pacemaker activity of the sa node insights from dynamic clamp experiments

... NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out of the equatorial plane (Table 3) The angles around the metal cofactor ... on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors Finally, by comparing ApeSOD with the SOD from the thermophilic ... [26] These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 suppresses the release of the peroxide product This may be one of the reasons...

Ngày tải lên: 14/02/2014, 22:20

... procedures The enzyme activity is expressed as percentage of the maximum activity recorded at pH in the absence of NaCl Values are the mean of three assays and the percentage standard error of the mean ... at the S2¢ pocket The replacement of Val372 of ACER with the polar Thr364 in ANCE probably contributes towards the lack of inhibitory activity of RXPA380 This supports the hypothesis that the ... deviations of the parameters calculated from the fitted curve by figp The Ki of inhibition of ANCE by [Thr6]BK was determined by measuring the kinetics of Abz-YRK(Dnp)P hydrolysis in the presence of 0,...

Ngày tải lên: 23/03/2014, 11:20

... [15,16] and of the Mtg from the same organism [17], the GTase module of PBP1a from Aquifex aeolicus [18,19], and the full-length PBP1b from Escherichia coli [20] For a long time, the functional ... characterization of the GTase activity of class A PBPs or Mtgs has been limited to basic studies by the lack of availability of the lipid II substrate and analogs [21] The development of chemically synthesized ... pentapeptide disaccharide is lower than that of the dansylated lipid II, the GTase activity can be followed as a decrease of dansyl fluorescence Our multiwell version of the assay is initiated by the addition...

Ngày tải lên: 29/03/2014, 21:20

... there are no reports about the chemical content and biological effect of the essential oils of L resedifolia There no reports on the essential oils of the species of the genus launaea except that ... noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this endemic species 2.6 Antimicrobial activity The quantification of antibacterial ... continuation of our phytochemical and antibacterial studies of the Algerian Sahara medicinal plants [7–11], we report here the findings of our studies on the composition and antimicrobial activity of L...

Ngày tải lên: 20/06/2014, 20:20

... there are no reports about the chemical content and biological effect of the essential oils of L resedifolia There no reports on the essential oils of the species of the genus launaea except that ... noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this endemic species 2.6 Antimicrobial activity The quantification of antibacterial ... continuation of our phytochemical and antibacterial studies of the Algerian Sahara medicinal plants [7–11], we report here the findings of our studies on the composition and antimicrobial activity of L...

Ngày tải lên: 21/06/2014, 17:20

... reported in the literature [12] and stored on the MS library (NIST database) The concentration of the identified compounds was computed from the GC peak total area without any correction factor The compounds ... noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this endemic species Chahma A voucher specimen was deposited at the herbarium ... Esters Aldehydes Page of demonstrated by the size of the microorganism growth inhibition zone around the filter paper disk, which is typically expressed as the diameter of the inhibition zone in...

Ngày tải lên: 21/06/2014, 19:20

... A significant step forward is the synthesis of available research on updating of CPGs included in the handbook of the Programme of Clinical Practice Guidelines in the Spanish National Health System ... of relevant comments about decision-making process of the need of updating able to share the burden of the development process There is a perceived need for international collaboration, but the ... partially funded within the framework of collaboration of the Quality Plan for the Spanish National Health System, under the terms of the collaboration agreement signed by the Carlos III Health...

Ngày tải lên: 10/08/2014, 11:20

... found in the GNA/Man complex (RCSB PDB code 1MSA) [17] was calculated using the forcefield of Discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin The ... (code 1MSA) [17] were taken from the RCSB Protein Data Bank [18] and used to build the three-dimensional model of the GNA-like lectin from maize The amino acid sequence alignment Page of 16 was ... correction of the geometry of the loops using the minimize option of TurboFrodo, a final energy minimization step was performed by 100 cycles of steepest descent using Discover 3, keeping the amino...

Ngày tải lên: 13/08/2014, 01:20

... anticancer activity However, it is still unclear whether the anticancer activity of the PR domain is due to its methyltransferase activity or the interaction 166 with the PRB motif Further studies ... suppressor activity The PR domain of RIZ1 possessed anticancer activity from the transfection studies This finding leaves us an important question on the roles the other structural components of RIZ1 ... supported from a research grant from the Cancer Research Society of Canada W Sun would also like to acknowledge the College of Pharmacy and Nutrition, University of Saskatchewan for the awarding of...

Ngày tải lên: 25/10/2012, 11:15

... by taking the square roots of ˆ the diagonal elements of the covariance matrix Σn GLS The values of the weights involved in the GLS estimation depend on the values of the fitted model These values ... of R(t) The uncertainty in the resulting estimate of R(t) can be assessed by repeated sampling of parameter vectors from the corresponding sampling distribution obtained from the asymptotic theory, ... reported by the CDC in the US during the 1999–00 season [12] The number of H3N2 cases (isolates) is displayed as a function of time Time is measured as the number of weeks since the start of the year’s...

Ngày tải lên: 13/02/2014, 16:20

... F is the normalized uorescence, [P] is the concentration of TNP-ATP, [E] is the concentration of the enzyme, c is the enhancement of uorescence intensity of the bound probe relative to the free ... docked to a surface around N398 at the rear site of the N-domain (pNPP site) of the same model (C) Docking of pNPP to the ATP binding site of the model containing the N-domain only, modeled according ... N-domain is due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding pocket is achieved by W411 and L414 The phosphate group...

