organic chemicals an environmental perspective

... 4-chloroanilines and 4-fluoroaniline by a Moraxella sp strain G (Zeyer et al 1985), 3-, and 4-methylanilines by P putida mt-2 (McClure and Venables 1986), 2-methylaniline and 4-chloro-2methylaniline ... monooxygenase produces phenanthrene 3,4-oxide and phenanthrene 9,10oxide that are further transformed into phenanthrene trans-dihydrodiols and phenanthrols (Sutherland et al 1991) Ph chrysosporium ... (Gibson et al 1995), and the stereospecific hydroxylation of (R)-1-indanol and (S)-1-indanol to cis-indan-1,3-diol and trans (1S,3S)-indan-1,3diol (Lee et al 1997); the indantriols are also formed...

Ngày tải lên: 11/08/2014, 04:20

... thanks to my colleagues: Choo En, Liang Zhian, Dr Wu Xiaoyu, Dr Cheng Lili, Dr Xu Liwen, Dr Wang Youqing, Dr Yuan Qing, Han Xiao, Zhu Qiang, Luo Jie and other labmates for their cordiality and ... the other hand, an organocatalyst is an organic compound which exhibits catalytic activities The use of organocatalysts would not induce contamination, a disadvantage inherent in the transition ... moisture and oxygen, demanding reaction conditions are usually not required in many cases Furthermore, these organocatalysts can be anchored to a solid support and reused more conveniently than organometallic/bioorganic...

Ngày tải lên: 14/09/2015, 08:39

... Butaprost was from Cayman Chemical (Ann Arbor, MI, USA) Antibodies against PAR-1 (WEDE15) were from Immunotech, antibodies against AP-2 were from Abcam (Biozol, Eching, Germany), and antibodies against ... of two transcription factors, i.e Sp1 and AP-2 Sp1 acts as a positive regulator, and AP-2 as a negative regulator [18,19] Moreover, in cancer cell lines and cells isolated from malignant tissues, ... PAR-1, and analyzed by flow cytometry (B–E) Representative histograms obtained by flow cytometry analysis in unstimulated hLFs and cells treated with PGE2 (B), But (C), ISO and NECA (D), and FSK...

Ngày tải lên: 30/03/2014, 04:20

... transcription and with no template cDNA were performed with each assay To compensate for variations in input RNA amounts, and efficiency of reverse transcription, b-actin mRNA was quantified and ... the p65 antibody Endothelial cells were transfected with pNF-jB-SEAP in the presence of the pSEAP as a control vector The cells were preincubated with antioxidant NAC (10 mM) for 30 and then ... USA) and processed directly to cDNA synthesis using the TaqMan Reverse Transcription Reagents kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s protocol The PAI-1 and...

Ngày tải lên: 30/03/2014, 11:20

... The following antibodies were used: rabbit anti-Bax (Santa Cruz Biotechnology), mouse anti-Bcl-2 (Santa Cruz Biotechnology), and peroxidase-conjugated secondary antibody (Sigma) The bands were visualized ... Chlamydia trachomatis J Antimicrob Chemother 63, 115–23 (2009) 12 YE Zhang, JN Wang, JM Tang, LY Guo, JY Yang, YZ Huang, Y Tan, SZ Fu, X Kong, F Zheng, In Vivo Protein Transduction: Delivery of ... participated in animal experiments LYG made fusion protein and evaluated the apoptosis by Flow Cytometry YL and SYC analyzed the results and help writing the manuscript All the authors have read and approved...

Ngày tải lên: 18/06/2014, 19:20

... observed under a Leitz microscope Statistical analysis Data were analyzed by one-way analysis of variance (ANOVA) followed by Dunnett' test All analyses were performed using the Graph-Pad Prism® ... considered significant Authors' contributions D M coordinated and participated in the design of the study, statistical analysis and the drafting of the manuscript V P-M carried out and participated ... of CCR5 and high production of beta-chemokines Virology 1998, 244:66-73 Zhang L, He T, Talal A, Wang G, Frankel SS, Ho DD: In vivo distribution of the human immunodeficiency virus/simian immunodeficiency...

