intended for cancer stem cell research

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic Stem Cell Research team derived stem cells from primordial ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 18 Guidelines for Human Embryonic Stem Cell Research THE NEED FOR GUIDELINES Since 1998, the volume of research...

Ngày tải lên: 06/03/2014, 15:20

... predict behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio­ temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche for cell survival and ... [3] For cell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression, and insights into how mechanical forces applied to individual cells ... translational stem cell biology can be addressed using microfluidics tools? Here again, microfluidics techniques afford the ability to define the microenvironment surrounding stem cells The disease...

Ngày tải lên: 06/08/2014, 19:21

... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool ... of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, cell- autonomous properties, ... Sánchez Alvarado A: Molecular analysis of stem cells and their descendents during cell turnover and regeneration in the planarian Schmidtea mediterranea Cell Stem Cell 2008, 3:327-339 Pfister D, De...

Ngày tải lên: 06/08/2014, 19:21

... Abbreviations NSCLC: non-small cell lung carcinoma; LCNEC: large cell neuroendocrine carcinoma; LCC: large cell carcinoma; SCLC: small cell lung carcinoma; CSC: cancer stem cell; LC: liquid chromatography; ... G-test; the exact p-values for LCNEC vs SCLC were 3.40 × 10-4 for AL1A1, 5.53 × 10-10 for AK1C1, 2.27 × 10-10 for AK1C3, and 0.012 for CD44 The G-test analyses of three cancer group pairs (LCNEC ... tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially in AC cell...

Ngày tải lên: 10/08/2014, 09:22

... and characterize the cancer stem cell in order to eliminate it It is an exhilarating time for stem- cell research The work presented at the meeting suggested that stem- cell research will lead to ... this approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing ... transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stem cell in leukemia If the results hold true for other tumors, the new challenge of cancer...

Ngày tải lên: 14/08/2014, 14:21

... lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell is a mutated stem cell that highly express stemness factors such ... distinguish precancerous stem cells from primary cancer stem cells First is the location Precancerous stem cells mainly exist in precancerous lesions, but primary cancer stem cells exist in primary cancer ... Relationships among cancer stem cell subsets Up to now, precancerous stem cells, primary cancer stem cells and migrating cancer stem cells have been proven to exist in the progression of cancer [45,52,61,62],...

Ngày tải lên: 18/06/2014, 19:20

... lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell is a mutated stem cell that highly express stemness factors such ... distinguish precancerous stem cells from primary cancer stem cells First is the location Precancerous stem cells mainly exist in precancerous lesions, but primary cancer stem cells exist in primary cancer ... Relationships among cancer stem cell subsets Up to now, precancerous stem cells, primary cancer stem cells and migrating cancer stem cells have been proven to exist in the progression of cancer [45,52,61,62],...

Ngày tải lên: 20/06/2014, 03:20

... subpopulation of cells called cancer stem cells (CSCs)” that are responsible for forming the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells ... Osteosarcoma Cancer Stem Cells CSCs have been proposed to arise either from the transformation of normal stem cells to cancerous stem cells or from the dedifferentiation and transformation of ... mesenchymal stem cells Stem Cells 2007, 25:371-379 Kuhn NZ, Tuan RS: Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol...

Ngày tải lên: 20/06/2014, 04:20

... subpopulation of cells called cancer stem cells (CSCs)” that are responsible for forming the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells ... Osteosarcoma Cancer Stem Cells CSCs have been proposed to arise either from the transformation of normal stem cells to cancerous stem cells or from the dedifferentiation and transformation of ... mesenchymal stem cells Stem Cells 2007, 25:371-379 Kuhn NZ, Tuan RS: Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol...

Ngày tải lên: 20/06/2014, 07:20

... overexpressed ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... population of stem/ progenitor cells in the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells ... HER2-overexpression in breast cancer cells enriched the cancer stem cell population, evidenced by an increased number of tumor sphere formation and cancer stem cell marker aldehyde dehydrogenase...

Ngày tải lên: 20/06/2014, 07:20

... niche enforces hematopoietic stem cell quiescence and is necessary to preserve self-renewal in vivo Cell Stem Cell Mar 6;2(3):274-83 Fuchs, E.(2008) Skin stem cells: rising to the surface J Cell ... a niche for hematopoietic stem cells Dev Cell Mar;8(3):365-75 Gering, M & Patient R.(2005) Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos Dev Cell Mar;8(3):389-400 ... of HSCs is not only critical for protecting the stem cell compartment and sustaining stem cell pools over long periods, but it is also critical for protecting stem cells by minimizing their accumulation...

