pharmacological and chemopreventive studies of chrysanthemum

... characterization of numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided into two domains: a core domain and ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7...

Ngày tải lên: 15/02/2014, 01:20

... A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of His34 ... we report the results of the structural studies of the pantoate-bound form of the N-terminal domain of E coli PS (nPS) The structure and substrate-binding interactions of E coli nPS have been ... specific residues of nPS as a function of substrate(s) The resonances of free nPS are shown in red, those of nPS bound to D-pantoate are shown in blue, and those of nPS bound to D-pantoate and ATP are...

Ngày tải lên: 16/02/2014, 09:20

... possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL [21,34] support ... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... of primary-sequence similarity with pancreatic lipase, and the conservation of most of the disulfide bonds between LPL and PL suggests a similar tertiary structure For the two dimeric models of...

Ngày tải lên: 21/02/2014, 15:20

... NMR studies [16,19,20,23] The energy of folding these various forms and the energetic cost of converting one form to another is of fundamental importance for understanding telomere biology and ... practical details of UV melting studies of G-quadruplexes, with an thorough discussion of common problems and pitfalls [32] The use of fluorescence to monitor the thermal denaturation of quadruplex-forming ... signal for the determination of the fraction of folded or unfolded DNA strand, allowing for the calculation of the equilibrium distribution of conformational forms Standard methods using UV absorbance...

Ngày tải lên: 06/03/2014, 09:22

... calculated from the ratio of the rate constants kon and koff as follows: Ka ẳ kon koff 2ị Association constants (Ka) of CRP with the three investigated sequences of DNA lacF, galF and ICAPF are summarized ... nuclear magnetic resonance study of the cyclic AMP receptor protein (CRP): assignments of the NH protons of histidine and tryptophan residues and the effect of binding of cAMP to CRP Biochem J 107, ... DNA and cAMP i.e free CRP, CRP(cAMP)2 and CRP(cAMP)4 In the presence of % 100 lm cAMP, the protein becomes activated by the formation of a CRP(cAMP)2 complex and it is then able to recognize and...

Ngày tải lên: 07/03/2014, 16:20

... Rao and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS ... molecular mass of 19 216 Da and a pI of 4.69, in good agreement with MALDI-TOF MS (Fig 1A) and chromatofocusing data, respectively The other three N-terminal sequences determined in this work, of Anabaena ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary...

Ngày tải lên: 16/03/2014, 11:20

... turns) and 16% of other contributions for both the native and refolded forms, with a rmsd of 1% The deconvolution of Mfrutalin showed 13% of a helix and 12% of b components (antiparallel and parallel ... where N and U represent the native and reversibly unfolded forms of the frutalin and kN and kU, the equilibrium constants of the unfolding transitions from the N to the U state The fraction of unfolded ... Nfrutalin and Rfrutalin-Ch were deconvoluted by convex constraint analysis [19,20] as described in Materials and methods, and showed 86% of beta components (antiparallel and parallel b sheet, and b...

Ngày tải lên: 17/03/2014, 23:20

... In IB-367 and BM-1, the presence of strong daN(i,i + 1) and weak (or absence of) dMN(i,i + 1) and dbN(i,i + 1) NOE connectivities in the Gly3–Arg8 and Cys14– Gly16 regions of IB-367 and in the ... Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and 4) Our conformational studies showed that BM-1 formed a twisted b-sheet structure, similar to that of native PG-1 and ... activity of the peptides A better understanding of the mode of action of these peptides is crucial for the development of a new generation of antibiotics It is known that, in the absence of both...

Ngày tải lên: 22/03/2014, 21:20

... Lane and 9, respectively, P and S fractions of 0.5% Triton X-100 plus 0.32 M NaCl treatment of thrombin digested microsomes Lane 10 and 11, respectively, P and S fractions of M NaCl treatment of ... respectively Lanes and 5, respectively, P and S fractions of untreated thrombin digested microsomes Lanes and 7, respectively, P and S fractions of 0.5% Triton X-100 treatment of thrombin digested ... mutational studies of chalcone synthase have demonstrated that decarboxylation of malonyl-CoA is independent of acylation of the active site cysteine [24] In these studies, substitution of the active-site...

Ngày tải lên: 24/03/2014, 03:21

... concentration of XAIP (b) and with lM concentration of chitinase enzyme (c) for h, and (B) in the absence of any protein (a), with 100 lM concentration of XAIP (b) and with 100 lM concentration of chitinase ... internal space of the TIM barrel considerably Of the three observed cis peptides, two (Gly33–Phe34 and Trp253–Asp254) are found at the ends of b-strands b2 and b8, respectively These are part of the ... interactions between residues of a7 and those of GH10 because of steric hindrance On the other hand, it has been shown by solution studies that XAIP inhibits the activity of a-amylase in a : 1.2 molar...

