pi3kinase akt mammalian target of rapamycin pathway

... to produce more of Akt Overstimulation of Akt may lead to enhanced activation of telomerase, and hence continued cell proliferation This may be one of the pathways implicated in rapamycin resistance ... studies of rapamycin are yielding new and interesting mechanisms of action of this drug, outside of its action on mTOR Noteworthy of these are the handful of studies that have reported that rapamycin ... consists of Raptor (Regulatory associated protein of mTOR) and mLST8 (mammalian Lethal with SEC13 protein 8), and mTORC2 consists of Rictor (Rapamycin insensitive companion of mTOR), mSIN1 (mammalian...

Ngày tải lên: 05/10/2015, 13:53

... findings of stronger co-expression of the p110a subunit and mTOR, suggest that pharmacological co-targeting of p110a and mTOR might be a useful strategy for treating RCC Activation of the PI3K -Akt pathway ... Elfiky et al.: Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer Journal of Translational Medicine 2011 9:133 ... patterns of PI3K pathway members critical for cell survival and proliferation in a large cohort of RCC specimens We used a novel method of quantitative immunofluorescence, AQUA This method is void of...

Ngày tải lên: 18/06/2014, 22:20

... findings of stronger co-expression of the p110a subunit and mTOR, suggest that pharmacological co-targeting of p110a and mTOR might be a useful strategy for treating RCC Activation of the PI3K -Akt pathway ... Elfiky et al.: Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer Journal of Translational Medicine 2011 9:133 ... patterns of PI3K pathway members critical for cell survival and proliferation in a large cohort of RCC specimens We used a novel method of quantitative immunofluorescence, AQUA This method is void of...

Ngày tải lên: 20/06/2014, 04:20

... signaling pathway underlying Foxp3+ Tregs expansion in humans has not been well characterized Recent research has demonstrated that inhibition of the mammalian target of rapamycin (mTOR) is capable of ... phosphoinositide-3kinase (PI3K) -Akt- dependent fashion In the presence of rapamycin, the PI3K -Akt- mTOR signaling pathway is inhibited, and multiple downstream targets of mTOR, such as 4E-BP1, are ... Research 2009, 10:13 expressions of PI3K, pAkt, pmTOR, and p4E-BP1 and lower expression of PTEN were observed in nasal polyps compared to the control in the absence of rapamycin stimulation (P < 0.05...

Ngày tải lên: 12/08/2014, 14:20

... 2135 A molecular target of SQAGs A S Aoki et al A B Fig Inhibition of the microtuble depolymerization activity of MCAK184 by SQAG in vitro In all assays, 120 nM of MCAK, 1500 nM of paclitaxel stabilized ... immobilize on the control well Increase rate of recovery for control ¼ titer of SQAG immobilized well ⁄ titer of control well Table SPR analysis of the binding of SQAGs to the immobilized peptide, MCAK184 ... Occurrence of animal sulfolipid in the gametes of sea urchins Jpn J Exp Med 35, 315–318 Isono Y & Nagai Y (1966) Biochemistry of glycolipids of sea urchin gametes I Separation and characterization of...

Ngày tải lên: 19/02/2014, 17:20

... autophosphorylation activity, loss -of- function (kinase-inactive) forms of the MAPKs were produced as GST-lofMPK3 and GSTlofMPK6 However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation ... decipher the specificity of action of each cascade and what mechanisms restrict or regulate cross-talk between distinct pathways Experimental procedures PTI1-4, a common target of OXI1 and MAPKs ence, ... K45R lofMPK3 MPK6 lofMPK6 K45R K45R K45R OXI PTI OXI PTI HIS: - OXI PTI OXI PTI 30 15 30 + + + + + – -72 -55 HIS-OXI1 > < MBP HIS-PTI1-4: 15 30 45 – < HIS-OXI1 GST-MPKs < GST B OXI1 lof lof -...

