pore pressure induced by fluid injection

... sinusoidal fluid pressure was applied to the marrow cavity of the left ulna through an external fluid oscillatory loading unit The loading unit was controlled to generate changes in the fluid pressure ... that bone fluid flow induced by ImP can regulate the nutrient arterial adaptation Thus, the objective of this study is to evaluate nutrient vessel remodeling under dynamic stimulation by evaluating ... normal blood flow into the marrow cavity Augmentation of femoral marrow pressure and interstitial fluid flow, induced by femoral vein ligation, could significantly influence bone quantity under...

Ngày tải lên: 20/06/2014, 04:20

... when the denaturation is induced either by chemical denaturants or by hydrostatic pressure? To answer these questions the effect of sucrose and glycerol on the pressure- induced unfolding of the ... the 33-kDa protein induced by GdmCl in the presence of different sucrose concentrations Other conditions as in Fig Fig The unfolding of the 33-kDa protein induced by hydrostatic pressure in the presence ... trypsin induced by high hydrostatic pressure Biochem Biophys Res Commun 239, 150–154 Ruan, K., Lange, R., Meersman, F., Heremans, K & Balny, C (1999) Fluorescence and FTIR study of the pressure- induced...

Ngày tải lên: 31/03/2014, 01:20

Ngày tải lên: 07/08/2014, 20:23

... followed by injections of nutritive mixture solution (NMS; n = 7) In the ACLT+NS group, joints had undergone ATLC surgery followed by normal saline (NS) injections (n = 11) The intra-articular injection ... guidelines, and were approved by each laboratory's Institutional Animal Care and Use Committee ACLT surgery for induction of osteoarthritis Experimental OA in rabbits was induced by ACLT surgery in both ... played by each of these elementary nutrients, we investigated the therapeutic effects of intra-articular injection of NMS on osteoarthritic knee joints, as compared with the effect of injection...

Ngày tải lên: 09/08/2014, 10:20

... Restoration of disc height loss by recombinant human osteogenic protein-1 injection into intervertebral discs undergoing degeneration induced by an intradiscal injection of chondroitinase ABC ... dose and the induced concentration of BMP-2 were quite different The direct injection of 0.1 mg of BMP-2 was supraphysiological compared with the endogenous level of BMP-2 induced by simvastatin ... needle puncture, without gel injection) ; the stabbed group (with needle puncture, without gel injection) ; the gel-alone treatment group (with needle puncture, gel only injection) ; and the simvastatin...

Ngày tải lên: 12/08/2014, 11:22

... variations measured by thermodilution during VE and those induced by PLR These results were also positively correlated with respiratory variations in pulse pressure simultaneously measured by an arterial ... groups: responders and non-responders to fluid loading A patient was classified as a responder when an increase of more than 15% in ABF was induced by fluid loading Results Study population Twenty-three ... fluid responsiveness Considering all patients, the increase in ABF induced by PLR was correlated positively with that induced by VE 500 ml (r2 = Page of (page number not for citation purposes)...

Ngày tải lên: 13/08/2014, 03:20

... the alveolar epithelium caused by unstable alveoli recruiting and derecruiting [10,11]; and biotrauma, or inflammation secondary to the mechanical injury induced by volutrauma and atelectrama ... cmH2O pressure and held at this pressure for 60 s to normalize lung volume history The lungs were than allowed to deflate to atmospheric Page of 13 (page number not for citation purposes) pressure ... the end of the experiment, and in BAL fluid obtained at necropsy Specifically, elastase activity was determined by incubating either 100 μl serum or BAL fluid and 400 μl of 1.25 mmol/l methoxy...

Ngày tải lên: 13/08/2014, 03:20

... anesthetized rats as shown by previous experiments24 Our results showed that emulsified isoflurane preconditioning could reduce lung injury induced by hepatic I/R, as represented by decreased NF-κB activity, ... the lung Lung injury induced by hepatic I/R is thought of as a result of liver-derived TNF-α In fact, blockade of TNF-α by antibody neutralization greatly reduced hepatic I/R induced lung inflammatory ... isoflurane preconditioning group (Grade 2, Fig 1D), suggesting that lung injury induced by hepatic I/R was attenuated by emulsified isoflurane preconditioning 357 Pulmonary edema after hepatic I/R...