Ngày tải lên: 29/03/2014, 09:20

... mechanism of the AspB-catalyzed reaction and pKa values of potential acid and base catalysts by pH-rate studies From the ascending limb of the pH-rate prole of AspB, a residue with a pKa of 7.1 ... obtained from grafit software Kinetics of reversible inhibition of AspB, T141A and H188A by 3-nitropropionate and D-malate The reversible inhibition of AspB and the T141A and H188A mutants was ... pH-rate and inhibition studies to further illuminate the mechanistic role of certain residues The results obtained in these studies are interpreted and related to the mechanisms of AspA and other...

Ngày tải lên: 29/03/2014, 23:20

... reports as well [31] The XPS data of Zn2p and Mn2p further provide evidences for the incorporation of Mn2+ ions into ZnO To obtain a better understanding of the formation of Mn-doped ZnO HMSs, a probable ... coordination route of EG which directs the formation of Mn-doped ZnO HMSs in view of morphology transformation A lot of EG molecules are adsorbed on the (001) surface of the ZnO crystals and block the ... spectra The origin of the peaks at 425 and 475 nm could be ascribed due to the transition occurring from Zn interstitials to the valence band, and the peak of 425 nm may be the result of the singly...

Ngày tải lên: 20/06/2014, 20:20

... presence of two absorption bands at 1273.7 and 3421 cm−1 belonging to the C=S and NH groups, respectively, and by absence of SH Density functional theory (DFT) studies DFT calculations of the IMHC ... nature of functional group of IMHC The appearance of a broad strong band in the IR spectra of IMHC in 3421 cm–1 is assigned to N–H stretching vibrations of the primary amine group The spectrum of ... synthesis of some bioactive compounds such as ß-lactams [16–18], and employed as ligands for the complexation of metal ions [19] SBs and their complexes are largely studied because they interested and...

Ngày tải lên: 20/06/2014, 20:20

... the design of the study and performed the electrical studies SK and MA carried out the structural studies SAA and MSA performed the thermal studies MAMK and MA also involved in writing of the manuscript ... observed The copper and the carbon peaks correspond to the carbon coated copper grid of TEM No peaks of other impurity have been detected Therefore, the EDX profile of sample (inset of Figure 2) indicates ... http://www.nanoscalereslett.com/content/6/1/434 Page of A B Figure FETEM and HRTEM images of silver nanoparticles (a) FETEM image of silver nanoparticles (b) High-resolution image of a single silver nanoparticle and inset shows...

Ngày tải lên: 21/06/2014, 02:20

Ngày tải lên: 21/06/2014, 02:20

... reports as well [31] The XPS data of Zn2p and Mn2p further provide evidences for the incorporation of Mn2+ ions into ZnO To obtain a better understanding of the formation of Mn-doped ZnO HMSs, a probable ... coordination route of EG which directs the formation of Mn-doped ZnO HMSs in view of morphology transformation A lot of EG molecules are adsorbed on the (001) surface of the ZnO crystals and block the ... spectra The origin of the peaks at 425 and 475 nm could be ascribed due to the transition occurring from Zn interstitials to the valence band, and the peak of 425 nm may be the result of the singly...

Ngày tải lên: 21/06/2014, 17:20

... method of functionalizing QDs is simple and can avoid complicated synthesis and characterization of the intermediate products of QDs, when reactions are performed on the QD surfaces A number of reports ... nm) determined from the correlation of particle size and optical band gap Further, average size and size distribution of CdTe QDs obtained from UV spectra and TEM image were also supported by ... binding constants KSV and Kb and binding stoichiometry of the complex (n) have been determined Significant differences on the values of binding constants for dendrimers of different generation...

Ngày tải lên: 21/06/2014, 20:20

... oxygen atom of the ketone (cyclohexanone) and the proton of the hydroxyl group of the alkanol – R – O… H – O – Volumetric and Thermodynamic Studies 106 Furthermore, the addition of N, N-dimethylformamide ... were evidently due to the rupture of the hydrogen bonds in pure cyclohexanone and 1-alknaols and DMF-DMF, dipole-dipole interactions, and the formation of O–H…C=O and perhaps even O–H ….N(CH3)2 ... concentrations, and this has been interpreted to arise from the presence of strong or weak interactions The extent of deviation depends upon the nature of the constituents and composition of the mixtures...

Ngày tải lên: 07/08/2014, 14:20

... lupus nephritis from randomised trials and observational studies Materials and methods QUOROM (quality of reporting of meta-analyses) and MOOSE (meta-analysis of observational studies in epidemiology) ... maximum of five points were awarded to studies according to whether they were randomised, double-blind, and accounted for withdrawals or drop-outs and to whether the methods of randomisation and ... trial) and over a treatment duration of up to years Despite this, there is the possibility of residual bias in observational studies and lack of blinding in randomised trials The amount of information...

Ngày tải lên: 09/08/2014, 08:23