phosphatase activity of pp2a

... bound per H4-H5 loop or per the a subunit of Na+/K+ ATPase [17] K+ activated phosphatase activity of the ATPase as well as the phosphatase activity of the H4-H5 loop protein were tested as described ... optimized Results Effects of truncation of the cytoplasmic H4-H5 loop of the a subunit of Na+/K+ ATPase on TNP-ATP binding and p-nitrophenylphosphatase activity Molecular modeling of the H4-H5 loop according ... dependent p-nitrophenylphosphatase activity Table TNP-ATP binding to and p-nitrophenylphosphatase of H4-H5 loop–GST fusion proteins of different lengths The increase of the intensity of fluorescence by...

Ngày tải lên: 19/02/2014, 16:20

... decreased amounts of nuclear PTEN These changes in the abundance of nuclear PTEN may reflect the regulation of conformations of PTEN in these cells Moreover, various effects of substitution of lysines ... Plus molecular imager (Bio-Rad) Phosphatase activity.   The phosphatase activity of PTEN was measured, as described previously30,37 Wild-type and mutant forms of PTEN fused to GFP were expressed ... higher than that of PTENA4 (Fig.  3C,D) Therefore, K13 likely regulates the stability of open conformation PTENs Previous studies have suggested that the loss of the lipid phosphatase activity stabilizes...

Ngày tải lên: 04/12/2022, 16:02

... of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed. Microsensors For analysis of microbial structure and function (activity) of ... only net chemical profiles, and the spatial resolution is also above a single-cell level. To address the question of the higher abundance and activity of SRB in oxic zones of biofilms (Okabe et ... types of biofilms, a domestic wastewater biofilm and an autotrophic nitrifying biofilm, were studied. Both biofilms were cultured in partially submerged rotating disk reactors (RDR) consisting of...

Ngày tải lên: 05/09/2013, 08:40

... structure of the available inhibitors of cathepsin D [19], a lysosomal protease of mammalian cells, and chalcones and phenothiazines have been assayed as inhibitors of falcipain-2 [20,21] Inhibition of ... Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of proteases of T gondii [14] and HIV [12,31] To ascertain the inhibition range of MA on the different ... proteolytic hydrolysis of haemoglobin as a source of amino acids constitutes one of the essential processes which take place along the intra-erythrocytic stage of the parasite Degradation of haemoglobin...

Ngày tải lên: 14/02/2014, 14:20

... hydrolytic activity towards ATP (Fig 1A) The binding of a magnesium cofactor has long been established as essential for the activity of canonical GTP-binding proteins [29,31] The precise role of Mg2+ ... determine the requirement for magnesium of the GTPase activity of TorD, the reaction was performed in varying amounts of MgCl2 (Fig 1B) The GTPase activity of TorD without added MgCl2 is negligible, ... co-ordination of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12] The concentration of GTP in the cytoplasm of...

Ngày tải lên: 16/02/2014, 09:20

... activity by measuring degradation of the Ac-DEVD-pNA peptide (right panel) The graphs represent average cell survival as a percentage of the control and the average fold increase of caspase activity ... induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngỈmL)1 TRAIL for h led to a significant loss of cell viability (33.1 ± 3.3% of control; ... cells were cotransfected with constant amounts of rLuc–Itch and various amounts of either Bid–GFP of tBid–GFP The graph is a representative example of the saturation studies performed to provide...

Ngày tải lên: 16/02/2014, 09:20

... detected in the case of mGFP–calpain 7C290S, suggesting that the degradation was caused by proteolytic activity of mGFP–calpain itself We refer to this issue later Effects of mutations of IST1 MIMs on ... interaction enhances the autolytic activity of mGFP– calpain 7–Strep in vitro Autolytic properties of mGFP–calpain 7–Strep We further characterized the autolytic activity of mGFP–calpain 7–Strep Purified ... but not by GST–MIML326D,L353A or GST Enhancement of autolytic activity of mGFP– calpain by calpain 7–IST1 interaction As shown in Fig 3, expression of mGFP–calpain in HEK293T cells generated  45...

Ngày tải lên: 18/02/2014, 04:20

... dramatically reduced activity The role of dimer interface residues in the stability and activity of the Plasmodium falciparum enzyme, PfTIM, has been probed by analysis of mutational effects ... presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74 Surprisingly, the Y74W mutant exhibited loss of both activity ... the progress of the reactions of WT enzyme and other mutants is very fast The results are summarized in Fig It is evident that the Y74W* mutant shows an enhancement of activity of 21.9-fold over...

Ngày tải lên: 18/02/2014, 11:20

... chaperone activity of aB-crystallin [27], which was reported to occur via enhancement of the dynamics of subunit assembly [28] However, to date there have been no reports of the effects of such ... Results The effect of Arg-HCl on the chaperone activity of aB-crystallin is target protein-specific In order to investigate the effect of Arg-HCl on the chaperone activity of aB-crystallin, we ... ability of aB-crystallin in the presence of that concentration of the additive amount of DTT-induced aggregation of a-lactalbumin was increased in a concentration-dependent manner by the addition of...

