nuclear reprogramming and induced pluripotent stem cells

... embryonic stem cells, ” Stem Cells and Development, vol 20, no 9, pp 1587–1599, 2011 [10] P Chatterjee, Y Cheung, and C Liew, “Transfecting and nucleofecting human induced pluripotent stem cells, ” ... embryonic stem cells (HESCs) and human -induced pluripotent stem cells (HIPSCs) to differentiate into cells with RPE morphology, function, and molecular phenotypes [4, 5] Thus far, HESC-, HIPSC- and ... epithelium from induced pluripotent stem cells, ” Stem Cells, vol 27, no 10, pp 2427– 2434, 2009 [5] J L Liao, J Yu, K Huang et al., “Molecular signature of primary retinal pigment epithelium and stem- cell-derived

Ngày tải lên: 01/11/2022, 09:48

... INTRODUCTION .1 1.1 Stem cells 1.1.1 Human embryonic stem 1.1.2 Induced pluripotent stem cells 1.2 History of stem cell culture technology ... Human Induced Pluripotent Stem Cells on Synthetic Substrate in Defined Medium" (2012) Theses and Dissertations 555 http://scholarworks.uark.edu/etd/555 This Thesis is brought to you for free and ... Theses and Dissertations 12-2012 Expansion of Human Induced Pluripotent Stem Cells on Synthetic Substrate in Defined Medium Huantong Yao University of Arkansas, Fayetteville Follow this and additional

Ngày tải lên: 04/11/2022, 07:33

... retinal cells from normal mouse and human fibroblast-derived induced pluripotent stem cells (iPSCs) (A–F): Microscopic analysis of mouse (A–C) and human (D–F) iPSC-retinal precursor cells generated ... picked, passaged, and clonally expanded on fresh Synthemax plates for further experimentation During reprogramming and maintenance of pluripotency, cells were cultured at 5% CO2, 5% O2, and 37°C iPSC ... Fungizone [Gibco], ng/ml noggin [R&D Systems], ng/ml Dkk-1 [R&D Systems], ng/ml insulin-like growth factor-1 [IGF-1] [R&D Systems], and 0.5 ng/ml bFGF [R&D Systems]), and plated at a density of ∼50 cell

Ngày tải lên: 18/10/2022, 01:27

... Human pluripotent stem cells Cardiomyocytes Doxorubicin Toxicity Biomarkers Introduction Abbreviations: cTnT, cardiac specific troponin T; hESC, human embryonic stem cells; hiPSC, human induced pluripotent ... doxorubicin -induced toxicity in human cardiomyocytes derived from pluripotent stem cells Gustav Holmgren a,b, * , Jane Synnergren a , Yalda Bogestål a,1, Caroline Améen c , Karolina Åkesson c, Sandra ... troponin T; hESC, human embryonic stem cells; hiPSC, human induced pluripotent stem cells; hPSC, human pluripotent stem cells; LDH, lactate dehydrogenase; SAM, statistical analysis of microarray

Ngày tải lên: 02/11/2022, 11:39

... embryo contains pluripotent stem cells (PSCs) that have developmental capacities equivalent to those of embryonic stem (ES) cells and induced pluripotent stem cells Mouse embryonic DRG cells expressed ... embryonic dorsal root ganglia contain pluripotent stem cells We found significant similarities between pluripotent stem cells and neural crest-derived stem cells ABSTRACT In the present study, ... The DRG cells expressed Oct4 (Fig 1B, E, G, J, L, and O), Sox2 (Fig 1C and E), Nanog (Fig 1H and J) and/ or SSEA1 (Fig 1M and O) Furthermore, the DRGs contained cells expressing both Oct4 and Sox2

Ngày tải lên: 26/07/2023, 07:38

... ENGINEERING: ENDOTHELIAL PROGENITOR CELLS AND HUMAN MESENCHYMAL STEM CELLS COCULTURE IN 3D HONEYCOMB SCAFFOLDS AND THE EFFECT OF BI-ROTATIONAL BIOREACTOR AND HYPOXIC MICROENVIRONMENT LIU YUCHUN ... Joint Symposium on Stem Cells and Bioimaging, 2425 May 2010, Singapore Y Liu, SH Teoh, SK Chong, Z Zhang, MA Choolani, J Chan Human endothelial progenitor stem cells accelerates and potentiates ... ideas, their patience and understanding, and the many hours they have each dedicated to sit down with me in person to guide and advise me in scientific thinking, planning, writing and making presentations

Ngày tải lên: 09/09/2015, 10:16

... ỨNG (INDUCED PLURIPOTENT STEM CELL – iPSC) HÀ NỘI - 2019 BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ Y TẾ TRƯỜNG ĐẠI HỌC Y HÀ NỘI ======= ĐẶNG THỊ HUYỀN NHUNG CHUYÊN ĐỀ TẾ BÀO GỐC VẠN NĂNG CẢM ỨNG (INDUCED PLURIPOTENT ... ỨNG (INDUCED PLURIPOTENT STEM CELL – iPSC) Chuyên ngành: Mô – Phôi Thai Học Mã số: 62720102 HÀ NỘI – 2019 DANH MỤC CHỮ VIẾT TẮT VÀ THUẬT NGỮ iPSC : Induced pluripotent stem cell – Tế bào gốc vạn ... tạo từ việc ni cấy ngun bào sợi có bổ sung thêm vài yếu tố gọi tế bào gốc vạn cảm ứng (Induced pluripotent stem cell – iPSC) [2] Việc đời tế bào gốc vạn cảm ứng mang đến hứa hẹn tuyệt vời cho liệu

