observed variability should be explained via minimum number of anomalies
Báo cáo y học: "Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle" doc
... possibility of functional links between the above described alterations of gene expression and potential defects in signal transduction Analysis with protein microarrays of the status of a number of known ... stimulation Graphical representation of numbers of probesets showing differential gene expression in pair-wise SAM comparisons between the microarray hybridization data of WT fibroblasts that were serum-starved ... contrast to the profile of IE gene expression in H-ras-/- cells during G0/G1 transition, the profile of H-ras-/- cells stimulated with serum for hours showed a clear increase in the number of differentially...
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báo cáo khoa học: "Transcript profiling reveals expression differences in wild-type and glabrous soybean lines" pdf
... 27 Bernard RL, Singh BB: Inheritance of pubescence type in soybeans: glabrous, curly, dense, sparse and puberulent Crop Sci 1969, 9:192-197 28 Singh BB, Hadley HH, Bernard RL: Morphology of pubescence ... (Figure 4) The presence of two bands in CS root tissue might be explained by cross hybridization of the probe to more than one of the seven BURP genes present in the soybean genome as the BURP ... revealed a number of differentially expressed genes One highly-expressed gene, Glyma04g35130, in wild-type soybean was of interest as it has high homology to the cotton gene GhRDL1 gene that has been...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... Periodic box of TIP3P waters and 14 Na+ counter ions to neutralize the charge on the DNA strands of the hybrids are added using LEaP module of amber This results in 4294 and 4379 number of water ... out as described for PDwt and PDmut Using LEaP module of amber 6, 28 Na+ net neutralizing counter ions, and periodic box of TIP3P waters are added to each system A total number of 3985 and 4239 ... the fluctuations Complete loss of stacking interaction is seldom seen (A) of the amino group of C7 with A6 (Fig 4B) or through the overlap of the six-member ring of C7 with A6 (Fig 4C) Thus, unlike...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J subunits ... II (PSII) of C reinhardtii [16] We now report the expression of His- and Strep-tagged plant PSI Results A series of cassettes containing tagged variants of the full-length precursor of Arabidopsis ... predictions made by Ben-Shem et al [3] in the interpretation of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... binding of the ADP moiety of FAD) [11] and for the 70 residues at their C-termini (for this latter region a 31–40% of identity and a 53–61% of sequence similarity has been calculated) The results of ... amino acids present in the active site of MSOX [12] and of DAAO [15,16] (C) Comparison of the active site of the 3D-structure model of GO with the active site of MSOX from Bacillus subtilis (right) ... EcoRI site of the multiple cloning site of the expression vector pT7.7 downstream of the T7 RNA polymerase promoter to produce the recombinant plasmid pT7-GO Because of the presence of an ATG...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
... [14,15] Indeed, the mole number of ATP needed for one mole of lipid A in the core EFMs varies between 36 and 42 In contrast, the amount of NAD(P)H is 54 per mole of lipid A in all of these EFMs In this ... each function by the gene name augmented by a number The numbers are given by us and are not part of the of cial gene name Table gives the abbreviations of metabolites used in this study It is interesting ... the relation of this to the viability of the organism No coherent picture can be drawn at first glance For instance, suppressing the production of both forms of lipid A is predicted to be lethal...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... error of the mean Page of (page number not for citation purposes) Virology Journal 2007, 4:94 steps of virus maturation, as numerous electron dense particles, similar to those already described ... h before infection, using 125 ng/ml of phorbol myristate acetate and 50 ng/ml of ionomycine To determine cell viability, propidium iodide (BD Biosciences Pharmingen) was added at µg/ml just before ... T1 at a m.o.i of Five, 8, 12 and 24 hours following infection, cells were fixed and processed for electron microscopy as previously described [15] We observed a lot of virions adsorbed on the cell...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... error of the mean Page of (page number not for citation purposes) Virology Journal 2007, 4:94 steps of virus maturation, as numerous electron dense particles, similar to those already described ... h before infection, using 125 ng/ml of phorbol myristate acetate and 50 ng/ml of ionomycine To determine cell viability, propidium iodide (BD Biosciences Pharmingen) was added at µg/ml just before ... T1 at a m.o.i of Five, 8, 12 and 24 hours following infection, cells were fixed and processed for electron microscopy as previously described [15] We observed a lot of virions adsorbed on the cell...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
... in the text B) Number of genes (probe set) up or down regulated in npq1lut2 mutant compared to control wild-type plants at the three time points of the experiment (0, and 24 hours of treatment ... the 18 genes may be directly influenced by the lack of NPQ1 and LUT2 enzyme activities, or of the corresponding products (Figure 1) Following exposure to intense light, the number of differentially ... transcript levels between the two genotypes at the three time points, i.e 20 genes for time 0, 190 genes for time h and 414 genes for time 24 h (Figure 1) The minimum number of probe-sets per cluster...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... abscissionless leaf variety of pubescent birch has also been described [34] Peas are one of the world's most important grain legumes and serve as a valuable protein source in the diet of humans and animals ... abscission event was also observed to start at the centre of the seed coat/palisade junction, particularly where the counterpalisade layer becomes restricted Page of (page number not for citation ... ratio of LR white resin to ethanol At the end of the infiltration process, the specimens were transferred to an embedding mould and polymerised at 50°C for 24 h Plant materials Page of (page number...
