preclinical characterization of engineered nanoparticles intended for cancer therapeutics

... Werner Tjarks, Marina V Backer, and Joseph M Backer Chapter Preclinical Characterization of Engineered Nanoparticles Intended for Cancer Therapeutics 105 Anil K ... development of nanoplatforms for cancer therapeutics is a critical component to the successful use of nanovectors for anti-tumor therapy and tumor imaging, there are other necessary components for nanoplatforms ... the area of cancer nanotechnology and performs preclinical efficacy and toxicity testing of nanoscale devices and materials The goal of the NCL is to support the rapid acceleration of preclinical...

Ngày tải lên: 04/06/2014, 14:30

... purified hEGFR for h at 37°C, 54.7% to 59.5% of the radioactivity was bound The addition of 10 μg of unlabeled fragment, which reduced the percentage of bound radioactivity to an average of 3.5%, demonstrated ... Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda MD 20892, USA Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, Center for Cancer ... Therefore, a peptic digestion was performed with g of panitumumab As with the previous preparation, LMW species were detected by SE-HPLC which comprised approximately 34% of the digest mixture For...

Ngày tải lên: 21/06/2014, 02:20

... 45 3.3.1 Characterization of PLMA 45 3.3.2 Characterization of PLMA-PEG 46 3.3.3 Characterization of PLMA-SPIONs 48 3.3.4 Characterization of PLMA-PEG-SPIONs ... magnetization curves of (a) ferromagnetic nanoparticles and (b) superparamagnetic nanoparticles     Figure 2-3  In vivo behavior of nanoparticles in blood vessels The EPR effect of nanoparticles is ... 3.3.5 Uptake of PLMA-SPIONs by Macrophages 55 3.3.6 Uptake of PLMA-PEG-SPIONs by Macrophages 59 3.3.7 Cytotoxicity of Nanoparticles 61 3.3.8 Magnetic Properties of Nanoparticles...

Ngày tải lên: 09/09/2015, 10:14

... selectivity of aptamers can thus be oriented through the choice of pertinent counter-selection/selection targets, as shown by reports of aptamers capable of discriminating between isoforms of protein ... subject of intense, sometimes excessive speculations for biomedical applications, often before B Tavitian Fig Oligonucleotides and biological information Table Oligonucleotides are modulators of gene ... combines the use of three basic properties of oligonucleotides: The possibility of creating very large families of combinatorial molecules The number of different molecules in a library of oligonucleotides...

Ngày tải lên: 29/06/2014, 11:20

... diagnosis of cancer and reducing incidence of cancer in high-risk individuals It could also potentially serve to monitor the progression of cancer Also, with an image map of the expression level of ... Synthesis and characterization of gold nanoshells…………………… …266 9.3.2 Optimization of irradiation dose for PTT…………………………… ….267 9.3.3 Optimization of gold nanoshells concentration for PTT………………….269 ... imaginary part of the frequency-dependent complex refractive index of gold as a function of wavelength………………… 53 Figure 2.3 Extinction efficiency of spherical gold nanoparticles of varying radii...

Ngày tải lên: 11/09/2015, 09:02

... efficiency of MMC were calculated using the equations listed below Loading content (% ) = Weight of drug in the nanoparticles × 100 Weight of nanoparticles Loading efficiency (% ) = Weight of drug ... system In vitro cellular uptake of different kinds of NPs To visualize the effect of FA-mediated endocytosis of different kinds of modified NPs, the distribution of rhodamine B-labeled NPs on HeLa ... Y, Maeda H: A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs Cancer Res 1986, 46:6387 Duncan...

Ngày tải lên: 20/06/2014, 22:20

... development of antibodies after several days of therapy.[14] Four monomers (of a molecular mass of 34 kDa each) form rasburicase, that is currently classified as detoxifying agent for antineoplastic ... efficacy of rasburicase, the advantages of PEG-uricase may be: lack of antigenicity, absence of side effects and a longer duration of activity Int J Med Sci 2007, 4 Rasburicase pharmacokynetics Information ... Syndrome (TLS) Already before 2002, when Food and Drugs Administration (FDA) of US has approved the use of 85 urate oxidase for the management of paediatric patients at risk for TLS (Tumor Lysis...

Ngày tải lên: 31/10/2012, 14:59

... self-assembly of the ECM [10,11] Notably, one of the characteristics of malignant transformation is downregulation of GAGs biosynthesis, especially of the HS chains [10,11] Low levels of cell-surface ... gateway for invading metastatic cells [23,25] Although these studies provided a proof -of- concept for the prometastatic and proangiogenic capacity of heparanase, the clinical significance of the ... functions in cancer Semin Cancer Biol 12, 173–186 12 Fuster MM & Esko JD (2005) The sweet and sour of cancer: glycans as novel therapeutic targets Nat Rev Cancer 5, 526–542 New concepts for heparanase...

Ngày tải lên: 15/03/2014, 23:20

... up; it’s a fact of life for anyone who captures thousands of images or video clips Another problem is that hard drive performance can taper off well before its capacity is reached For these reasons, ... in editorial fashion only and for the benefit of such companies with no intention of infringement of the trademark No such use, or the use of any trade name, is intended to convey endorsement ... raw format option for photographers It maintains all the quality and nondestructive editing benefits of proprietary raw file formats; however, unlike proprietary formats that often change over...

Ngày tải lên: 30/05/2014, 23:16

... set of Fourier measurements was proposed in [108] By using the fact the Fourier transform of the gradients of an image are precisely equal to a diagonal transformation of the Fourier transform of ... supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work Duplication of this publication or parts thereof is permitted ... amount of data collected by these systems compared to the quantity of information that is ultimately of use Compressive sensing can help alleviate some of the challenges associated with performing...