Ngày tải lên: 18/06/2014, 22:20

... ZO-1 were detected using anti-occludin, anti-claudin-5, and anti-ZO-1 mouse monoclonal antibodies (all purchased from Zymed, South San Francisco, CA) Blots were washed and incubated with horseradish ... of antibodies to IL-6 and GM-CSF on LPS-induced changes in the permeability of BMECs to 131I-HIV-1 (A and C) and TEER (B and D) LPS (100 µg/mL) was added to the luminal chamber In panels A and ... LPS and antibodies to IL-6 or GM-CSF in the 15 luminal chamber attenuated LPS-enhanced HIV-1 transport across the BMEC monolayer without a change in TEER (Fig 1A and 1B) BMECs secrete IL-6 and...

Ngày tải lên: 19/06/2014, 22:20

... observed under a Leitz microscope Statistical analysis Data were analyzed by one-way analysis of variance (ANOVA) followed by Dunnett' test All analyses were performed using the Graph-Pad Prism® ... considered significant Authors' contributions D M coordinated and participated in the design of the study, statistical analysis and the drafting of the manuscript V P-M carried out and participated ... of CCR5 and high production of beta-chemokines Virology 1998, 244:66-73 Zhang L, He T, Talal A, Wang G, Frankel SS, Ho DD: In vivo distribution of the human immunodeficiency virus/simian immunodeficiency...

Ngày tải lên: 20/06/2014, 04:20

... supported by a grant from the Korean Health 21 R&D Project, Korean Ministry of Health and Welfare (grant number 01-PJ3PG6-01GN11-0002) and by the Korean Science and Engineering Foundation (grant number ... various fields and in a variety of cell systems and include inhibition of apoptosis by transduction of anti-apoptotic Bcl-XL protein in explant cultured human chondrocytes and in human islet cells ... monolayer and explant cultures, and that the transduced fusion protein has a regulatory function for the IL-1 induced catabolic pathway in chondrocytes Materials and methods Reagents Recombinant human...

Ngày tải lên: 09/08/2014, 08:22

... Tram and Nguyen Van Ngai for follow-up of the exposed uninfected individuals, Annie David for technical assistance, Nathaniel Landau and Jay Levy for the gift of plasmids and vectors, and Ioannis ... neutralizing anti-RANTES (5 µg/ml), anti-MIP1α (15 µg/ml) and anti-MIP1β (25 µg/ml) (R&D systems, France) mAbs (black bars) or with an isotype control antibody (45 µg/ml) (patterned bars) The antibodies ... LC, WC, DSA and GP participated in the data analysis ASC and GP drafted the manuscript All the authors critically reviewed and approved the final manuscript 14 15 Acknowledgements We thank Luong...

Ngày tải lên: 13/08/2014, 09:20

... Spectroscopy Laboratory and other facility resources for their kind assistance and cooperation Many thanks are extended to following persons: Chen Min, Ren Feng, Sebastian Muthu, Janardhana Prabharathy, ... Pt and Pd Centers and Acetylene and Vinylidene iv 4.1 Introduction 103 4.2 Objectives 104 4.3 Results and Discussion 108 4.3.1 Syntheses and Structures 108 4.3.2 1H and 31P NMR of complexes , and ... Lin Ronger, Xu Huan, Zhang Ke , Hu Jian and other members in Dr Yip’s group for their help and cooperation I wish to acknowledge the National University of Singapore for the financial support Finally,...

Ngày tải lên: 17/09/2015, 17:17

... quan tâm Gia nhập: Jul 2010 Nơi cư ngụ: QuangZ hou Bài gửi: 1,752 Sửa sonvalentine : 04-06-2011 lúc 12:10 PM mrdang89, lionmonkey, Vnn_68 and 12 others like this Canon EOS 1Ds Mark III,Lens:Canon ... Nơi cư ngụ: QuangZ hou Bài gửi: 1,752 Ngày tập ngực đưa em đên tượng chai ngực nhanh Sửa sonvalentine : 04-06-2011 lúc 12:30 PM mranhbb, nhson219, giangnam233 and others like this Canon EOS 1Ds ... sonvalentine : 04-06-2011 lúc 08:59 AM hemattroi, y06a_ktanh, whitegun and 34 others like this Canon EOS 1Ds Mark III,Lens:Canon EF 70- 200mm/2.8L IS II, Canon EF 85mm Honda CBR 600cc 2008 Tôi Đi tim PDFmyURL.com...