Ngày tải lên: 14/02/2014, 19:20

... thought of as the description of, e.g the center of gravity of a solid body by so-called barycentric coordinates Yet another legerdemain, suitable for a colorblind person, would perhaps consist of ... number of vertices of the current polytope in Clarke’s exposition and number of basic colors here; r is number of reactions; and n is number of independent species) The number of basic colors, ... surface of the tetrahedron owing to a collapse of the third coordinate In summary, this geometrical illustration should provide a particularly lucid and intuitive picture of the mixing of basic...

Ngày tải lên: 19/02/2014, 13:20

... composition of the extracellular matrix of human oocytes and cumulus masses Hum Reprod 1992, 7:391-398 Dandekar P, Talbot P: Perivitelline space of mammalian oocytes: extracellular matrix of unfertilized ... adult females In the group of young females, but not the adults, development of the embryos was also highly synchronous this was accompanied by increased rate of movement of eggs or microspheres ... evidence supporting the idea that the oviduct is targeted by cigarette smoke (1) In vivo evidence that the oviduct is a target of smoke Direct inhalation of whole smoke has been shown in several studies...

Ngày tải lên: 05/03/2014, 17:20

... mechanisms of PP2A regulation which are independent of the canonical A and B regulatory subunits Type 2A phosphatases are key players in the yeast target of rapamycin (TOR) signaling pathway [3] ... regulator of the rapamycin- sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator of the activity of type 2A ... Therefore, characterization of TOR signaling pathway regulator-like (TIPRL; TIP41), the mammalian ortholog of Tip41, may provide clues to better understand the regulation of type 2A phosphatases...

Ngày tải lên: 07/03/2014, 05:20

... al three putative categories of targets, Six1-specific targets, Six4-specific targets and common targets We then focused our analysis on one of the typical common target genes, sodium-potassium-chloride ... Six4W263R were considered potential Six4 target genes A total of 363 Six1 target genes and 149 Six4 targets genes were identified Of these, 63 were common target genes The data were deposited in ... regions [16] To explore the target genes of Six1 and Six4 in the physiological context of development, we took advantage of using the cell line mK4 isolated from the kidneys of transgenic mice that...

Ngày tải lên: 07/03/2014, 17:20

... membrane interaction of SRP pathway components in Archaea, the membrane binding of SRP54 and FtsY in the halophilic archaea Haloferax volcanii was investigated Such studies offer insight not only ... polyhistidine-tagged version of the protein Given that the presence of a His6-tag at the C-terminus of the E coli FtsY NG domain did not interfere with determination of the 3D structure of this FtsY region ... confirmed the presence of clusters of positively charged residues within the first 46 residues of the A domain (Fig 3), a length of FtsY shown to be important for membrane localization of the protein...

Ngày tải lên: 16/03/2014, 16:20

... al SerpinA3 is a novel target of Nur77 Table Information of candidate genes with NBRE in the promoter regions NBRE position, the position of the first and the last base of NBRE to TSS; direction: ... SerpinA3 is a novel target of Nur77 Y Zhao et al A B C Fig Structure and nucleotide sequences of the proximal promoter region of human SerpinA3 (A) Schematic presentation of the 5¢ SerpinA3 promoter ... and protein levels of SerpinA3 were increased; when the expression of Nur77 was suppressed, the expression of SerpinA3 was reduced Second, analyses of the regulatory elements of SerpinA3 demonstrated...

Ngày tải lên: 23/03/2014, 07:20

... direct target gene of CUX1 Expression analyses show a reciprocal expression pattern of CUX1 and PLZF among CRC cell lines and normal isolated colonocytes Because of the well-documented role of PLZF ... with the production of multiple isoforms of the proteins, as reported previously (Fig 4B) [35] The murine Cux1 protein was shorter than the human CUX1 protein because of the use of an N-terminally ... [11] Several Cux1 isoforms are produced from the proteolytic processing of p200Cux1 (e.g p110Cux1) as well as alternative splicing (e.g p75Cux1) [12] Many in vitro gene targets of CUX1 are implicated...

