physiology of skeletal muscle contraction and relaxation

... the investigation of activity [27], the creation of antibody chimeras [28], and the study of Table The ratios of the volumes (V) of the models built by SAXS and of the structure of TnC(1–91) (1SKT) ... structure of TnI and TnC that can explain how they interact during muscle contraction Figure shows a schematic representation of the structural disposition of TnC and TnI in the light of recent ... with excitation at 280 nm and a bandpass of nm, and emission at 320 nm with a bandpass of nm All spectra were baseline corrected with the buffer and were the means of at least three independent...

Ngày tải lên: 16/03/2014, 18:20

... a2') and (m2" a2") induce the same or a different response of the muscle (power output, force and speed of contraction) In this work we try to answer the question by applying a model of muscle contraction ... state of the contraction increases We conclude that a rigorous definition of muscle contraction requires the acceleration of the load to be known and that, at constant load force, the decrease of ... the acceleration of the load slows down the pre-steady state of the contraction Methods The evaluation of the effect of load acceleration on the pre-steady state of muscle fibre contraction was...

Ngày tải lên: 13/08/2014, 16:20

... accumulation of K+ in the t-tubules and interfibre spaces of the muscle LFF is characterized by a relative loss of force at low frequencies of stimulation and a slow recovery over the course of hours ... contribute in the diagnostics of the functional condition of the skeletal muscles and correlate it with certain criteria of the classical diagnostics Simultaneous measurements of intramuscular pressure ... of the skeletal muscle may provide new insights into muscle function and can help to diagnose the stage of pathological processes of muscles grip The testing end (mass 20 grams) of the computerized...

Ngày tải lên: 20/06/2014, 00:20

... involved in the maintenance of skeletal muscle excitability and comprises subunits (α and β), each of which express multiple isoforms at a protein level in skeletal muscle (α1-3 and β1-3) The fibre-specific ... skeletal muscle, six isoforms (α1-3, β1-3) of the NKA are expressed The basic function of each isoform is listed below in Table 1.1 Table 1.1 Basic functions of the NKA isoforms in skeletal muscle ... functions of NKA isoforms Understanding of the specific functions of the NKA isoforms is incomplete In skeletal muscle the α1 isoform plays appears to play a principal role in Na+/K+ transport and...

Ngày tải lên: 04/12/2015, 13:59

... the parameters of IC 50 ¼ 5.65 lM and nHill ¼ 4.71 Number of experiments is shown at each point 465 Effect of gadolinium on RyR1 tion of 5.6 ± 0.3 lm for Gd3+ and a Hill coefficient of 4.7 ± 0.8 ... regulated by several cytoplasmic ligands It is activated by millimolar concentrations of ATP and micromolar Ca2+, and inhibited by higher concentrations (> 100 lm) of Ca2+ and by millimolar Mg2+ Calmodulin ... parameters of IC50 ¼ 5.47 lM and nHill ¼ 4.31 Number of experiments is shown at each point Table The effect of cis gadolinium on the open time constant of RyR1 T, Open time constant ± SE; N, number of...

Ngày tải lên: 19/02/2014, 16:20

... checked at the time of muscle excision by visualization of abnormal gait of the limb and by verifying the discontinuity of the sciatic nerve at the thigh The TA, EDL and soleus muscles were sampled ... forming the muscle differs between muscles: the quadriceps muscle is composed roughly of 50% type fibres and 50% type fibres, the TA and EDL muscles contain about 5–10% type fibres, the soleus muscle ... protein in muscle plasticity Fig Analysis of CARP protein level and cellular localization in denervation-induced atrophy and in murine models of MD (A) In conditions of both transient (T) and definitive...

Ngày tải lên: 07/03/2014, 03:20

... intensity of ¯uorescence of epsilon nucleotides bound to G- and F-actins Fig shows changes in the relative intensity of ¯uorescence of eATP-G-actin and eADP±F-actin in the presence of eATP as ... nm of eATPG-actin was higher than that of eADP±F-actin Both actins and eATP buffer showed an increase in intensity of ¯uorescence when exposed to a pressure of 250 MPa However, the increase of ... Pressure-induced denaturation of actin (Eur J Biochem 269) 365 The aim of the present study was to complete a study of F ® G transition and denaturation of actin under pressure Use of a Hitachi F2000 ¯uorospectrophotometer...

