phosphatase activity of pp2a
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... measurement of heme content is useful for evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free heme in expression of HO-1 To evaluate ... specific inhibitor of d-aminolevulinic acid dehydratase, the second enzyme of the heme biosynthetic pathway SnPP is a competitive inhibitor of HO activity [35] The heme content of all three cell ... expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular heme contents...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... independent of its cysteine protease inhibitor activity [23] Thus, it is clear that cystatin C has various effects on the cell Some of these seem to be independent of its cysteine protease inhibitory activity ... negative controls, instead of cell lysate, 10 lL of 0.10 lm E-64 or 10 lL of 0.01% Brij-35 was used The mixture was allowed to incubate for 10 before the addition of lL of substrate (Z-Phe-Arg-AMC; ... easily defined group of cells that could be gated (Fig 1A) Typically < 15% of the events were excluded The reproducibility of the experiments was high and internalization of the labelled protein...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... PMA-stimulated PMNs, with or without inhibition of NADPH oxidase as an index of translocation of p47phox The generation of O2– by PMNs is attributed to the activation of a plasma membrane NADPH oxidase The ... compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell viability of PMNs PMNs maintained about 80% viability in control ... 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 PMNs 120 100 80 60 Selenourea...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... a factor of 9.7) Thus, the background in the off-state was much lower with pEBTetD than with pEBTet However, in contrast to expression of membrane proteins, the level of expression of cytosolic ... testing of individual clones In contrast, plasmids with an Epstein–Barr virus (EBV) origin of replication oriP in the presence of EBV-encoded EBNA-1 protein are continuously propagated in 1% of initially ... the off-state generate, because of highly efficient translation, considerable amounts of protein A 100-fold increase in mRNA level does not increase protein in an equivalent proportion because of...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia on expression of heme oxygenase (HO)-1 ... BSA, 30 lg of biliverdin reductase, and 15 lg of cytochrome P450 reductase After of preincubation at 37 °C, the reaction was started by addition of 10 lL of NADPH (8.4 lgÆmL) or 10 lL of water ... mouse liver after days of normobaric hypoxia (10% oxygen) and returned to the basal level after 14 days of hypoxia [26] It is therefore of significance to study the regulation of HO-2 expression in...
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Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc
... design of the study and the analysis of the results Author details Division of Clinical Onco-Immunology, Ludwig Center for Cancer Research of the University of Lausanne, Switzerland 2Department of ... Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) Brussels, Belgium 4Department of ... variability, the expression of some of these miRNAs was found to be preferentially associated with specific subsets of CD8+ T cell differentiation Interestingly, the expression level of miR-20a was significantly...
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báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx
... design of the study and the analysis of the results Author details Division of Clinical Onco-Immunology, Ludwig Center for Cancer Research of the University of Lausanne, Switzerland 2Department of ... Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) Brussels, Belgium 4Department of ... variability, the expression of some of these miRNAs was found to be preferentially associated with specific subsets of CD8+ T cell differentiation Interestingly, the expression level of miR-20a was significantly...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
... [17–19] PP1 also inhibited activation of RAS stimulated by convulxin, consistent with the role of these kinases in mediation of the phosphorylation of the ITAM motif of the Fc receptor c chain [11,12] ... number of other cells, PKC is also able to regulate ERK activity In most of these cases, PKC activates RAF, which in turn activates the MEK–ERK cascade, but activation of ERK without involvement of ... which activation of PtdIns3K by TPO potentiates activation of MEK induced by thrombin, which in turn potentiates activation of ERK [23] Whether this set of events involves activation of RAS is not...