Ngày tải lên: 19/02/2014, 16:20

... their N-oligosaccharides strongly affected their GABAuptake activity These indicate that the terminal structure of the oligosaccharides facilitate efficient GABA-uptake activity of the GABA transporter ... on the plasma membrane The activity of GABA-uptake by NNN was set at 100% The values represent the mean ± SEM of five separate experiments is similar to that of NNN containing N-glycans of the ... measurement of the activity of GABAuptake, an aliquot of the stable CHO transfectants was used for GABA-uptake assay, and another aliquot was used to determine the quantity of plasma membrane proteins of...

Ngày tải lên: 19/02/2014, 17:20

... (Table 1) The binding of the ®rst CN± ligand to the iron was thus more easy in the hydrophobic binding pocket of the antibody than the binding of the second one, most probably because of the steric ... and, in the more favorable conformation, H2O2 binds to the iron on the same face of the porphyrin as the catalytic COOH residue, the imidazole binding to the iron on the opposite face of the porphyrin ... two-thirds of the porphyrin moiety was inserted in the antibody pocket, three of the ortho-carboxylate substituents of the meso-phenyl rings being recognized by the side chains of amino acids of the...

Ngày tải lên: 21/02/2014, 03:20

... processes We then addressed the question as to whether high SUT2 gene dosage suppresses the slow growth phenotype of Dgpa2 Dras2 cells by increasing PKA activity Low activity of the PKA pathway ... final D600 of (± 0.5) A fraction of these cultures (equaling approx 50 mg of wet weight cells) was collected to determine the level of storage carbohydrates after entry of stationary phase The remainder ... [24] which in the wild type requires high PKA activity These consequences of PKA-pathway activity were tested subsequently in order to examine the influence of high copy SUT2 on PKA activity We...

Ngày tải lên: 07/03/2014, 15:20

... atoms of Ala19 In addition, the C-terminus folds back onto the core of the protein thereby allowing the side chain of Leu86 to partly fill the hole created by the removal of Ile14 Due to these ... used for the calculations Therefore, we recalculated the structure of the wild-type protein on the basis of the restraints used previously [15] with the same protocol used here for the Incidence ... computed as the ratio of the standard deviations of the chemical shifts of the amide nitrogen and proton nuclei Results Determination of the three-dimensional structure of HPr(I14A) To allow the comparison...

Ngày tải lên: 07/03/2014, 16:20

... 3.75) The decrease in the amount of [c-32P]GTP was determined by the increase in the amount of the 32Pi The same procedure was used for the ATPase assay In the second method [16], GTPase activity ... final volume of 20 lL of TMD buffer The reaction was initiated by the addition of ATP or GTP and continued for 10 at room temperature Then, lL of 10· SDS sample buffer was added One lL of the reaction ... detection of Ndk in the culture supernatant of M tuberculosis H37Rv is caused by the secretion rather than by the autolysis of the cells, culture supernatant was also analysed for the presence of a...

Ngày tải lên: 08/03/2014, 08:20

... CD If the sum of the individual CD curves of the two interacting partners is different from the CD of the ligand– receptor mixture, it is a clear indication of not only binding, but also of a ... followed by the spectra of the antibacterial peptide–protein fragment mixtures While the measured spectrum of the E coli fragment–pyrrhocoricin mixture is different from the mathematical sum of the individual ... no influence on the gross antibacterial activity [28] The presence of the carbohydrate side-chain, however, may modify either the binding to the target protein or the ability of the peptide to...

Ngày tải lên: 08/03/2014, 10:20

... (Glu429) The interactions of Zn1 and the carboxyl oxygen of Asp332 (Asp427) are weak The peptide is further stabilized at the active site by the interaction of the N-terminal amine of the P1 residue ... oxygen of Asp273 (Asp366), the carbonyl oxygen of the P1 residue with the side-chain amine of Lys262 (Lys354), and the backbone amino group of the P1¢ residue with the backbone carbonyl oxygen of ... with the role of Lys282 in PepA (blLAP Lys262) in binding of the carboxyl oxygen of the P1 residue and stabilization of the gem-diolate transition state The Ala substitution mutant (K354A) of the...

Ngày tải lên: 08/03/2014, 23:20

... binding of T), at a pH higher than when it was isolated The T domain facilitated the insertion of the C domain in the membrane To better characterize the environment of the Trp residues of the C ... greatly displace the native to MG state equilibrium of the C domain in favor of the MG state The T domain favors the interaction of the C domain with the membrane, because it brings the C domain ... function of pH In the case of T and CTW50 ⁄ 153F, the data could be fitted with the pK1 ⁄ values obtained from (A) and (B) in order to estimate the kmax of the various states of the domain (continuous...

Ngày tải lên: 14/03/2014, 22:20

... 2) The fusion protein was successfully expressed in the Pichia strain X-33 Because of the presence of the a-mating sequence from Saccharomyces cerevisiae at the N-terminus of the construct, the ... perform the superimposition of the models procheck [46] was used to check the stereochemical quality of the three-dimensional model: 82.8% of the residues were assigned to the favorable regions of the ... with the carbohydratebinding properties of the lectin (results not shown) Agglutination activity and carbohydrate-binding properties of recombinant Orysata To study the biological activity of the...

Ngày tải lên: 14/03/2014, 23:20