Ngày tải lên: 24/03/2014, 00:21

... E2-14KDa, and C2, myofibrillar and sarcoplasmic muscle proteins were prepared as described previously [2] Myofibrillar and sarcoplasmic muscle protein were separated on SDS-polyacrylamide gels and ... longus muscle The proteolytic rate of skeletal muscle was measured as net release of tyrosine for total protein and 3-MH for myofibrillar protein Compared to the control group, the rate of total ... activity of the Ub system, the release of tyrosine and 3-MH in EDL also decreased These findings, to some extent, suggest that infusion of insulin alleviates degradation of skeletal muscle protein...

Ngày tải lên: 11/08/2014, 06:23

... decreased in the skeletal muscle of climbers acclimatizing to high altitude [12,13], and was found to be lower in the muscles of Himalayan Sherpas than those of unacclimatized lowlanders [13] Prolonged ... muscle and stimulate mitochondrial biogenesis via the activation of a number of transcriptional pathways [20,21] Overexpression of PGC-1α in mouse skeletal muscle leads to a proliferation of fatigue-resistant ... [27] In the skeletal muscle of lowlanders acclimatized to 4,559 m, levels of mTOR were decreased [28], possibly indicating a suppression of mitochondrial biogenesis via downregulation of PGC-1α...

Ngày tải lên: 11/08/2014, 12:20

... laboratory, and groups of mice We also recognize that the behavior of the microcirculation in the EDL skeletal muscle during sepsis may not be representative of the microcirculation of other tissues ... dysfunction and tissue dysoxia [34] Based on these observations, we tested the hypothesis that rHuEPO given as a single dose of 400 U/kg would improve skeletal muscle microcirculation and tissue Page of ... observing capillaries of the EDL and counting the number of perfused and stopped capillaries crossing three equidistant lines drawn perpendicular to the direction of the muscle fibers on the observation...

Ngày tải lên: 13/08/2014, 03:21

... ratio, and a decrease in the activity of CK and some of its isoforms in several different forms of cancer [15–20] Skeletal muscle tissue contains high levels of phosphocreatine, creatine and CK, and ... Immunoblot of CK isoforms of different post-operative human tissues (A) Immunoblot of MCK (a) and sMitCK (b) of normal muscle (N) and fibrosarcoma (T) tissue (B) Immunoblot of BCK (a) and uMitCK (b) of ... striated skeletal and heart muscles and ubiquitous uMitCK is present in smooth muscle, brain, neuronal and other non -muscle Table Creatine and phosphocreatine content in normal muscle (NM) and sarcoma...

Ngày tải lên: 07/03/2014, 06:20

... The effects of tigrin and ablomin on rat tail arterial contraction Fig Isolation of tigrin from the Duvernoy’s glands of R tigrinus tigrinus (A) The extract from Duvernoy’s glands of R tigrinus ... obtained mg of triflin and mg of latisemin from 500 mg of crude venom, respectively The predicted amino-acid sequences of triflin and latisemin are homologous to that of ablomin (83.7 and 61.5%, ... shown on the left Lanes and show ablomin (26 and 29.7 kDa), lanes and show triflin (23 and 29 kDa), lanes and show latisemin (28 kDa each), and lanes and show tigrin (28 and 30 kDa) The numbers...

Ngày tải lên: 18/03/2014, 01:20

... identified as a partner of skeletal muscle titin-N2A [18] An analysis of CARP expression in the subcellular compartments Regulation of CARP by calpain obtained from the muscle of the mice injected ... acids 65 and 88 and the cleavage site is localized before amino acid 71, the region of cleavage would be between amino acids 65 and 71 Considering the location of the N-terminal extremity of DNter2 ... structure and their potential role in the control of transcription [17,19–21] Although the three MARPs are expressed in both heart and skeletal muscle [18,22,23], Ankrd2 is mainly expressed in skeletal...

