plasmodesmata in intercellular trafficking of proteins and nucleic acids

... Role of nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect ... Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking proteins Functions as scaffolds for signaling intermediates that stimulate actin elongation; binding partners ... proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates actin-binding in several proteins, such as villin and dematin [59] Both ABLIM-2 and -3 show high homology...

Ngày tải lên: 07/03/2014, 01:20

... primer binding sites) of the DPOL gene [2] was carried out in a nested format with the degenerate and deoxyinosine-containing (deg/dI) primers DFA, ILK and KG1 in the first PCR round and TGV and ... dashed lines The solid line represents the final kbp contiguous sequences of CgueCMV-1.1 and CgueCMV-1.2 (B) Pairwise alignments of the UL56 and gB proteins and the partial DPOL proteins of CgueCMV-1.1 ... contains mismatches within the LNA binding region Two of the mismatches were LNA-substituted (Figure 2) In the presence or absence of the LNA, a similar amount of amplimer was obtained in real-time...

Ngày tải lên: 18/06/2014, 18:20

... clude binding sites (i.e specific sequences) for nucleic acid-binding proteins and should also encode corresponding nucleic acid-binding proteins The use of known protein transcription factors and ... speed of synthesis and folding of the nascent protein chains and also to regulate protein vector binding to the nucleic acid molecules as well as protein-protein interactions (in protein complex ... exemplified by the following pairs of interactors: (i) biotin as nucleic acid label and avidin, streptavidin, related proteins or derivatives which bind biotin as part of a protein vector which is...

Ngày tải lên: 11/08/2014, 00:21

... length of 10, on average 85% of proteins contained a predicted IUR Twenty to 21% of mammalian proteins and 13% of chicken proteins contained some kind of tandem AAR and 12% of mammalian proteins and ... of proteins that might have a number of functions, including binding to other proteins and small molecules and providing flexibility in multidomain proteins In an analysis of repeat content of ... mammalian proteins and 9% of chicken proteins contained some kind of C4 repeat; 4.6% of IURs contained a tandem AAR and 0.5% a C4 AAR The proportion of proteins containing an IUR reported here...

Ngày tải lên: 14/08/2014, 21:20

... (Lys239)-intein 14 2.4 Expression of intein-fused proteins 14 2.5 Affinity purification & C-terminal biotinylation of recombinant proteins 15 2.6 SPR analysis 16 2.7 In vivo protein biotinylation in E ... BioEvaluation software installed on the BIAcore X 2.7 In vivo protein biotinylation in E coli For in vivo biotinylation of proteins in E coli., pMYB5 and pTYB-1 constructs containing two yeast proteins ... product53-57; and (4) the newly discovered alanine inteins, which contain a naturally occurring N-terminal alanine residue58 Figure Mechanism of protein splicing In the initial step of protein splicing,...

Ngày tải lên: 08/11/2015, 16:30

... incidence of preeclampsia based on the timing of delivery In our study, we intend to establish if any such association exists in the distinct climate of the tropical coastal city of Mumbai, India ... had no influence on the incidence of preeclampsia In contrast, lower temperature, higher rainfall and humidity and lower barometric pressure were related to the triggering of seizures in patients ... factors in hypertensive disorders, vaginal bleeding and premature rupture of membranes during pregnancy Gynecol Obstet Invest 1991, 32(2):88-90 Makhseed M, Musini VM, Ahmed MA, Monem RA: Influence of...

Ngày tải lên: 13/02/2014, 06:20

... (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the presence of 100 lM MPP+ and 50 lM dopamine (H) and in the presence of 200 lM MPP+ and 50 lM dopamine (I) Discussion MPTP infusion ... understanding of the role of various interacting components The formation of dopamine–quinone adducts (because of auto-oxidation of the neurotransmitter), especially dopaminochrome, with a-synuclein, ... presence of 100 lM neurotoxin ( , dotted line), in the presence of 200 lM neurotoxin ( , dotted line) and in the presence of neurotoxin and 50 lM dopamine (h, dashed line) • 1692 FEBS Journal 278...

Ngày tải lên: 14/02/2014, 19:20

... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... far, interactions with AP-2 have been described for many proteins For example, CBP ⁄ p300-interacting transactivator with ED-rich tail interacts with and coactivates all isoforms of AP-2, and ... temporary shut-off mechanism during unfavorable conditions [35,36] Little is known about the interaction of AP-2 and its binding partners in cartilage However, at least CBP ⁄ p300-interacting transactivator...

Ngày tải lên: 16/02/2014, 09:20

... developing and maintaining a credible integrated climate observing system.” The “Post-2002” report (NRC, 1999b) determined that “ensuring continuity of operational data, evaluating the readiness of ... contracts for each of the candidate sensors There have been final selections of the winning contractors in 1999 and 2000 The Ozone Mapping and Profiling Suite (OMPS) contractor was selected in early 1999 ... Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering communities The Council is administered jointly...