Ngày tải lên: 27/08/2012, 09:21

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Ngày tải lên: 09/10/2013, 12:20

... Pioglitazone, PGJ2 and Ac-DEVD-CHO were from Cayman Chemical Company (Ann Arbor, MI, USA) Antibodies against Akt-1, phosphorylated Akt-1 (pAkt-1) (Ser473), Bcl2, Bax and Hsp90 were from Santa Cruz Biotechnology, ... xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis Apoptosis 12, 1953–1963 15 Yang JY, Della-Fera MA & Baile CA (2008) Guggulsterone inhibits adipocyte differentiation and ... adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662, a PPARc antagonist Further analysis...

Ngày tải lên: 16/02/2014, 09:20

... a-, b- and c subunits, whereas only the a subunit is bound to GDP GPCR are transmembrane receptor proteins, containing seven membrane-spanning segments After binding of the relevant ligand and activation ... 5¢-CC ACTTGGTTTGCTACGA-3¢ (antisense) Acknowledgements We appreciate the excellent technical assistance of Suhad Al-Badri and Franziska Daduna We thank Roland Lang and Jorg Mages (Technical University ... expression can be increased by LP and LPS Thus, these results demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modulate expression after stimulation with LP and LPS Expression...

Ngày tải lên: 18/02/2014, 13:20

... cell and nuclear lysates with the C-YES polyclonal antibody and protein G sepharose, and the depleted supernatants and precipitates were western blotted with the C-YES antibody The supernatants and ... hand lane) or antibody and protein G but no lysate (far right hand lane) *Background caused by cross-reactivity with the depleting antibody (B) The C-YES depleted and mock depleted supernatants ... depleted with 20 lg of anti-C-YES (Upstate), lg of anti-WEE1 H-300 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or lg of anti-MYT1 N-17 (Santa Cruz Biotechnology) polyclonal sera and 15 lL (bed volume)...

Ngày tải lên: 18/02/2014, 18:20

... attenuates the febrile response and the levels of IL-1b, IL-6 and ROS in the supernatant fluids Adding an anti-IL-1b or anti-IL-6 monoclonal IgG to the supernatant fluids significantly decreases the febrile ... (3-[1-(p-chlorobenzyl)-5-(isopropyl)-3-t-butylthioindol-2-yl]-2,2-dimethylpropanoic acid; MK 886) (Table 2, Fig 3A, lanes and 4, and Fig 3B, lanes and 5) Wort, HIMO, ETYA, or MK 886 alone has no effect on the nuclear translocation or DNA-binding ... supernatant fluids obtained from SEC1-treated human PBMC in rabbits is associated with the levels of IL-1b, IL-6 and ROS in the supernatant fluids of SEC1-treated human PBMC Adding Fig Membrane translocation...

Ngày tải lên: 19/02/2014, 00:20

... lg) to wortmannin-inactivated aggregates was also tested (lanes 6–9) The aggregate and the supernatant (sup.) were analyzed separately (lanes 10 and 11) The conditions in lanes 4, and are parallel ... no DNA (lane 5) or aberrant types of DNA (1.0 lg), including relaxed closed circular (lane 6), NC, nicked form (lane 8) and SSC, single-strand circular (lane 9) derived from uX174, and linear ... aggregative organization composed of human nucleoproteins and aberrant DNA is structurally flexible and biochemically active, suggesting that the coacervate-like phase transition from random diffusion...

Ngày tải lên: 19/02/2014, 06:20

... inhibitors ATK and 4-bromophenacyl bromide and advice on the manuscript We are thankful to F.J Bruggeman for stimulating discussions and advice on the manuscript We also thank K Krab and H Dekker ... stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... in human tumors Oncogene 18, 813–822 21 Bos, J.L (1989) ras oncogenes in human cancer: a review Cancer Res 49, 4682–4689 22 Kholodenko, B.N (2000) Negative feedback and ultrasensitivity can bring...

Ngày tải lên: 19/02/2014, 16:20