Ngày tải lên: 27/06/2014, 13:20

... intestinal stem cell marker LgR5 might also be suited to identify cancer stem cells, these might be the actively cycling Barrett (cancer) stem cells Our findings are in line with current cancer models ... theory If LgR5 marks stem cells, there are many of LgR5 negative non -stem cells, which are nevertheless cycling Therefore a combination of clonal selection and cancer stem cell model, as previously ... not all, malignancies are driven by a cancer stem cell compartment [8] The existence of cancer stem cells would explain why only a small minority of cancer cells is capable of extensive proliferation...

Ngày tải lên: 10/08/2014, 10:21

... a novel marker for human hematopoietic stem and progenitor cells Blood 1997, 90:5002-5012 22 Wu Y, Wu PY: CD133 as a marker for cancer stem cells: progresses and concerns Stem Cells Dev 2009, ... G, Medema JP: Cancer stem cells - old concepts, new insights Cell Death Differ 2008, 15:947-958 Bomken S, Fiser K, Heidenreich O, Vormoor J: Understanding the cancer stem cell Br J Cancer 103:439-445 ... cancer stem cell; bFGF: basic fibroblast growth factor; FGFR: fibroblast growth factor receptor; HSC: hematopoietic stem cell; MSC: mesenchymal stem cell; ALDH: aldehyde dehydrogenase Stratford...

Ngày tải lên: 13/08/2014, 15:21

... particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses ... encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage repair(Hwang ... mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and is associated with increased cell adhesion,...

Ngày tải lên: 09/09/2015, 08:12

... Archival Formalin-Fixed Paraffin-Embedded Tissues 2.1.2 Samples for Studying Prognostic and Predictive Significance of Cancer Stem- Cell Markers 2.1.3 Samples for Studying the Implications of Cancer Stem- Cell ... monoclonal expansions of transformed cells - but rather as complex tissues where abnormal growth is driven by a minority of cancer stem cells These cancer stem cells possess tumour-related features ... the cancer stem- cell concept, tumours are not merely simple monoclonal expansions of transformed cells, but complex tissues where 12 abnormal growth is driven by a minority of cancer stem cells...

Ngày tải lên: 03/10/2015, 20:57

... samples for both the data sets with reasonably low FPR and high TPR For the melanoma data set, we obtained FPR of 1.33% for S2 and 16.8% for S3, based on the 10 ions selected by our model For the ... (Millipore) were conditioned on a robotic liquid handling system (FluidX XPS-96 for the cord blood plasma samples or Proteome Systems Xcise for the melanoma serum) using cycles (aspirate and dispense) ... labelled the samples into two groups - Low (< 30 CD45 sidescatter low/CD34+ stem cells/μL blood) and High (~100 cells/μL) stem content This collection of plasma produced 158 samples, each associated...

Ngày tải lên: 13/08/2014, 13:21

... Stem cells- unique candidates for therapeutic platform 1.2.1 What are stem cells 1.2.1.1 Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells ... Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells 23 1.2.2 Clinical relevance of stem cell vectors for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic ... sources of stem cells for gene cancer therapy must be established Therefore, this study aimed to test feasibility of cancer treatment by applying pluripotent stem cells including embryonic stem cells...

Ngày tải lên: 09/09/2015, 10:08

... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral ... positive in both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The...

Ngày tải lên: 07/03/2014, 00:20

... neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant for four ... b-actin (forward: 5′-CTA CAA TGA GCT GCG TGT GG-3′, reverse:5′-CAG TCA GGA TCT TCA TGA GG-3′) The thermo-profile was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for ... bone marrow stem cells in ischemic brain Acta Neurochir Suppl 2006, 99:123-124 Li J, Sun CR, Zhang H, Tsang KS, Li JH, Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells results...

Ngày tải lên: 18/06/2014, 16:20

... utilities for metastatic breast cancer Br J Cancer 2006, 95(6):683-90 Cancer Research UK [http://www.cancerresearchuk.org] Cancer Help [http://www.cancerhelp.org.uk] Association for International Cancer ... European Organization for Research and Treatment of Cancer Quality-ofLife Questionnaire -Cancer- 30 items; EORTC QLQ-LC-17: European Organization for Research and Treatment of Cancer Quality-of-Life ... small cell and non-small cell lung cancer University of Iowa Virtual Hospital; 1999 Hopwood P, Stephens RJ: Symptoms at presentation for treatment in patients with lung cancer: Implications for...

Ngày tải lên: 18/06/2014, 19:20