Ngày tải lên: 05/06/2014, 12:05

... Background Prostate cancer is the most frequently diagnosed cancer and the second leading cause of cancer- related deaths for males in the USA One promising approach in prostate cancer diagnosis ... injection of 64Cu/NOTA-monomer, the radiotracer was still in the form of its parent in the blood suggesting that the increased uptake of the tracer may not be related to the uptake of metabolites for ... before its use Peptide Synthesis We recently reported the synthesis, the characterization and the biological activity of NOTABBN(6-14) peptide [31] The general procedure for the preparation of...

Ngày tải lên: 21/06/2014, 17:20

... study of more than 200 patients [27] Objective outcome measures are required to assess any form of treatment and to date there is a substantial lack of information on the biochemical nature of cartilage ... the integrity and contour of the articulating surface, the degree of metachromasia with safranin O staining, the extent of chondrocyte cluster formation, the presence of vascularisation or mineralisation ... were chosen for several reasons: Morphology is thought to influence mechanical functioning of the tissue and is often of most interest to observers A smooth surface is important for articulation...

Ngày tải lên: 09/08/2014, 01:21

... metastatic cancers are being considered for surgical treatment [1] [17] Sophisticated dosimetric techniques allow estimation of the tumor and liver radiation doses [18] The extent of resection of liver ... tumor response, the patient's improved performance status, and the absence of any evidence of extrahepatic disease progression, a decision for a second course of SIRT (without chemotherapy) was made ... accomplishment of tumor control and FLR recruitment might offer a better therapeutic profile compared with that of PVE Clinical indications, patient selection criteria, and dosimetry for this therapeutic...

Ngày tải lên: 09/08/2014, 04:21

... al.: Development of criteria for the classification and reporting of osteoarthritis Classification of osteoarthritis of the knee Diagnostic and Therapeutic Criteria Committee of the American Rheumatism ... 220 µM of dNTPs and unit of ExTaq DNA polymerase (Takara Bio Inc.) The thermal cycle was minute at 94°C, minute at 62°C for ADAMs 8, 9, 10, 12, 17, 20, 21, 28 and 30, 67°C for ADAM15, 64°C for VEGFR-1, ... for VEGFR-2 and neuropilin-1 and 65°C for β-actin, and minute at 72°C, followed by minutes at 72°C for the final extension The nucleotide sequences of the PCR primers and the expected sizes of...

Ngày tải lên: 09/08/2014, 07:20

... (D99) of not more than 2% for 90% of patients Abbreviations: As in table estimate of SD was larger than what was reported in other studies that provided this information There are a number of possible ... precise estimates of IFM Our estimates of intrafraction motion, and therefore of CTV to PTV margin requirements, are based on a single pair of orthogonal during-treatment EPIs for each fraction, ... tracking of transponder position for minutes, to provide information about intra-fraction motion “Average” CTV to PTV margins, calculated using the method of van Herk [17], to give 90% probability of...

Ngày tải lên: 09/08/2014, 09:20

... Mitchell JB, et al: Application of a Page of macromolecular contrast agent for detection of alterations of tumor vessel permeability induced by radiation Clin Cancer Res 2004, 10:7712-7720 37 ... extravascular-extracellular space of the tumor are also the most likely mechanisms responsible for the transient increase in TV Obviously, the timing of the evaluation of tumor response after the start of treatment ... issue For the present study, we chose to perform MRI including DWI very early at a median of days following HDRBT Thus, we were enabled to obtain first information on the treatment response before...

Ngày tải lên: 09/08/2014, 09:20

... treatment planning for lung cancer? Radiother Oncol 1998, 48:71-77 Hof H, Herfarth KK, Münter M, Essig M, Wannenmacher M, Debus J: The use of the multislice CT for the determination of respiratory ... total dose of 34 or 36 Gy in fractions was delivered for metastatic lung cancers with a maximum diameter of less than 1.5 cm Pencil beam convolution with Batho power law correction of the Eclipse ... Alto, California, USA) with 6-MV photons The planned dose was 44 Gy in fractions for tumors with a maximum diameter of less than 1.5 cm, 48 Gy in fractions for tumors with a maximum diameter of 1.5–3...

Ngày tải lên: 09/08/2014, 09:22

... Development of a European Organization for Research and Treatment of Cancer (EORTC) questionnaire module to be used in quality of life assessments in head and neck cancer patients EORTC Quality of Life ... after the Figure Oral cavity cancer Oral cavity cancer Patient with a pT2N0M0 carcinoma of the left lateral border of tongue treated with postoperative radiation therapy for close margin and perivascularperineural ... dose to the submandibular gland 20 Gy completion of RT Mean score for dry mouth was 28.1 on a scale of 0-100, and 26.5 for sticky saliva; mean score for dysphagia was 4.6 on the same scale (Table...

Ngày tải lên: 09/08/2014, 10:20

... Within each tissue compartment, transfer of cells between the phases is determined by the rate of adhesion of cells from the fluid Page of (page number not for citation purposes) Radiation Oncology ... linear function of tumor cell number (first-order growth kinetics), i.e., the product of growth rate constant, and cell number for that compartment and phase For the purposes of this model, we ... course of treatment for the model allowing for flow (Qf = 25 ml/hr) and not allowing for flow (Qf = 0) are shown Cure is achieved in both settings This fits clinical experience; 54 Gy of radiation...

Ngày tải lên: 09/08/2014, 10:21