p44 42 map kinase assays
Báo cáo Y học: CTGF/Hcs24 induces chondrocyte differentiation through a p38 mitogen-activated protein kinase (p38MAPK), and proliferation through a p44/42 MAPK/extracellular-signal regulated kinase (ERK) doc
... protein (MAP) kinases are involved in interleukin-1 (IL-1)-induced IL-6 synthesis in osteoblasts: modulation not of p38 MAP kinase, but of p42 /p44 MAP kinase by IL-1-activated protein kinase C ... mediated by the following kinases; p44/ 42 MAPK/ERK, p38 MAPK, and c-jun N-terminal kinase (JNK) It is reported that Elk-1 and Atf-2 are phosphorylated by ERK and p38 MAPK, respectively [30,33,34] ... phosphorylation of ERK and p38 MAP kinase in HCS-2/8 cells We analyzed the effects of rCTGF/Hcs24 on the phosphorylation of two major types of MAP kinases, ERK and p38 MAP kinase, by Western blotting...
Ngày tải lên: 24/03/2014, 04:21
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... CTGF/CCN2 gene and that signaling mechanisms via p38 MAP kinase were involved as well The p38 MAP kinase seemed to act as a downstream effector of Cdc42, but not RhoA or Rac1, even though all three ... Gaestel, M., Resch, K & Holtmann, H (1999) The p38 MAP kinase pathway signals for cytokine-induced mRNA stabilization via MAP kinase- activated protein kinase and an AU-rich region-targeted mechanism ... GTPase and MAP kinase signaling pathways in the modulation of the CTGF/CCN2 gene in response to diverse extracellular stimuli known for their ability to activate the Rho GTPase and/or MAP kinase...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt
... mitogen activating protein (MAP) kinase (p38 kinase) followed by caspase-3 activation [24] Mitogen-activated protein kinases (MAPKs) are a group of protein serine ⁄ threonine kinases that play central ... lineages MAPK may either contribute to or prevent cell death, depending on the duration of activation and the balance of activity between the MAP kinase, ERK, and the stress-activated kinases, ... [15,23] In the current study, we planned to determine whether either of the other two MAP kinases, ERK and p38 kinase, and activation of their target transcription factors, such as ATF-2, could...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
... three-tiered MAPK modules, each of which consists of a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase (MAPKKK) MAPK modules operate in numerous biological settings where, through ... signalregulated kinases (ERKs), the Jun N-terminal kinases (JNKs) and the p38 MAP kinases, among others These enzymes are terminal components of three-tiered MAPK modules, each of which consists of a MAP kinase ... phosphorylated on Tyr180 and Thr182 by the upstream activators MAP kinase kinase (MKK3) and MAP kinase kinase (MKK6) Once activated, p38 MAPKa exerts its effect by directly phosphorylating transcription...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling Delivered on 24 October 2002 at the 28th FEBS Meeting in Istanbul potx
... (1993) Growth Factors Induce Nuclear Translocation of MAP Kinases (p42maPk and p44mapk) but not of Their Activator MAP Kinase Kinase (p45mapkk) in Fibroblasts J Cell Biol 122, 1079–1089 54 Robinson, ... contains a mitogen-activated protein kinase targeting motif Mol Cell Biol 18, 710–720 Fukuda, M., Gotoh, Y & Nishida, E (1997) Interaction of MAP kinase with MAP kinase kinase: its possible role in the ... mitogen-activated protein kinase signalling Curr Opin Cell Biol 12, 186–192 48 Brondello, J.M., Pouyssegur, J & McKenzie, F.R (1999) Reduced MAP kinase phosphatase-1 degradation after p42/ p44MAPK-dependent...
Ngày tải lên: 31/03/2014, 07:20
map kinase signaling protocols
... (1993) Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts J Cell Biol 122, 1079–1088 123 Adachi, ... Ser/Thr kinases at the MAPKAPK level This group of protein kinases includes the ribosomal S6 kinase (RSK) (26), the MAPK/SAPK-activated kinase (MSK) (27), and MAPK signal-interacting kinase (MNK1) ... following tiers, which are referred to as MAPK kinase (MAPKK), MAPK, and MAPK-activated protein kinase (MAPKAPK) The four to five tiers in each of the MAPK cascades are probably essential for...