Ngày tải lên: 25/10/2012, 11:00

... This study was approved by the animal experiments committee of Mukogawa Women’s University Drugs Navelbine® Injection (VNR) and Adriacin® Injection (DXR) were kindly provided by Kyowa Hakko Kirin ... reactions, phlebitis induced by intravenous infusion of antineoplastic agents reduces the completion of chemotherapy The causative factors of phlebitis include the pH and osmotic pressure of the solution, ... respectively Experimental procedure The effects of rapid infusion and dilution of the injection solution on phlebitis caused by VNR was evaluated in a rabbit model, as reported elsewhere [4] Briefly, test...

Ngày tải lên: 26/10/2012, 09:57

... GDP-bound forms can therefore be limited by the intrinsic rate of GTP hydrolysis, by the action of a GAP, or by interaction with a cognate target GTPases governed by these factors are often referred ... assay is based on the protocol described by Webb and Hunter [33] whereby, in the presence of Pi, 2-amino-6-mercapto-7-methylpurine riboside is converted by purine nucleoside phosphorylase to ribose-1-phosphate ... Tris–HCl (pH 7.6) and mm dithithreitol, and broken by two passages through a French pressure cell at 8000 psi Debris and unbroken cells were removed by and centrifugation and the supernatant, which...

Ngày tải lên: 16/02/2014, 09:20

... agonist -induced apoptosis [26,27] In the present study, we investigated PPARc agonist -induced adipocyte apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced ... (GW) (10 lM) Apoptosis was measured by AO ⁄ EB staining (E) Pioglitazoneinduced 3T3-L1 adipocyte apoptosis measured by Hoechst 33258 staining (hoechst) (F) PGJ2 -induced 3T3-L1 adipocyte apoptosis ... Xiao et al Apoptosis induced by TZDs in 3T3-L1 adipocyte A B C D F E G Fig Akt-1 reduction in troglitazone (Tro) -induced adipocyte apoptosis (A) Akt-1 and pAkt-1 in troglitazone -induced 3T3-L1 adipocytes...

Ngày tải lên: 16/02/2014, 09:20

... vector (Novagen) A clone confirmed by DNA sequencing was grown in ampicillin-supplemented medium until A600 of 0.3–0.4 was reached, and protein expression was induced by adding mm isopropyl thio-b-d-galactoside ... defence against the oxidative stress by reducing reactive oxygen species [30] The present study aimed to establish the function of the Alr2505 protein encoded by the last gene of this operon, which ... experimental approaches tested, solubilization by urea was found to be the most efficient method of purification, and this method was used to obtain the enzyme followed by removal of urea and refolding Pure...

Ngày tải lên: 18/02/2014, 04:20

... DE-81 filters by shaking for 30 in 0.2 m KCl ⁄ 0.1 m HCl, after which the radioactivity was determined by scintillation counting Analysis of kinetic data Kinetic data were fitted by non-linear ... high-efficiency kinetics with low Km values is exclusively displayed by the tetramer forms, and lowefficiency kinetics with high Km values is displayed by the dimer forms These observations strengthen the previous ... on Sephadex G-25, separated from other deoxynucleoside kinases by ion-exchange chromatography on a DEAE column, and further purified by affinity chromatography on a 3¢-dTMP Sepharose column dThd...

Ngày tải lên: 18/02/2014, 12:20

... apoptosis induced by EGFR tyrosine kinase inhibitors Here, we demonstrate that JNK-phosphatase MKP-1 expression is controlled by a signal downstream of EGFR and that if this signal is abolished by an ... controlled by both protein kinases and protein phosphatases [22–24] Various types of stimuli activate JNK through phosphorylation by the dual-specificity kinase MKK4 or MKK7 [18,25] By contrast, ... of stress -induced apoptosis [29,30] To examine whether JNK might be activated by AG1478, we treated PC-9 cells with AG1478 for various periods (Fig 2A) Activation of JNK was measured by performing...