Ngày tải lên: 29/03/2014, 21:20

... with a ramp filter and a cutoff frequency of 0.5 of the Nyquist frequency to obtain an image pixel size of 0.4 × 0.4 × 0.8 mm and an inter-plane spacing and slice thickness of 0.8 mm in a 128 ... i for myocardium and skeletal muscle and possibly related to inhibitory effects of high plasma free fatty acid concentrations on tissue uptake and a low membranous expression of GLUT4 The marked ... regarding glucose metabolism between heart and skeletal muscle, indicate that myocardium and skeletal muscle might have different mechanisms for regulation of glucose or FDG uptake in response to...

Ngày tải lên: 21/06/2014, 02:20

... global molecular profiling in the skeletal muscle of cancer patients and relate our findings to clinical status Page of 12 Methods Men and non-pregnant women over 18 years of age were recruited ... demonstrate lack of relationship between weight loss and muscle damage, muscle sepsis and exercise training status The top 20 most regulated genes by (a) eccentric muscle damage, (b) muscle obtained ... in muscle and liver samples in cancer-related cachexia J Mol Med 2007, 85:647-654 10 Mammucari C, Schiaffino S, Sandri M: Downstream of Akt: FoxO3 and mTOR in the regulation of autophagy in skeletal...

Ngày tải lên: 11/08/2014, 12:20

... predictors of mortality [4,5] and patients who failed to gain weight after a program of nutritional support also had a worse prognosis [6] Skeletal muscle is a major component of fat free mass and skeletal ... investigated changes in fat free mass and skeletal muscle strength in a cohort of patients with COPD over one year's follow up Skeletal muscle depletion was common at baseline and was associated with a more ... the other hand it has the merit of incorporating an element of 'clinical significance' Other definitions and techniques such as diary cards have been used and this remains an area of controversy,...

Ngày tải lên: 12/08/2014, 15:20

... of Rho-kinase inhibition on prostaglandin-induced contraction Effects of Rho-kinase inhibition on prostaglandin-induced contraction PGE2 (A) -and PGF2α (B)-induced contraction in the absence and ... EGF (A) -and PDGF (B)-induced contraction of guinea pig open-ring tracheal smooth muscle preparations EGF (A) -and PDGF (B)-induced contraction of guinea pig open-ring tracheal smooth muscle preparations ... EGF and PDGF induce airway smooth muscle contraction through contractile prostaglandins These prostaglandins are presumably produced by the consecutive actions of MEK, cytosolic PLA2 and COX and...

Ngày tải lên: 12/08/2014, 18:22

... of the experiment, tissue samples were taken for isolation of mitochondria from the liver, muscle and kidney For technical reasons, one sample of the liver and two samples from the skeletal muscle ... design and the in vivo experiments, carried out the isolation of the mitochondria and wrote the manuscript JT participated in the study design and the analysis and interpretation of the results, and ... redox state and microcirculation Materials and methods The study was performed in accordance with the National Institutes of Health guidelines for the care and use of experimental animals and with...

Ngày tải lên: 13/08/2014, 02:24

... myotubes and L6 myotube protocols v Abstract Nolan John Hoffman THE EFFECTS OF CHROMIUM ON SKELETAL MUSCLE MEMBRANE/CYTOSKELETAL PARAMETERS AND INSULIN SENSITIVITY A recent review of randomized ... turning off glycogenolysis and gluconeogenesis In adipose tissue and striated muscle (i.e skeletal and cardiac muscle) insulin signals to stimulate glucose transport out of the bloodstream and into ... eight weeks of HF feeding As observed in other insulin-resistant animal models, skeletal muscle from HF-fed mice displayed membrane cholesterol accrual and loss of F-actin Skeletal muscle from...

Ngày tải lên: 24/08/2014, 12:27

... comparison of Tm5a with smTm allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscle exon 1b, and comparison of Tm5a with Tm5b allows the effects of exchange of exon to ... unfolding of homo- and heterodimers of skeletal [15,16] and smooth [17–19] muscle Tm, their mutants [20–23], and numerous coiled-coil model peptides corresponding to the N- and C-terminal parts of the ... exon 9d Both of the fibroblast isoforms are shorter than smTm because they lack exon due to replacement of muscle exons 1a and 2a by nonmuscle exon 1b The short fibroblast isoforms Tm5a and Tm5b differ...

Ngày tải lên: 19/02/2014, 07:20