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Tài liệu Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays doc
... forearm of each subject via the anticubital vein after a minimum of 12 hours of fasting Samples were collected in EDTA-treated and plain tubes The methods for determining the concentrations of each ... secretion of sCD40L may be one explanation for the increased inflammation associated with aging, and may be a pathway that links aging with an increased risk of atherothrombosis TGF-a, a member of the ... under the age of 45 and group consisted of 55 subjects over the age of 65 The study subjects were not permitted to perform strenuous exercise or drink alcoholic beverages 24 h before the laboratory...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... as a function of the elution time Numbers indicate the amount of 2-amino-2-N-acetylamino-D-glucose (GlcNAc) units in an oligomer; b and a indicate their isoform the elution profile of the selected ... thank Prof Steve C Fry, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, Ruhr University of Bochum, Germany, for critical reading of the ... with chain 3381 Enzymatic properties of chitinase A from Vibrio carchariae length of chitooligomers implies that the binding site must be composed of an array of subsites, probably six GlcNAc subsites...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... profile of the H334G mutant in the phosphorolysis direction was a narrow, bell-shaped curve, strikingly different from that of the wild-type and with an optimum pH of 6.5 A shift of the pH profile of ... and with the number of recorded scans and resulting signal-to-noise ratios indicated (B) Chemical 31P shifts of the PLP phosphate resonance signal of wild-type enzyme in the absence of ligand (d), ... relative elevation of pKa of PLP phosphate in unliganded CcStP and the apparent lack of perturbation of pKa in the CcStP complex with arsenate The pH dependence of the 31P chemical shift of PLP phosphate...
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Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc
... wild-type and mutant enzymes are numbered according to the sequence of the wild-type E coli enzyme starting with M1 as position Cyclization of Gln-OH The conversion of Gln-OH to 2-pyrrolidone-5-carboxylic ... NH2OH has been shown to be a substrate for E coli [46] and Lactococcus lactis [47] CTP synthases With the exception of Liebermans work in 1956 [46], little is known about the ability of E coli ... hydrolysis of GlnOH contains terms that include rate constants for the hydrolysis reaction, production of NH2OH and Glu, and release of Glu (The exact kinetic mechanism [i.e order of addition of substrates]...
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Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt
... can be explained by a change in polarity of some, or all of the four tryptophans in the active site tunnel of Cel7A, when cellotetraose is bound and can thus be used to monitor the binding of ... were observed in the mutant, apart from the changed atoms of the mutated side chains In order to evaluate further the design of the mutations, it was of interest how the stability behaviour of ... seems to be folded at this pH at 25 °C (Figs and 2), confirmed also with the CD measurements described below (Fig 6) The effect of a soluble substrate (cellotetraose) on the stability of Cel7A...
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Báo cáo sinh học: "Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays" pptx
... forearm of each subject via the anticubital vein after a minimum of 12 hours of fasting Samples were collected in EDTA-treated and plain tubes The methods for determining the concentrations of each ... secretion of sCD40L may be one explanation for the increased inflammation associated with aging, and may be a pathway that links aging with an increased risk of atherothrombosis TGF-a, a member of the ... under the age of 45 and group consisted of 55 subjects over the age of 65 The study subjects were not permitted to perform strenuous exercise or drink alcoholic beverages 24 h before the laboratory...
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o cáo hóa học:" Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays" ppt
... forearm of each subject via the anticubital vein after a minimum of 12 hours of fasting Samples were collected in EDTA-treated and plain tubes The methods for determining the concentrations of each ... secretion of sCD40L may be one explanation for the increased inflammation associated with aging, and may be a pathway that links aging with an increased risk of atherothrombosis TGF-a, a member of the ... under the age of 45 and group consisted of 55 subjects over the age of 65 The study subjects were not permitted to perform strenuous exercise or drink alcoholic beverages 24 h before the laboratory...
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Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans." potx
... of how isoforms change across development, although the identification of 5% of genes whose isoform patterns change across development is highly likely to be an underestimate Identification of ... indirect consequence of a loss of NMD In this case, rather than the pattern of splicing being identical in wild-type and mutant and differences in isoform levels being caused by a loss of NMD in the ... mutant animals? We believe that this may be the case and that this may be the result of one of two possible effects One alternative is that the level of splice factors is perturbed in animals defective...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty field samples from dogs, foxes, ... availability of genomic sequences, molecular diagnostic methods for detection of viruses have significantly improved[15] Complementary DNA and RNA probes have been used to detect RNA and mRNA of the ... repeatability of the multiplex RT-nPCR Serial 10-fold dilutions of CDV vaccine strain were subjected to amplification by multiplex RT-nPCR The lowest limit of detection with this method was shown to be...
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Báo cáo y học: "Comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus" doc
... analyses have not been performed Indeed, a portion of resistance attributes are likely to be conserved across dsDNA viruses A number of additional features of CDV-resistance remain uncharacterized CDV ... total of 10 intergenic and 45 intragenic SNPs, were Page of 15 observed that include 17 synonomous, 26 nonsynonomous substitutions and one tandem repeat contraction (Table 1) Over a third of all observed ... The effect of CDV on the viral and cellular RNA polymerase machinery has not been evaluated It is possible that viral RNA pol may be subject to either direct or indirect effects of CDV via dCTP...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25