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Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot
... after up to of incubation with WCE These results suggest the involvement of PARP-1 and PARP-1 poly(ADP-ribosyl)ation during BER of a uracil-containing oligonucleotide Inhibition of PARP-1 poly(ADP-ribosyl)ation ... simply 2013 Role of PARP-1 in base excision repair A B J L Parsons et al binding of BER proteins We found that 3-aminobenzamide stimulated cross-linking of PARP-1 throughout the course of the reaction ... several cycles of PARP-1 binding and dissociation We further speculate that this repetitive binding of PARP-1 is required only when the number of unrepaired strand breaks exceeds the amount of rate-limiting...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
... Differential stabilization of p53 by VRK2 isoforms Fig Expression of two VRK2 isoforms (A) Detection of VRK2 proteins in several cell lines that were determined by immunoblotting of whole-cell extracts ... stabilization of p53 by VRK2 isoforms S Blanco et al Fig Stabilization of endogenous p53 and activation of p53dependent transcription (A) Stabilization of endogenous p53 by the VRK2B isoform in A549 ... anti-HA serum (Upper) Levels of p53 in the absence of VRK2B, (lower) levels of p53 in the presence of VRK2B (B) Activation of endogenous p53-dependent transcriptional activity by VRK2B A549 cells...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
... Modfit LT software (Verity) Statistical analysis IC50 values were calculated using CalcuSyn software (BioSoft) For Lox-IMVI, combination index (CI) values were calculated using CalcuSyn software ... inhibition of proliferation compared to either drug alone The combination of taxotere and dasatinib also significantly increased inhibition of proliferation compared to either drug alone Page of 11 ... cells Concentrations of temozolomide are represented as a ratio of the dasatinib concentration Error bars represent the standard deviation of triplicate experiments Page of 11 (page number not...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... of 12 0.051; out of 20 0.017; out of 59 0.062; out of 16 0.039; out of 25 0.165; out of Also available online at http://ccr.coriell.org/Sections/Support/Global/QCgenotype.aspx?PgId=412 Page of ... 6:75 identified at the boundary of the wound bed and the rim of necrotic or infected tissue to be removed This area is often identified by a callus After biopsy of Location B, a sharp excision ... as measurement of growth factor production and response, expression of cell cycle proteins, and cell morphology, hold a piece of the puzzle as to why certain wounds not heal Part of the challenge...
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Báo cáo sinh học: " Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... Research Page of 10 10 11 12 13 14 Author details Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, 68105 USA 2Department of Oncology, Zhongnan Hospital of Wuhan ... the cytotoxicity of SU11274, which might be due to an increased phosphorylation level of c-Met caused by HGF We tested the effects of SU11274 and HGF on the phosphorylation level of c-Met in RMS ... Hou et al Journal of Translational Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure Analysis of the expression and localization of phosphorylated c-MET...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... suppressor of malignant melanoma cell growth, further promoting evidence of expanding the testing of the role of AMPK activation for melanoma treatment Metformin is a derivative of guanidine, one of ... therefore inactivation of the ACC1 isoform of acetyl CoA carboxylase (ACC) [12] or suppression of lipogenic gene expression, including ACC1 and fatty acid synthase, and increase of fatty acid oxidation ... on the single agent activity of each one of them [14,16,17] In our studies, the combination of vemurafenib and metformin synergistically inhibited proliferation in a subset of human melanoma cell...
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Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
... stages Journal Of Virology 1982, 43:519-528 Gravell M, Granoff A: Virus and renal adenocarcinoma of Rana pipiens: IX The influence of temperature and host cell on replication of frog polyhedral ... elimination of cryptic splice sites, and the disruption of some regulatory elements that normally may result in a decrease in protein production A comparison of the original nucleotide sequence of 75L ... http://www.virologyj.com/content/5/1/83 ient of a NSERC postgraduate scholarship We thank Dr V.G Chinchar of the University of Mississippi Medical Center for providing the anti-FV3 antibodies References The consequence of codon...
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báo cáo hóa học:" Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... Research Page of 10 10 11 12 13 14 Author details Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, 68105 USA 2Department of Oncology, Zhongnan Hospital of Wuhan ... the cytotoxicity of SU11274, which might be due to an increased phosphorylation level of c-Met caused by HGF We tested the effects of SU11274 and HGF on the phosphorylation level of c-Met in RMS ... Hou et al Journal of Translational Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure Analysis of the expression and localization of phosphorylated c-MET...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... numbers of quantitative trait loci underlying each type of phenotype, but few examples of individual loci driving variation in both methylation and expression To better understand the role of genetic ... patterns of methylation Distinct patterns of methylation were observed for CpGsites located on the autosomes, X-chromosome, and in the vicinity of imprinted genes (Figure 1a) The majority (71.4%) of ... of 10% (P = 2.1 × 10-10) methylation levels at 37 CpG-sites showed evidence for association with Page of 13 SNP genotypes (Table S2 in Additional file 1) The majority of these CpG-sites (27 of...
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Báo cáo y học: "LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cell" pps
... inhibition of MCP-1 is secondary to the inhibition of TNF by LMP-420, since TNF can itself stimulate release of MCP-1 This pattern of cytokine/ chemokine inhibition is, of course, only representative of ... Inhibition of TNF release can occur at a number of different points in stimulated cells These might include initial binding of the stimulating ligand, transcription of TNF mRNA, translation of mRNA ... replication of M Tb Engele et al [8] demonstrated that infection of human alveolar macrophage (AM) with virulent strains of mycobacteria induced the secretion of significantly higher levels of TNF...
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