Ngày tải lên: 09/08/2019, 09:32

... of stem cells, including embryonic stem cells (ESCs) and adult stem cells (ASCs), and focuses primarily on induced pluripotent stem cells (iPSCs) The key methods used for converting somatic cells ... and colleagues Phenotype and developmental potential of cardiomyocytes from induced pluripotent stem cells and human embryonic stem cells In: Ainscough J et al eds Nuclear reprogramming and stem ... and colleagues Phenotype and developmental potential of cardiomyocytes from induced pluripotent stem cells and human embryonic stem cells In: Ainscough J et al eds Nuclear reprogramming and stem

Ngày tải lên: 19/03/2021, 23:02

... cells in a mature tumor and the cell of origin These stem- like cells are commonly referred to as cancer stem cells (CSCs), brain tumor stem cells (BTSCs), or tumor-initiating cells For the purposes ... cell of origin models and clinical evidence for stem cells as the cells of origin in glioma NEUROGENESIS IN THE ADULT BRAIN Neural stem cells and their progeny have become candidates for the cell ... and human induced pluripotent stem cells (hiPSCs) are promising sources for hematopoietic cells This review summarizes recent advances in differentiating hESCs and hiPSCs to dendritic cells (DCs),

Ngày tải lên: 18/10/2022, 22:39

... CELL REPROGRAMMING: INDUCED PLURIPOTENT STEM CELLS The newest and, to many, the most exciting alternative to hESC research is the development of pluripotent cells through somatic stem cell reprogramming ... Pluripotent Stem Cells by Combining Genetic Reprogramming and Chemical Inhibitors, CELL STEM CELL 16, 18–19 (2009); Keisuke Okita et al., Generation of Mouse Induced Pluripotent Stem Cells Without ... Derivation of Human Embryonic Stem Cells from Developing and Arrested Embryos, 24 STEM CELLS 2669, 2670 (2006) 2011] PLURIPOTENT STEM CELLS 729 as ethical concerns over whether and when death actually

Ngày tải lên: 30/10/2022, 18:09

... Seandel, M., Geijsen, N., and Hochedlinger, K (2011) Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice Cell Stem Cell 9, 317–329 Bossard, P., and ... culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs hFSCs can selfrenew while maintaining their capacity to differentiate into pancreatic and hepatic cells ... population For example, neuronal stem cells can be easily expanded from human induced pluripotent stem cell (hIPSC) lines differentiated toward the neuroectoderm lineage and then differentiated into

Ngày tải lên: 02/11/2022, 10:44

... for making induced pluripotent stem cells: reprogramming a la carte Nat Rev Genet 2011, 12:231-242 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... Teitell MA, Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini...

Ngày tải lên: 11/08/2014, 12:21

... (Figure and 3): Oct4, Sox2, Klf4, and c-Myc (OSKM)48 These converted cells, which share morphological and pluripotent characteristics with ESCs, are known as induced pluripotent stem cells (iPSCs) ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs),...

Ngày tải lên: 09/09/2015, 18:56

... and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F, Beard C, Gao Q, ... CT: Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki...

Ngày tải lên: 11/08/2014, 12:21

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent ... thigh region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers...

Ngày tải lên: 30/09/2015, 10:13

... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... deletions and duplications [63-64], somatic coding mutations [65], and chromosomal duplications [66] hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent...

Ngày tải lên: 11/08/2014, 12:21

... Human Pluripotent Stem Cells Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and ... human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, ... karyotyping and fluorescent in situ hybridization (FISH) methods as they apply to the field of pluripotent stem cells Induced pluripotent stem cells attract great interest for their potential in understanding...

Ngày tải lên: 22/03/2014, 09:20

... GFP tag was cultured and allowed to differentiate so that there will be a mixture of stem cells and differentiated cells CDy9 was added to this semi-differentiated cells and it was observed that ... 10: CDy9 and immunocytochemistry staining of a mixture of differentiated cells and GFP-miPSCs (a) and (b) are 10X and 40 X images respectively CDy9 signal is only noticed for the stem cells which ... selectively stains mouse pluripotent stem cells only As such it was applied in mouse abdominal fats to isolate out any potential pluripotent- like stem cells It was noticed that round stem cell-like colonies...

Ngày tải lên: 02/10/2015, 17:14

... Hochedlinger, and G Q Daley Disease-specific induced pluripotent stem (iPS) cells 2008 Cell 134(5): 877–886 9) N Maherali, K Hochedlinger Guidelines and Techniques for the Generation of Induced Pluripotent ... mechanisms and functions can be deduced This will hence give a clearer picture on the compound staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent ... analysis and reculture Since the amount of pluripotent stem cells present in the body are very minimal, CDy9 may therefore aid in isolating just one pluripotent stem cell from the body and this...

Ngày tải lên: 02/10/2015, 17:14

... function (1 - 3) Typically, stem cells can be broadly classified into main groups, the embryonic stem cells and the somatic or adult stem cells The latter, adult stem cells are found in various ... embryonic stem cells (hESC) Until recently, another type of stem cells known as induced pluripotent stem cells (iPSC) have been made available to overcome these ethical concerns (7) These cells are ... of pluripotent stem cells These EBs were allowed to differentiate for days so that they consist of both pluripotent cells and differentiated cells These cells were seeded in 384 well plates and...

Ngày tải lên: 02/10/2015, 17:14