Ngày tải lên: 11/04/2014, 09:50
báo cáo hóa học:" Luteinizing hormone-induced Akt phosphorylation and androgen production are modulated by MAP Kinase in bovine theca cells" doc
... protein kinase A; CYP17A1: 17α-hydroxylase/C17-20 lyase cytochrome P450; StAR: steroidogenic acute regulatory protein; ERK: extracellular-signal regulated kinase; MAPK: mitogen activated protein kinase; ... Nevertheless, LH reportedly activates the extracellular-signal-regulated kinases (ERK)/mitogen activated protein kinase (MAPK) pathway in ovarian granulosa and theca cells [12] Although FSH and ... protein kinase; PI3K: phosphatidyl inositol 3 -kinase; EIA: enzyme immunoassay; RT-PCR: reverse transcription polymerase chain reaction; MEK: MAPK/ERK kinase; 36B4: acidic ribosomal phosphoprotein;...
Ngày tải lên: 20/06/2014, 07:20
Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-B pathway doc
... min) for various periods of time To determine the roles of c-Jun N-terminal kinase (JNK), p38 MAP kinase or p42 /p44 MAP kinase in the expression of stretch-induced TLR4 expression, cardiomyocytes ... SB203580 is a highly specific, cell permeable inhibitor of p38 kinase PD98059 is a specific and potent inhibitor of p42 /p44 MAP kinase For evaluation of secreted TNF-a on TLR4 expression, conditioned ... receptor antibody (a neutralizing antibody, R&D Systems), polyclonal anti-p38 MAP kinase and monoclonal anti-phospho p38 MAP kinase antibodies (Cell Signaling, Beverly, MA, USA), anti-mouse monoclonal...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo y học: " Regulation of IκBα expression involves both NF-κB and the MAP kinase signaling pathways." ppt
... the effect of bortezomib and several MAP kinase inhibitors on LPS-induced IκBα expression The results that follow suggest that, in addition to NF-κB, the MAP kinase signaling pathway is involved ... increase of luciferase activity in the liver Effect of the MAP kinase inhibitors on IκBα induction by LPS We examined the effect of MAP kinase inhibitors SB203580, PD098059 and SP600125 on LPS-induced ... since treatment PD098059 + LPS SP600125 + LPS Figure Effect of MAP kinase inhibitors on LPS-induced luciferase expression Effect of MAP kinase inhibitors on LPS-induced luciferase expression A Female...
Ngày tải lên: 11/08/2014, 08:21
Báo cáo y học: "P38 MAP kinase inhibitors as potential therapeutics for the treatment of joint degeneration and pain associated with osteoarthritis" ppsx
... MAPK pathway [17] The MAPKs operate as a series of kinase modules beginning with the MAPK kinase kinases (MKKKs), which phosphorylate MAPK kinases (MKKs), which ultimately phosphorylate MAP kinases ... which ultimately phosphorylate MAP kinases (MAPK) [1] There are MAPK families, the extracellular-regulated protein kinases (ERK), the c-Jun NH2-terminal kinase (JNK) and p38 http://www.journal-inflammation.com/content/5/1/22 ... versus large panels of kinases [23,24] SB-203580 has good selectivity for p38α and β over the majority of kinases in a panel of over 300 kinases but does inhibit some MAPKs such as JNK with an...
Ngày tải lên: 11/08/2014, 08:22
Báo cáo y học: "Inhibitory effect of IL-8 on insulin action in human adipocytes via MAP kinase pathway." doc
... in human aortic endothelial cells cultured with high glucose by inhibiting the p44/ 42 mitogen activated protein kinase Br J Pharmacol 2001, 134:753-762 Temaru R, Urakaze M, Satou A, Yamazaki ... in brown adipocytes of Wistar rats by impairing TNF-alpha induction of p38 and p42 /p44 mitogen-activated protein kinases Diabetologia 2004, 47:1615-2447 Publish with Bio Med Central and every ... Those data suggest that IL-8 inhibits the insulin signal pathway via the ERK pathway and p38 MAP kinase pathway Discussion In the present study, we demonstrate for the first time that IL-8 per...
Ngày tải lên: 11/08/2014, 08:22
Map kinase phosphatase 2 regulates macrophage adipocyte interaction
... lacking MAP kinase phosphatase1 have enhanced MAP kinase activity and resistance to diet-induced obesity Cell Metab 4: 61–73 PMID: 16814733 14 Wu Z, Jiao P, Huang X, Feng B, Feng Y, et al (2010) MAPK ... [7,9] Mitogen-activated protein kinase (MAPK) phosphatases (MKPs) or dual specificity phosphatases (DUSPs) are major negative regulators of MAPKs [10] They inactivate MAPKs through dephosphorylation ... innate and adaptive immune responses by MAP kinase phosphatase Nature 430: 793–797 PMID: 15306813 31 Zhao Q, Wang X, Nelin LD, Yao Y, Matta R, et al (2006) MAP kinase phosphatase controls innate immune...