Ngày tải lên: 29/03/2014, 21:20

Ngày tải lên: 06/06/2014, 13:31

... amplification was done in 50 μL of PCR mixture containing μL of the cDNA mixture, μL of KOD Plus DNA polymerase (TOYOBO, Osaka, Japan) and 15 pmol of primers For specific detection of AMACR, forward primer ... http://www.translational-medicine.com/content/7/1/103 Figure Expression profiles of AMACR as assessed by RT-PCR Expression profiles of AMACR as assessed by RT-PCR A Expression of AMACR in normal tissues including heart, brain, ... profile of AMACR in normal adult tissues by RT-PCR was difined We detected the overt expression of β-actin mRNA and AMACR mRNA in prostate cancer line LNCaP, but only very weak expression of...

Ngày tải lên: 18/06/2014, 15:20

... in cell culture)[22,23] The results of our recent study indicated a strong proof of the reliability of iTRAQ Page of 10 approach in the proteomics analysis of PCa Periostin as an up-regulated ... application Based on the condition of screening differentially expressed proteins(the fold Page of 10 change cutoff ratio1.50 as criterion to identify proteins of differential expression (P ... expression and function of Periostin in PCa The expression of Periostin was verificated by western blotting The results revealed a significant increase of the amount of Periostin in PCa compared...

Ngày tải lên: 18/06/2014, 19:20

... in cell culture)[22,23] The results of our recent study indicated a strong proof of the reliability of iTRAQ Page of 10 approach in the proteomics analysis of PCa Periostin as an up-regulated ... application Based on the condition of screening differentially expressed proteins(the fold Page of 10 change cutoff ratio1.50 as criterion to identify proteins of differential expression (P ... expression and function of Periostin in PCa The expression of Periostin was verificated by western blotting The results revealed a significant increase of the amount of Periostin in PCa compared...

Ngày tải lên: 20/06/2014, 04:20

... distributed along the full length of the molecules All of these epitopes share basic properties and are exposed on the surface of the protein, rendering them antigenic [19] One of the B-cell epitopes on ... carboxyl-terminus of SmD1 [21,22] R20 The aims of the present study were to develop a peptidebased ELISA system for the detection of anti-Sm antibodies and to evaluate the diagnostic properties of the peptide ... instructions for use Blood donors exhibited a reactivity range of 0.4–11.5 U/ml, a mean value of 2.2 U/ml and a standard deviation of 1.2 U/ml The cutoff was set at 13 U/ml following receiver operating...

Ngày tải lên: 09/08/2014, 06:22

... specificity of clustering was similar and low, regardless of the methods Other pathways and systems were confidently clustered regardless of the protocol These include, for example, the MEP pathway of ... percentage of lost data (b) The effect of selected distance measures between phyletic patterns on the recovery of functionally linked pairs of genes The criteria of functional linkages on the basis of ... and degradation of all classes of molecules, signal transduction, cell division, and so on - and was especially evident in the case of long biosynthetic pathways Indeed, of the 52 pathways represented...

Ngày tải lên: 09/08/2014, 20:20

... to 145-fold (KYNU) Modulation of transcript-levels by IFN-g and/or TNF-a The potential effects of IFN-g, TNF-a, or a combination of IFN-g and TNF-a on kynurenine pathway transcripts were investigated ... http://www.journal-inflammation.com/content/8/1/25 Page of Figure Relative levels of transcripts encoding enzymes in the kynurenine pathway (A-J) following treatment with IFN-g (200 U/ml), TNF-a (100 U/ml) or the combination of these two ... potential functionality of the KP in these human fibroblast cultures, we measured the accumulation of KYNA, one of the end metabolites in the KP in the supernatants Levels of KYNA were detectable...

Ngày tải lên: 11/08/2014, 03:20