream at 1.5 to atm The...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
Ngày tải lên :
18/02/2014, 11:20
... site SP, signal peptide C D not be used as efficiently for translation initiation, and therefore may not interfere with translation from AUG3 For GmPDIL-3b, a 59 kDa polypeptide was generated from ... important functional role for these regions PDI requires both cysteine residues present in the redox active site for oxidase activity, but the N-terminal cysteine is sufficient for isomerase function ... were used for detecting GmPDIL-3a and GmPDIL-3b, respectively Primers for quantification of actin mRNA were as described previously [18] Each value was normalized by dividing it by that for actin...
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