Ngày tải lên: 22/09/2015, 15:19
Characterisation of plasmodium falciparum pfnek3, an atypical MAP kinase activator
... conserved MAPK activation motif (TXY) by a specific MAPK kinase (MAPKK; also called MEK, for MAPK/ERK kinase) is necessary for MAPK activation Correspondingly, another upstream kinase, MAPKKK or ... candidate kinases upstream of Pfmap1 and Pfmap2 For example, it has previously been suggested that Pfnek1, a NIMAͲ family kinase is an upstream regulator (i.e MAPKK, also called MEK, MAPK/ERK Kinase) ... LuriaͲBertani Mammalian two hybrid MitogenͲactivated protein kinase MAPK kinase MAPKK kinase Myelin basic protein Messenger ribonucleic acid NIMAͲlike kinase Never in mitosis, Aspergillus NͲterminal region...
Ngày tải lên: 03/10/2015, 11:42
Báo cáo khoa học: Investigation of leucine-rich repeat kinase 2 Enzymological properties and novel assays pptx
... mutations in leucine-rich repeat kinase augment kinase activity Proc Natl Acad Sci USA 102, 16 842 16847 Smith WW, Pei Z, Jiang H, Dawson VL, Dawson TM & Ross CA (2006) Kinase activity of mutant LRRK2 ... protein kinases [27], whose members have both serine ⁄ threonine and tyrosine kinase activity With respect to LRRKtide, we have demonstrated that LRRK2 acts purely as a serine ⁄ threonine kinase, ... autophosphorylation [22] Many protein kinases require phosphorylation of residues within their kinase domains to be activated [23], and therefore can act as substrates of kinases, including themselves...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo y học: " Theoretical basis for reducing time-lines to the determination of positive Mycobacterium tuberculosis cultures using thymidylate kinase (TMK) assays" ppt
... [9,14-16] Specifically, some of these modified culture assays such as phage amplifica- http://www.tbiomed.com/content/6/1/4 tion assays, line probe assays and colorimetric redox-indicator methods can ... catalyzed by enzymes called kinases or phosphotransferases [18] Among mammalian cells, two phosphotransferases exist: thymidine kinases (types and 2) and thymidylate kinase [18] Sherley and Kelly ... emerging TB assays are, however, still not readily available for routine use in most resource-limited settings where the TB burden is concurrently high [9] We hypothesized that metabolomic-based assays...
Ngày tải lên: 13/08/2014, 16:21
MIND MAP – BẢN ĐỒ TƯ DUY
... ông trình bày cách thức ghi nhớ tự nhiên não với phương thức Mind Map Ngoài ra, ông có số sách tiếng “Use your memory”, “Mind Map Book” GVHD : GS TSKH Hoàng Văn Kiếm | SVTH : Trần Minh Sang ... | SVTH : Trần Minh Sang Ki Tr T r a n g | 26 Phụ Lục D Sơ lược Tony Buzan Tony Buzan sinh năm 1 942, Londo Ông có nhiều nghiên cứu thuyết phục não Quyển sách “ Use your head” ông dịch nhiều thứ ... Nam) để giảng dạy “giải phóng” sức mạnh não người Tony Buzan người sáng tạo phương pháp tư Mind Map (Bản Đồ Tư Duy) Tony Buzan nhận danh dự tâm lý học, văn chương Anh, toán học nhiều môn khoa...
Ngày tải lên: 14/09/2012, 10:51
Test GMAT 42
... 25 26 30 10 49 43 48 24 25 30 48 42 48 23 25 29 47 42 47 22 24 28 46 41 46 21 23 27 45 40 45 20 22 27 44 39 44 19 22 26 43 39 44 18 21 25 42 38 43 17 20 24 41 37 42 16 20 24 40 37 41 15 19 23 39 ... 43 450 13 290 97 730 72 600 42 440 12 290 96 720 71 590 41 440 11 280 95 720 70 590 40 430 10 280 94 710 69 580 39 430 270 93 710 68 580 38 420 260 92 700 67 570 37 420 250 91 700 66 570 36 410 ... Graduate Management Admission Test (GMAT®) with test code 42 If the first two digits of the test code on your answer sheet (item on Side 1) are not 42, please contact ETS to send you the correct booklet...
Ngày tải lên: 02/10/2012, 14:10
Hình học xạ ảnh 42
... Bài 42: Chứng minh A, B hai điểm phân biệt hyperbol đường thẳng AB cắt hai đường tiệm cận hai điểm C,...
Ngày tải lên: 03/10/2012, 15:30