Ngày tải lên: 17/02/2014, 07:20

... establishing and promoting adherence to high-quality professional standards, furthering the international convergence of such standards and speaking out on public interest issues where the profession’s ... Discussions Understanding Business Risk Understanding Fraud Risk Significant Risks Understanding Internal Control Assessing Internal Control Design and Implementation Assessing the Risks of Material ... process and so they share the task of supervising the staff Jawad Kassab (a cousin of Suraj) is in charge of the finance function and John Rabeer is in charge of information technology (IT) 16 INTRODUCTION...

Ngày tải lên: 18/02/2014, 05:20

... subheading and then select the Proteomics link) One of the proteins of interest to the Cell Migration Consortium is paxillin, a scaffolding protein involved in focal adhesions [9] Shown in Fig ... contains 21 proteins involved in mRNA binding The 50S, or L, subunit contains 34 proteins, binds to tRNA, and mediates peptidyl transfer Shown in Fig 4A is the base peak chromatogram from a 90 min, ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD...

Ngày tải lên: 18/02/2014, 16:20

... International Journal of Finance, The Journal of Banking and Finance, The Journal of Finance, The Journal of Financial Economics, The Journal of Financial & Quantitative Analysis, The Journal of Industrial ... ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE AND ACCOUNTING Essays in Microstructure in Honor of David K Whitcomb Volume This page intentionally left blank ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE ... methods and directions from which to examine pre-existing theories and puzzles in finance, in both the investments and corporate finance areas It has seemingly created the most innovative and popular...

Ngày tải lên: 19/02/2014, 13:20

... equation gives the marginal cost of increasing the number of offenses, 0: in equation (21) through a reduction infand in (22) through a reduction in p Since C' > and is assumed to be in a region where ... top also in MR - Number of offenses FIGURE The cost of apprehending and convicting offenders is affected by a variety of forces An increase in the salaries of policemen increases both C' and C,,, ... eliminate all of them and to engage in pure monopoly pricing, in general, however, as with other kinds of offenses, there are two costs of eliminating violations There is first of all the cost of...

Ngày tải lên: 20/02/2014, 19:20

... 0.1% Chaps and 150 mM NaCl and protease inhibitors [5 lgÆmL)1 pepstatin A, lgÆmL)1 aprotinin, and mM phenylmethanesulfonyl fluoride]) and lysed by three cycles of freezing and thawing in liquid ... each of GTP and CTP, mM pyruvate, mM creatine phosphate, 0.2 mgÆmL)1 creatine phosphokinase and 100 lM each of 20 L-amino acids The reaction was initiated by the addition of 200 lCiÆmL)1 of [32P]UTP ... ADP and succinate as described in the Materials and methods section Hexokinase and phosphofructokinase activities were measured in the cytosolic fractions Isocitrate dehydrogenase and NADH:ubiqinone...

Ngày tải lên: 20/02/2014, 23:20

... previously [34]) as a standard The amounts of chromatophores and standard protein in the different lanes of a single gel were kept in the linear range of the luminol assay response Light-induced ATP synthesis ... injecting an acidified chromatophores suspension into a luminometer cuvette containing a basic solution and monitoring the ATP concentration continuously with luciferine/luciferase in the luminometer ... for the experiments in the absence (dashed line) or in the presence (continuous line) of valinomycin As no ATP synthesis could be detected with added valinomycin for short illumination times, the...

Ngày tải lên: 21/02/2014, 03:20

... actions outlined in Section The definitions of the actions are not stated rigorously, but are intended to give the reader an intuitive understanding of their preconditions and effects, and how they ... degenerate case of the NSI action In the case of the NSI action, the speaker and hearer mutually know of a plan that will result in identification of the intended referent, and in the case of the S[ ... illocutionary acts can be defined in terms of the kinds of inferences made, given a semantic analysis of an utterance, facts about mutual knowledge, and general principles of rational behavior Cohen...

Ngày tải lên: 21/02/2014, 20:20

... this final pool of addresses This “global proposal” was discussed at the LACNIC X meeting in May 2007, in the APNIC 24 meeting in New Delhi in September, and in ARIN and RIPE meetings in October ... informed policy-making  Monitor IPv6 readiness, including by monitoring information on national peering points offering IPv6 connectivity, Internet Service Providers offering commercial IPv6 ... Overview of major initiatives in Internet addressing and routing to-date Internet routing and addressing have been revised over the years to support the expansion in the global use of Internet, with...

Ngày tải lên: 06/03/2014, 21:20

... synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into soluble ... amino acids and the necessity to purify each individual sample In contrast, cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and ... containing inexpensive 15 NH4Cl and the [15N]-labelling of selected residues was suppressed by the addition of amino acids at natural isotopic abundance [17] In the case of cell-free protein synthesis,...

Ngày tải lên: 07/03/2014, 12:20

... emulsification in the field of papermaking unique /67/ 37 INTERNAL SIZING OF PAPER AND BOARD The word sizing is used when talking about paper and board hydrophobation Two kinds of sizing; internal sizing and ... emulsions include economy and ease of application, increase in rate and extent of penetration into the skin, possibility of applying both water and oil soluble ingredients simultaneously, and efficient ... with ink-jet printing papers, but is more common in supercalandered papers and especially in liquid packaging boards /3, 4, 77/ 3.2.2 Rosin sizing agents Rosins used in paper and board sizing...

Ngày tải lên: 09/03/2014, 01:20