Ngày tải lên: 18/02/2014, 13:20

... was induced by httEx1-25Q-EGFP expression, and only a slight decrease (about 10%) was induced by httEx172Q-EGFP and httEx1-103Q-EGFP expression (Fig 7A) No significant effects were induced by either ... cytotoxicity was induced by EGFP expression The mitochondrial depolarization mediated by httEx1-103Q-EGFP expression was more drastic and was roughly similar to that induced by a 15 incubation ... stress induced by lactacystin suggests that the rather weak alterations in proteasome activities induced by httEx1-polyQ expression are not responsible for the oxidative stress generated by these...

Ngày tải lên: 19/02/2014, 06:20

... TMK-1 cells from apoptosis induced by the DNAdamaging agent ADR Pretreatment of the cells with 10 or 100 ngÆmL)1 EGF for 48 h markedly suppressed the cell death induced by 10 or 20 lm ADR, whereas ... ngÆmL)1 EGF pretreatment markedly suppressed the cell death induced by 10 lm, ADR but not that by 20 lm ADR (Fig 1A) To evaluate whether this ADRinduced cell death resulted from apoptosis, we looked ... prevents cell death induced by several chemotherapeutic agents including ADR and paclitaxel in human cancer cells [15,16] We showed that EGF protected TMK-1 cells from apoptosis induced by ADR (Fig 1)...

Ngày tải lên: 19/02/2014, 06:20

... phalloidin After injection, cells were incubated with 50 lm BAPTA AM for h at 37 °C (a–d) Cells were stained with mouse IgG antibody to identify injected cells, which are indicated by arrows in b ... incorporated in the microtubule wall BAPTA is thereby similar to most known microtubule depolymerizing drugs [22] In the same cells, BAPTA AM induced an extensive disruption of the actin cytoskeleton, ... stress fibres showing that BAPTA action could overcome the stimulation of actin polymerization induced by nocodazole (Fig 2A, g,h) In Rat2 cells treated with the actin depolymerizing drug cytochalasin...

Ngày tải lên: 19/02/2014, 16:20

... synthesis induced by VEGF165 and VEGF121 PF-4 does not affect VEGF121 -induced KDR phosphorylation We analysed the effects of PF-4 on the signalling pathways induced by VEGF165 and VEGF121 by investigating ... lation of PLCc induced by VEGF121 was unaffected by 10 lgÆmL)1 PF-4 (Fig 3A,B) These results were confirmed by kinetic studies of ERK activation The ERK phosphorylation induced by VEGF165 and ... compared the mechanisms by which PF-4 inhibits the DNA synthesis induced by VEGF165 and VEGF121 The DNA synthesis induced by VEGF165 and VEGF121 was strongly inhibited by PF-4 (10 lgÆmL)1) in...

Ngày tải lên: 19/02/2014, 16:20

... elucidated Therefore, we decided to study the changes in the apo-CRP structure induced by binding of cAMP and DNA by measuring uorescence resonance energy transfer (FRET) and uorescence anisotropy ... the uorescence intensity, increasing it by % 3.4% A pronounced increase in the Trp85 uorescence intensity was ể FEBS 2003 Conformational changes induced by cAMP and DNA binding to CRP (Eur J Biochem ... nonspecic DNA-binding state to the state characterized by high afnity and sequence specicity ể FEBS 2003 Conformational changes induced by cAMP and DNA binding to CRP (Eur J Biochem 270) 1419...

Ngày tải lên: 20/02/2014, 23:20

... effect of TS on LPS/IFN -induced NO production is caused by enhancement of iNOS protein induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN -induced NO production We ... IFN [28] NO production was not induced by 10 lM TS alone in the absence of LPS/IFN However, treatment with TS 24 h before the addition of LPS/IFN enhanced LPS/IFN -induced NO production about twofold ... on LPS/IFN -induced NO production in VSMC Therefore, we used a concentration of 10 lM TS in this study, except in some cases Furthermore, TS enhanced the amount of iNOS protein induced by LPS/IFN...

Ngày tải lên: 22/02/2014, 04:20