Ngày tải lên: 18/02/2014, 16:20

... these data are suggestive of a role of membranes, in particular of negatively charged membranes, in the mechanism of action of RNases, we investigated the ability of a series of RNases to destabilize ... shows the shape of the electrostatic field of BS-RNase seen from different directions Figure S3 compares the electrostatic field of different Fig Plot of the EIE values as function of rotation (r) ... cycles of steepest descent followed by 50 cycles of conjugate gradients and 50 cycles of steepest descent) No clashes were detected at the dimer interface of the models or in the surroundings of...

Ngày tải lên: 19/02/2014, 06:20

... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... reaction of OsDmc1 (1) without competitor; (2) with 27.5 l M unlabeled of Phi- C along with 27.5 l M of rhodamine labeled Phi-C; (3) with 55.0 lM unlabeled of Phi-C along with 27.5 lM of rhodamine ... dependence of renaturation activity In the following experiment, we examined the homol- ogy dependence of the renaturation reaction. FRET efficiency was assessed as a function of different doses of unlabeled...

Ngày tải lên: 19/02/2014, 07:20

... of increasing concentrations of native and oxidized elafin and trappin on the activity of a constant concentration of NE and kdiss Ki ¼ kdiss ⁄ kass [5,8] Trappin is a 9.9-kDa protein formed of ... prevents easy binding of the methionine sulfoxide residue at the S1¢ subsite of the active centers of NE and Pr3 The fact that the S1¢ subsite of Pr3 is significantly smaller than that of NE [21] might ... of proteins are susceptible to oxidation by reactive oxygen species Methionine, the most sensitive of amino acids to oxidation, is readily transformed into a mixture of the S- and R-epimers of...

Ngày tải lên: 19/02/2014, 07:20

... Substitution of Arg48 or Arg213 with Ala lowered the activity of RTA 10-fold. Furthermore, substitution of Arg213 with Asp lowered the activity of RTA 100-fold. The crystal structure of this RTA ... Table 1. Assay of the N -glycosidase activity of ricin A chain variants The activity of each of the RTA variants was determined by assessing their ability to depurinate 26S rRNA of Table 1. Data ... effect of substitutions made at each of the arginyl residues on catalytic activity of RTA, the ability of each of the purified RTA variants to depurinate yeast ribosomes was compared to that of wild- type...

Ngày tải lên: 19/02/2014, 12:20

... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... constant of most of the amide protons was in the range of 6–8 Hz, suggestive of rapidly interconverting conformers that coexist in solution The temperature coefficients of chemical shift of amides ... topologies of the turn region mimic those of rat CD2 Structure of cyclic hexapeptides – cEL peptide The chemical shifts of amide resonances of peptide cEL were well dispersed over a region of 1.2...

Ngày tải lên: 19/02/2014, 13:20

... the transport activity were normalized for the surface protein of these mutants Double N-glycosylation mutants showed a marked reduction of GABA-uptake activity of 60–40% of that of the wild type ... N-Glycosylation mutations result in reduction of GABA-uptake activity For quantitative measurement of the specific activity of GABA-uptake, an aliquot of the stable CHO transfectants was used for ... reduced stability of the N-glycosylation mutants of GAT1 must be a result of misfolding of these proteins The deficiency of N-glycosylation results in a markedly reduced GABA-uptake activity (Fig...

Ngày tải lên: 19/02/2014, 17:20

... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalytic power of enzymes and the role of ... k cat and bk cat are consistent with titration of the catalytic histidine, H438(440). The persistence of activity in the presence of the protonated form of the catalytic histidine is inconsistent ... pK a of 4.63 suggests the involvement of an acidic residue in the activity at low pH. Low-pH activity and residue E197 Selwood et al. [34] reported two pK a values for the pH dependence of the...

Ngày tải lên: 20/02/2014, 23:20

... measurement of heme content is useful for evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free heme in expression of HO-1 To evaluate ... specific inhibitor of d-aminolevulinic acid dehydratase, the second enzyme of the heme biosynthetic pathway SnPP is a competitive inhibitor of HO activity [35] The heme content of all three cell ... expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular heme contents...

Ngày tải lên: 19/02/2014, 05:20

... independent of its cysteine protease inhibitor activity [23] Thus, it is clear that cystatin C has various effects on the cell Some of these seem to be independent of its cysteine protease inhibitory activity ... negative controls, instead of cell lysate, 10 lL of 0.10 lm E-64 or 10 lL of 0.01% Brij-35 was used The mixture was allowed to incubate for 10 before the addition of lL of substrate (Z-Phe-Arg-AMC; ... easily defined group of cells that could be gated (Fig 1A) Typically < 15% of the events were excluded The reproducibility of the experiments was high and internalization of the labelled protein...

Ngày tải lên: 16/03/2014, 06:20

... analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia on expression of heme oxygenase (HO)-1 ... BSA, 30 lg of biliverdin reductase, and 15 lg of cytochrome P450 reductase After of preincubation at 37 °C, the reaction was started by addition of 10 lL of NADPH (8.4 lgÆmL) or 10 lL of water ... mouse liver after days of normobaric hypoxia (10% oxygen) and returned to the basal level after 14 days of hypoxia [26] It is therefore of significance to study the regulation of HO-2 expression in...

Ngày tải lên: 19/02/2014, 06:20